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Role of Microbes in Plant Growth and Food Preservation: AGRINULA Jurnal Agroteknologi Dan Perkebunan June 2021

The article discusses the significant role of microbes in enhancing plant growth and food preservation. It highlights how microorganisms improve soil health, nutrient availability, and crop yields while also contributing to food preservation through fermentation processes. The review emphasizes the importance of understanding microbial interactions in agriculture to meet the demands of a growing global population.

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0% found this document useful (0 votes)
12 views17 pages

Role of Microbes in Plant Growth and Food Preservation: AGRINULA Jurnal Agroteknologi Dan Perkebunan June 2021

The article discusses the significant role of microbes in enhancing plant growth and food preservation. It highlights how microorganisms improve soil health, nutrient availability, and crop yields while also contributing to food preservation through fermentation processes. The review emphasizes the importance of understanding microbial interactions in agriculture to meet the demands of a growing global population.

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Role of Microbes in Plant Growth and Food Preservation

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DOI: 10.36490/agri.v4i2.158

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Review Article

Agrinula : Jurnal Agroteknologi dan Perkebunan


2021, vol. 4 (2): 106-121

website : https://journal.utnd.ac.id/index.php/agri
E-ISSN : 2655-7673
DOI : https://doi.org/10.36490/agri.v4i2.158

ROLE OF MICROBES IN PLANT GROWTH AND FOOD PRESERVATION

Aqarab Husnain Gondal1*, Qammar Farooq 1, Irfan Hussain1, & Muhammad


Danish Toor2

1
Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad
38040, Pakistan.
2
European University of Lefke, Institute of Graduate Studies and Research, Department
of Environmental Sciences Northern Cyprus TR-10 Mersin Turkey.
*Corresponding author: aqarabhusnain944@gmail.com

Article Information ABSTRACT


Submitted:  Introduction: Microbes perform better functions for
May 05, 2021 agricultural production by promoting various direct and
indirect mechanisms in soil and plants. If agricultural
Revised: development is to satisfy the needs of an increasing
June 13, 2021 global population, a deeper understanding of soil
microbiology is needed. Furthermore, microbial biota
Accepted: such as yeast, bacteria etc., plays a significant role in
June 15, 2021 food preservation by various mechanisms.
 Review results: Despite their pathogenicity, microbes
Published: play a substantial role in dispensing an assortment of
June 17, 2021 fermented drinks and foods in the food industry and
home. Probiotics, fermented foods and alcoholic
beverages are flattering extra popular due to their health
benefits and flavour. Furthermore, they increase the yield
and growth of plants by improving mineral availability
to the plants and by another mechanism.
 Review implementation: This present review also
discusses the various organisms used in the agricultural
processing of beverages and food and the benefits of
using the following microbes in the beverage and food
industry.
Keywords: crop growth and yield; food industry; food
preservation; microbes

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107

INTRODUCTION
The soil ecosystem is comprised of inorganic material (iron calcium, oxides,
sulfur, nitrates, phosphates, stone particles, and ash), substrates (dead organisms, fallen
leaves, dead roots, and rotten wood), organisms (microorganisms, plants, and animals),
water and air. It also provides food, fibre, fruit and shelter to all living organisms. The
use of agrochemicals in industries and agricultural production leads to contamination of
the environment that is a severe problem in the coming days. Furthermore, the world
population is increasing by leaps and bounds; thus, already used anthropogenic
activities are not sufficient to produce maximum food. Again, agricultural products that
are used in the industry become tasteless and poison due to microbial degradation.
Microbes play an essential role in plant growth and development in a variety of
pathway. Plant growth-promoting organisms are used to boost agricultural crop growth
and yields under natural and stressful conditions. It enhances the growth of crop plants
by improving a variety of physiological parameters in response to external stimulants
through various mechanisms. Plant growth regulators, the improvement of several
metabolites, and the conversion of atmospheric (O2) nitrogen into ammonia (NH4+) are
some of the mechanisms that can take part in the growth elevation of plants. It also
provides resistance from pathogens through induced systemic resistance and systemic
acquired resistance (Kumar & Verma, 2019).
Microorganisms are well known for their involvement in food spoilage. Still, they
also play a beneficial role in the food industry as they are helpful in the preservation of
food and dairy products, i.e. fermentation. In the process of fermentation,
microorganism are used, and they increase the shelf life of food. Lactic acid bacteria
(LAB) are used in several fermentation and preservation process as their metabolic
characteristics are unique. For example, milk, meats, cereals, and vegetables (Caplice &
Fitzgerald, 1999). The unique ability of LAB is a production of Bacteriocins. It is an
anti-microbial compound, so LAB is used as a preservation agent. In milk fermentation,
it is used as a “starter culture”, so it produces acid in the fermentation process of milk
(Rakhmanova et al., 2018). Microorganisms play a vital role in the growth of plants.
They live in the rhizosphere of plants, and it is an interface of plant-microbe where they
interact with the plants' roots, and many processes like exchange of materials and
signalling process occur (Qiao et al., 2017). There is a strong interaction between plants
and microorganisms living in the soil. They are involved in many essential mechanisms
for both communities of plants and microbes, e.g. neutral, synergistic or antagonistic
mechanisms. Among microorganisms living in the rhizosphere, plant growth-promoting
bacteria (PGPB) are most important because they stimulate the growth of plants by
several processes, i.e. nitrogen fixation, regulation of soil nutrients availability,
suppression of pathogens, and modify the response of plants for biotic and abiotic
stresses (Shameer & Prasad, 2018).
Hence the study of microbes towards agricultural production and food
preservation in the industry is essential. Previous studies show that microbes play a
crucial role in almost every field of life. The main objectives of this study are (a) to

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highlight the role of microbes in plant growth and development, (b) to demonstrate the
role of microbes in food industries.

