199 609 1 SM
199 609 1 SM
*** School of Psychology, University of KwaZulu-Natal, PO Box 54001, Durban, 4000 South Africa
* <mehtam@ukzn.ac.za>
** < pauleyes@mweb.co.za>
*** < bhagwanjeea@ukzn.ac.za>
stereopsis is used to evaluate horizontally dis- nisms11, it is important to investigate the effect of
parate images in contour stereotests such as the these tinted lenses on stereopsis over a range of illu-
Titmus Fly Stereotest (TFS)6, 21. Stereopsis may mination levels. Tinted lenses are commonly avail-
be measured at distance and at near. In a study able in a range of colours as either gradient or solid
by Wong et al where monocular cues were elimi- tints from grade A (light) to grade D (much darker).
nated and where they used the same tests at all Illumination levels may be consistently decreased
distances, they found no significant change in by using Gulden neutral density filters (ND) to al-
stereoacuity with viewing distance21. As this ste- low for measuring stereopsis ranging from room il-
reoacuity is a threshold value for the distance at lumination of 300 lux to measuring stereopsis under
which it is measured, stereopsis is not effective decreased light levels (<300 lux)24, 25.
beyond a certain critical distance3, 21. The aim of the study was to investigate the ef-
An important aspect of visual information re- fects of reduced illumination and tinted lenses on
lates to the perception of colour provided by the stereopsis at near. The specific objectives were as
three types of photoreceptors in the retina. Light follows:
is interpreted as colour according to the wave-
lengths that strike the retina18. When light reaches i. to compare stereoacuity values across five lev-
a surface, it undergoes three main changes. Some els of illumination (No ND filter, 0.3 ND, 0.6
of the light is reflected, some absorbed and the re- ND, 0.9 ND and 1.2 ND) without any lenses.
mainder transmitted18 - 20. A tinted lens possesses ii. to compare stereoacuity values using a white
a definite colour and acts as a filter that alters the lens against a no lens condition across the five
intensity and the spectral distribution of light that levels of illumination.
passes through it17 - 19. The colour of the tint is as iii. to compare stereoacuity values using six tinted
a result of chemicals added to the spectacle lens lenses at grade B against the no lens condition
to alter the transmission and absorption of the dif- across the five levels of illumination.
ferent wavelengths of visible light19, 23 - 25.
Optometrists frequently prescribe tinted lens- Methodology
es for their patients. Tinted lenses are prescribed, Selection of participants
inter alia, in the following circumstances: for the A purposive sampling procedure was used to
relief of photophobia26 - 28, to reduce asthenopic select sixty participants from the private practice
symptoms17, 26, to decrease light scatter in condi- of the clinician researcher (P Ramkissoon). This
tions such as albinism, retinitis pigmentosa (RP) purposive sample comprised a non-probability
and cataracts29, to provide protection for people cohort of participants who satisfied the inclusion
exposed to high levels of invisible radiation such criteria for this study. Adult participants ranging
as ultra-violet and infra-radiation19, to alleviate in age from 17 - 29 years were selected. Older
sensitivity to sunlight23, 24, to improve the cosme- adult participants were excluded in order to con-
sis in situations of disfigurements18, to enhance trol for age-related changes in vision. A compre-
visual ergonomics17, to reduce glare from reflec- hensive eye examination (including measures of
tive surfaces such as snow, sand and water19, 23, binocularity, that is, the cover test and fusional
as a placebo28, to improve colour perception in vergences) was used to screen all eligible par-
colour deficient individuals30 and to decrease ticipants to ensure that they satisfied the inclu-
light sensitivity in patients taking photosensitis- sion criteria for this study. Participants who had
ing drugs3. < 0.50 D of astigmatism and < 0.50 D sphere and
Since different colour tints are generally used monocular and binocular visual acuities of 6/6 or
by patients under varying levels of illumination, better at distance and near were included, thus
ranging from bright sunlight to very poor light excluding bias accruing from compromised visu-
levels and in keeping with the more recent infor- al status. Participants with eye diseases and those
mation regarding chromatic stereopsis mecha- who failed the Ishihara colour test were similarly
excluded. Other exclusion criteria were poor favourably to two randot tests under compro-
general health status and intra-ocular pressure mised levels of binocularity in young partici-
above 21 mmHg. There was no evidence in the pants32. Provided that the page is inverted to re-
literature suggesting the need to control for race verse the disparity, the TFS controls adequately
