Post RN BS Nursing Final Terminal Examination 2025

Year: 1 Semester: - I (Session 2024– 2026)

Objective Paper: Biochemistry
Note: -Marking with Led pencil & Double attempts of one objective shall not be
considered.
Multiple choices Question, Encircle is the best option for the followings.

1. What is the general formula of carbohydrates?

A. CnH2nOn
B. CnH2n-2On
C. CnHnOn
D. CH2O
2. Which of the following is a monosaccharide?
A. Sucrose
B. Lactose
C. Fructose
D. Maltose
3. Glucose is an example of a:
A. Disaccharide
B. Polysaccharide
C. Monosaccharide
D. None
4. The bond formed between two monosaccharides is called:
A. Peptide bond
B. Glycosidic bond
C. Hydrogen bond
D. Ester bond
5. Which polysaccharide is found in plant cell walls?
A. Glycogen
B. Cellulose
C. Starch
D. Chitin
6. Glycogen is stored in the human:
A. Brain
B. Liver and muscles
C. Heart
D. Blood
7. What enzyme breaks down starch into maltose?
A. Lipase
B. Amylase
C. Maltase
D. Lactase
8. Which sugar is found in milk?
A. Glucose
B. Lactose
C. Sucrose
D. Fructose
9. Hydrolysis of sucrose yields:
A. Glucose + glucose
B. Glucose + fructose
C. Glucose + galactose
D. Fructose + fructose
10. Which of the following is not a function of carbohydrates?
A. Energy source
B. Energy storage
C. Structural support
D. Enzyme activity
11. What is the storage form of carbohydrates in animals?
A. Starch
B. Glycogen
C. Cellulose
D. Glucose