RESULT REVIEW
Role of Microbes in Soil Development
The presence of microorganisms influences the formation and development of
soils. During soil formation, microorganisms produce several pores and crevices so that
roots of plants can easily penetrate the cracks and production of soil fragmentation is
increased. Many factors control the rate of soil development, and microorganisms are an
essential factor (Egli et al., 2011). Soil nutrients status is also determined by microbes
quality and their identification in the soil (Lombard et al., 2011). For soils' development,
microorganisms play a crucial role in improving soil fertility status and overall health.
Bacteria, actinomycetes, fungi, algae, protozoa, and viruses are all forms of soil
microorganisms. All organisms have unique traits that distinguish them from one
another and define their function in the soil. Importantly, these creatures do not exist in
isolation; they interact, and their interactions impact soil production equivalent to or
larger than the individuals' behaviour (Berg et al., 2010). Microorganisms are critical to
soil creation and soil ecology because they regulate the flux of nutrition to plants
(control of the biomass, sulphur, and nitrogen cycles), support nitrogen fixation, and
encourage the soil detoxification of inorganic material naturally stirring organic
contaminants. In addition, many bacteria exist in symbiotic relationships with plant
roots, creating a zone with the high microbial activity known as the rhizosphere (Zhu et
al., 2019).
The soil micro, meso, and macro-fauna have a beneficial association. For
example, protists, tiny nematodes, rotifers, tardigrades (microfauna) with a body size of
fewer than 100 m, springtails, detritivore, and predatory mites, proturans, symphylans
(mesofauna) with a body size of between 100 m and 2 mm, and insects such as ants,
carabids, termites, cicadas, and many species The majority of soil activities and
significant biogeochemical cycles are thought to be primarily governed by soil
microbial populations (FAO, 2017).
Earthworms, Nematodes, Acarina, Collembola, Mollusks, and various pests and
grubs feed primarily on decomposing organic materials and life. They're all part of the
soil food web, helping to move organic materials from the soil to fungal and bacterial
biomass. Soil microbiology is a young field, with just around 1% of soil microbes
described so far (Brown et al., 2000). Fungi and bacteria are heterotrophic organisms
that eat the micronutrients (zinc) and minerals released by decaying organic waste. As a
result, they're crucial to the nutrient recycling system that keeps soils healthy for plant
growth. One kind of soil's microorganism culture differs from another kind of soil's
microorganism culture (Bossio et al., 1998).
Microorganisms are present on soil surface and form “bio crust”. These bacteria
can alter soil chemical and biological features such as crucial nutrient cycling (i.e.
nitrogen and carbon), nitrogen and carbon fixation rates in soil, and soil fertility (Rossi

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et al., 2015, Rashid et al., 2016). Soil aggregate stability, bulk density, particle density,
water holding capacity, soil organic matter levels, carbon, and nitrogen are all affected
by soil microbial activity and density (Veum et al., 2014).
Microbes help form the physical structure of soils by secreting organic chemicals
(mostly sugar) that act as "bonding agents" in the soil, glueing soil particles together
and forming soil structure. Other soil physical qualities are influenced indirectly by soil
microbes; for example, improved soil structure increases soil porosity, leading to more
significant air and water penetration. Soil microbes also create extracellular
polysaccharides or other cellular waste, which aid in the preservation of soil structure
and soil health (Wani et al., 2015). Colica et al., (2014) also found that when
microorganisms (Cyanobacteria) released polysaccharides, soil structure improved, and
soil hydraulic conductivity decreased. According to Rossi & De Philippis, (2015) the
development of biofilms by cyanobacteria is beneficial in enhancing soil's physical and
chemical properties. For instance, soil surface particle adhesion and carbon
sequestration in soil.
Important activities microorganisms in soil include: (1) soil organic matter
mineralization and nutrient cycling (Miransari, 2011a), (2) soil structure improvement
(Egamberdiyeva, 2007), (3) positive interaction with other soil microorganisms
(Miransari, 2011b), (4) production of biochemicals like plant hormones and essential
enzymes (Compant et al., 2010), and (5) suppression of plant pathogens (Mendes et
al., 2011).
Nitrogen is present in our environment, but it is not available for plant use.
However, some specific bacteria can capture this atmospheric nitrogen and transfer it
into the plant-available form. These microorganisms are known as “nitrogen fixation
bacteria”. This microbial process is most important for plants and the overall
agricultural system because it reduces chemical nitrogen fertilizers dependence.
According to estimation, the biological nitrogen fixation process produces nitrogen 200
million tons per annum globally. Soil microorganisms like bacteria have a symbiotic
relationship, and these are also known as “rhizobia”, including
genera Rhizobium, Bradyrhizobium, Mesorhizobium, and Sinorhizobium, with the roots
of leguminous crops which generate nodules that are responsible for atmospheric
nitrogen fixation by nitrogenase enzyme activity (Graham & Vance, 2003).
Some other soil functions are also driven by the diverse set of soil microorganisms
contributing to functional resilience like decomposition. Moreover, other relevant soil
functions are involved in more specific set of soil microorganisms, for example,
nitrifiers and bio-control agents. It has been observed that the makeup of the soil
organism community can affect numerous activities in the soil ecosystem, including
plant variety, decomposition, nutrient retention, and cycling (Semhi et al., 2008). These
micro and macro-organisms work together to improve soil quality. Macrofaunal
creatures play an essential role in the soil system as colonisers, comminutors, and
engineers, and their interactions with microbes are critical for this (Sofo et al., 2020).

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The most effective bio-agent for improving soil health and fertility are the soil
microbes.

