Custom Practice Test - 20-Jun Contact Number: 8527521718

5. Which of the following correctly describes the

Biology naming convention for restriction enzymes?
1. Sticky ends produced by certain restriction The first letter represents the genus, the next
endonucleases are so named because: two letters represent the species, the strain is
1.
they form hydrogen bonds with their indicated by an additional letter, and Roman
1. numerals indicate the order of discovery.
complementary cut counterparts
they allow a host cell to retain a large number of The first letter represents the genus, the next
2. 2. two letters represent the species, and Roman
copies of vector DNA
they act as selectable markers to isolate numerals indicate the recognition sequence.
3. The first three letters represent the bacterial
transformed cells
they form covalent phosphodiester bonds with 3. species, followed by the strain name and Roman
4. numerals indicating the recognition sequence.
their complementary cut counterparts
The entire name of the bacterial strain is used,
4. followed by Roman numerals indicating the
2. Match List-I with List-II enzyme's function.
List-I List-II
A. Ampicillin I. Restriction sites
6. The balance and concentration of which two of
B. Ori II. Blue/White screening
the given plant growth regulators are most crucial
Multiple cloning and often need to be optimized for organogenesis
C. III. Selectable marker
sites from callus in plant tissue culture?
β galactosidase Sequence from where 1. Auxins and Cytokinins
D. IV.
gene replication starts 2. Auxins and Gibberellins
Choose the correct answer from the options given 3. ABA and Ethylene
below: 4. Ethylene and Auxins
1. A-III, B-IV, C-I , D-II
2. A-III, B-I, C-IV, D-II 7. Match each item in Column I with one in Column
3. A-IV, B-III, C-II , D-I II and select the correct match from the codes
4. A-II, B-IV, C-I, D-III given:
Column I Column II
3. The first clinical gene therapy was given in 1990 Plasmid of E.coli
to correct: A ADA deficiency P
used as vector
1. Sickle cell anaemia 2. ADA deficiency Production of human Human protein
3. Phenylketonuria 4. Burkitt’s lymphoma B Q
insulin enriched milk
C Transgenic cow R Retroviral vector
D Transgenic sheep S Alpha-1-antitrypsin
4. What is an advantage of recombinant insulin
Codes:
over insulin obtained from animal sources?
A B C D
Recombinant insulin is cheaper to produce and
1. 1. P R Q S
more ethical.
It is identical to human insulin, reducing allergic 2. R P S Q
2. 3. P R S Q
reactions and increasing efficacy.
3. It can be orally ingested instead of injected. 4. R P Q S
4. It has a longer shelf-life.

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8. Which one is a true statement regarding DNA 11. Restriction enzymes produced by some
polymerase used in PCR? bacteria:
It is used to ligate introduces DNA in recipient cleave phosphodiester bond in the main
1. 1.
cells chromosomal DNA of the bacterium.
2. It serves as a selectable marker 2. cut single stranded DNA at random positions.
3. It is isolated from a virus prevent the multiplication of bacteriophage in
3.
4. It remains active at high temperatures bacteria.
4. provide resistance against antibiotics.

9. Given below are two statements: One is labelled

as Assertion (A) and the other is labelled as 12. Which peptide, present in the pro-insulin, is
Reason (R) absent from the final structure of insulin?
Restriction enzyme is a type of 1. The A peptide
Assertion (A):
endonuclease 2. The B peptide
Restriction enzyme cuts the two 3. The C peptide
Reason (R): stands of DNA at specific positions 4. The A and C peptides
within the DNA
13. The Ti Plasmid [ having T-DNA] is considered a
natural genetic engineer and can transform:
In the light of the above statements, choose the 1. bacterial cells
correct answer from the options given below: 2. dicot plant cells
3. monocot plant cells
1. (A) is True but (R) is False. 4. animal cells
2. (A) is False but (R) is True.
14. Consider the given two statements:
Both (A) and (R) are True and (R) is the correct
3. It is desirable to use restriction
explanation of (A).
enzymes that make asymmetrical
Both (A) and (R) are True but (R) is not the Assertion (A):
4. cuts in construction of rDNA
correct explanation of (A).
molecules.
Formation of asymmetrical cuts
Reason (R): facilitates the function of DNA
10. Given below are two statements, one is labelled
ligase.
as Assertion (A) and the other is labelled as
Reason (R).
When a particular restriction Both (A) and (R) are True and (R) correctly
1.
enzyme cuts strands of DNA, explains (A).
Assertion (A): Both (A) and (R) are True but (R) does not
overhanging stretches or sticky 2.
ends are formed. correctly explain (A).
Some restriction enzymes cut the 3. Both (A) and (R) are False.
Reason (R): strand of DNA a little away from the 4. (A) is True, (R) is False.
centre of palindromic site.
In light of the above statements, choose the correct
answer from the options given below:
Both (A) and (R) are True and (R) correctly
1.
explains (A).
Both (A) and (R) are True and (R) does not
2.
correctly explain (A).
3. (A) is True, (R) is False.
4. (A) is False, (R) is True.

