Dentocult
Dentocult SM Strip mutans
Dentocult LB
Dentocult CA
Dentobuff Strip
Monitor infection with
bacteria causing caries
Dentocult SM Strip mutans
easy detection of mutans streptococci
allows early professional intervention
and prevention of caries
Test procedure
Dentocult SM Strip mutans
Mutans streptococci play an important role in the initiation and progression of dental caries
and they are considered the primary cause of bacteriological caries. This bacterium thrives
in the plaque that forms on the surface of teeth. It uses sugars to produce acids capable of
dissolving the tooth enamel, which will eventually become cavitated.
1. Place a bacitracin disk in the
selective culture broth.
2. Inoculate the strips.
Mutans streptococci are normally transmitted from adults to children. The earlier the colonisation
of teeth occurs, the higher the prevalence of dental caries. Therefore, it is important to screen
both parents and their children to establish a good preventive dental care.
Orion Diagnosticas Dentocult SM Strip mutans provides easy detection of mutans
streptococci from a saliva sample and plaque. The method is based on the use of a selective
culture broth and the adherence and growth of mutans streptococci on the test strip.
With the help of the test results, dental professionals can counsel the parents on oral hygiene
and ensure early professional intervention and prevention of caries.
Interpretation of results
Mutans streptococci adhere to the rough area of the strip in proportion to their density in
saliva/plaque. After incubation they are visible as light to dark blue, raised colonies on the
rough area of the test strip.
Saliva: Let the patient chew a parafn
pellet to stimulate the secretion
of saliva and to transfer mutans
streptococci from tooth surfaces into
the saliva. Press the round-tipped
test strip against the saliva on the
patients tongue.
Plague: Obtain a sample from an
interproximal site or a tooth surface
and spread it on the square-tipped
test strip.
Test strips for stimulated
saliva (round-tipped)
Product
Cat. No.
Dentocult SM Strip mutans
test strips for stimulated saliva (round-tipped), 10 pcs
test strips for plaque (square-tipped), 10 pcs
selective culture vials, 10 pcs
bacitracin discs, 50 pcs
parafn pellets, 10 pcs
67647
Literature
Bratthall D. Dental caries. markers of high and low risk groups and
individuals. Cambridge (UK): Cambridge University Press;1991,
Chaper 13.
Jensen B et al. A new method for the estimation of mutans
streptocci in human saliva. J Dent Res 1989;68:468-71.
Karjalainen S et al. Validation and inter-examiner agreement of
mutans streptocci levels in plaque and saliva of 10-year-old children
using simple chair-side tests. Acta Odontol Scand 2004;62:153-157.
Orion Diagnostica Oy
P.O.Box 83, FI-02101 Espoo, Finland
Tel. +358 10 42 995 , Fax +358 10 429 2794
www.oriondiagnostica.fi
3. Place the strips, attached back
to back to the cap, in the selective
culture broth and recap the vial.
Pienihkkinen K, Jokela J. A simple method for monitoring mutans
streptococci in young children. Eur J Oral Sci 1995;103:61-2
Sderling E et al. A chairside strip test in monitoring transmission of
mutans streptocci. J Dent Rres 2002;81 (Spec Issue A):ABS No 745.
Thorhild I et al. Prevalence of Streptococcus mutans in mothers
and in their preschool children. Int J Paediatr Dent 2002;12:2-7.
4. Incubate at 35-37C for 48 h
with caps slightly open. Interpret
the result.
748-03E, Erweko 4/2006
Strips for plaque
(Square-tipped)
