Role of Microbes in Plant Growth and Food Preservation: AGRINULA Jurnal Agroteknologi Dan Perkebunan June 2021
Role of Microbes in Plant Growth and Food Preservation: AGRINULA Jurnal Agroteknologi Dan Perkebunan June 2021
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Review Article
website : https://journal.utnd.ac.id/index.php/agri
E-ISSN : 2655-7673
DOI : https://doi.org/10.36490/agri.v4i2.158
1
Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad
38040, Pakistan.
2
European University of Lefke, Institute of Graduate Studies and Research, Department
of Environmental Sciences Northern Cyprus TR-10 Mersin Turkey.
*Corresponding author: aqarabhusnain944@gmail.com
INTRODUCTION
The soil ecosystem is comprised of inorganic material (iron calcium, oxides,
sulfur, nitrates, phosphates, stone particles, and ash), substrates (dead organisms, fallen
leaves, dead roots, and rotten wood), organisms (microorganisms, plants, and animals),
water and air. It also provides food, fibre, fruit and shelter to all living organisms. The
use of agrochemicals in industries and agricultural production leads to contamination of
the environment that is a severe problem in the coming days. Furthermore, the world
population is increasing by leaps and bounds; thus, already used anthropogenic
activities are not sufficient to produce maximum food. Again, agricultural products that
are used in the industry become tasteless and poison due to microbial degradation.
Microbes play an essential role in plant growth and development in a variety of
pathway. Plant growth-promoting organisms are used to boost agricultural crop growth
and yields under natural and stressful conditions. It enhances the growth of crop plants
by improving a variety of physiological parameters in response to external stimulants
through various mechanisms. Plant growth regulators, the improvement of several
metabolites, and the conversion of atmospheric (O2) nitrogen into ammonia (NH4+) are
some of the mechanisms that can take part in the growth elevation of plants. It also
provides resistance from pathogens through induced systemic resistance and systemic
acquired resistance (Kumar & Verma, 2019).
Microorganisms are well known for their involvement in food spoilage. Still, they
also play a beneficial role in the food industry as they are helpful in the preservation of
food and dairy products, i.e. fermentation. In the process of fermentation,
microorganism are used, and they increase the shelf life of food. Lactic acid bacteria
(LAB) are used in several fermentation and preservation process as their metabolic
characteristics are unique. For example, milk, meats, cereals, and vegetables (Caplice &
Fitzgerald, 1999). The unique ability of LAB is a production of Bacteriocins. It is an
anti-microbial compound, so LAB is used as a preservation agent. In milk fermentation,
it is used as a “starter culture”, so it produces acid in the fermentation process of milk
(Rakhmanova et al., 2018). Microorganisms play a vital role in the growth of plants.
They live in the rhizosphere of plants, and it is an interface of plant-microbe where they
interact with the plants' roots, and many processes like exchange of materials and
signalling process occur (Qiao et al., 2017). There is a strong interaction between plants
and microorganisms living in the soil. They are involved in many essential mechanisms
for both communities of plants and microbes, e.g. neutral, synergistic or antagonistic
mechanisms. Among microorganisms living in the rhizosphere, plant growth-promoting
bacteria (PGPB) are most important because they stimulate the growth of plants by
several processes, i.e. nitrogen fixation, regulation of soil nutrients availability,
suppression of pathogens, and modify the response of plants for biotic and abiotic
stresses (Shameer & Prasad, 2018).
Hence the study of microbes towards agricultural production and food
preservation in the industry is essential. Previous studies show that microbes play a
crucial role in almost every field of life. The main objectives of this study are (a) to
highlight the role of microbes in plant growth and development, (b) to demonstrate the
role of microbes in food industries.
RESULT REVIEW
Role of Microbes in Soil Development
The presence of microorganisms influences the formation and development of
soils. During soil formation, microorganisms produce several pores and crevices so that
roots of plants can easily penetrate the cracks and production of soil fragmentation is
increased. Many factors control the rate of soil development, and microorganisms are an
essential factor (Egli et al., 2011). Soil nutrients status is also determined by microbes
quality and their identification in the soil (Lombard et al., 2011). For soils' development,
microorganisms play a crucial role in improving soil fertility status and overall health.