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15. Why is the plasmid vector used in recombinant 18. Which of the following describes the correct
DNA technology capable of replicating function of a probe in molecular biology?
independently within a host cell? A double-stranded DNA molecule that directly
Because plasmids contain antibiotic resistance 1. binds to proteins in order to regulate gene
1.
genes that trigger replication. expression.
Because plasmids are naturally resistant to An enzyme that cleaves specific DNA
2. restriction enzymes, making replication 2. sequences for identification in gel
possible. electrophoresis.
Because plasmids possess an origin of A protein molecule used to amplify genetic
3. replication that allows them to replicate material during polymerase chain reaction (PCR)
3.
autonomously. allowing its identification even if present in
Because plasmids are self-sustaining entities small amounts in the original sample.
4.
that can exist outside the nucleus. A single-stranded DNA or RNA molecule tagged
with a radioactive marker that hybridizes with its
4.
complementary DNA sequence, followed by
16. Consider the given two statements: detection using autoradiography.
Transgenic mice are being
developed for use in testing the
Assertion (A):
safety of oral polio vaccines before 19. Biological theft, illegal collection of indigenous
they are used on humans plants by corporations who patent them for their
Mice are immune to infection by the own use, comes under:
Reason (R): 1. Bioprospecting 2. Bioremediation
polio virus.
3. Biopiracy 4. Bioethics
Both (A) and (R) are True and (R) correctly
1.
explains (A).
Both (A) and (R) are True but (R) does not 20. What is true about RNA interference?
2. 1. It enhances the expression of specific genes.
correctly explain (A).
3. (A) is True; (R) is False It is a process that silences gene expression
2.
4. Both (A) and (R) are False using double-stranded RNA molecules.
It primarily involves altering the DNA sequence
3.
to block gene expression.
17. What was the key contribution of Cohen and 4. It is used to introduce new traits into organisms.
Boyer to biotechnology?
1. They discovered the structure of DNA.
2. They developed the first recombinant DNA. 21. Select the restriction endonuclease enzymes
whose restriction sites are present for the
They were the first to sequence the human
3. tetracycline resistance (tetR) gene in the pBR322
genome.
cloning vector.
They discovered how to visualize DNA
4. 1. Bam HI and Sal I
fragments using agarose gel electrophoresis
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI

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22. Consider the given two statements: 25. A foreign DNA and plasmid cut by the same
Enzyme replacement therapy for restriction endonuclease can be joined to form a
Assertion (A): ADA deficiency is not a completely recombinant plasmid using?
curative treatment. 1. Eco Rl 2. taq polymerase
Enzyme replacement therapy 3. polymerase lll 4. ligase
provides functional ADA to the
Reason (R): patient but does not correct the
genetic defect that causes the 26. The first gene therapy trial was done to treat:
deficiency. 1. Emphysema
2. ADA deficiency
Both (A) and (R) are True and (R) is the correct 3. Sickle cell anaemia
1. 4. Phenylketonuria
explanation of (A).
Both (A) and (R) are True but (R) is not the
2. 27. Why was the transgenic cow Rosie considered
correct explanation of (A).
significant in biotechnology?
3. (A) is True but (R) is False.
It produced genetically modified cow milk with a
4. (A) is False but (R) is True. 1.
higher fat content for better commercial use.
It produced milk enriched with human alpha-
2. lactalbumin, making it more suitable for human
23. Identify the wrong statement with regards to
infants.
restriction enzyme:
1. They cut the strength at palindromic sites. It was the first cloned animal that could produce
3.
medicinal proteins in its milk.
2. They are useful in genetic engineering.
It was developed using CRISPR technology to
3. Sticky ends can be joined by using DNA ligases.
4. enhance milk yield and improve resistance to
Each restriction enzyme functions by inspecting diseases.
4.
the length of a DNA sequence.

