BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

• Smallpox Throughout history,

millions of people killed.
• Many years as a result of a
smallpox vaccine is engraved
in the earth. Somalia last had a
natural death in 1977.
• Virus in laboratory cultures
of 70 samples were stored in the
same year in the world.
•Studies in the Faculty
of Medicine at the University of
Birmingham in
1978, virus escape the
lower floor of the laboratory
(Imaging Section dark room)
charge caused the death
of Janet Parker.
• Prof. Henry Bedson h
olds himself responsible for this
incident and committed
suicide by cutting her throat.
• In 1978, WHO has ordered the
destruction of all
existing cultures.
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

WHAT WE WANT TO PROTECT THE LABORATORY WORK?

• Environment
• The staff, everyone working in the laboratory
• Sample of the product so we're working on.

DETERMINATION OF THE LEVELS OFLABORATORY BIOSAFETY

Determination is based on 4 main elements :
• Work on micro-organisms and their classes of danger, mixable risk in
the form of aerosol into the air., treatment facilities, and so on.
•Standard microbiological practices
• Biosafety equipment
• Laboratory infrastructure
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

BSL-1
 Standard microbiological procedures are applied,
 Not a special barrier,
 The sink for hand washing,
 The disease is known that the risk of micro-
organisms, is unworked laboratories.
Example: Water testing labs,training labs

BSL-2
 Moderate-risk, non-invasive form of aerosol,mainly the skin, mucous
membrane contact with the disease or the risk of swallowing the
microorganisms studied Laboratories.
 Aerosol spread or biosafety cabinets should operate with a high risk of
splashing.
 Barriers should be used, and decontamination systems.
Example: Clinical, diagnostic, Educational Laboratories
Example microorganisms: C. difficile, hepatitis A, B, C, influenza A, Lyme
disease (tick-borne),dengue disease, salmonella, mumps, measles,HIV, mad
cow disease, MRSA, genetically modified organisms constitute a direct
threat to human.
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

BSL-3
 High-level risk, aerosol form that can spread the risk
of serious and fatal disease of the laboratories working with microorganisms.
 Independent of other units,
 Barriers gradually, controlled access, appropriate
monitoring and recording systems must be installed.
 All laboratory procedures must be made within the appropriate class of
Biosafety Cabinets.
 Always special clothing, gloves, mechanical pipette should be used, with a risk
of splash safety glasses should be worn operations.
 Single-pass ventilation system, with appropriate filtration, the
critical filters should be designed to be done fumigation.
 Room with appropriate pressurization system as a negative pressure must
be continuous. The first barrier to entry of outside air with
positive pressure should be avoided.
 Gas-tight room, the floor must be able to spill-
resistant. Pipe and cable entries allow this feature to the detriment the
room should not be given.
 Wall, ceiling and floors should be hygienic.
 All waste must be decontaminated.
Example: Bird flu, Recombinant DNA, and so on. Research Laboratories
Example microorganisms: tuberculosis,anthrax, West Nile
virus, Venezuelan equineencephalitis virus, western equine encephalitisvirus, H
endra virus, SARS, salmonella typhi,coxsiella burnetii, Rift
Valley fever virus, birdflu.
BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

BSL-4
 A high level of risk of death without treatment or vaccine,
which is distributed over the form of aerosol,
 Laboratory personnel, not only for the case of
propagation of risk to the community that are involved
in all laboratories working with microorganisms.
 Only BCS class 3 cabinets that can be workingor positive-
pressure full-body-type clothing ,
 Very special and complex ventilation systems,
and waste decontamination in the Laboratories.
Example: Ebola, SARS, and so on. Research Laboratories
Example viruses : Bolivia, and Argentina
hemorrhagic flu, Marburg virus, Ebola virus,Lassa flu,
Crimean-Congo hemorrhagic fever,flowers, and so on.
BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

Different biosecurity examinations requested features:

BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

MAIN LABORATORY VIEW

 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
2- Ventilation-air conditioning
Architectural features:
system
General 3-Waste management
• Laboratory work, must have sufficient space for
cleaning and maintenance.
• All personnel and material inputs and outputsmust be
under the control and registration.
• Wall, ceiling and floor :
•Continuous, non-porous, easy to clean, dust-
producing, anti-dust.
• Particulate and microbiological settlement that does not
allow.
• Resistant to disinfectants and cleaning materials.
• Ground will be a non-slip construction.
• Inaccessible areas to reduce the accumulation
of dust and clean it will not allow. Rack and so
on. projection areas of the elements to be minimized.
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