and gender of participants. for the presence of monocular cues31. Further,
the TFS is a widely used clinical test that allows
Lenses used for rapid testing without significant subject fa-
A white CR 39 lens and six tinted lenses tigue, thereby yielding data that could inform
(CR39) were used on all participants in the clinical management decisions31. In the TFS,
study, namely, pink, blue, brown, grey, yellow horizontal disparity is presented via the vecto-
and green. The depth of the solid tints used in graphic technique33. This test comprises three
this study was grade B. All the lenses were of subtests, with the level of disparity decreasing
zero power (plano) with a 2 mm standard centre progressively33. When tested at 40 cm the first
thickness and equal transmittance, as measured subtest (the fly) has a disparity of 3600 sec of
on a spectrophotometer. Thus, all pertinent lens arc, the second subtest (the rows of animals) has
characteristics, including lens material, depth of a disparity ranging from 400 - 100 sec of arc and
tint, power, thickness and transmittance were the third subtest (the Wirt rings) has a disparity
controlled for in order to eliminate secondary ranging from 800 - 40 sec of arc6. The ultimate
variance. Stereopsis was assessed, for each measure of stereopsis is the participant’s ability
participant, using a no lens condition and seven to discern the finest possible level of disparity.
other lens conditions. Given these considerations, the TFS was con-
sidered the measure of choice for this sample.
Illumination levels
Neutral density (ND) filters have been wide- Procedure
ly used in photography, cinematography and Phase 1: Stereopsis measured under room illu-
vision research to assess visual function under mination of 300 lux with no ND filter
controlled levels of illumination24, 25. Stereopsis Stereopsis was measured initially without any
was initially assessed under bright room illumi- lenses (base line measure) followed by measure-
nation of 300 lux measured using a light meter, ments through the white lens and then through
with ND filters (0.3 ND, 0.6 ND, 0.9 ND, and each of the six tinted lenses, under normal room
1.2 ND) being subsequently used to decrease illumination, using the TFS. The order of pre-
the level of illumination. Thus, stereopsis was sentation of tint condition was randomised to
assessed, for each participant, at five different avoid treatment interaction effects. As recom-
levels of illumination. mended, this test was held at a test distance of
40 cm measured using a RAF rule. The partici-
Stereoacuity test used pants viewed the targets binocularly using a pair
Standard clinical tests for stereopsis include of polarising spectacle lenses.
the TNO random-dot stereotest that does not The first subtest, the fly, was shown to the
have monocular cues based on the presence of participants who were asked to pinch the edge
contours, as well as the Titmus Fly Stereotest of the wings of the fly. The normal response
(TFS) that utilizes real contours, resulting in was that the pinching fingers should be off the
the presence of monocular cues. While the use page by several centimetres.
of randot stimuli are generally considered the In the second subtest comprising the three
“gold standard” for measuring stereopsis, they rows of animals, the participants were informed
are prone to false negative errors31. The TFS that all except one of the figures in each row re-
on the other hand has been shown to compare mains flat. The participants were asked to in-
dicate the animal that appeared raised in each
ence in stereopsis between the no lens and variations in the level of illumination, but varia-
white lens conditions at each level of illumi- tions in the spectral composition of light35. The
nation. response of the visual system (spectral sensitivity)
3. stereopsis was significantly superior with the to different light levels and colours is an inherent
no lens condition compared to all six other property of the type and distribution of the photo-
tint conditions (grade B), at each level of il- receptors and neurons across the retina. Based on
lumination. the difficulty experienced in seeing depth in isolu-
minant random-dot stereograms it was postulated
Discussion and Conclusions that stereopsis is “colour-blind” and that therefore
Information regarding the visual world is de- the information is most probably carried by the
duced based on the changes in the quantity and magnocellular system35. However more current
quality of light from a source and its interaction information suggests that there are two stereopsis
with matter such that one has to consider not only mechanisms, one sensitive to luminance contrast
Table 2: Multivariate Analysis of Variance of Main Effects (Tint an Illumination) and Interaction Effects on Stereoacuity
Source (Sphericity Type III Sum of df Mean Square F Sig.