24. Given below are two statements : one is

labelled as Assertion (A) and the other is labelled
as Reason (R).
Agar gel electrophoresis is
Assertion (A): employed to check the progression
of a restriction enzyme digestion.
Restriction enzyme digestion is
performed by incubating purified
Reason (R): DNA molecules with the restriction
enzymes, at the optimal conditions
for that specific enzyme.
In the light of the above statements, choose the
most appropriate answer from the options given
below :
1. Both (A) and (R) are False.
Both (A) and (R) are True but (R) does not
2.
explain (A).
3. (A) is True but (R) is False.
4. Both (A) and (R) are True and (R) explains (A).

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28. What is true regarding A, B and C in the given 32. Which of the following statements about the
diagrammatic representation of rDNA technology? origin of replication (ori) in a cloning vector is
correct?
It controls the expression of the antibiotic
1.
resistance gene.
It is the sequence where replication starts and
2. also determines the copy number of the linked
DNA.
It is the recognition site for restriction
3.
endonucleases used in cloning.
It facilitates the process of ligation of foreign
4.
DNA into the vector.

33. The most important feature in a plasmid to

serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
At A, same restriction enzyme is used to cut 2. Presence of a selectable marker
I: 3. Presence of sites for restriction endonuclease
both foreign and vector DNA
4. Its size
II: The enzyme used at B is DNA ligase
III: Step C can be called as transformation 34. Consider the given two statements:
Insulin isolated from other cattle or
1. I and II only 2. I and III only Assertion(A): pigs is not as effective as that
3. II and III only 4. I, II and III secreted by the human body itself.
Insulin isolated from other cattle or
Reason (R): pigs will elicit an immune response
29. Which of the following can be used as gene in the human body
probes in molecular biology?
1. A dsRNA Both (A) and (R) are True and (R) correctly
2. A dsDNA 1.
explains (A).
3. Either ssRNA or ssDNA
Both (A) and (R) are True but (R) does not
4. Can be ssDNA but not ssRNA 2.
correctly explain (A).
30. Root knot of tobacco is caused due to an 3. (A) is True; (R) is False
infection by: 4. Both (A) and (R) are False
1. Radopholus similis
2. Meloidegyne incognitia
3. Globodera rostochiensis 35. α-1 antitrypsin is
4. Ditylenchus dipsaci 1. an antacid
2. an enzyme
31. It is possible to raise a large number of 3. used to treat arthritis
plantlets through micro-propagation because of the 4. used to treat emphysema
presence of which of the following properties in
plants?
1. Cellular totipotency
2. Phenotypic plasticity
3. Closed development
4. Determined growth