Bacteria, actinomycetes, fungi, algae, protozoa, and viruses are all forms of soil
microorganisms. All organisms have unique traits that distinguish them from one
another and define their function in the soil. Importantly, these creatures do not exist in
isolation; they interact, and their interactions impact soil production equivalent to or
larger than the individuals' behaviour (Berg et al., 2010). Microorganisms are critical to
soil creation and soil ecology because they regulate the flux of nutrition to plants
(control of the biomass, sulphur, and nitrogen cycles), support nitrogen fixation, and
encourage the soil detoxification of inorganic material naturally stirring organic
contaminants. In addition, many bacteria exist in symbiotic relationships with plant
roots, creating a zone with the high microbial activity known as the rhizosphere (Zhu et
al., 2019).
The soil micro, meso, and macro-fauna have a beneficial association. For
example, protists, tiny nematodes, rotifers, tardigrades (microfauna) with a body size of
fewer than 100 m, springtails, detritivore, and predatory mites, proturans, symphylans
(mesofauna) with a body size of between 100 m and 2 mm, and insects such as ants,
carabids, termites, cicadas, and many species The majority of soil activities and
significant biogeochemical cycles are thought to be primarily governed by soil
microbial populations (FAO, 2017).
Earthworms, Nematodes, Acarina, Collembola, Mollusks, and various pests and
grubs feed primarily on decomposing organic materials and life. They're all part of the
soil food web, helping to move organic materials from the soil to fungal and bacterial
biomass. Soil microbiology is a young field, with just around 1% of soil microbes
described so far (Brown et al., 2000). Fungi and bacteria are heterotrophic organisms
that eat the micronutrients (zinc) and minerals released by decaying organic waste. As a
result, they're crucial to the nutrient recycling system that keeps soils healthy for plant
growth. One kind of soil's microorganism culture differs from another kind of soil's
microorganism culture (Bossio et al., 1998).
Microorganisms are present on soil surface and form “bio crust”. These bacteria
can alter soil chemical and biological features such as crucial nutrient cycling (i.e.
nitrogen and carbon), nitrogen and carbon fixation rates in soil, and soil fertility (Rossi
et al., 2015, Rashid et al., 2016). Soil aggregate stability, bulk density, particle density,
water holding capacity, soil organic matter levels, carbon, and nitrogen are all affected
by soil microbial activity and density (Veum et al., 2014).
Microbes help form the physical structure of soils by secreting organic chemicals
(mostly sugar) that act as "bonding agents" in the soil, glueing soil particles together
and forming soil structure. Other soil physical qualities are influenced indirectly by soil
microbes; for example, improved soil structure increases soil porosity, leading to more
significant air and water penetration. Soil microbes also create extracellular
polysaccharides or other cellular waste, which aid in the preservation of soil structure
and soil health (Wani et al., 2015). Colica et al., (2014) also found that when
microorganisms (Cyanobacteria) released polysaccharides, soil structure improved, and
soil hydraulic conductivity decreased. According to Rossi & De Philippis, (2015) the
development of biofilms by cyanobacteria is beneficial in enhancing soil's physical and
chemical properties. For instance, soil surface particle adhesion and carbon
sequestration in soil.
Important activities microorganisms in soil include: (1) soil organic matter
mineralization and nutrient cycling (Miransari, 2011a), (2) soil structure improvement
(Egamberdiyeva, 2007), (3) positive interaction with other soil microorganisms
(Miransari, 2011b), (4) production of biochemicals like plant hormones and essential
enzymes (Compant et al., 2010), and (5) suppression of plant pathogens (Mendes et
al., 2011).
Nitrogen is present in our environment, but it is not available for plant use.
However, some specific bacteria can capture this atmospheric nitrogen and transfer it
into the plant-available form. These microorganisms are known as “nitrogen fixation
bacteria”. This microbial process is most important for plants and the overall
agricultural system because it reduces chemical nitrogen fertilizers dependence.
