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Tissue Preservation

The document outlines methods for the collection and preservation of animal tissues post-necropsy, detailing various preservatives and techniques for different specimen types including mammals, birds, and herpetofauna. It emphasizes the importance of immediate examination or proper preservation using refrigeration, formalin, and alcohol solutions. Specific instructions for preserving organs, skins, and droppings are provided to ensure the integrity of biological specimens for analysis.

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Sajal Singh
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9 views8 pages

Tissue Preservation

The document outlines methods for the collection and preservation of animal tissues post-necropsy, detailing various preservatives and techniques for different specimen types including mammals, birds, and herpetofauna. It emphasizes the importance of immediate examination or proper preservation using refrigeration, formalin, and alcohol solutions. Specific instructions for preserving organs, skins, and droppings are provided to ensure the integrity of biological specimens for analysis.

Uploaded by

Sajal Singh
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Collection and Preservation of Animal Tissues

After performing necropsy of the animal, tissue samples are obtained


from the target organs for further examination and or preservation in
the laboratory

Examination may be conducted immediately after collection or

Tissues may be collected and preserved in desired preservatives for


subsequent examination and analysis

The following field methods and preservatives may be used for


preservation of biological specimens or their parts:-

o Refrigeration at approx 1 to 1.5°C is an excellent way to preserve


many biological materials for short duration
o Deep freezers
o Dry ice
o A variety of plastic containers /boxes are now available in different
sizes with thermocol lining for freezing and shiping biological
materials

COMMON PRESERVATIVES (Mammals)


For small specimens
Ethyl Alcohol 70%
Formalin 5%
For large specimens
Formalin 7 to 10%, also injection of preservative into internal
organs through circulatory route (perfusion)
For Skins
Thoroughly clean skins and expose them to dry air (Blow dry
air)
Sprinkle salt (NaCl) all over the skin underneath
Borax
Mixture of Borax-arsenic trioxide in equal proportions
For stomachs containing food material
5 to 10 % formalin depending on size of stomachs to be
preserved

For droppings and scats


To be dried immediately after collection
Fumigate with carbon disulfide

For reproductive organs


o AFA (Alcohol Formalin Acetic Acid solution), which is
(90 ml of 80% Alcohol; 10 ml of 37-40% Formalin and 5 ml of GAA)
o Bouin’s Fluid , which is
(250 ml of 37-40% Formalin and 50 ml of GAA)
o 10% Formalin
COMMON PRESERVATIVES (Birds)
For whole specimens
Ethyl Alcohol 70%
Formalin 5%
Both EA and formalin need to be injected internally if the specimens
are to be preserved for long duration

For skins
Borax
Mixture of arsenic and borax in nearly equal proportions

For stomachs
Formalin 5%
For droppings and pellets
Dry them to get rid of moisture
Fumigate with carbon disulfide if to be kept for long time
COMMON PRESERVATIVES (Herpetofauna)
For whole specimens
Ethyl Alcohol 70%
Isopropyl alcohol 35-40%
Formalin 5% (injecting internally of slitting the specimens)
Amphibian skins
Individual is killed using ether
Skin it and place skin in water
Place it on cardboard and dry

Salamander skins
Individual killed using chlorobutanol or 20% alcohol
Hardened with 5% formalin
Stored in 70% alcohol
Snake skins
For skins- Roll the skin flat on a cardboard and
store in 70% alcohol

Amphibian skins
Individual is killed using ether
Skin it and place skin in water
Place it on cardboard and dry

Salamander skins
Individual killed using chlorobutanol or 20% alcohol
Hardened with 5% formalin
Stored in 70% alcohol

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