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36. A plasmid has genes for resistance against 40. In the given figure of pBR322, A, B and C are
ampicillin and tetracycline. The tetracycline respectively the recognition sequences for:
resistant gene is insertionally inactivated by
inserting a foreign DNA within the gene. The
bacterial cells are transformed. The recombinant
transformants:
will be killed in a medium containing ampicillin
1.
but not in a medium containing tetracycline
will be killed in a medium containing tetracycline
2.
but not in a medium containing ampicillin
will be killed in a medium containing ampicillin
3.
and in a medium containing tetracycline
will not be killed in a medium containing
4. ampicillin and in a medium containing
tetracycline A B C
1. Pst I EcoR I BamH I
2. Pst II EcoR I BamH I
37. Eli Lilly prepared two DNA sequences
3. Pst I Hind II BamH I
corresponding to A and B chains of human insulin
and introduced them in: 4. Pst I EcoR I Hind III
1. plasmids of E. coli to produce insulin chains.
2. pBR322 plasmids to produce insulin chains.
41.
3. pUC101 plasmids to produce insulin chains. Repeated amplification of DNA in
Ti plasmids of Agrobacterium to produce insulin Polymerase Chain Reaction is
4. Assertion (A):
chains. achieved by the use of a
thermostable DNA polymerase.
Such DNA polymerase remains
38. The trigger for activation of toxin of Bacillus active during the high temperature-
thuringiensis is: Reason (R): induced denaturation of double-
1. Acidic pH of stomach stranded DNA, a step in Polymerase
2. High temperature Chain Reaction.
3. Alkaline pH of gut
4. Mechanical action in the insect gut Both (A) and (R) are True and (R) is the correct
1.
explanation of (A).
Both (A) and (R) are True but (R) is not the
2.
39. Consider the given two statements: correct explanation of (A).
RNA interference (RNAi) is a 3. (A) is True but (R) is False.
Statement I: biological process that regulates 4. (A) is False and(R) is True.
gene expression.
RNAi takes place only in plants as a
Statement II:
method of cellular defense.

1. Statement I is correct; Statement II is correct

2. Statement I is incorrect; Statement II is correct
3. Statement I is correct; Statement II is incorrect
Statement I is incorrect; Statement II is
4.
incorrect

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42. Consider the given two statements: 45. Human insulin produced through recombinant
Bt cotton contains genes from the DNA technology:
bacterium Bacillus thuringiensis, Has a higher risk of causing allergies compared
Statement I: 1.
which produce Cry proteins toxic to to pig insulin.
specific insect pests. Is exactly the same as insulin produced in the
2.
The Cry proteins formed in Bt cotton human pancreas.
dissolve the gut lining of insects, Is produced by inserting the human insulin gene
Statement II: 3.
leading to their death when they feed into bacteria.
on the cotton leaves. 4. Requires human cells for production.
1. Statement I is True, Statement II is False.
2. Statement I is False, Statement II is True.
3. Both Statement I and Statement II are True. 46. Consider the two statements:
4. Both Statement I and Statement II are False.
The proteins encoded by the genes
Statement I: cryIAc and cryIIAb control the corn
43. Identify the correct statements:
borer.
ADA deficiency is caused due to the deletion of
I: The proteins encoded by the gene
the gene for adenosine deaminase. Statement II:
cryIAb control the cotton bollworms.
Bone marrow transplant and enzyme
II:
replacement therapy are completely curative.
1. Statement I is correct; Statement II is correct
Lymphocytes transformed with cADA gene are
III. 2. Statement I is incorrect; Statement II is correct
permanent cure for the condition.
3. Statement I is correct; Statement II is incorrect
1. Only I and II 2. Only I Statement I is incorrect; Statement II is
4.
incorrect
3. Only II and III 4. I, II and III

47. The restriction endonuclease enzymes have

44. Consider the given two statements:
been isolated from:
In order to force bacteria to take up
1. Bacteria 2. Yeasts
the plasmid, the bacterial cells must
Assertion (A): 3. Algae 4. Fungi
first be made ‘competent’ to take up
DNA.
All bacterial cells already have
48. The requirement of primers in a polymerase
Reason (R): plasmids and will not normally pick
up another. chain reaction (PCR) is because they:
1. Provide the starting point for DNA synthesis.
Both (A) and (R) are True and (R) correctly 2. Help in cutting the DNA into smaller fragments.
1. 3. Join the DNA fragments after amplification.
explains the (A).
2. (A) is True; (R) is False 4. Act as selectable markers.
3. (A) is False; (R) is False
Both (A) and (R) are True but (R) does not
4. 49. A single-stranded nucleic acid fragment, tagged
correctly explain the (A)
with a radioactive molecule, that interacts with a
complementary sequence of a target nucleic acid
is called a:
1. Gene gun 2. Gene probe
3. Snip 4. Selectable marker

