C101-E070I

UV-VIS Series
Accessories

Shim adzu
UV-VIS Spectrophotometers

Con tents

Contents

Liquid Samples
P 03 - 1. Basic Measurement

P 05 - 2. Short Optical Path Measurement

P 06 - 3. Long Optical Path Measurement

P 07 - 4. Micro-volume Measurement

P 09 - 5. Accessories for Constant-Temperature Measurement

P 12 - 6. Automatic Analysis

P 15 - 7. Onsite Measurement (Optical Fiber Application Measurement)

Suspension and Opaque Samples (solid)

P 16 - 8. Suspension and Opaque Sample Measurement

P 22 - 9. Special Accessories for SolidSpec-3700/3700DUV

P 23 - 10. Printer

P 24 - 11. Interfaces, Cable

Software
P 25 - 12. Software

1. Basi c M eas u r e m e n t

Liquid Samples
1 . B a s i c M e a su reme n t
Accessories that are used with all Shimadzu spectrophotometers.
Cells
A square cell with a stopper is generally used to measure
volatile liquid samples, and a microcell is used to measure a
sample with small volume.
Sample cells are made of one of the three materials listed
below. The wavelength range for each type of material has
been established as follows:

Samples are placed in cells for measurement. The figure below shows the ten
sample cell shapes. Generally, the rectangular cell with an optical path length
of 10 mm is used. In cases when absorption is low, a cell with a longer optical
path length is used, whereas when absorption is high, a cell with a shorter
optical path length is used. The relationship between absorption strength
(absorbance) and cell length is described below. By utilizing this formula,
an appropriate optical path length can be determined.

Fused silica (S cells) : 190 - 2500 nm

Glass (G cells) : 320 - 2500 nm
Fused silica (IR cells) : 220 - 3200 nm

Absorbance (A) = E C L
E : Absorption coefficient (a constant for given samples)
C : Concentration of sample
L : Cell length
Optical
path (L)
10mm

Required Sample
Volume
2.51 to 4.0mL

20mm

5.0 to 8.0mL

50mm

12.5 to 20.0mL

100mm

25.0 to 40.0mL

Square cell with stopper

10mm

2.5 to 4.0mL

Semi-micro cell

10mm

Semi-micro black cell

Description

Square cell

Super micro black cell

Micro black cell

Cylindrical cell

Short path cell

Type

Fused silica (S)

Glass (G)

Fused silica (IR)

200-34442

200-34565

200-66579-01

200-34446

200-34446-01

200-66579-02

200-34944

200-34944-01

200-66579-03

200-34676

200-34676-01

200-66579-04

200-34444

200-34444-01

200-66579-21

1.0 to 1.6mL

200-66501

200-66501-01

200-66579-11

10mm

1.0 to 1.6mL

3'

200-66551

5mm

25 to 100L*1

7'

208-92116

10mm

50 to 200L*2

200-66578-11

10mm

50 to 400L

200-66578-12

10mm

3.8mL

200-34448

(silica window)

200-34448-01 (glass window)

200-66579-31 (IR silica window)

20mm

7.6mL

200-34472

(silica window)

200-34472-01 (glass window)

200-66579-32 (IR silica window)

50mm

19.0mL

200-34473-01 (silica window)

200-34473-03 (glass window)

200-66579-33 (IR silica window)

100mm

38.0mL

200-34473-02 (silica window)

200-34473-04 (glass window)

200-66579-34 (IR silica window)

200-34660-01

200-34662-01

200-66579-05

200-34655

200-34662-11

200-66579-06

200-34449

200-34449-01

200-66579-07

1mm

0.3 to 0.4mL

2mm

0.5 to 0.8mL

5mm

1.3 to 2.0mL

200-66579-12

12.5
25

45

12

25

12
12

L
2

.5

2
2

.5

12

12.5

4'

7'

Micro cell for nucleic acid quantitation (5L,10L), Black micro cell (70L)

Unit: mm
*1 50 ~ 100L with UVmini
*2 100 ~ 200L with UVmini

The optimal micro cell for quantitation of nucleic acids.

