ASAT (GOT) FS (IFCC mod.

)
AST/SGOT Reagent Test Kit
Intended Use Reagent Preparation
Diagnostic reagent for in vitro quantitative determination of The reagents are ready to use.
ASAT/SGOT in human serum or plasma on photometric
analyzers.
Specimen
Item Code Pack Size Serum or heparin plasma
Stability [5]:
126019934840 R1: 4 x 60 mL; R2: 4 x 15 mL 4 days at 20 – 25°C
7 days at 4 – 8°C
Summary 3 at –20°C
Discard contaminated specimens. Only freeze once!
Alanine Aminotransferase (ALAT/ALT), formerly called Glutamic
Pyruvic Transaminase (GPT) and Aspartate Aminotransferase
(ASAT/AST), formerly called Glutamic Oxalacetic Transaminase Assay Procedure
(GOT) are the most important representatives of a group of Wavelength 340 nm
Optical path 1 cm
enzymes, the aminotransferases or transaminases, which
Temperature 37°C
catalyze the conversion of α-keto acids into amino acids by Measurement Against air
transfer of amino groups.
As a liver specific enzyme ALAT is only significantly elevated in
hepatobiliary diseases. Increased ASAT levels, however, can Sample/Calibrator 100 µL
occur in connection with damages of heart or skeletal muscle as Reagent 1 800 µL
well as of liver parenchyma. Parallel measurement of ALAT and Mix, incubate for 5 min., then add:
ASAT is, therefore, applied to distinguish liver from heart or Reagent 2 200 µL
skeletal muscle damages. The ASAT/ALAT ratio is used for Mix, read absorbance after 1 min. and start stopwatch.
differential diagnosis in liver diseases. While ratios < 1 indicate Read absorbance again 1, 2 and 3 min thereafter.
mild liver damage, ratios >1 are associated with severe, often
chronic liver diseases.
Calculation with factor
From absorbance readings calculate A/min and multiply by the
Method corresponding factor from table below:
Optimized UV-test according to IFCC (International Federation
of Clinical Chemistry and Laboratory Medicine) [modified]
A/min x factor = ASAT activity [U/L]
Principle 340 nm 1745
L-Aspartate + 2-Oxoglutarate < ASAT > L-Glutamate + Oxalacetate
With calibrator
Oxalacetate + NADH + H+ < MDH > L-Malate + NAD+ A / min Sample
ASAT [ U / L] x Conc . Calibrator [ U / L]
A / min Calibrator
Reagents
Reagent composition
R 1: TRIS Buffer 12.1 gm/L Conversion factor
Asparate 39g/L ASAT [U/L] x 0.0167 = ASAT [µkat/L]
LDH >1200U/L
Calibrators and Controls
R 2: 2-Keto glutaric acid 8.5 gm/L
NADH 1.1 gm/L For the calibration of automated photometric systems, DiaSys
TruCal U calibrator is recommended. This method has been
Preservatives & Stabilizers q.s.
standardized against the original IFCC formulation. For internal
quality control, DiaSys TruLab N and P controls should be
Storage Instructions and Reagent Stability assayed. Each laboratory should establish corrective action in
The reagents are stable up to the end of the indicated month of case of deviations in control recovery.
expiry, if stored at 2 – 8°C, protected from light and
contamination is avoided. Do not freeze the reagents! Performance Characteristics
Measuring range
Warnings and Precautions On automated systems the test is suitable for the determination
1. The reagents contain sodium azide (0.95 g/L) as of ASAT activities up to 500 U/L.
preservative. Do not swallow! Avoid contact with skin and In case of a manual procedure, the test is suitable for ASAT
mucous membranes. activities which correspond to a maximum of ΔA/min of 0.16 at
2. Reagent 1 contains animal material. Handle the product as 340 nm.If such values are exceeded the samples should be
potentially infectious according to universal precautions and diluted 1 + 9 with NaCl solution (9 g/L) and results multiplied by
good clinical laboratory practices. 10.
3. In very rare cases, samples of patients with gammopathy
might give falsified results [4].
Specificity/Interferences
4. Please refer to the safety data sheets and take the No interference was observed by ascorbic acid up to 30 mg/dL,
necessary precautions for the use of laboratory reagents. bilirubin up to 40 mg/dL and lipemia up to 2000 mg/dL
For diagnostic purposes, the results should always be triglycerides. The presence of hemoglobin in serum indicates
assessed with the patient’s medical history, clinical destruction of erythrocytes with release of ASAT, thus
examinations and other findings. producing high interference. For further information on
5. For professional use only! interfering substances refer to Young DS [6].
Traceability
The method has been standardized against the original IFCC
formulation.