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50. In a continuous culture system: 54. In recombinant DNA technology antibiotics are
The used medium is drained out from one side used:
I:
while the fresh medium is added from the other. 1. to keep medium bacteria-free
The cells are maintained in their physiologically 2. to detect alien DNA
II: 3. to impart disease-resistance to the host plant
most active log/exponential phase.
4. as selectable markers
A larger biomass is produced leading to higher
III:
yields of desired proteins.
55. Consider the following statements:
RNAi takes place in all eukaryotic organisms
1. Only I and II are correct I:
as a method of cellular defense.
2. Only I and III are correct
It involves the silencing of a specific mRNA
3. Only II and III are correct II: due to complementary dsDNA molecules that
4. I, II and III are correct prevent the translation of mRNA.
Fire and Mello got the Nobel Prize for the
III:
discovery of RNAi.
51. Which of the following best defines
downstream processing in biotechnology? Which of the above statements are true?
The genetic modification of cells to optimize
1. protein expression in recombinant DNA 1. I and II only 2. I and III only
technology. 3. II and III only 4. I, II, and III
The purification, separation, and formulation of
2.
a biotechnological product after biosynthesis.
The initial culturing and amplification of 56. The first transgenic cow produced:
3. bacterial or fungal cells to produce desired 1. enzyme to treat emphysema
biomolecules. 2. human protein enriched milk
The breakdown of unwanted by-products 3. enzyme to treat phenylketonuria
4. generated during fermentation in microbial 4. organs for xenotransplantation
processing.

57. Which of the following statements about

52. What is the criterion for the movement of DNA restriction enzymes is incorrect?
fragments on agarose gel during gel Restriction enzymes are also called molecular
electrophoresis? 1. scissors because they cleave DNA at specific
The smaller the fragment size, the farther it sequences.
1.
moves
They are naturally found in eukaryotic cells to
Positively charged fragments move to farther 2.
2. protect against viral DNA.
end
EcoRI is a restriction enzyme that recognizes the
3. Negatively charged fragments do not move 3.
sequence GAATTC.
The larger the fragment size, the farther it Restriction enzymes often create sticky ends,
4. 4.
moves facilitating ligation.

53. The committee responsible for the appraisal of 58. The process of separation and purification of
activities involving large-scale use of hazardous expressed protein before marketing is called:
microorganisms and recombinants in research and 1. Downstream processing
industrial production from the environmental angle 2. Bioprocessing
is the: 3. Post-product processing
1. Genetic Engineering Appraisal Committee 4. Upstream processing
2. Gene Technology Approval Committee
3. Department of Biotechnology [DBT]
4. Indian Council of Agricultural Research

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59. What is the term used to describe the series of 62. Why is insertional inactivation an effective
processes, including separation, purification, method for identifying recombinant colonies?
formulation, and quality control, that a product It prevents the replication of plasmids without
undergoes after the biosynthetic stage? 1.
inserts in the host cells.
1. Upstream processing It confers antibiotic resistance to recombinant
2. Downstream processing 2. colonies while non-recombinants remain
3. Quality assurance sensitive.
4. Clinical trials
It allows for differentiation of recombinant
3. colonies based on their inability to produce a
60. Consider the given two statements:
specific enzyme.
The milk produced by the first
It facilitates the formation of complementary
transgenic cow, Rosie, was 4.
sticky ends for ligation.
Assertion (A): nutritionally a more
balanced product for human babies
than natural cow-milk.
63. The production of recombinant human insulin
The first transgenic cow, Rosie,
was a major breakthrough in biotechnology. How
Reason (R): produced human lactose-enriched
was the issue of producing functional insulin
milk.
solved using genetically engineered bacteria?
Proinsulin was directly inserted into E. coli,
Both (A) and (R) are True and (R) correctly 1. which automatically converted it into active
1.
explains (A). insulin.
Both (A) and (R) are True but (R) does not Insulin genes were inserted into plant cells,
2. 2.
correctly explain (A). which produced fully functional insulin.
3. (A) is True; (R) is False A single bacterial cell produced both chains of
4. Both (A) and (R) are False 3. insulin simultaneously, eliminating the need for
post-translational modifications.
The A-chain and B-chain of insulin were
61. Consider the given two statements: 4. synthesized separately and later joined by
A piece of DNA, if needed to be disulfide bonds.
Assertion (A): propagated during recombinant
DNA procedures, requires a vector.
The vectors provide the origin of 64. Identify the correct statement:
Reason (R):
replication. Agrobacterium tumefaciens is able to transform
1.
large number of monocot plants.
Both (A) and (R) are True but (R) does not Micro-injection is the only way to transform
1. 2.
correctly explain (A). animal cells.
2. (A) is False but (R) is True. Competence in bacterial cells can be induced by
3.
3. (A) is True but (R) is False. trivalent anions like phosphates.
Both (A) and (R) are True and (R) correctly Biolistics or gene gun is a suitable method to
4. 4.
explains (A). transform plant cells.