Role of Microbes in Plant Growth and Yield


The soil microbial dynamics have a crucial role in determining the soil's ability to
create crop yield. They change the pH of the earth by numerous factors, resulting in the
absorption of a variety of macro and micronutrients, particularly zinc (Gondal et al.,
2021a; 2021b; 2021c; 2021d). While plant-microbe interactions are an essential aspect
in controlling ecosystem functioning, they vary significantly and are influenced by
nutrient availability. Plant growth-promoting microorganisms are primarily employed to
stimulate plant development via many methods, including nitrogen fixation and plant
growth regulation (Ahmad et al., 2008). Around the last two decades, the use of plant
growth-promoting microorganisms for sustainable agriculture has increased
substantially worldwide. Scientists have found considerable improvements in the
growth and production of main agronomic crops due to them (Amara & Dahdoh, 1995;
Hilali et al., 2001; Asghar et al., 2002). Microbes aid in converting phosphorus from an
insoluble state to a plant-available form (Istina et al., 2015). The ability of plant-growth
promoting bacteria (PGPB) strongly supports to control drought stress in wheat (Kasim
et al., 2013). According to Verma et al., (2013) microbes boost nodulation, nutrient
absorption, and chickpea yield. Furthermore, it is noticed that many bacterial species
have been used for the mineralization of organic contaminants in soil, i.e.
bioremediation of soil pollutants (Zaidi et al., 2006). Three types of machines are
typically studied and put forward to describe that how microscopic activity can lead to
the improvement in plant growth: (a) by manipulating the plant hormonal signalling
(Verbon & Liberman, 2016), (b) by pathogenic microscopic strain outcompeting or
repelling (Mendes et al., 2013), (c) by increasing soil-borne nutrients bioavailability
(Van Der Heijden et al., 2008).
Bacteria like rhizospheric and the endophytic contributes to promoting plant
growth by producing several hormones, inhibiting the pathogens and enhancing the
availability of nutrients (Friesen et al., 2011). Some microorganism that is beneficial for
plants growth are free-living bacteria (Azotobacter), association forming bacteria with
plants (Azospirillum) and bacteria that for symbiosis relationship with legumes and
other crop plants (Rhizobium and Bradyrhizobium) (Postgate, 1982). In addition, soil
microorganisms help improve soil productivity and the overall ecosystem, so these are
being used worldwide to enhance the growth and yield of plants.
Microorganisms require adenosine triphosphate (ATP) 16 molecules during
nitrogen fixation to reduce each mole of nitrogen. Microorganisms obtain this energy by
the oxidation of organic molecules. In the case of free-living organisms, energy is
obtained by other organisms. Photosynthetic organisms like cyanobacteria utilize sugars
that is an end product of photosynthesis. Associative or symbiotic N-fixing
microorganisms get such compounds from the rhizosphere of host plants (Hubbell &
Kidder, 1998), such as:

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1) Increase in root growth either by root systems extensions like mycorrhizal


associations or stimulation of hormones for root growth, root branching,
development of root hair or phytostimulation; like indole-3-acetic acid production,
involving of enzymes that change ethylene precursors in plants, like 1-
aminocyclopropane-1-carboxylate deaminase (Hayat et al., 2010).
2) Change in sorption equilibria. This has resulted in a higher net transfer of ions like
orthophosphate into the soil solution. Facilitation of organic phosphorus mobility
directly or indirectly by microbial turnover (Seeling & Zasoski, 1993).
3) Metabolic processes induction. these are effective in phosphorus solubilizing and
mineralizing directly from the sparingly available soil inorganic and organic
phosphorus forms (Richardson et al., 2009).

Role of Microbes in Food Industries


Microbes play a crucial part in the food industry. They are involved in distributing
a wide range of foods, and they are often responsible for food poisoning, which leads to
inebriation and illness (Koch, 1990). Microbial contamination of food happens most
often during the shipment of foods from the field to the distribution plant and during
processing, packaging, transportation, collection, and before use. While most
pathogenic or disease-causing bacteria are gramme negative (-ve), food poisoning has
been compared to three (3) gramme positive (+ve) rods: Botulinum toxin is a toxin
produced by Clostridium botulinum (Srivastava, 2003). They expand quickly and take
up less space than traditional crops. Petroleum oil, methanol, ethanol, sugar, molasses,
and waste from paper mills are among the low-cost agricultural and industrial waste
materials they eat. The secondary advantage is that they help with product recycling,
which reduces emissions. They grow a large quantity of fruit. In a 1000 pound yeast
growth medium, several tonnes of protein is produced in a single day. This is about 10-
15 times more than soybeans and 25-50 times more than maize (Hardy, 2002). This is
because the cells have a lot of protein. In yeast cells, protein content ranges from 40 to
50 percent; in algae, protein content ranges from 20 to 40 percent.

Role of Microbes in Food Preservation


Nature uses microorganisms to carry out fermentation processes, and yeasts,
moulds, and bacteria have been used to produce bread, vinegar, beer, yoghurt, cheese,
and wine and fruit, vegetables, and fermented fish for thousands of years. Fermentation
is the earliest methods of food transformation and preservation. This biological process
increases the nutritional and organoleptic properties of food while still preserving it.
Microbes are used in the fermentation of dairy products and the manufacture of
alcoholic beverages. Sour cream, yoghurt, buttermilk, curd, bread, and cheese are some
of the most popular dairy products processed by microbes. Soil organisms are also
members of many food chains, acting as sources of nutrients for one another, and are
often the prominent members of soil biota food chains.

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Use of Microbes in Food Production and Preservation


Microorganisms are used by nature for the fermentation process. Humanity uses
microorganism from thousands of years, including yeasts, moulds, and bacteria, to
produce food products like bread, beer, wine, vinegar, yoghurt, cheese, fermented fish,
meat, and vegetables. Microbial cultures are used as “meat starter” cultures to produce
dried and fermented products, i.e. salami, pepperoni, chorizo and dried ham. Bacteria
like lactic bacteria are used in the flavour and colour development of different products.
Lactic acid bacteria also play a vital role in converting unstable malic acid (naturally
present in the wine) into a stable form. This conversion has resulted in high-quality
wine product, and for wine storage, these are beneficial. Some other bacterial strain like
Streptococcus, Lactobacillus, Bifidobacterium and Erwinia is used in fermented food
and dairy products production. Yoghurt is produced by the presence of Streptococcus
thermophilus and Lactobacillus bulgaricus.
Moulds are being used in different food manufacturing, i.e. cheese. For ripening
of sausages surface, preservation of products natural qualities and flavours
development, moulds are commonly used. Molds are being grown as a source of feed
and food too. These are also employed for ingredients production like enzymes, i.e.
amylase used in bread making or citric acid for soft drinks. These are considered
significant contributors to the ripening of several oriental foods. Like Bothrytiscinerea
spp. is involved in grape rotting and wine production. In lactic fermentations, moulds
are used that resulted in unique fermented milk known as “viili”. Yeasts are involved in
the fermentation process like in wine; it produces alcohol.