Assumed) Squares
illum 202129922.333 4 50532480.583 119.706 .000
Error (illum) 99624752.667 236 422138.782
Table 3: Post-hoc Analysis: Familywise paired t-tests for Illumination by Tint(Mean Stereoacuity)*
Pairings Mean Std. Deviation Std. Error t df Sig. (2-tailed)*
Illumination Tint
No ND no lens - white -.333 2.582 .333 -1.000 59 .321
no lens - pink -3.667 7.584 .979 -3.745 59 .000**
no lens - blue -4.167 5.612 .725 -5.751 59 .000**
no lens - brown -3.500 6.846 .884 -3.960 59 .000**
no lens - grey -4.667 7.471 .965 -4.838 59 .000**
no lens - yellow -3.509 6.932 .902 -3.908 59 .000**
no lens - green -3.500 6.915 .897 -3.927 59 .000**
0.3 ND no lens - white -.500 7.686 .992 -.504 59 .616
no lens - pink -9.000 12.849 1.659 -5.426 59 .000**
no lens - blue -9.333 12.604 1.627 -5.736 59 .000**
no lens - brown -8.667 13.957 1.802 -4.810 59 .000**
no lens - grey -8.500 14.001 1.807 -4.703 59 .000**
no lens - yellow -7.333 16.351 2.111 -3.474 59 .001**
no lens - green -8.333 13.550 1.749 -4.764 59 .000**
0.6 ND no lens - white -3.667 14.018 1.810 -2.026 59 .047
no lens - pink -35.667 64.318 8.303 -4.295 59 .000**
no lens - blue -35.333 52.253 6.746 -5.238 59 .000**
no lens - brown -34.333 52.863 6.825 -5.031 59 .000**
no lens - grey -36.667 63.691 8.222 -4.459 59 .000**
no lens - yellow -30.667 31.346 4.047 -7.578 59 .000**
no lens - green -41.667 68.325 8.821 -4.724 59 .000**
0.9 ND no lens - white -15.333 58.729 7.582 -2.022 59 .048
no lens - pink -109.667 165.887 21.416 -5.121 59 .000**
no lens - blue -128.000 172.173 22.227 -5.759 59 .000**
no lens - brown -131.333 177.761 24.769 -5.693 59 .000**
no lens - grey -104.667 168.719 21.781 -4.805 59 .000**
no lens - yellow -98.667 142.988 18.460 -5.345 59 .000**
no lens - green -114.333 135.651 17.512 -6.529 59 .000**
1.2 ND no lens - white -16.667 92.364 11.924 -1.398 59 .167
no lens - pink -283.333 446.898 57.694 -4.911 59 .000**
no lens - blue -443.333 802.616 103.617 -4.279 59 .000**
no lens - brown -443.333 793.270 102.411 -4.329 59 .000**
no lens - grey -425.000 840.223 108.472 -3.918 59 .000**
no lens - yellow -270.000 550.901 71.121 -3.796 59 .000**
no lens - green -430.000 714.807 92.281 -4.660 59 .000**
* α = .001 (.05 divided by 35 paired t-tests)
** t-test values that meet the significance criterion
rying the information along the chromatic chan- change in stereoacuity induced by tinted lenses
nels and amongst other optical explanations, a will influence visually guided performance.
selective reduction of short-wavelength light has Luria39 also found that depth perception was di-
been posited37-39. This study indictates the need minished with tinted lenses; his proposal that the
for further research to investigate between-colour reduced transmittance produced by tinted lenses
comparisons using a range of tints and depths of decreased the stereoacuity is corroborated by the
tints as well as photochromic lenses under differ- findings of this study. Therefore, the practitioner
ent levels of illumination. should ensure that tinted lenses prescribed do not
Since the white lens performed as well as the retard stereoacuity in circumstances where good
no-lens condition under the different levels of il- stereoacuity is required3. In advising a patient
lumination, but both differed statistically signifi- concerning tinted lenses, the practitioner should
cantly from all the other six tinted lenses at each question the patient not only on his sensitivity to
of the levels of illumination, it is evident that the light, but also as to the activities and levels of il-
tinted lenses adversely affected stereopsis at near lumination for which the lenses are to be used27.
under reduced levels of illumination. Pitts and Chou3 advised that care must be tak-
As it is necessary to constantly distinguish ob- en to ensure that the tint does not impair vision
jects in the environment at varying distances, any if industrial workers move quickly or frequently
from brilliant sunlight into a dimly lighted envi- pia, accommodation, retinal illuminance, and pupil size on stere-
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