C196-E058C
Prominence
Shimadzu
High Per for mance
Liquid Chromatograph
Pr omi nence
HPLC systems are currently used in a wide
variety of fields. Higher reliability in analysis
data and higher efficiency in total analysis
workflow are required for faster development of
new drugs, for better food safety, and for
meeting higher standards in environmental
regulations. Many analysis techniques use
LCMS, which requires a front-end HPLC
system to provide solvent delivery performance
in the micro to semi-micro range and
S up e ri o r C o mp o n e n t Pe rfo rma n ce
The SIL-20A/C autosamplers use a high-resolution
metering pump and total injection volume method for no
sample loss injection. The 6nL resolution ensures accurate
and reproducible analytical data.
Injection volume accuracy
Set Value Measured Value
Precision
Error
Injection Volume Area Repeatability
(uL)
(%RSD)
(uL)
(uL)
(%)
0.99
- 1.00
0.28
1.99
- 0.50
0.25
5.01
- 0.20
0.06
10
10.00
0.00
10
0.04
20
19.92
- 0.40
20
0.03
50
49.90
- 0.20
50
0.10
100
99.70
- 0.30
100
0.11
Autosampler
A specially treated outer needle surface and improvement
of needle seal composition greatly reduce sample
adsorption. This performance is critical for achieving the
high sensitivity of LC-MS/MS. In addition to that,
the optional multi-liquid rinsing demonstrates almost
undetectable sample carryover. The right chromatograms
show the carryover of highly adsorptive chlorhexidine.
SIL-20A provides good result even
with adsorptive compound.
With rinsing pump,
sample carryover is not detected.
Not detected
Sample carryover test using Chlor-hexidine
The SPD-20A detectors achieve the highest level of
detection sensitivity and linearity by electronic noise
elimination and a temperature-controlled flow cell.
This performance is ideal for everyday needs and
especially suitable for high-sensitivity analyses such as
impurity quantitation.
With the SPD-20AV, both D2 and W lamps can be lit
simultaneously so that high-sensitivity analysis for the full
UV to VIS wavelength range is possible.
Abs (AU)
2.5
1.5
Relative concentration (%)
0
0
50
100
150
Concentration linearity and impurity limit test for raw drug A
injectors with low sample carryover.
Prominence is a network-ready HPLC system
that meets the demands of today's advanced
users. Prominence features the world's first
Web control, fastest sample injection, and
highest detection sensitivity
performance...functions that surpass current
HPLC technology.
F l exi bl e s y s t em co n fig u ratio n s
Standard automated system
Prominence features a wide variety of components, enabling you to build
systems to meet any application need. Exceptional performance and functions
such as high throughput and low sample carryover enhance any system from
performing routine analysis to becoming a highly sophisticated LCMS front end.
The SIL-20 rack changer stores and transports up to 12 standard or deep-well
microtiter plates to the Prominence autosampler for a significant advantage in
sample capacity and throughput. Standard (ambient temperature) and cooler
models with temperature control from 4 to 40C are available.
Prominence will support multiple LC-20AB binary pumps, allowing an automated
pretreatment system with column switching to use gradient elution in both sample
pretreatment and analysis. With an additional pump, an automated system for
direct serum injection with online sample dilution capability can be configured.
Microplates in rack changer
High-capacity system with rack changer
Serum direct injection auto-pretreatment system
(Co-Sense for BA)
I n nova t i v e n etwo rk c o mp a tib ility
The world's first HPLC control by a web browser is
available with the CBM-20A. Use any computer with
Microsoft Internet Explorer 6.0 or later to control and
monitor the Prominence HPLC system. Connection to the
CBM-20A with a static IP address requires no special
software to control and monitor HPLC systems on the
network from the client PC.
Easily check the status of networked HPLC systems,
or monitor consumable parts for increased work efficiency.