Micro cell for nucleic acid quantitation (5L, optical path 5mm) (P/N 046-25302-12)
Micro cell for nucleic acid quantitation (10L, optical path 10mm) (P/N 046-25302-02)
Micro cell for protein quantitation.
Black micro cell (70L, optical path 10mm) (P/N 046-25302-11)
*Use with the standard cell holder provided. It cannot be used together with the multicell holder, the
sample compartment unit, the thermoelectrically temperature-controlled cell holder TCC-240A, or
the 6-cell type thermoelectrically temperature-controlled cell positioner CPS-240A.
*We recommend that these micro cells be used within the absorbance range of 0.2 to 1.0.
*As the quantity of light passing will be reduced when using the micro cell, the optical
specifications of the system may not be met.
*Insert a cell containing the buffer before measuring samples, and carry out baseline correction.
5L cell

10L cell

70L cell

1. Basi c Mea s u r e m e n t

Liquid Samples
1 . B a s i c M e a s u reme n t
Film Holder

(P/N 204- 58909)

Holds thin samples, such as films and filters.

Note: 1. The Alternate Sample Compartment (P/N 206-60184-07) is
necessary for the UVmini-1240/1240V, MultiSpec-1500.
2. Direct Detection Unit (See page 22) is necessary for the
SolidSpec-3700/3700DUV.

Didymium Filter ( P/ N 202- 30242- 09)

Holmium Filter ( P/ N 202- 30242- 05)
These filters are used to check the instrument.

Spectrum using a didymium filter

Four-Cell Sample Compar tment Unit with Holder

Spectrum using a holmium filter

(P/N 206-23670-91)

Holds four 10 mm path cells, which are manually selected by sliding the mount.
Note: Not applicable to SolidSpec-3700/3700DUV.

Alternate Sample Compar tment

( P/ N 206 - 6 0 1 8 4 - 0 7 )

(U V mini Se r ies , Mult i Spec- 1500 onl y)

Holds such sampling accessories as the super microcell holder, micro flow-thru cell, long-path
rectangular cell holder, cylindrical cell holder, film holder, and the constant-temperature cell holder.

2. Shor t O ptic a l P a t h M e a s u re me n t

Multicell Sample Compar tment (6 Cell)

Holds up to six 10 mm square cells. No temperature control capability.
Number of cells : 6 on the sample side and 1 on the reference side. Reference side is not
used on the UVmini-1240.
Cells are not included.
P/N

UVmini series

3600 series

MultiSpec-1500

206-60605-02

Yes

1600/1700/1800 series 2400/2500 series

No

No

No

Yes

206-69160-01

No

Yes

Yes

Yes

No

P/N 206-69160-01

2 . S h o rt O p t i ca l P a th Mea s u rement
Used to measure samples with absorbance too high to be measured with a standard 10 mm square cell.

Absorption spectra of toluene (C6H5CH3) measured using a cell with either a 1 or 10 mm optical path length

Spacer for Shor t-Path Cell

Three types of spacers are available to provide path lengths of 1, 2, and 5 mm.
The spacer is to be sandwiched between the cell and the inner wall of the square cell
holder. (See the figure.)
P/N (Spacer)

Path length of cell

204-21473-03

1 mm

204-21473-01

2 mm

204-21473-02

5 mm

Optical Path length of cell

3. Lo ng O pti c a l P a t h M e a s u r eme n t

Liquid Samples
3 . Lo n g O p t i c a l P a th Mea s u rement
When the concentration of a sample is too low to be measured using a
standard 10 mm optical path length cell, a cell with a longer optical
path length is used for measurement at the optimal absorption
sensitivity. As shown in the figure on the right, the absorption spectrum
of potassium permanganate becomes clearer when a 100 mm cell is
used instead of a 10 mm cell.

