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                               1
                                Ministry of Agriculture and Natural Resoureces, Gezira State Sudan
                      2
                       College of Agricultural Studies ,Shambat .Sudan University of Science and Technology
                                  *Corresponding Author: izzeldienadammohmmed@gmail.com
Abstract Field experiments were conducted in the                     Maize grain enters also in livestock feeds, and in other
Demonstration Farm of Mohammed Osman Salih ( Blue Nile            industrial purposes as in the case of glucose and starch and
North of Gezira state / ElBagir area) for the two consecutive     edible oil industries.
seasons ( summer 2007 /2008 and 2008 /2009 ) to study the            In industrialized countries, maize is largely used as
effect of nitrogen and weeding levels on yield of five            livestock feed and as a raw material for industrial products,
cultivars of maize (Zea Mays L.) under irrigation. The            while in developing courtiers it is mainly used for human
treatments used consisted of three levels of nitrogen control     consumption. In sub-Saharan Africa, maize is a staple food
(N0) , 86 kg N/ha (N1), 129 kg N/ha (N2), two levels of           for 50% of the population. It is important source of
weeding non-weed control (W0) , weeding (W1) and five             carbohydrate, protein, vitamin B, and minerals. Green maize
cultivars, open pollinated Giza-2 (V1) , Var-113 (V2) ,           (fresh on the cob) is eaten boiled, playing an important role
Hudiba-1 (V3) and Hybrid cultivars, Hycorn-90 (V4) and            in filling the hunger gap CIMMYT[1]
Hycorn-675 (V5).The 30 factorial treatments were executed            Maize (Zea mays L,) is grown on approximately 140
in randomized complete block design, with four replications.      million hectares (M/ha) worldwide: 97 M/ha in developing
The results obtained showed that nitrogen fertilizer had a        countries, 34 M/ha in the high income countries, and 9 M/ha
significant effect on final seed yield and some yield             in the Eastern Europe and the former Soviet Union
components as cob yield and number of seeds per cob when          http://www.c[2]
different nitrogen doing with no-weeding was not significant         Maize is a staple food for several hundred million people
for seed yield. The results from these experiments showed         in the developing world. The average inhabitant of Eastern
that hybrid cultivars maize efficiently utilized nitrogen         and Southern Africa consumes about 80 kg of maize each
without weeding better than the open cultivars except             year; while in Mexico, Central America, and the Caribbean
Giza-2.The results obtained showed negligible differences         he consumes about 170 kg. Annual per capita maize
between the two seasons for yield . This justifies growing        utilization averages 100kg in East Asia and more than 190 kg
maize in summer with 129 kg/ha nitrogen and good                  in the southern cone of South America, largely as animals
cultivation.                                                      feed in both cases. Unfortunately, developing countries do
                                                                  not produce enough maize to meet their needs and must
Keywords          Cultivar,   Irrigation,   Nitrogen,   Yield,    therefore import approximately 30 million tons of maize
Weeding.                                                          annually. Use of improved cultivars and management
                                                                  practices should help increase maize yield and reduce
                                                                  imports in developing countries. {(http:// www[3]
                                                                     Maize is the second most important crop of New World
1. Introduction                                                   food programme (Anonymous[4] In New World food
                                                                  programme, maize is grown on 539,215 ha with a total
   Maize or corn as a world crop is grown in various              production of 836,446 tons and per ha yield of 1551 kg. It
countries. The crop is widely used as a food crop in many         serves as a food, fodder and feed and also as a source of raw
parts of the world especially in the tropical and subtropical     material for the industry. Average yield of maize in Pakistan
countries. Maize is rich in starch (carbohydrates) with an        and New World food programme is very low as compared
average of about 70%, but low in protein (about 9.5%). The        to other maize growing countries of the world .
oil is concentrated mainly in the germ with an average of 4%         The weed control is an important management practice for
of the kernel weight. The compositions of the other               maize production and should be carried out to ensure
components of the kernel are 1.4% sugars, 2.3 crude fibre         optimum grain yield .Weed control in maize is carried out by
and 1.4% ash.                                                     mechanical and/ or chemical methods. Weed between plants
120                Effect of Different Nitrogen and Weeding Levels on Yield of Five Maize cultivars under Irrigation
rows are removed generally by mechanical cultivation.                  For the security reasons and the reliability of the result
While weeds on the rows are controlled by hand hoeing or by         they were conducted in the farms of Osman Salih ElBagir
herbicides, and both methods are effective in controlling           area (North of Gezira state. Sudan) latitude 15-22N and
weeds.                                                              longitude 32-46 E.