Post-harvest Damages of Microbes


When the crops are harvested, it undergoes series of different operations that can
cause quantitative and qualitative losses. When the grain and pulses are not thoroughly
dried, the chances of mould development by microorganism are higher. Approximately
all agricultural products stored over a long time period are widely subjected to a various
microorganism that causes food products degradation by rotting or reducing palatability
of products by inducing odd taste. Some organisms, i.e. Aspergillus, Penicillium, and
Fusarium, can produce mycotoxins on food, making the food unsafe, which is the
primary cause of disease and even death in humans as well in animals (Law & Cooper,
2001). Yeasts that is responsible for food spoilage is divided into four groups:
1) Zygosaccharomyces cause spoilage in honey, dried fruit, jams, and soy sauce. It can
tolerate higher concentrations of sugar and salts (Rawat, 2015).
2) Debaryomyces hansenii can grow at greater concentrations of salts (up to 24%).
Therefore, cured meats, cheeses, and olives in salt brine are spoiled by this one
(Mandrell et al., 2005).
3) Some Saccharomyces spp. strains can spoil alcoholic beverages products, i.e. wine
spoilage, by causing turbidity, gassiness, and off-flavours in it. However, some
species of the same group can grow on the fruits, even on the yoghurt containing
fruits, which are heat resistant (Elez-Martinez et al., 2004).

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4) Fermented foods are mainly spoiled by Dekkera/Brettanomyces, i.e. alcoholic


beverages and dairy products, including milk. Because they can produce volatile
phenolic compounds that cause off-odour and make the flavour unpleasant, it
ultimately resulted in massive loss to the winery business (Couto et al., 2005).

In Fruits and Vegetables


As fruits and vegetables are perishable and contain higher contents of moisture.
So these are highly susceptible to spoilage caused by microbes like fungi, bacteria, yeast
and moulds. So during their post-harvest period, fungal and bacterial diseases can cause
considerable loss (Yahaya & Mardiyya, 2019; Ch et al., 2021). These pathogens can
cause the rotting of items and make them unfit for consumption as they produce
mycotoxin (Fatima et al., 2009). Common pathogens involved in post-harvest damage
are Alternaria, Fusarium, Penicillium, Aspergillus, Geotrichum, Phytophthora and
Botrytis; these are responsible for about 10-30% yield reduction in a vegetable like a
tomato (Etebu et al., 2013). Penicillium expansum is the significant and essential
pathogen during a post-harvest period of pear fruit in storage that can cause the blue
mould rot. This leads to substantial economic losses during the postharvest period
(Amin et al., 2017; Haroon et al., 2020).

In Flowers
In flowers, the damage is also caused by microorganisms after harvesting,
primarily due to interruption of water contents because microorganisms start their
growth in the vas solution, so the xylem blockage is faced in the stored flowers stem.
Blockage of the branch is mainly caused by a microorganism and some other factors
like by production of extracellular polysaccharides and dead cells degradation. Bacteria
are also involved in the secretion of pectinases, some toxic compounds and ethylene,
accelerating the senescence process. It was also observed that besides reduction in vase
life, water relations disruption in the flowers (rose) could cause a problem in flower
opening, the flowers are opened improperly, or maybe flowers start to wilt (Jowkar et
al., 2012).

In Dairy Products
Bacteria that cause milk spoilage may be originated on-farm environment or the
milking equipment or in the processing plants, transferred from the kit, employees or
maybe from the air. When the lactic acid and some other compounds are produced, off-
flavour is milk is observed, especially when the microorganism’s population reach 106
CFU/ml. Refrigeration is helpful in lactic acid bacteria growth suppression. Still, other
psychrophilic bacteria, i.e. Pseudomonas, Alcaligenes, Enterobacter, and some spore-
formers, can grow within 25 hours and produce rancid odours (Dogan & Boor, 2003).
The process of pasteurization can kill both psychrophiles and mesophilic bacteria (lactic
acid bacteria). However, heat-tolerant species like Alcaligenes, Microbacterium,

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Bacillus and Clostridium can survive and, in the end, cause milk spoilage and in other
dairy products (Rawat, 2015).

In Eggs
Under poor conditions of storage and management, the percentage of moisture in
eggshell is increased, making it favourable for the growth of fungal (mould) that cause
eggshells spoilage. When the fungal activities are grown on the surface of eggshells, the
pore size on the eggshell is increased, and the microbe’s entry through the shell
becomes easier. Types of spoilage can be characterized by egg colour very often
(Erkmen & Bozoglu, 2016).

Precautions
Nature uses microorganisms to carry out fermentation processes, and yeasts,
moulds, and bacteria have been used to produce bread, vinegar, beer, yoghurt, cheese,
and wine and fruit, vegetables, and fermented fish for thousands of years. Fermentation
is the earliest methods of food transformation and preservation. This biological process
increases the nutritional and organoleptic properties of food while still preserving it.
Sour cream, yoghurt, buttermilk, curd, bread, and cheese are some of the most popular
dairy products processed by microbes.
Bacteria, moulds, and yeast are the most common microorganisms that cause food
spoilage and are widely used in food and food product processing. For the fermentation
of dairy products, various bacteria and fungi are used to produce a broad range of
cultured milk products. In these cheese-making techniques, bacteria and fungi are also
included. Lactic acid bacteria coagulate milk, which can then be used to produce
various cheeses such as soft unripened, soft-ripened, semisoft, rugged, and very hard
types. Microbes such as Bifidobacterium and Lactobacillus are used in the food
industry. For example, alcoholic drinks and other beer products are made by fermenting
cereals and grains with various yeast strains.
We can prevent pathogenic infections by volatile organic compounds. Bio-
fumigation is a gaseous treatment; this can be used to get rid of this problem. Different
plants that produce volatiles can be used as potential substances for biofumigation
(Stelmach-Mardas et al., 2016). To prevent microbial growth in the vase solutions of cut
flowers, several compound/chemical like silver nitrate, silver thiosulphate, aluminium
sulfate, and sodium hypochlorite are considered beneficial (Liao et al., 2000).
Organisms like yeasts, i.e. Pichia guilliermondii, are used to control post-harvest
damages like rot in citrus and some other fruits. Like for the post-harvest apple, rot
prevention species of Cryptococcus and Acremonium breve are helpful. These are also
used to control rot in pear (Schnable et al., 2009). To preserve food and beverages, safe
additives that are anti-microbial are added, like acetic, sorbic, and benzoic acids
(Choudhary et al., 2017). Some other commonly used preservation agents are following:
acetic, lactic, benzoic, and sorbic acid. These are capable of inhibiting fungal and

