BIOLOGY IGCSE FAQ
Dhruv
DEFINITION QUESTIONS 1-2 MARKS
GOLDEN QUESTIONS 3-6 MARKS
GENERAL NOTES
Paper 6
       I know that you guys are studying at the last minute. As of writing this, I am sitting for May/June 2024. Best of luck guys!!
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DEFINITION QUESTIONS 1-2 MARKS
                                                              Diffusion
Movement of particles from high to low concentration down the concentration gradient as a
result of random motion.
                                                              Osmosis
Movement of water from high to low water potential down the water potential gradient
through a semipermeable membrane
                                       Active Transport
Movement of substances from low to high concentration through a semipermeable
membrane against a concentration gradient using energy from aerobic respiration
                                        Translocation
Movement of sucrose and amino acids in the phloem from source to sink via active
transport.
                                           Enzyme
Biological catalysts made out of proteins that increases rate of reaction while remaining
unchanged at the end of reaction.
                                   Transmissible Disease
Disease where pathogens can be transferred from one host to another
                                           Species
A group of organisms that can reproduce to make fertile offspring
                                          Pathogen
Microorganisms that cause diseases
                                          Excretion
Removal of waste products and excess material produced by metabolic processes from the
body through excretory organs
                                          Sensitivity
Ability to detect and respond to changes in the internal or external environment
                                            Drugs
Any chemical substance consumed that alters/modifies chemical reactions in the body
                                          Hormone
A chemical substance produced by a gland and carried by the blood, which alters the activity
of one or more specific target organs
                                     Sexual reproduction
Process where the nuclei of two gametes fuse to form a zygote (and the production of
offspring that are genetically different)
                                         Fertilisation
Fusing of nuclei from a male and female gamete
                                           Variation
Difference between different individuals of a species
                                       Genetic Mutation
Random change in base sequence of DNA
                                            Gene
Length of DNA that codes for a protein
                                            Allele
Alternate form of gene
                                            Organ
A structure made up of groups of different tissues, working together to perform a specific
function
                                         Sense organ
A group of receptor cells that work together to detect and respond to specific stimuli
                                          Pollination
Transfer of pollen grain from one plant to another from anther to stigma
                                         Deamination
Removal of the nitrogen-containing part of amino acid to form urea
                                         Homeostasis
Maintenance of constant internal environment
                                         Codominance
When both alleles for a gene equally contribute to the phenotype.
                                          Genotype
Genetic makeup of an organism in terms of alleles
                                          Phenotype
                         Observable features of an organismSpecies
Group of organisms that have similar genetic coding with minimal variations and can
reproduce to produce fertile offspring
                                          Population
Group of organisms of the same species living together in the same area at the same time.
                                          Community
Population of all the different species living in the same ecosystem
                                          Assimilation
Absorption of nutrients for cell usage
                                         Limiting factor
An environmental factor that limits life processes due to its shortage in the environment
                                         Transpiration
Loss of water vapour via evaporation on the surface mesophyll cells followed by diffusion of
water vapour through the stomata.
                                            Meiosis
The nuclear division of cells to produce daughter cells with half the number of chromosomes,
so haploid cells. The daughter cells are genetically different and are gametes.
                                            Mitosis
The nuclear division of cells that produces two genetically identical daughter cells with equal
and diploid number of chromosomes.
                                          Ecosystem
A unit containing all the communities of different organisms and their environments
interacting with each other.
                                         Trophic levels
The position of an organism on a food chain, food web, or an ecological pyramid.
                                    Sustainable Resource
A resource that is produced/replenished at the same rate that it is used up such that it does
not run out nor harm the environment.
GOLDEN QUESTIONS 3-6+ MARKS
                       How neurotransmitter/nerve impulse is passed
Vesicles containing neurotransmitters move to the cell membrane of the presynaptic
membrane and fuse their membrane with it. Impulse stimulates vesicles to release
neurotransmitter molecules into synaptic cleft. Vesicles release neurotransmitters.
Neurotransmitters diffuse across the synaptic cleft/gap and bind with the complementary
shaped protein receptors that are on the cell membrane of postsynaptic membrane, passing
electrical impulse.