Architectural features : BSL-3 LABORATORY FEATURES

The lab must have adequate lighting, glare and so on. should not be. 1- Architectural features
2- Ventilation-air conditioning
•Labs should have hand wash ,eye shower, emergency response system
equipment. 3-Waste management
• Floors must be liquid-tight. the space must be gas-tight. For
this purpose, gas-tight inflatable door seals, gas-
tight doors, and so on. is available.
• If required, all the laboratory-might be
appliedfumigation process.
• The main materials that meet these specifications:
• Wall / ceiling
• HPL (compact laminate) or stainless
steelclean room panels,
• Epoxy paint
• Floor,
•Epoxy,
• Antibakterial PVC
• Maintain the balance of pressure and air-Locksmust be necessary to
prevent cross-contamination.
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
2- Ventilation-air
conditioning system
3-Waste management
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

BSL-3 LABORATORY FEATURES

1- Architectural features
2- Ventilation-air conditioning
system
3-Waste management
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

Pendik BSL-3 air flow directions and positions of the

gas-tight doors
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
2- Ventilation-air conditioning
system
3-Waste management
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
Air Conditioning System 2- Ventilation-air conditioning
system
3-Waste management

• Air Conditioning system should be a single-pass. In other words, recirculated air should not
be used.
• Laboratory must be continuous negative pressure..
• This pressure to maintain the continuity of supply and exhaust fans must interlock.
• Filter fans, regardless of the fixed flow rate should provide.
• Critical elements must be regularly backed up.
• Exhaust system must be HEPA (High-Efficiency Particulate Air) filtered.
• Exhaust HEPA filters and all the critical sections, must be possible to fumigate.
• Of each room, VAV systems must be equipped. VAV can control the amount of supply
and suction.
• All critical operations must be made within the appropriate class of Biosafety cabinets.
• Pressures should be monitored continuously, in case of loss of pressure alarms must trip.
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
Air Conditioning system schematic diagram. 2- Ventilation-air
conditioning system
3-Waste management
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
The amount of particles allowed Air 1- Architectural features
2- Ventilation-air
Conditioning system conditioning system
3-Waste management

STANDARDS TO ALLOW THE MAXIMUM NUMBER OF PARTICLES

CLASSES PARTICLE AMOUNT ALLOWED

FDA ISO GMP 0.5 μm/ft3 0.2 μm/m3 0.3 μm/m3 0.5 μm/m3 5 μm/m3

100 5 A 100 26,500 10,000 3,500 Undefined

1,000 6 B 1,000 Undefined Undefined 35,000 250

10,000 7 C 10,000 Undefined Undefined 350,000 2,500

100,000 8 D 100,000 Undefined Undefined 3,500,000 25,000

Microorganisms that can generate the colony, in the air can not move itself. Use of
a particle as a host. Particle size that can be used for this work, usually 0.5
micron and higher. For this purpose classifications are made according to the
number of particles 0.5 microns.
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
Air Conditioning system used filters and the 1- Architectural features
amount ofparticles spread the employees. 2- Ventilation-air conditioning
system
3-Waste management
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
Air Conditioning system schematic diagram. 2- Ventilation-air conditioning
system
3-Waste management

The key points :

•Air handling
unit and exhaust HEPA filters
are redunant.
• There are gas-tight dampers and
fumigation valves at
exhaust HEPA filters entries and
exits to not
allowing for fumigated.
•Redundant automation units, the
transition from ones equence to
another must be taken into
account in risk analysis. The
fans, which is important to
turn trapped by the
capacity curve.
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
Air Conditioning system schematic diagram. 2- Ventilation-air
conditioning system
3-Waste management
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
Fumigation can be done, redundant HEPA 1- Architectural features
and P & IDdiagram of the room. 2- Ventilation-air conditioning
system
3-Waste management
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
Hands-off as a replaceable HEPA filter scheme and the atmosphere. 1- Architectural features
2- Ventilation-air conditioning
system
3-Waste management
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
Air Conditioning system 1- Architectural features
2- Ventilation-air conditioning
Air handling unit features: system
3-Waste management
The outer casing

•Tight and rigid structure,

•Double walled, stainless steel inner wall.