   The average annual harvested area in the world is over 150          Prior to commencement of the experiment, soil samples
million hectares with seed production of about 800 million          were taken for the analysis of soil, which showed that soil
metric tons with an average yield of 4970.9 kilogram per            was sandy clay loam with a pH range from 8.29-8.88. It was
hectares Wikipedia[5] The average harvested area in the             found that the sand content ranged from 38.80 to 69.80%, the
world is about 130 million hectares with seed production of         silt content from 4.44-28.00% and the clay contents from
about 455 million metric tons per hectare Ali[6] The United         24.48 to 33.20%. The nitrogen content was found to be
States exports about 59 per cent of the world trade of corn.        0.0079-8.035% and 0.0079-0.047% over dry weight. The
Other maize exporting countries include: China, Brazil,             phosphorus content 0.55-1.85 mg/L and the K contents was
Mexico, Argentina, India and France.                                0.200 to 0.92 mg/L .
   The leading importing countries include: Italy, Japan,              The climate of the locality is tropical semi-arid with low
United Kingdom, Germany, Netherlands and Spain                      relative humidity. During summer in the first season, the
Jugenheimer[7]. In Africa, the Portuguese brought maize             range of temperature was 41.6-19.5°C and during the second
from the West Indies to the island of Sao Tome, and from            season it was 42.6-19.2°C. Temperature, relative humidity
there it was taken to the Cold Coast. Apparently, it reached        and rainfall data for the two seasons was obtained from
South Africa shortly after the arrival of the first Dutch           Ministry of Sciences and Technology, Meteorological
colonists, where it now occupies 65% of the land under              Authority, Khartoum Station .
cropping, and it is the main staple food of the majority of the        The seeds of open-pollinated cultivars (Geza-2, Hudeiba-1
people. Another possibility is that the crop may have entered       and var-113) were obtained from the Seed Propagation
Africa through Egypt, where maize cultivation was                   Administration, Gezira Research Station. The 2 hybrid
established in the latter half of the eighteenth century with       cultivars (Hycorn-90 and Hycorn-675) were obtained from
cultivars imported from Syria and India Song et al[8].              Pacific Seeds PTY .LTD.
   In Sudan, Maize utilization as a human food for making              The experiment was factorial in a randomized complete
bread was limited, where most of the produce is consumed as         block design, with four replications. The treatments
roasted kernels or in mixing with wheat to make bread. The          consisted of the five cultivars of maize (V1 = Geza-2, V2 =
environmental conditions in the Sudan are generally                 var-113 , V3 = Hudiba -1,V4 = Hycorn-90, V5 = Hycorn-675.
favorable for maize production. Old statistics showed that          The three levels of nitrogen are:-
the crop used to be produced in various ecological zones                N0 =( control ) with no nitrogen , N1 ( 86kg N/ha) , N2
Arab[9] and AOAD[10].                                               (129 kg N/ha ): and the two levels of weed are : W0 ( control )
   The total area of maize was 160 hectares in 1999 and the         with no weed , W1 = weeding .
total production was 406 kg \ha Lower yields and low market            Before sowing the fertilizer was applied where nitrogen
prices, were the main factors, which gave low priority for          was broad-cast on top of the ridges .Sowing was done by
maize in agricultural development in the Sudan .Results             hand where 2 -3 seeds per hole were sown on top of the ridge
indicated the success of the crop under rain-fed conditions         in holes 20 cm within the ridge, then the seedings were
where an average yield of about 1.9 ton\feddan was obtained.        thinned to one plant per hole .The experiment was sown on
Further experimental trials were carried out by the Arab            the 27th of July in the first season (2007/2008) and on the 1st
African Company for Agricultural and Industrial                     of August (2008/2009).