According to estimation, the biological nitrogen fixation process produces nitrogen 200
million tons per annum globally. Soil microorganisms like bacteria have a symbiotic
relationship, and these are also known as “rhizobia”, including
genera Rhizobium, Bradyrhizobium, Mesorhizobium, and Sinorhizobium, with the roots
of leguminous crops which generate nodules that are responsible for atmospheric
nitrogen fixation by nitrogenase enzyme activity (Graham & Vance, 2003).
Some other soil functions are also driven by the diverse set of soil microorganisms
contributing to functional resilience like decomposition. Moreover, other relevant soil
functions are involved in more specific set of soil microorganisms, for example,
nitrifiers and bio-control agents. It has been observed that the makeup of the soil
organism community can affect numerous activities in the soil ecosystem, including
plant variety, decomposition, nutrient retention, and cycling (Semhi et al., 2008). These
micro and macro-organisms work together to improve soil quality. Macrofaunal
creatures play an essential role in the soil system as colonisers, comminutors, and
engineers, and their interactions with microbes are critical for this (Sofo et al., 2020).
The most effective bio-agent for improving soil health and fertility are the soil
microbes.
In Flowers
In flowers, the damage is also caused by microorganisms after harvesting,
primarily due to interruption of water contents because microorganisms start their
growth in the vas solution, so the xylem blockage is faced in the stored flowers stem.
Blockage of the branch is mainly caused by a microorganism and some other factors
like by production of extracellular polysaccharides and dead cells degradation. Bacteria
are also involved in the secretion of pectinases, some toxic compounds and ethylene,
accelerating the senescence process. It was also observed that besides reduction in vase
life, water relations disruption in the flowers (rose) could cause a problem in flower
opening, the flowers are opened improperly, or maybe flowers start to wilt (Jowkar et
al., 2012).
In Dairy Products
Bacteria that cause milk spoilage may be originated on-farm environment or the
milking equipment or in the processing plants, transferred from the kit, employees or
maybe from the air. When the lactic acid and some other compounds are produced, off-
flavour is milk is observed, especially when the microorganism’s population reach 106
CFU/ml. Refrigeration is helpful in lactic acid bacteria growth suppression. Still, other
psychrophilic bacteria, i.e. Pseudomonas, Alcaligenes, Enterobacter, and some spore-
formers, can grow within 25 hours and produce rancid odours (Dogan & Boor, 2003).
The process of pasteurization can kill both psychrophiles and mesophilic bacteria (lactic
acid bacteria). However, heat-tolerant species like Alcaligenes, Microbacterium,
Bacillus and Clostridium can survive and, in the end, cause milk spoilage and in other
dairy products (Rawat, 2015).
In Eggs
Under poor conditions of storage and management, the percentage of moisture in
eggshell is increased, making it favourable for the growth of fungal (mould) that cause
eggshells spoilage. When the fungal activities are grown on the surface of eggshells, the
pore size on the eggshell is increased, and the microbe’s entry through the shell
becomes easier. Types of spoilage can be characterized by egg colour very often
(Erkmen & Bozoglu, 2016).
Precautions
Nature uses microorganisms to carry out fermentation processes, and yeasts,
moulds, and bacteria have been used to produce bread, vinegar, beer, yoghurt, cheese,
and wine and fruit, vegetables, and fermented fish for thousands of years. Fermentation
is the earliest methods of food transformation and preservation. This biological process
increases the nutritional and organoleptic properties of food while still preserving it.
Sour cream, yoghurt, buttermilk, curd, bread, and cheese are some of the most popular
dairy products processed by microbes.
Bacteria, moulds, and yeast are the most common microorganisms that cause food
spoilage and are widely used in food and food product processing. For the fermentation
of dairy products, various bacteria and fungi are used to produce a broad range of
cultured milk products. In these cheese-making techniques, bacteria and fungi are also
included. Lactic acid bacteria coagulate milk, which can then be used to produce
various cheeses such as soft unripened, soft-ripened, semisoft, rugged, and very hard
types. Microbes such as Bifidobacterium and Lactobacillus are used in the food
industry. For example, alcoholic drinks and other beer products are made by fermenting
cereals and grains with various yeast strains.