                                   How does wilting occur
   1. Wilting occurs bc the plant has higher water potential than the soil, water is lost from
       cells via osmosis which moves from plant to soil
   2. Water transpires from leaf to atmosphere
   3. Plant loses water faster than it can gain
   4. Turgor pressure caused by water pushing against cell wall decreases
   5. Plant loses turgidity and becomes flaccid
   6. if not treated cells become plasmolyzed
                                 How to remove oxygen debt
When oxygen debt occurs, lactic acid is produced. Heart rate remains high to transport lactic
acid in the muscles to the liver. Breathing rate and depth of breathing increases to supply
oxygen. Lactic acid is broken down in the liver via aerobic respiration.
                                   Role of liver in excretion
The liver removes the nitrogen-containing part of amino acids via deamination to form urea
and amino acids which are then assimilated into proteins
                                   How do vaccines work
Vaccines contain inactive (dead or weakened) pathogens. After the vaccine, the pathogen
enters the body and triggers the immune response.
Lymphocytes produce memory cells to store information about the pathogen and produce
antibodies that are complementary to the shape of the antigen that are on the cell membrane
of the pathogen.
The antibodies bind to the antigen and form an antibody-antigen complex. The antibodies
neutralise the toxins produced by the pathogens or get clumped together and are marked for
phagocytosis.
                                  How cholera causes diarrhoea
Cholera bacteria stick to the lining of the small intestines that release toxins that cause
release of chloride ions that lower the water potential of the small intestine. Cells around the
small intestine have higher water potential and release water via osmosis into the small
intestine. This causes watery faeces.
                                  How proteins are made by a cell
   1.    Genes are transcribed into mRNA (DNA stays in the nucleus)
   2.    mRNA leaves nucleus and moves to cytoplasm and passes through ribosome
   3.    Ribosome translates mRNA into a protein by assembling amino acids
   4.    Order of amino acids is determined by base sequence of DNA/gene
                   Bright Light                                     Dim Light
     ● Circular muscles contract                    ● Circular muscles relax
     ● Radial muscles relax                         ● Radial muscles contract
     ● Pupil constrict (pupil size decrease)        ● Pupil dilate (pupil size increase)
                   Near Object                                      Far Object
     ●    Ciliary muscle contract                   ● Ciliary muscle relax
     ●    Suspensory ligament relax/slacken         ● Suspensory ligament
     ●    Lens becomes more convex/thicker             contract/tighten
     ●    Light is refracted more                   ● Lens becomes less convex/thinner
                                                     ● Light is refracted less
                      Explain how bacteria become resistant to antibiotics
   1. Antibiotics cause non-resistant bacteria to die leaving resistant bacteria. Resistant
       bacteria survive due to genetic mutations.
   2. Surviving bacteria reproduce and pass on their advantageous allele/genes that give
       the resistance trait to their offspring.
   3. Number of resistant bacteria increases.
   4. Natural selection makes bacteria become better adapted to antibiotics. Thus making
       them resistant.
                                       Pathway of the heart
   1.    (Starts as deoxygenated blood) Vena Cava
   2.    Right Atrium
   3.    Tricuspid (Atrioventricular) valve
   4.    Right ventricle
   5. Semilunar valve
   6. Pulmonary artery
   7. Lungs (becomes oxygenated blood)
   8. Pulmonary vein
   9. Left atrium
   10.Bicuspid (Atrioventricular) valve
   11.Left ventricle
   12.Semilunar valve
   13.Aorta
   14.To body (as oxygenated blood then becomes deoxygenated as body uses oxygen
                                 How water uptake occurs
When transpiration occurs, evaporation of water vapour causes a negative pressure that
creates a suction pull, drawing up water, also due to osmosis. Water molecules are attracted
to each by cohesive forces and adhesive forces between the walls of the xylem creating
continuous columns of water.
