DEDEPARTMENT OF BIOCHEMISTRY, SVNGMCYAVATNMAL
PRELIMINARY EXAMINATION OF FIRST MBBS 2024 BATCH
BIOCHEMISTRY THEORY - PAPER II
Date - Time - 3 Hours Marks - (100)
SECTION-A-MCQ
INSTRUCTIONS: - 1) AIIMCQs are compulsory. (20 Marks]
2)Each MCQ carries 1 mark
3) No negative marking. No overwriting or double answer allowed
1) A70 years old male is admitted in ICUwith complaints of increased micturition and pain in abdomen. He
was a known case of chronic renal failure. Which investigation techniques you will advise for separation of
proteins ?
a) Electrophoresis b) Flame Photometry c) Calorimetry d) Chromatography
2) Which of the following amino acid pair serves as precursor for the synthesis of both glucose and fat?
a) Alanine &Aspartate b) Leucine &Methionine c) Tyrosine &Phenyialanine d) Methionine &Glycine
3) First line of compensatory mechanism to regulate hydrogen ion concentration in body is
la) Plasma Buffers b) Reclamation of HCO, ions c) Respiratory mechanisms d) Excretion of NH4 ions
4) Which of the following is miscellaneous nucleotide?
a) PAPS b) ATP c) GTP d) CTP
5) Uric acid is
a) 6-oxypurine b) 2,6-dioxypurine c) 2,6,8-trioxypurine d) 2,4-dioxypyrimidine
6) Serum gamma glutamyl transpeptidase is raised in
a) Haemolytic Jaundice b) Myocardial Infarction c) Alcoholic Hepatitis d) Acute Cholecystitis
7) Microalbuminuria is identified as
a) 0-300 gm/day b) 30-300 mg/day c) 03-30 mg/day d) None of the above
8) Buffering action of hemoglobin is mainly due to its residues
a) Glutamine b) Arginine c) Histidine d) Lysine
9) Polypeptide composition of HbF is
a) alpha 2 beta 2 b) alpha 2 gamma 2 c) alpha 2 delta 2 d) alpha 2 epsilon 2
10) Inherited deficiency of Purine Nucleoside Phosphorylase causes
a) Dwarfisn b) Mental Retardation c) Immunodefieiency d) Gout
11) Laboratory diagnosis of vitamin B12 deficiency can be made by measuring the urinary excretion of
a) Xanthurenic acid b) Formiminoglutamic acid c) MethyImalonic acid d) Homogentisate
12) Apoferritin molecule is made up of how many subunits?
a) 4 b) 8 c) 10 d) 24
13)A48-year-old factory worker on hunger strike since 8 days landed up in metabolic acidosis. Mention the
mineral likely to be raised in plasma in above case.
a) Sodium b) Potassium c) Calcium dOPhosphorus
14) Unusual nucleotide pseudouridylic acid is present in
a) mRNA b) tRNA c) rRNA d) hnRNA:
15)Which of the following is a correct description of the chemistry of DNA and RNA?
a Uracil is a purine base that occurs in RNA but not in DNA
b. Cytosine is a pyrimidine base that occurs in both RNA and DNA
C Ribose is a pentose sugar that occurs in DNA and RNA
d. Thymine is a pyrimidine base that occurs in RNA but not in DNA.
16) A cosmid is a
a) Large Bacterial Plasmid b) Viral Plasmid c) Hybrid of plasmid and phage d) Yeast plasmid
17) DNA finger printing is based on the presence in DNA of
a) Constant Number of Tandem Repeats b) Variable number of tandem repeats
c) Non-repetitive sequences in each DNA d) Introns in eukaryotic DNA
18) Aparticular RNA in amixture can be identified by which blot technique?
a) Western b) Eastern c) Northern d) Southern
19) Which of the following is used as a label in ELISA?
a) Antibody b) Antigen ¿) Enzyme d) Radioisotope
20)Which of the following chromatographic techniques is based on molecular size?
a) Gel filtration chromatography b) lon exchange chromatographý
c) Paper chromatography d) Affinity chromatography
DEPARTMENT OF BIOCHEMISTRY, SVNGMC YAVATMAL
PRELIMINARY EXAMINATION OF FIRST MBBS 2024 BATCH
BIOCHEMISTRY THEORY Paper Il
SECTIONB
Note: Write answers in Sequence. Draw diagrams or Flow chart wherever necessary
Que 2. Long Answer Questions) (1 LAQ 12 marks) [12 Marks]
A) Describe the pathway of urea formation in the body. Discuss the metabolic disorders that
can disrupt this pathway. Explain the clinical significance of elevated blood urea levels in
context of chronic renal failure. (6+3+3)
Que 3 Reasoning questions [5 questions 4 marks each] [20 Marks]
a) Explain Physiological functions of Zinc in the human body. How adeficiency of zinc can
lead to specific clinical manifestations by linking biological roles to observedsymptoms.
b) Explain how genetic engineering is applied in the field of medicine. Support your answer
with specific examples, and reason how these genetic modifications lead to
improvements in diagnosis, treatment, or prevention of diseases.
c) Discuss the biochemical functions of Pantothenic acid and analyze how its deficiency can
lead to specific clinical manifestations. Justify your answer by linking its metabolic roles to
the symptoms observed in deficiency states.
d) A 19-year-old girl is admitted to the medicine ward and diagnosed with anemia. Her lab
report reveals a hemoglobin (Hb) concentration of less than 7 gm%, indicating severe
anemia.ldentify the degradation compound involved in this condition & explain how it
contributes to development of anemia.(Its Origin, Metabolism, and Pathological role)
e) A 68-year-old male patient has been diagnosed with end-stage renal disease (ESRD)
Laboratory findings reveal a significantly decreased glomerular filtration rate (GFR), To
assess kidney function more accurately, the physician orders a creatinine clearance test.
Explain how the creatinine clearance test is performed to measure GFR. Additionally.
discuss the advantages of using the creatinine clearance test in evaluating renal function.
Que 4 Short Notes (any 4 out of 5) [5 marks each] [20 Marks]
a) Write in brief about regulation of gene expression.
b) Explain the principle, types, and clinical applications of the Enzyme-Linked Immunosorbent
Assay (ELISA) in biochemistry.
c) Write in brief Wobble's hypothesis and relationship of codon &: anticodon.
d) Explain in brief about the compensatory mechanism (hrmones) regulating the water
and electrolyte balance in case of hypovolumic shock.
e) Write in brief dietary sources, biochemical functions and deficiency manifestations of copper.
Que 5 Short Answer Questions (any 4 out of 5) [7 marks each] (28 Marks]
a) Explain the physician's role and responsibility to society and the community that he or
she serves.
b) Interpret the laboratory data: Van den Berg reaction- Direct Positive: Total Bilirubin
14.6 mg%: Direct Bilirubin 12.8 mg%: Indirect Bilirubin: 1.8 mg%: ALT 34 IUIL: AST:
30 IU/L: ALP 490 |U/L. Mention the probable diagnosis. WVhat can be the causative
factors in this condition?
c) Urine examination of a prisoner on hunger strike since 3 days was found to be
positive for ketone bodies. Mention the Ketogenic amino acids. Write the significance
of raised level of above parameter in starvation.
d) Describe dietary sources and deficiency manifestations of vitamin C.
e) Write the functional classification of protein with suitable example. Also write a note
on denaturation of proteins.