Development under irrigation (3.000 feddans) in Gezira                 The watering interval was 8 -10 days in both season and
FAO[11].                                                            the plants were thinned to one plant per hole after seventeen
   As mentioned before, the yield of maize per unit area in         days from planting. More than three manual weedings in
the Sudan is the very low compared to the world average.            each seasons were carried out according to the block design
The reasons are many including management and the main              treatments .Termites infestation was observed when the crop
objective of this research is to study the effects of the           was 35 days old in the two seasons. The crop was sprayed
nitrogen and weeding on productivity of a number of maize           with Dursban 10ml/L in first season and in the second season
genotypes and to select the best cultivar for the prevailing        the crop sprayed with Morsban (480ml/L).
conditions.                                                            The number of cobs per plant was taken at 60 and 75 days
                                                                    from sowing, for each of the six plants per plot.
                                                                       The six plants of each plot were cut at 120 days from
2. Materials and Methods                                            sowing and sun-dried then weighed and the average dry
   The experiment was conducted for two consecutive                 weight per plant was recorded.
summer seasons of 2007/2008 and 2008/2009.                             When signs of maturity were clear on the plants (complete
   The objectives of this experimental were to investigate the      yellowing of leaves and cobs and partial shedding of leaves),
effect of nitrogen and weeding levels on yield of five maize        harvesting was made for the remaining plants for the plot to
(Zea mays) cultivars .                                              estimate the final yield.
                                              Universal Journal of Agricultural Research 1(4): 119-125, 2013                                                          121
   The cobs of six plants in each plot were measured and the                              3. Results
average cob length was recorded for each plot.
   The average number of seeds per cob and the average                                       Analysis of variance(table1,2) show clear significant (P=
weight per cob were also made.                                                            0.01) differences in dry weight between the levels of
   100-seeds were counted randomly and weighed for each                                   nitrogen. The application of 86 kg N/ha gave 20%,26%
plot.                                                                                     significantly higher dry weight over control, whereas the
   The cobs from each plot of the harvested plants were                                   application of 129 kg N/ha increased the dry weight per plant
weighed then threshed and the seed yields for each plot were                              over the control by 27%, 30% respectively. Also, in the two
recorded. Yield per hectare was then estimated.                                           season there were significant (P=0.01) differences between
   Data collected from the different treatment were subjected                             cultivars, where cultivar HyCorn-90 gave significantly
to analysis of variance (ANOVA) appropriate for                                           higher dry weight than the cultivars Giza-2, var.113,
randomized complete block design (Gomez and Gomez,                                        Hudiba-1 and Hycorn-675, which were significantly
1984).The means were separated using the least significant                                different from each other. The interaction between
difference (LSD) and Duncan Multiple Range Test (DMRT).                                   treatments and weeding were also significant .
Table 1. F-value of the measured variables for interaction of cultivar weeding and fertilizer treatment
                                                                               2007/08 season
                                                                                                                                      Length of
    Source of                      No .of            Dry weight/plant          Weight of          100-seeds             No .of                       Yield of seeds
                      d. f                                                                                                               cob
    variation                     rows/cob                  g                  seeds/cob            wt (g)            seeds/cob                          kg/ha
                                                                                                                                        (cm)
         V             4            6.68*               36.28**                 61.62**           16.55*              110.8**          35.18**        41.254**
         N             1          0.505NS               656.7**                 376.5**           267.1**             182.6**          94.05**        214.634**
                                                                                                  499.64*
        W              2          0.95NS                117.6**                  1724**                               61.30**          3.81NS          1.086NS
                                                                                                     *
       VN              4           5.38*                117.62**                56.92**            8.56*              6127**           4.99NS           39.55**
       VW              8           4.22**                27.98*                 76.62**           12.28**             54.72**          10.75*           48.48*
                                                                                                  113.25*
       NW              2          1.35NS                    20.84*               19.62*                               15.26**          3.59NS           10.352*
                                                                                                     *
     VNW               8           3.09*                98.44**                 64.57**            9.73*              29.84**          5.315*           43.55
    Ms. Error                      1.056                 43.93                   7.114             2.325              564.11            1.05            82.061