Optical path length: 100 mm

Optical path length: 10 mm

Spectrum of potassium permanganate

Universal Rectangular Cell Holder, Four-Cell Type

(P / N 2 04 -27 20 8)
Holds four rectangular cells: 10, 20, 30, 50, 70 and 100 mm in path length.
Note: 1. The Four-Cell Sample Compartment (P/N 206-23670-91) is necessary.
2. If the measurement requires a long rectangular cell at the reference side, a Reference
Long Rectangular Cell Holder (P/N 204-28720) is necessary. 70mm and 100mm cells
can not be used for the UVmini Series.
3. Not applicable to SolidSpec-3700/3700DUV.

Reference Side Rectangular Long Pass Absorption Cell Holder

(P / N 2 04 -28 72 0)
If using a four-cell rectangular long pass absorption cell holder, only use a reference side cell holder if necessary.
Note: Not applicable to SolidSpec-3700/3700DUV.

Long-Path Rectangular Cell Holder

(P / N 2 04 -23 11 8-0 1)
Holds two rectangular cells: 10, 20, 30, 50, 70 and 100 mm in path length.
Note: The Alternate Sample Compartment (P/N 206-60184-07) is necessary for the
UVmini Series, and MultiSpec-1500. 70mm and 100mm cells can not be used for
the UVmini Series.

Cylindrical Cell Holder

(P / N 2 04 -06 21 6-0 2)
Holds two cylindrical cells: 10, 20, 50 and 100 mm in path length.
Note: The Alternate Sample Compartment (P/N 206-60184-07) is necessary for the
UVmini Series, and MultiSpec-1500.

4. Mi cr o-vol u m e M e a s u r e m e n t

4 . M i c ro - v o l u me Mea s u reme n t
The following accessories are used to measure micro-volume samples. This technique allows a sample size as small
as 50L to be measured using a super micro cell. Thus, this technique can be used for biochemical analysis in which
minute samples are measured.
Super Micro Cell Holder
Holds micro or super micro cells for measurement of extremely small volume samples such
as DNA and proteins. The minimum volume required for both cell types is 50L when the path
length is 2 mm. The maximum sample volume is 400L for the micro cell and 120L for the
super micro cell.
Applicable cell : micro or super micro cell. Cells are not included.
Mask : Choice of 1.5W x 1H mm and 1.5W x 3H mm
Mask
Optical
path hole
Bottom
of cell

Cell
P/N 206-14334

Adjustment screw

Holder

P/N

Hexagonal wrench for

adjusting height

Supermicro Cell Holder

UVmini
series

1600/1800 2400/2500
series
series

3600

MultiSpec
-1500

Solid
Spec

206-14334

No

Yes

Yes

Yes

Yes

Yes

206-14334-01

Yes

No

No

No

No

No

Note: The Alternate Sample Compartment (P/N 206-60184-07) is necessary for the UVmini Series, and MultiSpec-1500.

( P/ N 206- 55050- 91)

(U V -1700 only )
Holds supermicro cells for measurement of extremely small volume samples.
Samples of 25 to 200 L can be measured, depending on the type of black cell used.
Applicable cells: 7,7', and 8 in the cell list (page 3)

Micro Cell Holder with Mask

( P/ N 204- 06896 )

For semi-micro cells and micro cells with a maximum path length of 3mm.
Mask width can be adjusted.
Note: The Alternate Sample Compartment (P/N 206-60184-07) is necessary for the
UVmini Series, and MultiSpec-1500. Only a cell of 4mm in path width is used for
the UVmini Series.

4. Mi cr o-vol u m e M e a s u r e m e n t

Liquid Samples
4 . M i c ro - v o l ume Mea s u reme n t
3L Capillar y Cell Kit for Ultramicro Volume Measurement
(P /N 20 6-6 97 46 )
(UV -16 00 /17 00 /18 00 ser i es, 2400/ 2500 ser i es, 36 0 0 , S o l i d S p e c o n l y )
Recommended for small volume and precious samples, such as in biological applications.
The minimum sample volume required is 3L when the tube closure is used (theoretical value).
Solution sample is aspirated into the capillary cell and the cell is directly subjected to
measurement.
The holder is the same size as a 10 mm square cell and can be mounted to the standard
cell holder.
Supplied with 100 capillaries (made of quartz) and a tube closure.
Note: Effective optical path length is typically about 1/20 of 10mm square cell.