     Vasodilation (Temperature increase)        Vasoconstriction (Temperature decrease)
    ● Thermoreceptor detect temperature          ● Thermoreceptor detect temperature
       increase and sends impulse to the             decrease and sends impulse to the
       brain                                         brain
    ● Arterioles dilate, allowing more           ● Arterioles constrict, allowing less
       blood flow to skin surface to                 blood flow under skin to decrease
       increase heat loss to surroundings            heat loss to surroundings
    ● Sweat glands produce sweat, which          ● Sweat glands do not produce
       evaporates to cool down body                  sweat/produce less sweat
    ● Erector muscles relax, lowering hair       ● Erector muscles contract, raising
       so that it does not trap air particles        hair to trap air particles for insulation
                                                  ● Skeletal muscles cause shivering
                                                     which helps body gain heat energy
                                                     due to respiration
                  Inhalation                                    Exhalation
    ● Internal intercostal muscles relax         ● Internal intercostal muscles contract
    ● External intercostal muscles               ● External intercostal muscles relax
       contract                                   ● Volume of thorax decrease
    ● Volume of thorax increase                  ● Pressure of thorax increase
    ● Pressure of thorax decrease                ● Diaphragm relax
    ● Diaphragm contracts                        ● Rib cage goes inward and
    ● Rib cage goes upward and outward              downwards (back to original
    ● Air goes in                                   position)
                                                  ● Air goes out
           Glucose level increases                       Glucose level decreases
 ● Insulin is produced in the pancreas            ● Glucagon is stored in the pancreas
 ● Converts glucose to glycogen                   ● Converts glycogen to glucose
    (stored in liver)                              ● Increase glucose levels
 ● Decrease glucose levels
      Biological washing powders (why are they only effective in [insert] temperature?)
● Contain protease and lipase to remove protein stains and fat/oil
● Break down large insoluble molecules into smaller soluble molecules that can be
   washed away
● Only effect in [insert] temperature as it is optimum as more enzyme substrate
   complexes can be formed. Any temperature higher than optimum will cause enzymes
   in washing powder to denature.
               Active Immunity                              Passive Immunity
 ●     Longer process (Long term)                 ● Short process (Short term)
 ●     Produces memory cell                       ● Does not produce memory cell
 ●     Natural: Exposure to pathogens             ● From another source: Breastmilk,
 ●     Artificial: vaccines                          direct injection of antibodies
  Describe the stages in the reproduction of a flowering plant from self pollination to
                            fertilisation (0610/43/O/N/23)
● Self pollination is the transfer of pollen grain from anther to stigma of the same flower
   or different flower of the same plant
● Pollen grain germinate to form pollen tube
● Pollen tube carries male gamete and travels down the style to get to ovary
● Pollen tube grows down penetrates the ovule via micropyle
● Pollen tube bursts and releases male gamete to fuse with ovule to form diploid
   zygote
                                         Reflex arc
1.    Receptor detects stimulus
2.    Sensory neuron creates and transmits an impulse to spinal cord
3.    Relay neuron passes the impulse to motor neuron
4.    The motor neuron carries the response to effector
5.    Effector carries out about the response, muscle contraction for example
                 Why do food chains usually have less than 5 trophic levels?
Across each trophic level, energy is lost. 90% percent of energy is lost as growth, excretion
and movement (any other use of energy is also acceptable except growth and protein
synthesis). Only 10% of energy is transferred to each trophic level. There is not enough
energy to sustain 5 trophic levels.
 How are the proteins in the cattle manure converted to the type of ions plants can absorb?
   1. Proteins are broken down/decomposed into amino acids via decomposers (nitrogen
       fixing bacteria) OR Proteases break down proteins into amino acids
   2. Deamination by decomposers converts amino acids into ammonium ions
   3. Ammonium ions are converted into nitrite ions to nitrate ions via nitrifying
       bacteria/nitrification
            Explain how bacteria are genetically engineered to make human insulin
   1.    Human genes are cut using restriction enzyme to form sticky ends
   2.   Plasmid is cut using the same restriction enzyme to form complementary sticky ends
   3.    Human gene is combined with the plasmid using DNA ligase enzyme
   4.   To form a recombinant plasmid
   5.   Recombinant plasmid is re-inserted into the bacterium
   6.   Bacteria are grown in fermenters and allowed to reproduce
                        How is the growth of a plant shoot controlled?