HPLC control through Internet Explorer
Connection of HPLCs and PC workstations via the
Ethernet enhances flexibility of instrument installation to
efficiently use laboratory space, and provides a flexible
analytical network environment by controlling access to the
configured HPLC systems from client computers.
Networked Prominence systems support HPLC control,
run monitoring, maintenance information, data
management, and integrated analysis schedules.
Example of network environment using Prominence
Prom in e n c e Sp ec ifica tio n s
System Controller
CBM-20A (228-45012-xx)
CBM-20Alite (228-45011-38)
Number of connectable modules
5 (including a module in which CBM-20A lite is installed)
Event input/output
Input: 4, Output: 4
Input: 2, Output: 2
Solvent Delivery Unit
LC-20AD (228-45000-xx)
LC-20AT (228-45001-xx)
LC-20AB (228-45002-xx)
Pump type
Parallel double plunger pump
Serial double plunger pump
Parallel double plunger pump
Flow rate setting range
0.0001mL/min. - 10.0000mL/min.
0.001mL/min. - 10.000mL/min.
0.0001mL/min. - 10.0000mL/min.
Gradient System
LC-20AB (228-45002-xx)
LC-20AD/20AT
(with High-pressure gradient unit installed)
LC-20AD/20AT
(with Low-pressure gradient unit installed)
Gradient type
High pressure mixing
High pressure mixing
Low pressure mixing
Number of solvents mixed
2 or 3
On-line Degasser
DGU-20A 3 (228-45018-32)
DGU-20A 5 (228-45019-32)
Number of flow lines
Autosampler
SIL-20A (228-45006-xx)
SIL-20AC (228-45007-xx)
Injection system
Variable injection volume type (zero sample loss during injection)
Injection volume setting range
0.1 to 100uL (standard), 1 to 2000uL (optional)
Number of samples
processed
175 (1mL sample vials) + 10 (1.5mL vials)
105 (1.5mL sample vials) + 10 (1.5mL vials)
50 (4mL sample vials) 10 (1.5mL vials)
2 (96-well, 384-wells MTP/DWP) + 10 (1.5mL vials)
175 (1mL sample vials) + 10 (1.5mL vials)
70 (1.5mL sample vials) + 10 (1.5mL vials)
50 (4mL sample vials) 10 (1.5mL vials)
2 (96-well, 384-wells MTP/DWP) + 10 (1.5mL vials)
Sample cooling
none
4 to 40C (Block heating/cooling type, with dehumidification function built-in)
Column Oven
CTO-20A (228-45009-xx)
CTO-20AC (228-45010-xx)
Forced air circulation
Type
Absorption Detectors
SPD-20A (228-45003-xx)
Light source
D 2 lamp
SPD-M20A (228-45005-xx)
SPD-20AV (228-45004-xx)
190nm - 700nm
Wavelength range
Functions
(Room temperature - 10C) - (85C)
(Room temperature + 10C) - (85C)
Temperature control range
D 2 lamp, W lamp
190nm - 800nm
190nm - 900nm
Contour output
Spectral library
Max. plot
Dual wavelength detection in 190nm - 370nm, or in 371nm or higher
Ratio chromatogram output
Wavelength scan
Cell
Path length: 10mm, Volume: 12uL, Pressure: 12MPa
Path length: 10mm, Volume: 10uL, Pressure: 12MPa
JQA-0376
SHIMADZU CORPORATION. International Marketing Division
SHIMADZU DO BRASIL COMRCIO LTDA.
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SHIMADZU (HONG KONG) LIMITED
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Phone: 86-21-6472-8442 Fax. 86-21-6472-8648
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Overseas Offices
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Phone: 65-6778-6280 Fax. 65-6779-2935
Istanbul, Moscow
SHIMADZU SCIENTIFIC INSTRUMENTS (OCEANIA) PTY. LTD.
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Phone: 61(2)9684-4200 Fax. 61(2)9684-4055
URL http://www.shimadzu.com
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The contents of this brochure are subject to change without notice.