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bacterial cells outgrowth. Specially sorbic acid is utilized to inhibit bacterial spore’s
germination and outgrowth (Rawat, 2015).
Crops grains are preserved for a long time after their post-harvest, so these are
treated with some pesticides, drying, debranding, irradiation, and other ozone treatment.
Chlorine and hypochlorite are also helpful for this purpose (Los & Timmer, 2018). milk
spoilage is controlled by the process of pasteurization or sterilization and milk
dehydration. For dairy products, preservation of antimicrobial agents and some
generally regarded as safe (GRAS) substances have used that work as food additives,
stabilizers and emulsifiers (Verma et al., 2017). Eggs spoilage can be avoided if the
conditions are maintained during storage. By washing or applying disinfectants
(hypochlorite solution), microbes can be removed from eggs. Eggs pasteurization or use
higher or lower temperature treatments would conceal microbially (Erkmen & Bozoglu,
2016).

Future Opportunites
Among the most creative technologies, research and industry are increasingly
focused on the synthesis of microbial protein (MP) for regulated and intense systems
known as "bioreactors." MP has modest needs for arable land, does not compete directly
with crop-based food products and uses fertilizer at an efficiency of about 100%.
Furthermore, from an economic viewpoint, currently only in specific market segments,
such as aquaculture, use of MP in animal feeding is justified. Finally, the European
Food Safety Authority must approve innovative MPs before accessing the market.
Because of the externalised environmental problems of the existing agri-food
production system, the use of microorganisms as food will depend primarily on
favourable laws, public acceptability, and lower prices.

CONCLUSION
Microbes perform better functions for agricultural production by promoting
various direct and indirect mechanisms in soil and plants. Bacteria are the best plentiful
microbes in the ground, followed by fungi, actinomycetes, soil protozoa and soil algae.
A single gramme of soil can contain hundreds of millions to billions of microbes.
Therefore, if agricultural development is to satisfy the needs of an increasing global
population, a deeper understanding of soil microbiology is needed. Unfortunately,
agricultural activities continue to endanger the stable microbe population in many areas
rather than encourage the stable microbe population.
Furthermore, food decay and spoilage are caused by microbes. A rotten food
product has a foul odour, flavour and texture. Despite their pathogenicity, microbes play
a substantial role in dispensing fermented drinks and foods in the food industry and
home. Probiotics, fermented foods and alcoholic beverages are flattering extra popular
due to their health benefits and flavour. Microbes influence agricultural productivity
through a variety of overt and indirect processes in soil and plants. Bacteria, fungi,
actinomycetes, plant protozoa, and soil algae are the most abundant bacteria in the

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ground. Bacteria, moulds, and yeast are the most common microorganisms that cause
food spoilage and are widely used in food and food product processing. A single
gramme of soil can contain hundreds of millions to billions of microbes and thus; can be
used for enhancing yield and growth directly and indirectly.

REFERENCES
Ahmad, F., Ahmad, I., & Khan, M. S. (2008). Screening of free-living rhizospheric
bacteria for their multiple plant growth promoting activities. Microbiological
Research, 163(2), 173-181. https://doi.org/10.1016/j.micres.2006.04.001.
Amara, M. A., & Dahdoh, M. S. A. (1995). Effect of inoculation with plant-growth
promoting rhizobacteria, Pgpr on yield and uptake of nutrients by wheat grown on
sandy soil. In 5. National Congress on Bio-Agriculture in Relation to
Environment, Cairo (Egypt), 20-21 November 1995.
Amin, S. A., Ahmed, A. I., Sidiq, S. A., & Mahmood, H. A. (2017). Biological control
of post-harvest disease of blue mould (Penicillium expansum) of pear fruit by
using antagonist microorganisms under laboratory and cold storage conditions.
International Journal of Current Research in Biosciences and Plant Biology, 4(1),
20-31. http://dx.doi.org/10.20546/ijcrbp.2017.401.004.
Asghar, H., Zahir, Z., Arshad, M., & Khaliq, A. (2002). Relationship between in vitro
production of auxins by rhizobacteria and their growth-promoting activities in
Brassica juncea L. Biology and Fertility of Soils, 35(4), 231-237.
https://doi.org/10.1007/s00374-002-0462-8.
Berg, M. P., Kiers, E. T., Driessen, G., Van Der Heijden, M., Kooi, B. W., Kuenen, F.,
Liefting, M., Verhoef, H. A., & Ellers, J. (2010). Adapt or disperse: understanding
species persistence in a changing world. Global Change Biology, 16(2), 587-598.
https://doi.org/10.1111/j.1365-2486.2009.02014.x.
Bossio, D. A., Scow, K. M., Gunapala, N., & Graham, K. J. (1998). Determinants of
soil microbial communities: effects of agricultural management, season, and soil
type on phospholipid fatty acid profiles. Microbial ecology, 36(1), 1-12.
https://doi.org/10.1007/s002489900087.
Brown, G. G., Barois, I., & Lavelle, P. (2000). Regulation of soil organic matter
dynamics and microbial activityin the drilosphere and the role of interactionswith
other edaphic functional domains. European Journal of Soil Biology, 36(3-4),
177-198. https://doi.org/10.1016/S1164-5563(00)01062-1.
Caplice, E., & Fitzgerald, G. F. (1999). Food fermentations: role of microorganisms in
food production and preservation. International Journal of Food Microbiology,
50(1-2), 131-149. https://doi.org/10.1016/S0168-1605(99)00082-3.
Ch, B. I., Gondal, A. H., Zafar, H., Umar, H., Toor, M. D., Zafar, A., Farooq, Q., &
Saeed, S. (2021). A brief correspondence on glyphosate remediation using
microbes and mineral sources. Annals of Reviews and Research, 6(4), 555693.
http://dx.doi.org/10.19080/ARR.2021.06.555693.
Choudhary, M., Kumar, R., Datta, A., Nehra, V., & Garg, N. (2017). Bioremediation of
heavy metals by microbes. In Bioremediation of salt affected soils: an Indian
perspective (pp. 233-255). Springer, Cham. https://doi.org/10.1007/978-3-319-
48257-6_12.
Colica, G., Li, H., Rossi, F., Li, D., Liu, Y., & De Philippis, R. (2014). Microbial
secreted exopolysaccharides affect the hydrological behavior of induced