(Phototropism)
Auxin is a growth hormone. Auxin will diffuse to the shaded side causing plant cells to
elongate and move towards sunlight
                                              OR
(Gravitropism)
Auxin is a growth hormone. Auxin will diffuse down the plant. It collects on the lower side of
the plant causing plant cells to elongate. The plant bends upwards.
                                       Urine formation
   ● Blood enters the kidney through the renal artery
  ● Blood flow branches out to blood capillaries in the nephron
  ● Blood enter the glomerulus through the afferent arteriole in high pressure
  ● Ultrafiltration: large molecules such as fat, blood cells, platelets, protein will remain in
     the glomerulus, and exit through the efferent arteriole
  ● Small molecules such as amino acids, water, urea, mineral salts, glucose pass
     through a partially permeable membrane into the bowman’s capsule.
  ● They pass through the proximal convoluted tubule, and all glucose, amino acids, and
     most mineral salts and water is selectively reabsorbed
  ● They move into the loop of henle and the distal convoluted tubule, where some water
     is also reabsorbed
  ● Excess water, urea, mineral salts pass out the collecting duct and into the renal
     pelvis as urine
  Describe how starch is broken down in the human digestive system so that it can be
                                absorbed in the blood
                                  (0610/42/M/J/24)
  ● Starch is broken down via mechanical digestion using teeth and the churning
     movement in the stomach
  ● Mechanical digestion increases surface area for faster enzyme activity
  ● Chemical digestion breaks down large insoluble substances into small soluble
     substances
  ● Salivary glands in the mouth produce amylase which breaks down starch to maltose.
  ● Maltose is broken down into glucose via the maltase found in the epithelium of the
     small intestine
GENERAL NOTES
  ●   Features of organisms
  ●   Tests, Digestion system, Enzymes
  ●   Plants
  ●   The Body, Reproduction
  ●   Environment
Features of organisms
 Kingdoms                            Characteristics                         Examples
Animal            ● No cell walls                                          ● Tigers
                  ● Multicellular                                          ● Fish
                                                                            ● Coral
Plant             ● Have cell walls made of cellulose                      ● Moss
                  ● Undergo photosynthesis                                 ● Ferns
                  ● Have chloroplasts                                      ● Mango
                                                                               tree
Fungi             ● Have strands of hyphae                                 ● Toadstool
                  ● No chloroplasts                                           s
Protista          ● Multicellular & unicellular                            ● Algae
                  ● Has a nucleus                                          ● Amoeba
                  ● Some undergo photosynthesis or feed on                 ● Euglena
                     organic substances
Prokaryotes       ●   Mostly unicellular                                   ● Virus
                  ●   No nucleus                                           ● Bacteria
                  ●   No mitochondria
                  ●   Free loop of DNA in cytoplasm
                  ●   Have flagellum to aid in movement
                  ●   Viruses have a protein coat, Bacteria have a
                       cell wall
  ● features of insects
         ○ three pairs of jointed legs , two pairs of wings , three body segments &
            antennae,compound eyes
  ● features of arachnids
         ○ four pairs of jointed legs , two body parts , simple eyes
  ● features of crustaceans
         ○ more than four pairs of legs , two body parts and compound eyes
  ● features of myriapods
         ○ many body segments , one or two pairs of legs in each segment , antennae
         ○ centipede ⇒ one pair ll millipede ⇒ two pairs
  ● features of monocots
         ○ narrow shaped leaves , parallel veins , one cotyledon in a seed , petals in
            multiple of three
  ● features of dicots
         ○ broad leaves , network veins on leaves , petals in multiple of 4 & 5 , two
            cotyledons
  ● features of bacteria
         ○ no nucleus , no membrane-bounded organelles & unicellular
         ○ has cell membrane and cell wall , has DNA strand and plasmid
  ● features of viruses
         ○ protein coat and genetic material , and NO cell walls ( so antibiotics doesn't
            affect viruses )
                               credit: @darumdarimda_ on discord
Tests, Digestion system, Enzymes
Edit: Chemical digestion is the breakdown of large insoluble molecules into smaller soluble
molecules via enzymes/chemical substances to be absorbed.