We can prevent pathogenic infections by volatile organic compounds. Bio-
fumigation is a gaseous treatment; this can be used to get rid of this problem. Different
plants that produce volatiles can be used as potential substances for biofumigation
(Stelmach-Mardas et al., 2016). To prevent microbial growth in the vase solutions of cut
flowers, several compound/chemical like silver nitrate, silver thiosulphate, aluminium
sulfate, and sodium hypochlorite are considered beneficial (Liao et al., 2000).
Organisms like yeasts, i.e. Pichia guilliermondii, are used to control post-harvest
damages like rot in citrus and some other fruits. Like for the post-harvest apple, rot
prevention species of Cryptococcus and Acremonium breve are helpful. These are also
used to control rot in pear (Schnable et al., 2009). To preserve food and beverages, safe
additives that are anti-microbial are added, like acetic, sorbic, and benzoic acids
(Choudhary et al., 2017). Some other commonly used preservation agents are following:
acetic, lactic, benzoic, and sorbic acid. These are capable of inhibiting fungal and
bacterial cells outgrowth. Specially sorbic acid is utilized to inhibit bacterial spore’s
germination and outgrowth (Rawat, 2015).
Crops grains are preserved for a long time after their post-harvest, so these are
treated with some pesticides, drying, debranding, irradiation, and other ozone treatment.
Chlorine and hypochlorite are also helpful for this purpose (Los & Timmer, 2018). milk
spoilage is controlled by the process of pasteurization or sterilization and milk
dehydration. For dairy products, preservation of antimicrobial agents and some
generally regarded as safe (GRAS) substances have used that work as food additives,
stabilizers and emulsifiers (Verma et al., 2017). Eggs spoilage can be avoided if the
conditions are maintained during storage. By washing or applying disinfectants
(hypochlorite solution), microbes can be removed from eggs. Eggs pasteurization or use
higher or lower temperature treatments would conceal microbially (Erkmen & Bozoglu,
2016).
Future Opportunites
Among the most creative technologies, research and industry are increasingly
focused on the synthesis of microbial protein (MP) for regulated and intense systems
known as "bioreactors." MP has modest needs for arable land, does not compete directly
with crop-based food products and uses fertilizer at an efficiency of about 100%.
Furthermore, from an economic viewpoint, currently only in specific market segments,
such as aquaculture, use of MP in animal feeding is justified. Finally, the European
Food Safety Authority must approve innovative MPs before accessing the market.
Because of the externalised environmental problems of the existing agri-food
production system, the use of microorganisms as food will depend primarily on
favourable laws, public acceptability, and lower prices.
CONCLUSION
Microbes perform better functions for agricultural production by promoting
various direct and indirect mechanisms in soil and plants. Bacteria are the best plentiful
microbes in the ground, followed by fungi, actinomycetes, soil protozoa and soil algae.
A single gramme of soil can contain hundreds of millions to billions of microbes.
Therefore, if agricultural development is to satisfy the needs of an increasing global
population, a deeper understanding of soil microbiology is needed. Unfortunately,
agricultural activities continue to endanger the stable microbe population in many areas
rather than encourage the stable microbe population.
Furthermore, food decay and spoilage are caused by microbes. A rotten food
product has a foul odour, flavour and texture. Despite their pathogenicity, microbes play
a substantial role in dispensing fermented drinks and foods in the food industry and
home. Probiotics, fermented foods and alcoholic beverages are flattering extra popular
due to their health benefits and flavour. Microbes influence agricultural productivity
through a variety of overt and indirect processes in soil and plants. Bacteria, fungi,
actinomycetes, plant protozoa, and soil algae are the most abundant bacteria in the
ground. Bacteria, moulds, and yeast are the most common microorganisms that cause
food spoilage and are widely used in food and food product processing. A single
gramme of soil can contain hundreds of millions to billions of microbes and thus; can be
used for enhancing yield and growth directly and indirectly.
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