        SE                         0.297                 1.913                   0.770             0.440               7.383           0.296             2.3
       LSD                         0.6162                   4.195                1.693             0.9272              14.45            0.680            181.2
      C.V %                         1.5%                    0.7%                 7.5%              4.7%                8.3%             3.4%             15.6%
** = significant at1% level ( highly significant)
* = significant at 5% level (significant)
NS = not significant
Table 2. F-value of the measured variables for interaction of cultivars weeding and fertilizer treatments
   The application of 86 kg N/kg did not increase rows             whereas the application of 129kg N/ha increased the weight
number per cob compared to the control, whereas the                of seeds over the control by 106%, 84% respectively. On the
application of 129 kg N/ha increased the number of rows per        other hand, there were significant differences in the weight
cob over control by 8% in the first season, however                of seeds between the application of 86 and 129 kg N/ha in the
application of 86 and 129 kg N/ha gave similar significantly       both. Also, there were significant (P=0.01) differences
9% higher number of rows per cob over the control in the           between cultivars, in 2007/2008 Giza-2 gave significantly
second season. On other hand, there were no significant            higher weight of seeds than var-113, Hudiba-1, Hycorn-90
differences in the number of rows per cob between the              and Hycorn-675, which were not significantly different from
application of 86 and 129 kg N/ha (Table1,2). Also, there          each other. While in 2008/2009 Hycorn-90 and Hycorn -675
were no significant differences between the five cultivars in      gave significantly higher weight of seeds per cobs than
number of rows per cob. The differences between the two            Giza-2 ,var-113and         Hudiba -1, which were also
levels of weeding and the interaction were not significant in      significantly different from each other . The difference
the both.                                                          between the two levels of weeding and interaction were
   From the statistical analysis(table1,2), it was clear that      significant.
there were significant (P=0.01) differences in 100-seeds              From the statistical analysis (table1,2), it was clear that
weight between the levels of nitrogen. The application of 86       there were significant (P=0.01) differences in seeds number
kg N/ha gave 25% ,31% significantly higher 100-seeds               per cob between the levels of nitrogen. The application of 86
weight over the control, whereas the application of 129 kg         kg N/ha gave 40%,38% significantly higher number of seeds
N/ha increased the weight of seeds over the control by 69% ,       over the control, whereas the application of 129kg N/ha
75% respectively. First season there were significant (P=          increased the number of seeds per cob over the control by
0.01) differences between cultivars , where cultivars              27%,84% respectively. On the other hand, in the first season
Hycorn-90 and Hycorn-675 gave significantly higher                 there were no significant differences in the number of seeds
100-seeds weight than the cultivars Giza-2, var-113 and            per cob between the application of 86 and 129kg N/ha. Also,
Hudiba-1 which were not significantly different from each          there was significant (P=0.01) difference between cultivars,
other. In second season also There were significant (P=0.01)       where Giza-2 gave significantly higher number of seeds per
differences between cultivars, where Hudiba-1 gave                 cob than the cultivars var-113, Hudiba-1, Hycorn-90 and
significantly higher weight of 100-seed than Giza-2, var-113,      Hycorn-675.In the second season there were significant
Hycorn-90 and Hycorn-675, which were also significantly            differences in the number of seeds per cob between 86 and
different from each other. Weeding gave significant higher         129 kg N/ha. Also, there was significant (P=0.01) difference
seed weight over the control and treatment interaction were        between cultivars, where Hudiba-1 gave significantly higher
significant in both.                                               number of seeds per cob than Giza-2, var-113, Hycorn-90
   From the analysis of variance (table1,2), it was clear that     and Hycorn-675. Weeding gave significant increase in seed
there were significant differences in length of cob between        number over the control in both seasons.
the levels of nitrogen. The application of 129 kg N/ha gave           There were significant (P=0.01) differences in seed yield
8%,23% significantly longer cobs than the control                  per hectare between the levels of nitrogen. The application of
respectively, whereas in the first one the application of 86 kg    86 kg N/ha gave 49%, 29% significantly higher seed yield
N/ha did not increase the length of cobs over the control,         over the control, whereas the application of 129 kg N/ha
there were no significant differences in the length of cob         increased the seed yield over control by 106%, 86%
between the application of 86 and 129 kg N/ha, there were          respectively. On the other hand, there were significant
significant differences between the cultivars, where               differences in the seed yield between the application of 86
Hycorn-90, Hycorn-675 and Giza-2 gave significantly taller         and 129 kg N/ha (Table 1,2). Also in the first season there
length than var-113 and Hudeiba-1. While the second season .       were significant (P=0.01) differences between cultivars,
the application of 86 kg N/ha gave 15% significantly longer        whereas cultivar Giza-2 gave significantly higher seed yield
cobs than the control and there were significant differences       than var-113, Hudiba-1, Hycorn-90 and Hycorn-675, which
in the length of cobs between the application of 86 and 129        were significantly different from each other. season two
kg N/ha, there were significant differences between cultivars,     cultivar Hudiba-1 gave significantly higher seed yield than
where cultivars Hycorn-90 and Hycorn-675 gave                      the Giza-2, var-113, Hycorn-90 and Hycorn-675, which
significantly taller length than the cultivars Giza-2, var-113     were significantly different from each other.