Plants
                           Plant cell adaptations
       Part                                 Adaptations
Waxy cuticle   Make leaf water proof, transparent to allow sunlight in
Upper epidermis       Transparent to allow sunlight in
Palisade mesophyll    Lots of chloroplasts to convert sunlight to chemical energy via
                      photosynthesis
Spongy mesophyll      Has a lot of air spaces to allow gases to diffuse into the plant easily
Vascular bundle       Xylem
                         ● Has lignin cell walls to ensure that it stays open and does not
                            collapse for support
                         ● Hollow with no organelles to let water in without resistance
                         ● Narrow to ensure that the column of water does not break
Stomata                   ● Has guard cells to open and close the stomata
                  (Limiting factors) Explain the rate of transpiration when:
   Temperature increases             Humidity increases          Light intensity increases
   ● Transpiration                  ● Transpiration             ● Rate of
      increases                         decreases                    photosynthesis
   ● Kinetic energy of              ● Concentration of             increases
      water vapour                      water vapour in           ● Transpiration
      increases                         atmosphere                   increases
   ● Diffuse faster into               around plant is           ● Stomata opens more
      stomata                           high                         in the light as it
   ● Stomata opens more             ● Lower                        remains open to allow
                                        concentration                gases (carbon
                                        gradient                     dioxide) in as a
                                     ● Lower rate of                reactant for
                                        diffusion                    photosynthesis whilst
                                     ● Stomata opens                allowing water to
                                        less                         escape as a product
                                                                     of photosynthesis
                                                   Function
Sepal      Protects flower when in bud
Petal      Attract pollinators
Ovary      Site of fertilisation, contains ovule
Anther     Produces pollen grains (male gametes)
Filament   Supports anther
Stigma     Receives pollen grains during pollination
Carpel     Female part of the flower
              Wind pollinated                                    Insect pollinated
No petal/dull petals                               Big and bright petals
Feathery stigma                                    Stigma is sticky
Pollen grains are small light and abundant         Pollen grains have spikes/hooks and is
                                                   produced in small amount
Anther is outside flower                           Anther is inside flower
Filament is long and sticky                        Small filament
No smell and nectar                                Sweet scent, has nectar
 Stigma outside of flower                      Stigma is inside flower
           Self pollination                                        Cross pollination
 Transfer of pollen grain from one       Definition      Transfer of pollen grain from one
 anther of a flower to the stigma of                     anther of a flower to stigma of a
 the same flower or a different                          different flower of the same
 flower from the same type of plant                      species
    ● No pollinator needed             Advantages           ● Increased biodiversity and
    ● Fast process                                             variation
    ● High success rate                                     ● Resistance to disease
    ● Isolated species can                                  ● Ability to adapt to change
       reproduce                                                in environment
    ● Less energy needed
    ● Lack of dispersal can           Disadvantages         ●   Long process
       cause competition                                     ●   Require pollinator
    ● No variation                                          ●   Wasting of gametes
    ● No adaptation to change in                            ●   Lower chance of success
       environment
    ● Offspring can become
       homozygous overtime and
       result in loss of gene pool
                  Xenophytes                                   Hydrophytes
    ● Thick waxy cuticle                         ● Contains large air spaces
 Reduce water loss                             More buoyant
    ● Spiky leaves                               ● No/little stomata in the lower
 Reduce water loss                                   epidermis
    ● Small number of stomata                    ● Roots are poorly developed and
 Reduce water loss                                   also has air spaces
    ● Deep and long roots                        ● Stems are weak as they don't need
 Able to access water sources deeper                 to support the plant
 underground, larger surface area of
 absorption of water
Source and sink
Sink: part of plant that is using or storing glucose and amino acids 
Source: part of plant that is producing (leaf when photosynthesizing esp. during summer) or
releasing (roots during spring) sucrose and amino acids
Plant adaptations