65. The first restriction endonuclease, whose

functioning depended on a specific DNA nucleotide
sequence was:
1. EcoR I 2. BamH I
3. Hind II 4. Sma I

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66. Retroviruses: 70. ELISA stands for:

I: contain ssRNA as their genetic material. 1. Easily Located Immuno Specific Antigen
have the presence of reverse transcriptase, an 2. Early Live Inactive Surface Antigen
II:
enzyme that transcribes RNA into DNA. 3. Energy Loaded Intermediate Site on Apoenzyme
1. Only I is correct 4. Enzyme-Linked Immuno Sorbent Assay
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect 71. To produce recombinant human insulin, the
pharmaceutical company Eli Lily:
67.
produced chains A and B together, cleaved them
It is possible to produce a human 1.
and combined them by disulfide bonds
Assertion(A): protein inside a bacteria, using
produced chains A and B separately, extracted
recombinant DNA technology. 2.
and combined by creating disulfide bonds
Nucleic acid is the genetic material
Reason (R): produced chains A and B together, cleaved them
of all organisms without exception. 3.
and combined them by hydrogen bonds
produced chains A and B separately, extracted
1. (A) is True but (R) is False. 4.
and combined by creating hydrogen bonds
Both (A) and (R) are True and (R) is the correct
2.
explanation of (A).
3. (A) is False and (R) is True. 72. Consider the given two statements:
Both (A) and (R) are True but (R) is not the A selectable marker in a plasmid is
4.
correct explanation of (A). Assertion (A): necessary for identifying
recombinant bacteria.
Selectable markers help in the
68. A single strand of nucleic acid tagged with a Reason (R): replication of plasmids inside the
radioactive molecule is called: host bacteria.
1. Vector
2. Selectable marker
Both (A) and (R) are True and (R) is the correct
3. Plasmid 1.
explanation of (A).
4. Probe
Both (A) and (R) are True but (R) is not the
2.
69. Match each item in Column I with one in
correct explanation of (A).
Column II and select the correct match from the 3. (A) is True but (R) is False.
codes given: 4. Both (A) and (R) are False.
Column I Column II
A PCR P E.coli plasmid
73. Consider the given two statements:
B ELISA Q DNA amplification
Bt cotton plants are resistant to
C Human insulin R Retrovirus Assertion (A):
insect pests like the bollworm.
Antigen-antibody
D Gene therapy S The Bt toxin produced in these
interaction
Reason (R): plants is harmless to mammals and
Codes:
to bacillus itself.
A B C D
Choose the correct option.
1. Q S R P Both (A) and (R) are True and (R) is the correct
2. Q S P R 1.
explanation of (A).
3. S Q P R Both (A) and (R) are True but (R) is not the
2.
4. S Q R P correct explanation of (A).
3. (A) is True but (R) is False.
4. (A) is False but (R) is True.