12. What is the sweetest natural sugar?

A. Glucose
B. Fructose
C. Sucrose
D. Lactose
13. Which of the following is a reducing sugar?
A. Sucrose
B. Glucose
C. Starch
D. Cellulose
14. Which test is used to detect reducing sugars?
A. Benedict's test
B. Iodine test
C. Biuret test
D. Sudan III test
15. Which polysaccharide is used for energy storage in plants?
A. Glycogen
B. Cellulose
C. Chitin
D. Starch
16. What is the color change in iodine test for starch?
A. Blue-black
B. Red
C. Yellow
D. Green
17. Which carbohydrate is non-digestible in humans?
A. Glycogen
B. Starch
C. Cellulose
D. Sucrose
18. Maltose consists of:
A. Two glucose molecules
B. Glucose and galactose
C. Glucose and fructose
D. Two fructose molecules
19. Which enzyme breaks down lactose?
A. Amylase
B. Lactase
C. Maltase
D. Sucrase
20. What is the function of insulin in carbohydrate metabolism?
A. Raises blood sugar
B. Breaks down glucose
C. Lowers blood sugar
D. Converts proteins to sugar
21. Which sugar is present in RNA?
A. Ribose
B. Deoxyribose
C. Fructose
D. Glucose
22. Dehydration synthesis of two monosaccharides forms:
A. Protein
B. Lipid
C. Disaccharide
D. Amino acid
23. In glycolysis, glucose is broken down into:
A. Pyruvate
B. Acetyl-coA
C. Lactic acid
D. Carbon dioxide
24. The first step of glycolysis is:
A. Glucose oxidation
B. Glucose phosphorylation
C. Pyruvate production
D. Acetyl-CoA formation
25. Which enzyme is important in glycolysis?
A. Hexokinase
B. Lipase
C. Protease
D. DNA polymerase
Proteins (25 MCQs)
26. Proteins are made up of:
A. Fatty acids
B. Monosaccharides
C. Nucleotides
D. Amino acids
27. A peptide bond is formed between:
A. Two sugars
B. Two fatty acids
C. Two amino acids
D. A sugar and a base
28. The basic unit of a protein is:
A. Glucose
B. Nucleotide
C. Amino acid
D. Glycerol
29. The primary structure of a protein is:
A. Sequence of amino acids
B. Alpha helix
C. Beta sheets
D. Polypeptide chain folded
30. Which of the following is a fibrous protein?
A. Hemoglobin
B. Insulin
C. Keratin
D. Albumin
31. What is the function of enzymes?
A. Energy storage
B. Cell structure
C. Speed up reactions
D. Hormone transport
32. What type of bond stabilizes the secondary structure of proteins?
A. Peptide bonds
B. Ionic bonds
C. Hydrogen bonds
D. Disulfide bridges
33. Which of the following is a conjugated protein?
A. Hemoglobin
B. Keratin
C. Actin
D. Collagen
34. Denaturation of proteins involves:
A. Breaking peptide bonds
B. Loss of 3D structure
C. Formation of new bonds
D. Hydrolysis
35. Which test is used to detect proteins?
A. Benedict’s test
B. Biuret test
C. Iodine test
D. Sudan III test
36. The R-group in amino acids determines:
A. Acid strength
B. Basicity
C. Specific properties
D. Chain length
37. Which of the following is an essential amino acid?
A. Glycine
B. Valine
C. Alanine
D. Serine
38. Enzymes are:
A. Lipids
B. Nucleic acids
C. Proteins
D. Carbohydrates
39. Enzymes lower the:
A. Product concentration
B. Temperature
C. Activation energy
D. pH
40. The tertiary structure is stabilized by:
A. Ionic and hydrogen bonds
B. Only peptide bonds
C. Glucose
D. Water
41. Which vitamin is protein-based?
A. Vitamin A
B. Vitamin D
C. None
D. All proteins are not vitamins
42. What happens when proteins are hydrolyzed?
A. Form fatty acids
B. Break into amino acids
C. Make sugars
D. Produce glycerol
43. Which enzyme digests protein in the stomach?
A. Pepsin
B. Amylase
C. Lipase
D. Trypsin
44. Enzymes work best at:
A. High temperature
B. Specific pH and temperature
C. Cold temperatures
D. High pressure
45. Which of the following is a complete protein?
A. Wheat
B. Rice
C. Egg
D. Corn
46. What is a dipeptide?
A. 2 fatty acids
B. 2 sugars
C. 2 amino acids
D. 2 nucleotides
47. What is the quaternary structure?
A. Single chain
B. Double helix
C. Multiple polypeptides
D. Alpha helices only
48. What is the enzyme’s active site?
A. The whole enzyme
B. Binding region
C. Substrate
D. Product area
49. Which level of protein structure is affected by denaturation?
A. Primary
B. Secondary
C. Tertiary
D. Quaternary
50. Which enzyme works in small intestine to digest proteins?
A. Trypsin
B. Lipase
C. Sucrase
D. Pepsin
Lipids (25 MCQs)
51. Lipids are composed of:
A. Amino acids
B. Sugars
C. Glycerol and fatty acids
D. Nucleotides
52. Which of these is not a lipid?
A. Phospholipid
B. Cholesterol
C. Glucose
D. Triglyceride
53. Saturated fats contain:
A. No double bonds
B. One double bond
C. Many double bonds
D. No hydrogen
54. Unsaturated fats are usually:
A. Solid at room temperature
B. Liquid at room temperature
C. Gaseous
D. None
55. Triglycerides consist of:
A. 3 glycerols + 1 fatty acid
B. 3 fatty acids + 1 glycerol
C. 1 fatty acid + 3 sugars
D. 1 amino acid + 3 fatty acids
56. Phospholipids are major components of:
A. Nucleus
B. Cytoplasm
C. Cell membranes
D. Ribosomes
57. Which lipid is a steroid?
A. Cholesterol
B. Glycerol
C. Palmitic acid
D. Lecithin
58. Waxes are a type of:
A. Protein
B. Lipid
C. Carbohydrate
D. Vitamin
59. Lipids are soluble in:
A. Water
B. Alcohol
C. Organic solvents
D. Both B & C
60. Which test detects fats?
A. Biuret
B. Benedict's
C. Sudan III
D. Iodine
61. The main function of lipids is:
A. Genetic material
B. Energy storage
C. Enzyme production
D. Structure of DNA
62. Which of these is not a function of lipids?
A. Insulation
B. Hormone synthesis
C. Energy
D. DNA synthesis
63. Lipid molecules are:
A. Hydrophilic
B. Hydrophobic
C. Amphipathic
D. Ionic
64. Cholesterol is essential for:
A. Blood sugar
B. Cell membranes
C. DNA synthesis
D. Enzyme activation
65. Lipid digestion begins in:
A. Mouth
B. Stomach
C. Small intestine
D. Liver
66. Which enzyme breaks down lipids?
A. Pepsin
B. Amylase
C. Lipase
D. Protease
67. Bile salts help in:
A. Digestion of proteins
B. Emulsification of fats
C. Digestion of sugars
D. Absorption of water
68. The lipid bilayer is found in:
A. DNA
B. Enzymes
C. Cell membranes
D. Ribosomes
69. Which lipids are precursors of hormones?
A. Waxes
B. Phospholipids
C. Steroids
D. Oils
70. Fat-soluble vitamins include:
A. B and C
B. A, D, E, K
C. D and B
D. C and E
71. Which fat is heart-healthy?
A. Saturated fat
B. Trans fat
C. Unsaturated fat
D. Animal fat
72. Trans fats are harmful because they:
A. Lower LDL
B. Increase HDL
C. Raise LDL and lower HDL
D. Are natural
73. Which of the following is an omega-3 fatty acid source?
A. Butter
B. Fish oil
C. Cheese
D. Red meat
74. Which organ produces bile?
A. Pancreas
B. Liver
C. Gallbladder
D. Stomach
75. Which of these is a lipid-based hormone?
A. Insulin
B. Estrogen
C. Pepsin
D. Trypsin
76. Which organ is primarily responsible for regulating blood glucose levels?
A. Kidney
B. Liver
C. Pancreas
D. Intestine
77. Which of the following lipoproteins is considered "good cholesterol"?
A. VLDL
B. LDL
C. HDL
D. Chylomicrons
78. What is the primary site for fatty acid synthesis?
A. Mitochondria
B. Cytoplasm
C. Endoplasmic reticulum
D. Golgi apparatus
79. Transamination is the process of:
A. Adding an amino group
B. Removing an amino group
C. Transferring an amino group
D. Breaking a peptide bond
80. Which hormone stimulates lipolysis?
A. Insulin
B. Glucagon
C. Thyroxine
D. Estrogen
81. Which of the following carbohydrates is a non-reducing sugar?
A. Maltose
B. Lactose
C. Sucrose
D. Glucose
82. Which essential amino acid is needed for growth in infants?
A. Arginine
B. Glycine
C. Serine
D. Alanine
83. Ketone bodies are formed from excessive breakdown of:
A. Carbohydrates
B. Amino acids
C. Fatty acids
D. Nucleic acids
84. Which of these is a branched-chain amino acid?
A. Glycine
B. Leucine
C. Lysine
D. Glutamine
85. Which vitamin is necessary for proper metabolism of amino acids?
A. Vitamin C
B. Vitamin B6
C. Vitamin A
D. Vitamin K
86. In protein structure, disulfide bonds form between:
A. Lysine and arginine
B. Cysteine residues
C. Methionine and leucine
D. Serine and threonine