Agrinula : Jurnal Agroteknologi dan Perkebunan, 2021 vol. 4 (2): 106-121


117

biological soil crusts in desert sandy soils. Soil Biology and Biochemistry, 68, 62-
70. https://doi.org/10.1016/j.soilbio.2013.09.017.
Compant, S., Clément, C., & Sessitsch, A. (2010). Plant growth-promoting bacteria in
the rhizo-and endosphere of plants: their role, colonization, mechanisms involved
and prospects for utilization. Soil Biology and Biochemistry, 42(5), 669-678.
https://doi.org/10.1016/j.soilbio.2009.11.024.
Couto, J. A., Neves, F., Campos, F., & Hogg, T. (2005). Thermal inactivation of the
wine spoilage yeasts Dekkera/Brettanomyces. International Journal of Food
Microbiology, 104(3), 337-344.
https://doi.org/10.1016/j.ijfoodmicro.2005.03.014.
Dogan, B., & Boor, K. J. (2003). Genetic diversity and spoilage potentials among
Pseudomonas spp. isolated from fluid milk products and dairy processing plants.
Applied and Environmental Microbiology, 69(1), 130-138.
https://doi.org/10.1128/AEM.69.1.130-138.2003.
Egamberdiyeva, D. (2007). The effect of plant growth promoting bacteria on growth
and nutrient uptake of maize in two different soils. Applied Soil Ecology, 36(2-3),
184-189. https://doi.org/10.1016/j.apsoil.2007.02.005.
Egli, M., Wernli, M., Burga, C., Kneisel, C., Mavris, C., Valboa, G., Mirabella, A.,
Plötze, M., & Haeberli, W. (2011). Fast but spatially scattered smectite-formation
in the proglacial area Morteratsch: An evaluation using GIS. Geoderma, 164(1-2),
11-21. https://doi.org/10.1016/j.geoderma.2011.05.001.
Elez-Martinez, P., Escola-Hernandez, J., Soliva-Fortuny, R. C., & Martin-Belloso, O.
(2004). Inactivation of Saccharomyces cerevisiae suspended in orange juice using
high-intensity pulsed electric fields. Journal of Food Protection, 67(11), 2596-
2602. https://doi.org/10.4315/0362-028X-67.11.2596.
Erkmen, O., & Bozoglu, T. F. (2016). Food Microbiology, principles into practice. John
Wiley & Sons.
Etebu, E., Nwauzoma, A. B., & Bawo, D. D. S. (2013). Postharvest spoilage of tomato
(Lycopersicon esculentum Mill.) and control strategies in Nigeria. Journal of
Biology, Agriculture and Healthcare, 3(10), 51-61.
FAO. (2017). Voluntary guidelines for sustainable soil management. Food and
Agriculture Organization of the United Nations, Rome, Italy. Available online:
http://www.fao.org/documents/card/en/c/5544358d-f11f-4e9f-90ef-
a37c3bf52db7/.
Fatima, N., Batool, H., Sultana, V., Ara, J., & Ehteshamul-Haque, S. (2009). Prevalence
of post-harvest rot of vegetables and fruits in Karachi, Pakistan. Pakistan Journal
of Botany, 41(6), 3185-3190.
Friesen, M. L., Porter, S. S., Stark, S. C., von Wettberg, E. J., Sachs, J. L., & Martinez-
Romero, E. (2011). Microbially mediated plant functional traits. Annual Review of
Ecology, Evolution, and Systematics, 42, 23-46. https://doi.org/10.1146/annurev-
ecolsys-102710-145039.
Gondal, A. H., Zafar, A., Zainab, D., Toor, M. D., Sohail, S., Ameen, S., Ijaz, A. B., Ch,
B. I., Hussain, I., Haider, S., Ahmad, I. A., Rehman, B., & Younas, N. (2021a). A
detailed review study of zinc involvement in animal, plant and human nutrition.
Indian Journal of Pure & Applied Biosciences, 9(2), 262-271.
Gondal, A. H., Hussain, I., Ijaz, A. B., Zafar, A., Ch, B. I., Zafar, H., Sohail, M. D.,
Khan, A. A., Niazi, H., Yousaf, H., Usama, M., Touseef, M., & Tariq, M.
(2021b). Influence of soil pH and microbes on mineral solubility and plant

Agrinula : Jurnal Agroteknologi dan Perkebunan, 2021 vol. 4 (2): 106-121


118

nutrition: A review. International Journal of Agriculture and Biological Sciences,