and Hudeiba-1 which were not significantly from each other.           The weeding level gave significantly higher yield of seed
The differences between the two levels of weed, the weeding        kg/ha over control. Treatment interaction was also
levels gave 15%, 23% significant over the control                  significant.
respectively.
   Statistical analysis(table1,2) shows a significant effect of
nitrogen and weeding and their interaction an seed weight
                                                                   4. Discussion
per cob. The application of 86 kg/ha gave 44%, 38%                   Weeding had significant effect on dry weight and in
significantly heavier weight of seeds/cob over control,            general weed control gave higher dry weight in both seasons.
                                  Universal Journal of Agricultural Research 1(4): 119-125, 2013                              123
This result is confirmed by Teton-Kagho and Gardner[12].          obtained from plots kept weeded at early stage. Results from
Nitrogen application generally increased dry weight/plant         both seasons suggest that weed control should be carried out
and the increase reached significant levels at later stages of    for maize to provide maximum grain yield per cob. Results
growth in both seasons. Such effects could be attributed to       obtained in this study in both seasons showed that the
enhancement of vegetative growth and dry weight                   significant increase in seed yield and yield components were
production by nitrogen. The interaction of N with weeding         mainly due to the applications of nitrogen. The increase in
had significant effect on dry weight (at early stages at over     seed yield can be attributed to the increase in cob yield and
60 days from sowing) in the second season. It is well known       number of seeds per cob.
that the combined effect of nitrogen and weeding is greater          These increases were positively correlated with prolonged
than individual effect of each treatment by itself.               weed –free duration. However ,yields obtained from plots
   Effects of nitrogen on number of seeds per cob are             kept weed-free until the growth of maize were not significant
significant. Weed and the interaction of nitrogen were            from those plot kept weed-free from emergence until row
significant in both seasons. In this connection, Habib [13];      closure. This may be explained by the lower weed
Sharma[14]; Lucas[15] found significant effects of nitrogen       occurrence in maize until the 6-leaf stage. Hanway [35];
on number of seeds per cob. Singh et al[16] Gangwar and           Berzensy et al[36] and Delpine and Covareli [37] stated that
KaLm [17] and Cimmyt[18] found that maize yield and yield         the nutrient uptake of maize from soil is relatively small
components were significantly increased by application of         during the 2-4 leaf stage of development.
nitrogen.                                                            In this study, grain yield of Hybrids and open pollinated
   The result obtained in both seasons showed no significant      cultivars of maize was positively affected by nitrogen and
effect of nitrogen on 100-seeds weight, with significant          weeding significantly. In the first season, cultivar Giza-2
effect of weeding. Ali [19] and Ologunde and Ogunlela [20]        gave higher seed yield of 4.52t/ha, compared to other
found no significant effects of nitrogen on 100-seeds weight.     cultivars, but in the second season, cultivars Hycorn-90 and
Weed removal had no–significant effect on number of               Hycorn-675 (Hybrid) gave higher grain yield of 4.10t/ha.
rows/cob in both seasons, while weeding during different          Similar results were obtained by Adediran et al., [38]; FPDD
growth stages increased number of 100-seeds weight.               [39]. The yield of hybrid maize increased with increase in
Oleson et al.,[21] studied the response of corn to weeding        nitrogen rate.
and reported both negative and positive responses. Positive          From the comparison of the seed yield in the two seasons,
responses were associated with low weed growth and                it was clear that there were very little differences between
negative responses with high weed growth during maize             seasons, where in the first summer season seed yield varied
growth stages.                                                    between 2730 – 5631 kg/ha with an average of 4.18 t /ha
   The effects of nitrogen, weed and their interactions on        while the yield in the second season varied between 2.70
seeds yield were significant in both seasons. Sharma [22]         to5.03 t/ha with an average of 3.87 t/ha.