87. What is the storage form of carbohydrates in humans?

A. Starch
B. Glycogen
C. Cellulose
D. Maltose
88. Which process generates urea?
A. Lipolysis
B. Beta-oxidation
C. Urea cycle
D. Glycolysis
89. The final product of beta-oxidation of even-chain fatty acids is:
A. Lactate
B. Acetyl-CoA
C. Pyruvate
D. Ketone bodies
90. Which of the following proteins has a quaternary structure?
A. Myoglobin
B. Hemoglobin
C. Insulin (monomer)
D. Pepsin
91. In glycolysis, one molecule of glucose is converted into:
A. One pyruvate
B. Two pyruvates
C. Two acetyl-CoA
D. One lactic acid
92. Which amino acid is the precursor of serotonin?
A. Tyrosine
B. Phenylalanine
C. Tryptophan
D. Glutamine
93. Lipids are transported in the blood as:
A. Free fatty acids
B. Triglycerides
C. Lipoproteins
D. Glycerol
94. Which of the following enzymes is required for the digestion of proteins in the stomach?
A. Trypsin
B. Pepsin
C. Amylase
D. Lipase
95. Which type of fatty acid contains no double bonds?
A. Monounsaturated
B. Polyunsaturated
C. Saturated
D. Trans
96. Which metabolic pathway occurs in both the cytoplasm and mitochondria?
A. Glycolysis
B. TCA cycle
C. Urea cycle
D. Electron transport chain
97. A positive Benedict’s test indicates the presence of:
A. Proteins
B. Lipids
C. Reducing sugars
D. Non-reducing sugars
98. Which component is common to both triglycerides and phospholipids?
A. Fatty acids
B. Cholesterol
C. Phosphate group
D. Nitrogen
99. Which amino acid contains sulfur?
A. Leucine
B. Serine
C. Cysteine
D. Glycine
100. The bond linking amino acids in a protein is a:
A. Glycosidic bond
B. Ester bond
C. Peptide bond
D. Hydrogen bond

KEY
1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
B C A B D A C D A B
11. 12. 13. 14. 15. 16. 17. 18. 19. 20.
D B C B D B C A D C
21. 22. 23. 24. 25. 26. 27. 28. 29. 30.
C D B A B A C B B A
31. 32. 33. 34. 35. 36. 37. 38. 39. 40.
A D B B D A A C D C
41. 42. 43. 44. 45. 46. 47. 48. 49. 50.
C B A A B A C B A D
51. 52. 53. 54. 55. 56. 57. 58. 59. 60.
B C C C D B A C A D
61. 62. 63. 64. 65. 66. 67. 68. 69. 70.
C B C A D A A B C D
71. 72. 73. 74. 75. 76. 77. 78. 79. 80.
B A C C A B C B C B
81. 82. 83. 84. 85. 86. 87. 88. 89. 90.
C A C B B B B C B B
91. 92. 93. 94. 95. 96. 97. 98. 99. 100.
B C C B C C C A C C

Subjective

Q1 Describe the main types of carbohydrates?

Q2 What do you know about the poly saccharine?
Q3 Write down the difference type of protein with function in human body
Q4 Write a short note on entomology.
Q5 Write the metabolism of carbohydrates in human body
Q6 Whatever do you know about lipid? Also writes its types?
Q7 What do you know about the hydrogen cycle?
Q8 Write a brief note on Glycogenolysis.
Q9 Write down the function of different Enzymes in human body.

The End