8/16 Series Micro Multi-Cell Holders and Cells

(UV -16 00 /17 00 /18 00 ser i es, 2400/ 2500 ser i es, 36 0 0 o n l y )
C ell Holder s
P/N

Model
8/16 Series Micro Multi-cell Holder MMC-1600

206-23680-91

8/16 Series Constant Temperature Micro Multi-cell Holder MMC-1600C

206-23690-91

This cell holder holds one micro multi-cell, either 8 or 16 cell, for micro-volume
measurement.Two types of micro multi-cell holders are available, the standard type (MMC1600) and the constant temperature water circulation type (MMC-1600C).
Applicable temperature range : 10-60C (C type)
Temperature deviation between circular water and cell : max. 3C (C type)
Temperature stabilizing time : 15min or less (C type)

Micro Multi-cells
Standard sample volume

P/N

8 Series Micro Multi-cell; optical path length 10mm, cell volume 100L

208-92089

16 Series Micro Multi-cell; optical path length 10mm, cell volume 100L

208-92088

8 Series Micro Multi-cell; optical path length 5mm, cell volume 50L

208-92086

16 Series Micro Multi-cell; optical path length 5mm, cell volume 50L

208-92085

There are two types of micro multi-cells available in both the 8 Series and the 16 Series
models, a 50L type and a 100L type. The cell intervals of the 8 Series Micro Multi-cell
are applicable for use with 8 x 12 well microplates and 8-channel pipettes. Microplate
samples aspirated into multi-channel pipettes can be injected directly into the cells for
measurement.
Micro-volume samples can be measured
Support for commercial microplates and micro pipettes
(with 8 Series micro cells)
Up to 16 samples can be measured at a time (with 16 Series micro cell)

Micro Cell Mask for Standard Six-Cell Holder

(P /N 20 6-6 68 28 )
(UV mini Se r ies , Mult i Spec- 1500 onl y)
Stops down the measuring light beam for measurement with 4mm-wide path micro cells
installed in the standard six-cell holder.
Applicable Cell:10mm Semi-micro cell (quartz) (P/N 200-66501)
10mm Semi-micro cell (glass) (P/N 200-66501-01)
10mm Semi-micro black cell (quartz) (P/N 200-66551)

5. Accessor i e s f o r C o n s t a n t - T e mp e ra t u re M e a s u re me n t

5 . A c c e s s o ri es fo r C o n stan t-T e mperat ure M easurement

The following accessories are used for biological component
measurement, enzymatic activity measurement and reaction
rate analysis for which the sample temperature must be kept
constant. The figure shows an example in which enzymatic
activities are measured by combining the UV-1700 and the
6-cell thermoelectrical temperature-controlled cell positioner
CPS-240A.

Using a cell positioner, reactions in the six cells could be measured in parallel.
Sample: serum cholinesterase activity of solution was measured.
Measurement time: 200 seconds.

Constant-Temperature Cell Holder

( P/ N 20 2 - 3 0 8 5 8 - 0 4 )

Maintain a sample cell and reference cell at a desired, uniform temperature by circulating
constant-temperature water.
Temperature range : 5 to 90C (depends on the performance of the constant-temperature
water circulator)
Accepts a pair of 10 mm square cells
Connection joint outer diameter : 6mm and 9mm
Note: 1. The Alternate Sample Compartment (P/N 206-60184-07) is necessary for the UVmini
Series, and MultiSpec-1500.
2. Not applicable to SolidSpec-3700/3700DUV.