 Xerophyte (plants adapted to living in very dry, arid conditions)
 Stem                            Leaf                            Roots
 Swollen stem                    Needle-like to reduce           Shallow spreading roots to
                                 surface area                    catch rainwater
                                 Hairs in the lower epidermis    Deep to find water source in
                                 to catch water vapour           soil
                                 Waxy cuticle
 Hydrophytes (plants adapted to living in very moist, wet conditions)
 Stem                            Leaf                            Roots
                                 Large air spaces to float       Small and shallow since
                                                                 they can extract water
                                                                 through their tissues
                                 Stomata found in upper          No root hairs
                                 epidermis and usually open
                                 all the time
                             The Body, Reproduction
                                         Adaptations
 Septum          To separate oxygenated and deoxygenated blood
 Valves          Prevents backflow of blood
 Arteries            ● Takes blood out of heart
                     ● Small lumen, thick walls to carry high pressure
                        oxygenated blood
 Veins               ● Takes blood into heart
                     ● Large lumen
                     ● Has valves
                                                  Adaptations
Sperm              ● Acrosome: Contain packet of enzyme which breaks down the jelly coat of
                      the egg cell
                   ● Many mitochondria: More energy for swimming
                   ● Flagellum: Aid in movement (for swimming)
Egg                ● Jelly coat: Hardens to prevent sperm from entering after fertilisation
                   ● Energy/Nutrient stores: For development of embryo
                                                       Adaptations
Gas exchange                ● Large surface area to increase rate of diffusion
surface (Alveoli)           ● Good blood supply to maintain steep concentration gradient
                            ● One cell thick to reduce diffusion distance and increase rate of
                               diffusion
                            ● Moist so that gases can be dissolved and absorbed easily
Villi                       ● Microvilli to increase surface area
                            ● One cell thick to reduce diffusion distance and increase rate of
                               diffusion
                            ● Lacteal for absorption of lipids
                            ● Good blood supply to maintain steep concentration gradient,
                               quick access to bloodstream
Red blood cell              ● Biconcave shape to increase surface area for more oxygen
                               absorption
                            ● No nucleus so that it can hold more oxygen
                            ● Haemoglobin to bind onto oxygen
Trachea                     ● Cartilage to prevent airway collapse and protect trachea
Neurons                     ● Long/Have axon to carry impulses for long distances
                            ● Has many mitochondria that provide energy via aerobic
                               respiration for transmission of impulses
                            ● Has vesicles which hold neurotransmitter
                            ● Receptor molecules to pass and receive impulses
                            ● Branches to connect to other neurons
              Hormonal communication                           Nervous communication
        ●   Long term                                  ●   Short term
        ●   Multiple target organs                     ●   One target
        ●   By glands                                  ●   Conducted by CNS
        ●   Carried by blood                           ●   Carried by neurons
                              Advantages of double circulatory system
    ●   Maintain high blood pressure in systemic circulation and low pressure in pulmonary
    ●   Separates oxygenated and deoxygenated blood
    ●   Faster and efficient supply of blood and nutrients to body cells
    ●   Allows efficient filtration in kidneys
    ●   Maintains high metabolic rate in the body
    ●   Provide more time for gas exchange
                            Evidence of double circulatory system
    ● Blood flows through twice in one circuit
    ● Two separated blood circuits (pulmonary and systematic)
    ● 4 chambers with a septum
Shit to remember: Renal vein, renal artery, hepatic vein, hepatic artery, hepatic portal vein
(transports deoxygenated blood, glucagon & amino acids from the gut to the liver)
                        Selective reabsorption
   Substances        Amount absorbed           How it is reabsorbed
 Glucose            Everything             Active transport
 Water              Some                   Osmosis
 Urea & Toxins      None                   -
 Mineral salts      Some                   Active transport
 Amino acids        Everything             Diffusion
                                               Function
 Iris             Controls amount of light that enters pupil
 Retina           Contains light receptor cells (rod and cone cells)
 Fovea            Region of retina with highest density of cones. Light is
                  concentrated at this point.