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74. Separation of DNA, fragments is done by a 79. The biggest advantage of using Recombinant
technique known as : DNA technology, PCR and ELISA, over conventional
1. Polymerase Chain Reaction diagnostic techniques like serum and urine analysis
2. Recombinant technology will be:
3. Southern blotting 1. cost effectiveness
4. Gel electrophoresis 2. early detection
3. absence of adverse effects
75. Which of the following base sequences is a 4. universal availability
part of the recognition sequence of EcoR I?
1. AGGAGG 80. If you want to generate virus-free plant through
2. TATAAT micropropagation [plant tissue culture], the explant
3. GAATTC you should use is:
4. TTTAAA 1. Meristem
2. Embryo
76. How does the bacterium protects its own DNA 3. Anther
from the action of restriction endonucleases 4. Ovule
present in its cytoplasm?
its DNA lacks active sites for the restriction 81. In Bt cotton, the Bt toxin present in plant tissue
1. as pro-toxin is converted into active toxin due to:
endonucleases
2. its DNA is protected within a nuclear envelope 1. alkaline pH of the insect gut
2. acidic pH of the insect gut
it adds methyl groups to the active sites of
3. 3. action of gut microorganisms
restriction endonucleases on its DNA
4. presence of conversion factors in the insect gut
the DNA gets heterochromatised to prevent the
4.
action of restriction enzymes
82. Consider the two statements:
Most of the developing and underdeveloped
77. A plasmid has a single recognition sequence
I: world is rich financially, but poor in biodiversity
for EcoRI. When cut with this enzyme, the number and traditional knowledge.
of resultant fragments will be:
Most of the industrialised nations are rich in
1. 1
II: biodiversity and traditional knowledge related to
2. 2
bio-resources but poor in biotechnology.
3. 3
4. 4
1. Statement I is correct.
78. Agrobacterium tumefaciens is widely used in 2. Statement II is correct.
plant genetic engineering because: 3. Both Statement I and Statement II are correct.
It naturally transfers the T-DNA region from its Ti 4. Both Statement I and Statement II are incorrect.
1.
plasmid into the plant genome.
It produces restriction enzymes that facilitate
2.
foreign DNA insertion into plants. 83. Why are the sticky ends produced by restriction
It generates transgenic plants by transferring enzymes important in recombinant DNA
3.
viral DNA into host cells. technology?
It directly injects foreign proteins into plant cells They protect the DNA fragments from
4. 1.
for transformation. exonucleases.
They prevent the insertion of DNA fragments
2.
into the vector.
They ensure that the DNA fragments cannot be
3.
re-ligated by DNA ligase.
They allow fragments with identical sticky ends
4.
to hydrogen bond and facilitate ligation.

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 Custom Practice Test - 20-Jun Contact Number: 8527521718

84. Which of the following best explains why PCR 89. ELISA works on the principle of:
is a powerful tool for early disease diagnosis? 1. Anamnestic response
PCR can detect the symptoms of a disease even 2. Antigen-antibody interaction
1. 3. Complementary probe hybridization
in the absence of a pathogen.
PCR amplifies even small quantities of nucleic 4. Culture of microbe in a nutrient medium
2. acids, allowing detection of pathogens at very
low concentrations. 90. In the vector pBR322, you can ligate a foreign
DNA at the:
PCR uses enzyme-linked antibodies to detect
3. BamH I site of tetracycline resistance gene in
pathogen proteins in a sample. 1.
the vector pBR32
PCR requires no prior knowledge of the genetic
4. Sal I site of ampicillin resistance gene in the
sequence of the pathogen. 2.
vector pBR322.
Pst I site of tetracycline resistance gene in the
3.
85. The process by which a virus transfers genetic vector pBR322.
material from one bacterium to another is called: EcoR I site of ampicillin resistance gene in the
4.
1. Transduction 2. Conjugation vector pBR322
3. Transformation 4. Translation

86. The first bioinsecticide is generally considered Fill OMR Sheet*

to be:
1. Bacillus thuringiensis *If above link doesn't work, please go to test link from
2. Agrobacterium tumefaciens where you got the pdf and fill OMR from there
3. Paecilomyces fumosoroseus
4. Trichoderma harzianum

87. Some strains of Bacillus thuringiensis produce

proteins that kill certain insects such as:
I:
lepidopterans including tobacco budworm and CLICK HERE to get
armyworm
II: coleopterans including beetles
FREE ACCESS for 2
III: dipterans including flies and mosquitoes days of ANY NEETprep
1. Only I and II are correct
2. Only I and III are correct course
3. Only II and III are correct
4. I, II and III are correct

88. Even if a plant is infected with a virus:

I: the apical meristem is free of virus
II: the axillary meristem is free of virus
meristem can be grown in-vitro to obtain virus
III:
free plants

Only I and III are Only II and III are

1. 2.
correct correct
Only I and II are
3. 4. I, II and III are correct
correct

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