5(1), 71-81. http://dx.doi.org/10.5281/zenodo.4625364.
Gondal, A. H., Zafar, A., Toor, M. D., Ijaz, A. B., Sohail, S., Ch, B. I., Nawaz, T.,
Haider, S., Ahmad, I. A., Dua-e-Zainab., Rehman, B., Qasim, M., Shah, M. S., &
Rehman, M. U. (2021c). Alleviation of zinc deficiency from humans through
plants by organic sources: A powerful tonic. International Journal of Applied
Research, 7(4), 240-243.
Gondal, A. H., Zafar, A., Zainab, D., Toor, M. D., Sohail, S., Ameen, S., Ijaz, A. B., Ch,
B. I., Hussain, I., Haider, S., Ahmad, I. A., Rehman, B., & Younas, N. (2021d).
Adaptability of soil pH through innovative microbial approach. Current Research
in Agricultural Sciences, 8(2), 71-79.
Graham, P. H., & Vance, C. P. (2003). Legumes: importance and constraints to greater
use. Plant Physiology, 131(3), 872-877. https://doi.org/10.1104/pp.017004.
Hardy, S. (2002). Human microbiology. Taylor & Francis, London.
Haroon, A., Yaseen, T., Husnain, A., Mehfooz, M., Jarar, M. H., Naveed, U., & Khalid,
F. (2020). Water resources helps in the expansion of mosquitoes colonies. Big
Data In Water Resources Engineering (BDWRE), 1(1), 16-21.
http://dx.doi.org/10.26480/bdwre.01.2020.16.21.
Hayat, R., Ali, S., Amara, U., Khalid, R., & Ahmed, I. (2010). Soil beneficial bacteria
and their role in plant growth promotion: a review. Annals of Microbiology, 60(4),
579-598. https://doi.org/10.1007/s13213-010-0117-1.
Hilali, A., Prévost, D., Broughton, W. J., & Antoun, H. (2001). Effets de l'inoculation
avec des souches de Rhizobium leguminosarum biovar trifolii sur la croissance du
blé dans deux sols du Maroc. Canadian Journal of Microbiology, 47(6), 590-593.
https://doi.org/10.1139/w01-068.
Hubbell, D. H., & Kidder, G. (1998). Biological nitrogen fixation. University of Florida
Cooperative Extension Service, Institute of Food and Agriculture Sciences, EDIS.
Istina, I. N., Widiastuti, H., Joy, B., & Antralina, M. (2015). Phosphate-solubilizing
microbe from Saprists peat soil and their potency to enhance oil palm growth and
P uptake. Procedia Food Science, 3, 426-435.
https://doi.org/10.1016/j.profoo.2015.01.047.
Jowkar, M. M., Kafi, M., Khalighi, A., & Hasanzadeh, N. (2012). Postharvest
physiological and microbial impact of hydroxy quinoline citrate as ‘Cherry
Brandy’rose vase solution biocide. Annals of Biological Research, 3(5), 2238-
2247.
Kasim, W. A., Osman, M. E., Omar, M. N., Abd El-Daim, I. A., Bejai, S., & Meijer, J.
(2013). Control of drought stress in wheat using plant-growth-promoting bacteria.
Journal of Plant Growth Regulation, 32(1), 122-130.
https://doi.org/10.1007/s00344-012-9283-7.
Koch, A. L. (1990). Growth and form of the bacterial cell wall. American Scientist,
78(4), 327-341. https://www.jstor.org/stable/29774119?seq=1.
Kumar, A., & Verma, J. P. (2019). The role of microbes to improve crop productivity
and soil health. In Ecological Wisdom Inspired Restoration Engineering (pp. 249-
265). Springer, Singapore. https://doi.org/10.1007/978-981-13-0149-0_14.
Law, S. E., & Cooper, S. C. (2001). Air-assisted electrostatic sprays for postharvest
control of fruit and vegetable spoilage microorganisms. IEEE Transactions on
Industry Applications, 37(6), 1597-1602. https://doi.org/10.1109/28.968166.

Agrinula : Jurnal Agroteknologi dan Perkebunan, 2021 vol. 4 (2): 106-121


119

Liao, W., Ho, C. Y., Yan, Y. L., Postlethwait, J., & Stainier, D. Y. (2000). Hhex and scl
function in parallel to regulate early endothelial and blood differentiation in
zebrafish. Development, 127(20), 4303-4313.
https://doi.org/10.1242/dev.127.20.4303.
Lombard, N., Prestat, E., van Elsas, J. D., & Simonet, P. (2011). Soil-specific
limitations for access and analysis of soil microbial communities by
metagenomics. FEMS Microbiology Ecology, 78(1), 31-49.
https://doi.org/10.1111/j.1574-6941.2011.01140.x.
Los, B., & Timmer, M. P. (2018). Measuring bilateral exports of value added: a unified
framework (No. w24896). National Bureau of Economic Research.
Mandrell, R. E., Gorski, L., & Brandl, M. T. (2005). Attachment of microorganisms to
fresh produce. In Microbiology of fruits and vegetables (pp. 49-90). CRC Press.
Mendes, R., Garbeva, P., & Raaijmakers, J. M. (2013). The rhizosphere microbiome:
significance of plant beneficial, plant pathogenic, and human pathogenic
microorganisms. FEMS Microbiology Reviews, 37(5), 634-663.
https://doi.org/10.1111/1574-6976.12028.
Mendes, R., Kruijt, M., de Bruijn, I., Dekkers, E., van der Voort, M., Schneider, J. H.,
Piceno, Y., DeSantis, T., Andersen, G., Bakker, P, A, H, M., & Raaijmakers, J. M.
(2011). Deciphering the rhizosphere microbiome for disease-suppressive bacteria.
Science, 332(6033), 1097-1100. https://doi.org/10.1126/science.1203980.
Miransari, M. (2011a). Soil microbes and plant fertilization. Applied Microbiology and
Biotechnology, 92(5), 875-885. https://doi.org/10.1007/s00253-011-3521-y.
Miransari, M. (2011b). Interactions between arbuscular mycorrhizal fungi and soil
bacteria. Applied Microbiology and Biotechnology, 89(4), 917-930.
https://doi.org/10.1007/s00253-010-3004-6.
Postgate, J. R. (1982). The fundamentals of nitrogen fixation. New York: Cambridge
University Press.
Qiao, Q., Wang, F., Zhang, J., Chen, Y., Zhang, C., Liu, G., Zhang, H., Ma, C., &
Zhang, J. (2017). The variation in the rhizosphere microbiome of cotton with soil
type, genotype and developmental stage. Scientific Reports, 7, 3940.
https://doi.org/10.1038/s41598-017-04213-7.
Rakhmanova, A., Khan, Z. A., & Shah, K. (2018). A mini review fermentation and
preservation: role of lactic acid bacteria. MOJ Food Processing & Technology,
6(5), 414-417.
Rashid, M. I., Mujawar, L. H., Shahzad, T., Almeelbi, T., Ismail, I. M., & Oves, M.
(2016). Bacteria and fungi can contribute to nutrients bioavailability and
aggregate formation in degraded soils. Microbiological Research, 183, 26-41.
https://doi.org/10.1016/j.micres.2015.11.007.
Rawat, S. (2015). Food Spoilage: Microorganisms and their prevention. Asian Journal
of Plant Science and Research, 5(4), 47-56.
Richardson, A. E., Barea, J. M., McNeill, A. M., & Prigent-Combaret, C. (2009).
Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth
promotion by microorganisms. Plant and Soil, 321(1), 305-339.
https://doi.org/10.1007/s11104-009-9895-2.
Rossi, F., & De Philippis, R. (2015). Role of cyanobacterial exopolysaccharides in
phototrophic biofilms and in complex microbial mats. Life, 5(2), 1218-1238.
https://doi.org/10.3390/life5021218.

