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Some Important Formula-Base Hospital-Karawanella: 01.0.3 Molar Sodium Citrate Solution

The document provides two formulas for alcohol-based hand rub solutions based on WHO guidelines. Formula 1 contains 96% ethanol, 3% hydrogen peroxide, and glycerol in distilled water. Formula 2 replaces ethanol with isopropyl alcohol. The formulas are prepared by measuring the specified amounts of alcohol, hydrogen peroxide, and glycerol into a bottle, then filling with distilled water and sealing until the solution is divided into smaller containers after 72 hours.

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Saman Vithanage
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65 views1 page

Some Important Formula-Base Hospital-Karawanella: 01.0.3 Molar Sodium Citrate Solution

The document provides two formulas for alcohol-based hand rub solutions based on WHO guidelines. Formula 1 contains 96% ethanol, 3% hydrogen peroxide, and glycerol in distilled water. Formula 2 replaces ethanol with isopropyl alcohol. The formulas are prepared by measuring the specified amounts of alcohol, hydrogen peroxide, and glycerol into a bottle, then filling with distilled water and sealing until the solution is divided into smaller containers after 72 hours.

Uploaded by

Saman Vithanage
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as ODT, PDF, TXT or read online on Scribd
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Some Important Formula-Base Hospital- Karawanella

01.0.3 molar sodium citrate solution


sodium citrate

88.2 g

freshly boiled cooled water to

1000ml

02.alcoholic hand rub(based on W.H.O guidelines on hand hygiene in health care)


formula 1
ethanol 96 V/V

833.3 ml

hydrogen peroxide 3

41.7 ml

glycerol 98

14.5 ml

distilled water or
freshly boiled & cooled water to

1000ml

(note; shake the flask gently to mix the content)


formula 11
isopropyl alcohol(or surgical spirit)

751.5ml

hydrogen peroxide 3

41.7 ml

glycerol 98

14.5ml

distilled water or
freshly boiled & cooled water to

1000ml

(note; shake the flask gently to mix the content)


Preparation
- The alcohol for the chosen formulation is poured into the large bottle or tank up to the graduated
mark.
- H2O2 is added using the measuring cylinder.
- Glycerol is added using a measuring cylinder. As the glycerol is very viscous and sticks to the
walls of the measuring cylinder, it can be rinsed with some sterile distilled or cold boiled water to
be added and then emptied into the bottle/tank.
The bottle/tank is then topped up to the corresponding mark of the volume (10-litre or 50-litre) to
be prepared with the remainder of the distilled or cold, boiled water.
The lid or the screw cap is placed on the bottle/tank immediately after mixing to prevent
evaporation.
After mixing, the solution is immediately divided into smaller containers (e.g. 1000, 500 or 100
ml plastic bottles). The bottles should be kept in quarantine for 72 hours. This allows time for any
spores present in the alcohol or the new or re-used bottles to be eliminated by H2O2.

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