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BT CT

The document outlines the procedures for determining bleeding time using Duke’s method and clotting time using Wright’s method, including the necessary apparatus and steps involved. It provides normal ranges for both tests and lists factors that can prolong bleeding and clotting times, such as platelet count and certain medical conditions. Additionally, alternative methods for measuring bleeding time are mentioned.

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Mukesh Swami
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140 views2 pages

BT CT

The document outlines the procedures for determining bleeding time using Duke’s method and clotting time using Wright’s method, including the necessary apparatus and steps involved. It provides normal ranges for both tests and lists factors that can prolong bleeding and clotting times, such as platelet count and certain medical conditions. Additionally, alternative methods for measuring bleeding time are mentioned.

Uploaded by

Mukesh Swami
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Determination of Bleeding Time and Clotting Time

BLEEDING TIME
AIM : To determine the bleeding time by Duke’s method.

APPARATUS REQUIRED

Filter paper, stop watch, lancet, cotton, spirit.

PROCEDURE
1. The tip of the left ring finger is pricked with aseptic precautions (3–4 mm).
2. The blood should flow freely without squeezing.
3. The time of puncture is noted.
4. With a filter paper the blood is gently blotted every 30 seconds.
5. The successive blots become smaller.
6. Thisprocedure is repeated until no blot appears on the filter paper.
7. The time is noted again.
8. The number of blots on the paper is counted.
9. Number of blots × 30 seconds will be the bleeding time.

Normal Range -1–5 minutes (by Duke’s method)

The factors which affect the bleeding time are:


1. Size and nature of the injury
2. Condition of the vessel wall
3. Number of platelets.

Conditions where bleeding time is prolonged:


1. Decrease in the number of platelets (Thrombocytopenia).
2. Functional platelet defect.
a. Drugs like aspirin, penicillin
b. von Willebrand’s disease
c. Uremia, cirrhosis, leukemia
3. Vessel wall defects.
a. Prolonged treatment with corticosteroids
b. Allergic purpuras
c. Infections with hemolytic streptococci, bacterial endocarditis
d. Deficiency of vitamin C
e. Senile purpura.

OTHER METHODS

1. In Duke’s method the edge of the ear lobe can also be used.

2. Ivy’s method: The cuff of the BP apparatus is applied to the upper arm and the
pressure is raised to 40 mm Hg. The front of the forearm is used. The normal bleeding
time by this method is up to 9 min.
CLOTTING TIME

AIM : To determine the clotting time by Wright’s method.

APPARATUS REQUIRED

Capillary glass tube 15 cm long with a bore of 0.8 mm, stop watch, lancet, cotton and
spirit

PROCEDURE
1. The tip of the left ring finger is pricked under aseptic precautions and the time of
puncture is noted.
2. The prick must be deep enough to allow free flow of blood without squeezing.
3. The blood is drawn into the capillary tube by dipping one end of the tube in the
blood drop.
4. The blood fills the tube by capillary action.
5. After 2 minutes, a small bit of the tube is broken every 30 seconds until a fine
thread of fibrin appears between the broken ends.
6. The time is again noted and the interval between the prick and the appearance of
fibrin thread gives the clotting time.

Normal Range - 2–8 minutes.

Conditions where clotting time is prolonged


1. Hemophilia (Bleeding time is normal)
2. Christmas disease
3. Afibrinogenemia
4. Vitamin K deficiency
5. Liver disease
6. Anticoagulant therapy
7. New born baby.

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