Constant-Temperature Four-Cell Holder

(P/N 204-27206-02)

Maintain four sample cells and a reference cell at a desired, uniform temperature
by circulating constant-temperature water.
Temperature range : 5 to 90C (depends on the performance of the constant-temperature
water circulator)
Accepts four 10 mm square cells plus a reference cell
Connection joint outer diameter : 9mm
Note: 1. The Four-Cell Sample Compartment (P/N 206-23670-91) is necessary.
2. Not applicable to SolidSpec-3700/3700DUV.

5. Accessor i e s f o r C o n s t a n t -T e mp e ra t u re M e a s u re me n t

Liquid Samples
5 . C o n s t a n t - T e mp era tu re Mea s urement
CPS-240A Cell Positioner, Thermoelectrically Temperature Controlled

( P / N 2 0 6 - 2 3 7 6 0 - )

1) Supports time-course monitoring of up to 6 samples

The multicell holder and the single cell holder do not have
temperature control capability. This option permits measurement of
up to six samples under constant-temperature conditions.
2) Supports enzyme activity assay
Combination with the appropriate Kinetics Program Pack or
software permits measurement of enzyme kinetics for up to six
samples under constant-temperature conditions.
Number of cells : 6 on the sample side (temperature controlled)
1 on the reference side (temperature not controlled)
Temperature range : 16 to 60C
Temperature display accuracy (difference from the true value): 0.5C
Temperature control precision (variation of temperature): 0.1C
Ambient temperature : 15 to 35C
Note: 1. Square cells are not included in the standard contents.
2. A USB adaptor CPS (P/N 206-25234-91) is required for UV-1800.
3. Not applicable to SolidSpec-3700/3700DUV.

TCC-240A Thermoelectrically Temperature-Controlled Cell Holder

Uses Peltier effect for controlling the sample and reference
temperature, so no thermostated bath or cooling water is required.
Number of cells : One each on the sample and reference sides
(temperature controlled)
Temperature range : 7 to 60C
Temperature display accuracy (difference from the true value): 0.5C
Temperature control precision (variation of temperature): 0.1C
Note: 1. Square cells are not included in the standard contents.
2. Not applicable to SolidSpec-3700/3700DUV.

NTT-2200P Constant-Temperature Water Circulator

(P/N 208-97263)

Circulates temperature-controlled water to a constant-temperature cell holder.

Temperature range: Ambient +5C to +80C
Temperature control precision: 0.05C~
Max. pumping rate: 27/31 L/min, 9.5/13 m (50/60 Hz)
External circulation nozzle: 10.5 mm OD (both outlet and return)
Tank capacity: About 10 L (9 L during use)
Safety features: Detection of over-temperature of Upper or Lower limits, Detection of heater
wire malfunction, Protection of heating too little circulating water, Detection
of sensor malfunction, Independent over treat protection, Over current
circuit protector
Standard accessories: Lid with handles, Rubber hose (4m), Hose clamps (4pc.),
Instruction manual
Dimensions: 270W x 560H x 400D (mm)
Power requirements: 100 VAC, 1250 VA, with 1.7 m power cord and grounded plug

10

( P / N 2 0 6 - 2 3 7 8 0 - )

S-1700 Thermoelectric Single Cell Holder

(P / N 200V:2 06 -23 90 0-3 8, 100V: 206- 23900- 91)
(U V -1600/1 70 0/1 80 0 s er i es, 2400/ 2500 ser i es, 3600 , M u l t i S p e c - 1 5 0 0 o n l y )
This cell holder permits setting of a temperature program to increase and decrease the
sample cell temperature.
The thermoelectric system allows prompt control of sample temperature between 0C and
110C.
Temperature increase/decrease speed can be changed using 12 settings, which means the
holder can be used in analysis of melting curves for nucleic acids, etc. that occur during
quick as well as slow heating (or cooling).
A stirrer is also provided to ensure uniform temperature distribution throughout the cell.
Cooling water circulation is required for Peltier element cooling. And though tap water can
be used, it is recommended that a commercially available constant-temperature water
circulator be used, as the following conditions must be fulfilled to exact maximum
performance from the S-1700.
Cooling water specification: 20 2C
Water flow: 4.8L / min or more

Temperature is not controlled at the reference side.