found that nitrogen had significant effect on weight of seeds
per plant. Gangwar and Kalm [23] stated that nitrogen
significantly influenced yield attributes. Also cultivars
                                                                  5. Conclusions
showed significant difference seed yield. These results are in       In this study, N application to maize tended to improve
agreement with the results of Singh and Saroha [24]; Karama       seed yeild. The response to N increased as level of N applied
Swamy [25] ; Richards et al [26] and Omara[27] . On the           increased. Thus it is feasible to recommend N application to
other hand, increasing nitrogen, irrespective of weeding,         maize under similar conditions .This suggest, as mentioned
gave a continuous increase in seeds yield. Similar results        earlier, that maize crop in this area can successfully be grown
were obtained by Ali [28] and Arab O [29]. Ebaisary et al.,       in the summer season in Northern, Gezira and similar areas
[30] found significant increases in seed yield with increasing    in Sudan.
levels of nitrogen but the increase in seed yield above the          The weed control used in this study failed to bring out
levels of 80 -130 kg N/ha was low and not significant. On the     clear differences in yield of maize. Also results of this
other hand, Abdel Malik et al[31]; Okajima et al[32] and          experiment showed that weeds cause 42-62% yield losses in
Affholder et al[33] found that seed yield increased               maize. However, a weed-free growth period during stages of
significantly with the application of nitrogen and weed           the crop was enough to prevent these losses under similar
control.                                                          growing conditions. These results are valuable for growers
   In the present study in the two seasons, nitrogen gave a       for developing an integrated weed control strategy.
consistently, significant increase in yield and yield                The experiment was conducted during summer in both
components. In this connection, Gardner et al[34] reported        seasons. Further studies are needed to compare summer
that various maize cultivars responded to the application of      growing with winter growing.
nitrogen up to 240 kg N/ha. The responses due to these high          The results from these experiments showed that hybrid
levels of nitrogen might be due to the poor soil conditions of    maize (Hycorn-90 and Hycorn-675) efficiently utilized
the experimental site or due to high response of the cultivars    nitrogen       better     than      the     open      pollinated
used. In this study the highest number of seed/cob was            (Hudiba-1andVar-113) with the exception of Giza-2.
124                  Effect of Different Nitrogen and Weeding Levels on Yield of Five Maize cultivars under Irrigation
[7]    Jugenheimer, R.W.(1976). Corn Improvement Seed                    [24] Gangwar, B. and Kalm, G.S. (1982). Intercropping of rainfed
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       On Many Factors. Pp. 11-23.Awiley Interscience Pub. Printed
                                                                         [25] Kamara swamy, R. (1973). A note on the availability of
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                                                                              phosphorous with advancement of crop growth. Madras
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       Proc.Natl.Acad.Sci. USA 100, 9055-9060 .[ PubMed] .
                                                                              Nitrogen fertilization of land continuously cropped to corn
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                                                                         [27] Omara, H.A. (1989). The effect of spacing, nitrogen and
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                                                                              phosphorous application on growth and yield of maize (zea
[10] Aoad-1986 Morocco Primary Feasibility Study For Corn                     mays L.). M.Sc Thesis, Fac. of Agric. University of Khartoum,
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[11] FAO, (1980). Improvement And Production Of Maize,                   [28] Ebaisory, E.M.; ElDin, M.M.B and Negm, M.A. (1980).
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                                                                              yield. Field Crop Abst., 37(2-3):115.
[12] Tetio-Kagho, F. And Gardner, F.P (1988). Response Of
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                                                                              Agric. Res. Rev. (Egypt). 52(4):57-61.
[13] Habib, M.M.; Badr, M.F. and Solliman, S.M. (1971). Yield
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     19:245-251.
                                                                         [31] Arab Organization For Agricultural Development
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[15]    Lucas, E.O (1986). The Effect Of Density And Nitrogen            [32] Okajima, H.; Taniyama, I.; Kawakami, R.And Fujitsu, I.
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[17] Gangwar, B. and Kalm, G.S. (1982). Intercropping of rainfed         [34] Gardner, C.A.C.; Bax, P.L.; Bailey, D.J.; Cavulieri, A.J.;
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