• Panel thickness min. 25 mm, high-density rock wool insulation,

• Inner surface hygienic standards. unindented - ridged structure.

• Filter and fan cells, control windows and interior lights.

• Each filter is available for the manometer and electrical alert,

• Fans have frequency inverter. value of the minimum efficiency of

75%.

•Fan and motor epoxy paint,

• Heating - cooling battery speed of 2.5 m / s, at least

2.4 mm fin spacing

• PPTV or stainless steel drop-retaining.

• Stainless steel condensatepan.

 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
The rooms flow-pressure control, 1- Architectural features
Automatic flow control equipment (VAV) 2- Ventilation-air conditioning
system
3-Waste management

BSL-2 BSL-3
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
All transactions should be made within Class II type B2 biosafety 1- Architectural features
cabinets. Exhaust connection should be made directly.(without a paddle) 2- Ventilation-air conditioning
system
3-Waste management
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
The rooms flow-pressure control, 2- Ventilation-air conditioning
Automatic flow control equipment (VAV) system
3-Waste management

High bio-security studies, the flow rate and pressure were held constant, is of great
importance in its class.
For this purpose:
• Air handling unit and exhaust fan flow rate /pressure is kept constant,
• Air flow is controlled by the amount of each room.

• The main problems in fixing the pressure of the room: the door opened, and off to disable
the glass in the biosafety cabinet. Supply circuit, constant flow rate can be solvedwith the
application relatively easily. However, the time constants of automation ofthe control of the
exhaust system is of great importance. Frequency inverter fan motor time constant, the
opening-closing speed of the glass cabinet and VAV response time is required to
becompatible with each other.
• Room pressure must always be negative. For this purpose, the
supply / exhaust fan interlock system, and so on. all measures should be taken.
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
The rooms flow-pressure control, 1- Architectural features
Automatic flow control equipment (VAV) 2- Ventilation-air conditioning
system
3-Waste management

Venturi-type VAV device features:

• Even after the elbow can be used immediately,in front of and behind
the flat channel is not required.

• Response time is 1 secshorter.

• Approximately 3-fold highersensitivity.

• Max / min flow rate ratios 50:1

• Max / min pressure differences: 150/750 Pa

• Body, the complete-tight welded.

•Expensive !!!
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
The rooms flow-pressure control, 1- Architectural features
Automatic flow control equipment (VAV) 2- Ventilation-air conditioning
system
3-Waste management

Clapper-type VAV device features:

• Channel must be straight in

front and behind.

• Response time is 30
sec electricalaccumulators. May be
shorter than 5 secpneumatic ones.

•Max / min flow rate in pneumatic 5:1. If el

ectricis 8:1.

• Max / min pressure

differences: 200/800 Pa

• It is cheaper.
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
Otomasyon 1- Architectural features
BUILDING MANAGEMENT SYSTEM POINT DISTRIBUTION TABLE 2- Ventilation-air conditioning
BYS

S-NO
SYSTEM

EXPLANATION PCS.
SITE MATERIAL

TYPE DI
INLET OUTLET POINTS

DO AIP AIV AIA AOV AOI

system
R&D LABORATORY AIR HANDLING UNIT 3-Waste management
AHU-8

(TYPE B)