 Lens             Transparent disc that helps to focus light on fovea by
                  changing shape
 Optic nerve      Sensory neuron that transmits impulses to brain
Part of eye       Number of cone cells            Number of rod cells
Blind spot    None                              None
Fovea         Many                              Few
Retina        Few                               Many
     Asexual Reproduction                                      Sexual Reproduction
                                   Definition
Process resulting in                                Process where the nuclei of two gametes
production of genetically                           fuse to form a zygote (and the production
identical offspring from one                        of offspring that are genetically different)
parent
     ● Good traits are           Advantages            ● Genetic variation
        passed down to                                  ● Increased biodiversity
        offspring                                       ● Ability to adapt to change in
     ● Faster than sexual                                 environment
        reproduction                                    ● Resistance to disease
     ● Can rapidly grow in                             ● Seed dispersal can reduce
        correct environment                                competition between parents and
     ● Uses less energy                                   offspring
     ● No gamete needed
     ● No adaptation to         Disadvantages          ● Difficult to reproduce when
        change in                                          isolated
        environment                                     ● Requires pollinators
     ● Offspring can                                   ● Takes longer
        become                                          ● Requires two parents
        homozygous
        overtime and result
        in loss of gene pool
     ● Lack of dispersal can
        cause competition
     ● Offspring can inherit
        bad traits
 Hormone          Where it is      Target           What it does        Inhibits    Stimulates
                  secreted         Organ
FSH            Pituitary gland   Ovary          Stimulates follicle        -       Oestrogen
                                                growth
LH             Pituitary gland   Ovary          Causes ovulation           -       Progesterone
Progesterone   Ovary/Placent   Uterus   Maintain pregnancy      LH    -
               a                           ● Thicken
                                               uterus wall
                                           ● No
                                               fertilisation?
                                               Causes
                                               menstrual
                                               cycle
Oestrogen      Ovary           Uterus   Repairs uterus          FSH   LH
                                        lining
                                  Environment
             Continuous variation                           Discontinuous variation
   ● Range of extreme values with                  ● Range of values with no
      intermediates                                    intermediates
   ● Affected by environment and genes             ● Affected by genes only and is
   ● Ex: Height, Weight, Muscle mass,                 inherited
      Hair colour                                   ● Example: Blood group, Gender, Ear
   ● Displayed via bell graph                         lobes, Ability to roll tongue
                                                    ● Bar chart
                               Genetically modifying crop plants
Advantages                                      Disadvantages
   -   Disease resistance                          -   Reduced natural biodiversity
   -   Higher yield                                -   GM seeds are expensive
   -   Herbicide resistance                        -   Ethical concerns (because you change
   -   More desirable product therefore more            nature or smth)
        income                                      -   Could cross pollinate into wild
   -   Adaptation to extreme conditions                 population
                                                    -   could cause pesticide resistant
                                                         weeds
        Eutrophication                      Monoculture                 Intensive Livestock
                                                                              farming
The process in which              Planting one species of crop   Raising large numbers of
excessive growth of algae         in an agricultural area at a   livestock in confined
occurs in a water body due to     large scale                    spaces to maximise
excessive minerals and                                           production
nutrients from sources such
as fertilisers
                                     ● Loss in biodiversity         ● Spread disease
   ● Fertilisers seep into          ● Increase in pest             ● Water pollution
      bodies of water                   number                       ● Reduced
   ● This causes growth of          ● Increased greenhouse            biodiversity
      producers such as algae.          gases                        ● Increased
      Results in algae bloom         ● Global warming,                 greenhouse
   ● This prevents oxygen              climate change                  gases
      from penetrating through       ● Habitat loss
      the water and dissolving       ● Disruption in food
      in the water.                     chain
   ● Sunlight cannot pass
      through, photosynthetic
      plants die
   ● When the producers start
      to die, the decomposers
      start to decompose the
      producers
   ● The decomposers
      respire aerobically which
      uses all the oxygen in
      the water, reducing the
      oxygen concentration in
      the water.
