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1% Starch Solution Preparation

This document outlines the procedure to prepare a 100 mL 1% starch solution, which involves measuring 1 gram of starch, creating a slurry, heating water, and combining the two while stirring. The mixture is boiled until clear, cooled, and adjusted to the final volume. It is advised to use the solution fresh or store it in the refrigerator for up to 24 hours if not used immediately.

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1K views2 pages

1% Starch Solution Preparation

This document outlines the procedure to prepare a 100 mL 1% starch solution, which involves measuring 1 gram of starch, creating a slurry, heating water, and combining the two while stirring. The mixture is boiled until clear, cooled, and adjusted to the final volume. It is advised to use the solution fresh or store it in the refrigerator for up to 24 hours if not used immediately.

Uploaded by

sultanaafridi313
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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To prepare 100 mL of a 1% starch solution, follow this step-by-step procedure:

🌾 Ingredients and Materials:

 Starch powder (soluble starch or corn starch)


 Distilled water
 Beaker (100–250 mL)
 Glass rod for stirring
 Hot plate or water bath
 Measuring cylinder
 Funnel and filter paper (optional)

📏 What does 1% w/v mean?

 1% w/v = 1 gram of starch in 100 mL of solution

🧪 Procedure:

1. Weigh starch:
Measure 1 gram of starch powder.
2. Make a paste (to avoid clumping):
In a small beaker, add 10 mL of cold distilled water to the starch and stir it to form a
smooth slurry or paste.
3. Heat remaining water:
Heat 90 mL of distilled water to near boiling (~90°C) using a hot plate or water bath.
4. Add paste to hot water slowly:
While stirring continuously, add the starch slurry to the hot water. Keep stirring.
5. Continue heating and stirring:
Boil the mixture for 3–5 minutes until the solution turns clear or slightly
translucent, indicating the starch is gelatinized.
6. Cool and adjust volume:
Let the solution cool to room temperature, and transfer it to a 100 mL volumetric
flask or measuring cylinder.
Add distilled water to make the total volume exactly 100 mL.
7. (Optional) Filter:
If any undissolved particles remain, you may filter the solution using filter paper.

💡 Notes:

 Use the solution fresh, as starch solutions can degrade or become


contaminated over time.
 Store in the refrigerator if not using immediately, and use within
24 hours for enzyme assays.

Let me know if you're using this for amylase testing, Ulfat, and I can guide further on
controls and reaction setup.

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