Agrinula : Jurnal Agroteknologi dan Perkebunan, 2021 vol. 4 (2): 106-121


120

Rossi, F., Olguín, E. J., Diels, L., & De Philippis, R. (2015). Microbial fixation of CO 2
in water bodies and in drylands to combat climate change, soil loss and
desertification. New Biotechnology, 32(1), 109-120.
https://doi.org/10.1016/j.nbt.2013.12.002.
Schnable, P. S., Ware, D., Fulton, R. S., Stein, J. C., Wei, F., Pasternak, S., et al.,
(2009). The B73 maize genome: complexity, diversity, and dynamics. Science,
326(5956), 1112-1115. https://doi.org/10.1126/science.1178534.
Seeling, B., & Zasoski, R. J. (1993). Microbial effects in maintaining organic and
inorganic solution phosphorus concentrations in a grassland topsoil. Plant and
Soil, 148(2), 277-284. https://doi.org/10.1007/BF00012865.
Semhi, K., Chaudhuri, S., Clauer, N., & Boeglin, J. L. (2008). Impact of termite activity
on soil environment: A perspective from their soluble chemical components.
International Journal of Environmental Science & Technology, 5(4), 431-444.
https://doi.org/10.1007/BF03326039.
Shameer, S., & Prasad, T. N. V. K. V. (2018). Plant growth promoting rhizobacteria for
sustainable agricultural practices with special reference to biotic and abiotic
stresses. Plant Growth Regulation, 84(3), 603-615.
https://doi.org/10.1007/s10725-017-0365-1.
Sofo, A., Mininni, A. N., & Ricciuti, P. (2020). Soil macrofauna: A key factor for
increasing soil fertility and promoting sustainable soil use in fruit orchard
agrosystems. Agronomy, 10(4), 456. https://doi.org/10.3390/agronomy10040456.
Srivastava, S. (2003). Understanding bacteria. Springer Science & Business Media.
Stelmach-Mardas, M., Rodacki, T., Dobrowolska-Iwanek, J., Brzozowska, A.,
Walkowiak, J., Wojtanowska-Krosniak, A., Zagrodzki, P., Bechthold, A., Mardas,
M., & Boeing, H. (2016). Link between food energy density and body weight
changes in obese adults. Nutrients, 8(4), 229.
http://dx.doi.org/10.3390/nu8040229.
Van Der Heijden, M. G. A., Bardgett, R. D., & Van Straalen, N. M. (2008). The unseen
majority: soil microbes as drivers of plant diversity and productivity in terrestrial
ecosystems. Ecology Letters, 11(3), 296-310. https://doi.org/10.1111/j.1461-
0248.2007.01139.x.
Verbon, E. H., & Liberman, L. M. (2016). Beneficial microbes affect endogenous
mechanisms controlling root development. Trends in Plant Science, 21(3), 218-
229. https://doi.org/10.1016/j.tplants.2016.01.013.
Verma, J. P., Yadav, J., Tiwari, K. N., & Kumar, A. (2013). Effect of indigenous
Mesorhizobium spp. and plant growth promoting rhizobacteria on yields and
nutrients uptake of chickpea (Cicer arietinum L.) under sustainable agriculture.
Ecological Engineering, 51, 282-286.
https://doi.org/10.1016/j.ecoleng.2012.12.022.
Verma, S., Marshall, J., Gerbig, C., Rödenbeck, C., & Totsche, K. U. (2017). The
constraint of CO2 measurements made onboard passenger aircraft on surface–
atmosphere fluxes: the impact of transport model errors in vertical mixing.
Atmospheric Chemistry and Physics, 17(9), 5665-5675.
https://doi.org/10.5194/acp-17-5665-2017.
Veum, K. S., Goyne, K. W., Kremer, R. J., Miles, R. J., & Sudduth, K. A. (2014).
Biological indicators of soil quality and soil organic matter characteristics in an
agricultural management continuum. Biogeochemistry, 117(1), 81-99.
https://doi.org/10.1007/s10533-013-9868-7.

Agrinula : Jurnal Agroteknologi dan Perkebunan, 2021 vol. 4 (2): 106-121


121

Wani, F. S., Ahmad, L., Ali, T., & Mushtaq, A. (2015). Role of microorganisms in
nutrient mobilization and soil health - a review. Journal of Pure and Applied
Microbiology, 9(2), 1401-1410.
Yahaya, S. M., & Mardiyya, A. Y. (2019). Review of post-harvest losses of fruits and
vegetables. Biomedical Journal of Scientific & Technical Research, 13(4), 10192-
10200. http://dx.doi.org/10.26717/BJSTR.2019.13.002448.
Zaidi, S., Usmani, S., Singh, B. R., & Musarrat, J. (2006). Significance of Bacillus
subtilis strain SJ-101 as a bioinoculant for concurrent plant growth promotion and
nickel accumulation in Brassica juncea. Chemosphere, 64(6), 991-997.
https://doi.org/10.1016/j.chemosphere.2005.12.057.
Zhu, Y. G., Zhao, Y., Zhu, D., Gillings, M., Penuelas, J., Ok, Y. S., Capon, A., &
Banwart, S. (2019). Soil biota, antimicrobial resistance and planetary health.
Environment International, 131, 105059.
https://doi.org/10.1016/j.envint.2019.105059.

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