   ● All organisms die due to
      lack of oxygen
                         Consequences                            Prevention
Deforestation      ● Flood                            ● Replanting trees
                   ● Erosion                          ● Reserve forests/Conservation
                   ● Loss in habitat                     areas
                   ● Decrease in oxygen in            ● Laws to limit tree cutting
                      atmosphere                       ● Education
                   ● Loss in biodiversity             ● Stricter requirements in order to
                   ● Extinction                          gain licence to cut
                                                       ● Increase punishments for illegal
                                                          deforestation
                                         Cause                           Consequences
 Habitat destruction       ● Extraction of natural                   ● Loss in biodiversity
                              resources, land for housing,            ● Extinction
                              farming activity                        ● Habitat loss
                           ● Pollution
                           ● Oil spills
                           ● Eutrophication
          AI (Artificial insemination)                       IVF (Vitro fertilisation)
 - Used when animal is reluctant to mate in      - Females are given fertility drugs
 captivity                                       - Their ovaries releases multiple mature
 - Sperm samples are collected from the          ova, collected by laparoscopy
 males                                           - Ova are mixed with the male seminal fluid,
 - These samples are then artificially           allowing some sperm to fuse with the
 introduced into the female reproductive         - Fertilisation takes place in petri dishes
 system                                          - The dividing zygotes are introduced into
 - These samples can also be frozen and          the female’s uterus by inserting a tube into
 stored for later use.                           the cervix
                                          Paper 6
Chapters to focus on:
Cellular movement (Osmosis, Diffusion & Active transport)
Biological Molecules (Food tests & Enzymes)
Factors affecting photosynthesis & transpiration
Gas exchange
Safety Precautions:
   ● Keep away from flame
   ● Tie up long hair
   ● Wear a lab coat
   ● Wear goggles
   ● Wear gloves
   ● Keep a first aid kit
   ● Avoid splashing
   ● Handle equipment properly to ensure it does not drop on anyone
Possible errors & fixes
Error / Issue                      Fix / Equipment
Deciding colour changes by eye     Use colorimeter
Parallax Error                     Ensure to read measurements at eye
                                   level
Not using a thermostatically
controlled water bath to keep
temperature constant
Stirring manually and not mixing   Use stirrer
the solution entirely
-                                  Repeating experiment
-                                  Take more values to calculate an average
Leakage of air                     Close bung quickly
pH was not controlled              Use buffer solution
Cross contamination                Use different apparatus
Heat from lamp not constant        Use heat filter
Water evaporation                  Cover with lid or bung
                                          Food Tests
         Test name                        For what?                     Result
Biuret Reagent                  Protein                      Blue > Purple
Ethanol Emulsion                Fat/Lipid & Oils             Colourless > Milky
Add ethanol & water to food
sample, and with a mortar
Iodine                          Starch                       Brown > Blue
Benedict’s Solution             Reducing Sugar               Blue > Brick-red
(REMEMBER TO HEAT IN
WATER BATH AT 80°C)
DCPIP                           Vitamin C                    Blue > Colourless
Hydrogencarbonate               Carbon dioxide               Yellow (Acidic, most CO2)
indicator                                                    Red (Some CO2)
                                                             Purple (Alkali, no CO2)
                                     Drawing (4 marks)
1 mark: line, must be clear with no sudden cross sections and no shading
1 mark: scale, draw it BIGGGGG
2 marks: two smaller details on the drawing
                                           Graph
1 mark: Axis (WITH UNITS)
2 marks: Values (CORRECT UNITS
1 mark: Line (A CLEAR LINE)
                                    Experiment (6 marks)
Independent variable (What is changing? What is the experiment based on?)
Dependent variable (What do you need to calculate to get your results)
Controlled variable (What stays the same so the experiment is fair?)
Method:
1.
2.
3.
Safety: (Gloves, googles, etc)
remember to mention specific values and units (ex: 40°C, 5 cm^3)
                                 SAMPLE IGCSE EXPERIMENT
                                      0610/63/o/n/23
Humans can use the energy realised during respiration to exercise. Plan an
investigation to determine the effect of the exercising at different intensities on
breathing rate.
Independent variable: Intensity of exercise (Least to Most intense: Walking, Jogging, Jump
rope, Jumping jacks and Running)
Dependent variable: Pulse rate of participants
Controlled variable: Time duration of the exercise, gender and fitness level of participants
Method:
1. Have 5 participants of the same gender and fitness level
2. 5 participants do different exercises of the different intensities
3. Use a stopwatch and set it to 5 minutes, have the participants begin the exercise at the
same time
4. After 5 minutes, measure the pulse rate of the participants for 1 minute
Repeat experiment to obtain average results
Safety: Stretch beforehand to reduce risk of injury, and do exercises carefully.