4 SUPPLY FAN FI ON/OFF CONTROL 1 1

4 SUPPLY FAN MOTOR CURRENT INFO (4-20mA) 1 1

4 SUPPLY FAN FI FAULT 1 1

4 SUPPLY FAN DRIVE BELT BROKEN 1 QBM81-5 1

4 SUPPLY AIR VOLUME INFO (4-20mA) 1 * 1

4 SUPPLY AIR DİF.PRESSURE INFO 1 QBM62.203 1

4 SUCTION FAN FI ON/OFF CONTROL 1 1

4 SUCTION FAN MOTOR CURRENT INFO (4-20mA) 1 1

4 SUCTION FAN FI FAULT 1 1

4 SUCTION FAN (WITHOUT BELT) 1 - - - - - - - -

4 RETURN AIR VOLUME INFO (4-20mA) 1 * 1

4 RETURN AIR DİF.PRESSURE INFO 1 QBM62.203 1

4 FILTER-1 PLUGGED ALARM 1 * 1

4 FILTER-2 PLUGGED ALARM 1 * 1

4 FILTER-3 PLUGGED ALARM (SUPPLY AIR) 1 * 1

4 FRESH AIR TEMPERATURE AND HUMIDITY INFO 1 QFM65 2

4 SUPPLY AIR TEMPERATURE AND HUMIDITY INFO 1 QFM65 2

4 RETURN AIR TEMPERATURE AND HUMIDITY INFO 1 QFM65 2

4 SUCTION FAN OUTLET AIR TEMPERATURE AND HUMIDITY INFO 1 QFM65 2

4 COOLER VALVE MOTOR POSITIONING (0-10 VDC) 1 * 1

4 COOLER VALVE MOTOR POSITION INFO (0-10 VDC) 1 1

4 COOLING COIL RETURN WATER TEMPERATURE 1 QAE22A 1

4 COOLER CIRCULATION PUMP ON/OFF CONTROL 1 1

4 COOLER CIRCULATION PUMP FEED BACK 1 1

4 HEATER VANA MOTOR POSITIONING (0-10 VDC) 1 * 1

4 HEATER VANA MOTOR POSITION INFO (0-10 VDC) 1 1

4 HEATER COIL RETURN WATER TEMPERATURE 1 QAE22A 1

4 FROST ALARM 1 * 1
 5
4
3
2
1
NO

TOTAL
UPPER FLOOR
UPPER FLOOR
UPPER FLOOR

LOWER FLOOR

ENERGY BUILDING
SITE STATION NAME

4
40
16
25
26

111
DI POSITION

0
6

48
15
12
15
DO SWITCHING

6
4
6
6

36
14
AI PASSIVE MEASUREMENT (Ni 1000)

0
49
24
36
36

145
AI ACTIVE MEASUREMENT (0..10VDC)

0
8

50
18
12
12
AI ACTIVE MEASUREMENT (4..20mA)

0
8
6
8
7

29
AO POSITIONING (0..10VDC)

0
0
0
0
0
AO POSITIONING (4..20mA)
Automation

18
64
99

419
136
102
TOTAL POINT

0
MEC 200 549002

0
MEC DIGITAL BLOC 549201

0
MEC ANALOG BLOC 549203

1
4
7
7

29
10
PTM6.4D20

0
8
3
6
8

25
PTM6.2Q250-M (Manuel command)

7
3
2
3
3

18
PTM6.2R1K

73
25
12
18
18

PTM6.2U10

0
9
4
6
6

25
PTM6.2I420

0
4
3
4
4

15
PTM6.2Y10S-M (Manuel command)

0
0
0
0
0
0

PTM6.2Y420-M (Manuel command)

8
59
28
44
46

185
MODÜL TOPLAMI

0
RBC (12 modül) 545287

3
1
1
1

MBC-24 (20 modül) 545077

5
2
1
1
1
BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE

MBC-40 (36 modül) 545078

2
1
1

CONTROLLER (3 MB) 545718

3
1
1
1

CONTROLLER (4 MB) 545719

3
1
1
1

MASTER / SLAVE PTM6.EMK

8
1
2
1
2
2

POWER MODULE 545714

3-Waste management

5
1
1
1
1
1

ADDRESS SWITCH 545825

BSL-3 LABORATORY FEATURES
1- Architectural features

5
1
1
1
1
1

ADDRESS SWITCH 545826

4
1
1
1
1

ADDRESS SWITCH 545827

4
1
1
1
1

ADDRESS SWITCH 545828

3
1
1
1

ADDRESS SWITCH 545829

2- Ventilation-air conditioning system
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
Waste: 1- Architectural features
Everything that went out of the lab should 2- Ventilation-air conditioning
be decontaminated. system
3-Waste management
•The exhaust line to spread risk to the environment, can
be fumigated withHEPA filters must be decontaminated.
• Any type of solid waste and materials, after
being sterilized by autoclaveor fumigation chamber must
come out of the laboratory.
• All liquid wastes, that allows heat
or chemical decontamination must be disposed of after
being neutralized by taking decontamination tanks(kill
tanks).
 BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
Chemical Decontamination 2- Ventilation-air conditioning
system
3-Waste management
BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
2- Ventilation-air conditioning
system
3-Waste management
BSL-3 LABORATORYDESIGN-CONSTRUCTION GUIDE
BSL-3 LABORATORY FEATURES
1- Architectural features
2- Ventilation-air conditioning
system
3-Waste management