Waste Management
Please refer to local legal requirements.

ASAT (GOT) FS - Page 1

Sensitivity/Limit of Detection 8. Lorentz K, Röhle G, Siekmann L. Einführung der neuen
The lower limit of detection is 2 U/L. Standardmethoden 1994 zur Bestimmung der katalytischen
Enzymkonzentrationen bei 37 °C. DG Klinische Chemie
Precision Mitteilungen 26; 1995; Heft 4.
Intra-assay precision Mean SD CV 9. Zawta B, Klein G, Bablok W. Temperature Conversion in
n = 20 [U/L] [U/L] [%] Clinical Enzymology? Klin. Lab. 1994; 40: 33-42.
Sample 1 35.15 0.55 1.57
Sample 2 41.81 0.52 1.24
Sample 3 179.43 1.46 0.81

Inter-assay precision Mean SD CV

n = 20 [U/L] [U/L] [%]
Sample 1 39 0.21 0.53
Sample 2 157 0.95 0.61
Sample 3 193 0.94 0.49

Method Comparison
A comparison of DiaSys ASAT (GOT) Reagent without P-5-P (y)
and a commercially available test (x) using 51 samples gave
following results:
y = 0.997 x + 0.621 U/L; r = 1.000

Reference Range
Women < 31 U/L < 0.52 µkat/L
Men < 35 U/L < 0.58 µkat/L

Each laboratory should check if the reference ranges are

transferable to its own patient population and determine own
reference ranges if necessary.

Limitations
Eventual GOT(AST), mod. IFCC carry-over to reagents Carbon
Dioxide (CO2) PEP-C and Protein Total in Urine/CSF
(Pyrogallol red). The actual carry-over depends on the
analyzer.

DiaSys Diagnostics India Private Limited

Clinical Interpretation
Plot No. A – 821, T.T.C. Industrial Area, MIDC,
The normal range of an SGOT test is generally between 8 and
Mahape, Navi Mumbai – 400710.
35 units per liter of serum. In general, men may naturally have
Maharashtra, India.
higher amounts of AST in the blood. A result above 35 for men
and 31 for women is high and may indicate damage.
Customer Care
For feedback/queries contact customer care at :
Literature Toll Free number : 1800 120 1447
1. Thomas L. Alanine aminotransferase (ALT), Aspartate Email ID : helpdesk.service@diasys.in
aminotransferase (AST). In: Thomas L, editor. Clinical Website : www.diasys.in
Laboratory Diagnostics. 1st ed. Frankfurt: TH-Books
Verlagsgesellschaft; 1998. p. 55-65. Revision No. :01
2. Moss DW, Henderson AR. Clinical enzymology. In: Burtis CA, Mar. 2022
Ashwood ER, editors. Tietz Textbook of Clinical Chemistry.
3rd ed. Philadelphia: W.B Saunders Company; 1999. p. 617-
721.
3. Bergmeyer HU, Horder M, Rej R. Approved Recommendation
(1985) on IFCC Methods for the Measurement of Catalytic
Concentration of Enzymes. L.Clin. Chem. Clin. Biochem 1986;
24: 497-510.
4. Bakker AJ, Mücke M. Gammopathy interference in clinical
chemistry assays: mechanisms, detection and prevention.
ClinChemLabMed 2007;45(9):1240-1243.
5. Guder WG, Zawta B et al. The Quality of Diagnostic Samples.
1st ed. Darmstadt: GIT Verlag; 2001; p. 18-9.
6. Young DS. Effects of Drugs on Clinical Laboratory Tests. 5th
ed. Volume 1 and 2. Washington, DC: The American
Association for Clinical Chemistry Press 2000.
7. Schumann G, Bonora R, Ceriotti F, Férard G et al. IFCC
primary reference procedure for the measurement of catalytic
activity concentrations of enzymes at 37 °C. Part 5: Reference
procedure for the measurement of catalytic concentration of
aspartate aminotransferase. Clin Chem Lab Med
2002;40:725-33.
IN/0014 v01

ASAT (GOT) FS - Page 2