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Collection and Storage of Saliva

The document outlines the proper collection and storage procedures for saliva samples at a crime scene. Saliva should be collected from items like cigarettes or utensils, ensuring they are dried and stored in air-permeable containers to maintain evidentiary value. For storage, samples should ideally be frozen at -20 ºC or lower to prevent degradation, with alternative short-term storage options provided.

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Gogul Ramnath
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110 views2 pages

Collection and Storage of Saliva

The document outlines the proper collection and storage procedures for saliva samples at a crime scene. Saliva should be collected from items like cigarettes or utensils, ensuring they are dried and stored in air-permeable containers to maintain evidentiary value. For storage, samples should ideally be frozen at -20 ºC or lower to prevent degradation, with alternative short-term storage options provided.

Uploaded by

Gogul Ramnath
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Collection procedure of saliva in crime scene

• Saliva is typically found on a substrate, most often a cigarette, sealed


envelope, stamp, or eating/drinking utensil.

• ​If the entire item bearing the saliva stain can be collected, collect the entire
item.

• If the saliva stain is wet, place the item on new, clean, dry paper. Place the
item and paper in a draft-free, dry location that is secure, ensuring no one
will touch, step on, remove, or displace the item. Allow the item to air dry.
Do not package the item when wet; it may putrefy and therefore lose its
evidentiary value.

• When the item is dry, insert it into an appropriately sized, air-permeable but
closed container, such as a cardboard box, paper envelope, or paper bag.
Do not fold the item unless it is absolutely necessary. If the item must be
folded, protect the stained area with a piece of paper and avoid folding
across the stained area.
• If the saliva is on an immovable surface, like a tile floor, it can be collected
using an absorbent medium, such as a new, clean, sterile cotton gauze pad

Storage of saliva sample

• If the analysis is to be performed immediately, specimens can be stored at


room temperature (maximum 30-90 min)

• Lowering the incubation temperature lowers the degradation rate of salivary


proteome. Thus, immediately after saliva collection, it is recommended to
freeze the samples at or below -20 ºC. If a freezer is not available,
specimens can be stored at 4 ºC to prevent bacterial growth and further
degradation of salivary molecules (no longer than 6 h). Specimens can also
be stored at -80 ºC for several years with little or no degradation

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