Cells are not supplied. Please use 10mm square tight-sealing cells (a Hellma product).
Minimum Sample Volume Required

Type

Optical Path

110-QS-10

10mm

3.5mL

115B-QS-10

10mm

400L

Temperature accuracy in cell (when room temperature is 25C)

Within 0.25C (0 to 25C)
Within 1%C of set value (25 to 75C)
Within 2%C of set value (75 to 110C)

Tm Analysis Software ( P/ N 206- 57476- 91)

(U V -1600/17 00 /18 00 se r i es, 2400/ 2500 ser i es, 3600 + S - 1 7 0 0 o n l y )
This software works with the S-1700 and accumulates temperature-versus-absorbance curve data at the PC to analyze the Tm (melting
temperature) of nucleic acids such as DNA and RNA. The figure below is a typical setup for this software.
RS-232C cable (P/N 200-86408) is needed to connect the PC to the S-1700.
Note: 1. USB cable (P/N 088-52848-32) is required for UV-1800 to connect the PC to the UV-1800.
2. RS-232C cable (P/N 200-86408) is required for UV-1700 to connect the PC to the UV-1700.

PC

Spectrophotometer

RS-232C

S-1700

11

6. Autom ati c A n a l y s i s

Liquid Samples
6 . A u t o m a t i c A n aly s is
The following accessories are used to continuously send samples to a sample compartment for measurement.
The sipper unit automatically supplies sample solutions in cells using a step motor-driven peristaltic pump. When this
unit and the auto-sample changer are used simultaneously, up to 100 samples can be measured automatically in
approximately 20 minutes. The micro flow cell can also be used for continuous measurement of the column effluent.
Sipper Units
4 types of sipper units with different cell types are available.The stepping motor-driven peristaltic pump ensures reliable and smooth aspiration of sample solution.
S i p p er L
Single pass, 10 mm light path, and 2 mL standard sample volume required (minimum 1 mL).
S i p p er T
Triple pass, 10 mm light path, and 1.5 mL standard sample volume required (minimum 0.8 mL).
S i p p er C
Provided with a constant-temperature jacket, 10 mm light path, and 2.5 mL standard sample
volume required (minimum 1 mL).
S i p p er U
Supermicro type, single pass, 10 mm light path, and 0.5 mL standard sample volume required
(minimum 0.3 mL).
Suction, drain and stabilizing times are digitally set so that these parameters can be
optimized for different sample viscosity.
Note: The use of a Solenoid Valve (P/N 204-06599-01) and the SWA-2 Sample Waste Unit (P/N
206-23820-91) are recommended when strong acids, strong alkalis,
or organic solvents are to be measured.

Type and configuration of flow cells

160L (P/N 206-23790-91)

160T (P/N 206-23790-92)

Sample outlet

160C (P/N 206-23790-93)

Constanttemperature
water outlet

Sample outlet

Sample outlet
Luminous
flux diaphragm

Luminous
flux diaphragm

Luminous
flux diaphragm

Luminous
flux diaphragm

Reflecting
mirror
Optical
path

Sample outlet
Sample
outlet

Luminous
flux diaphragm

Luminous
flux diaphragm

Optical
path

Optical
path

Inlet
Inlet

160U (P/N 206-23790-94)

Constanttemperature
Sample water inlet
inlet

Optical
path

Inlet

(Required Sample Volume : 2.0mL) (Required Sample Volume : 1.5mL) (Required Sample Volume : 25mL) (Required Sample Volume : 0.5mL)
Cross-section of a L-type flow cell Cross-section of a T-type flow cell Cross-section of a C-type flow cell Cross-section of a U-type flow cell
Standard type
Triple-pass type
Constant-temperature type
Supermicro type
L type : Standard flow cell
T type : Triple-pass flow cell
The light beam passes through the flow route 3 times and the total optical path is 10mm. Air bubble and sample removal operations are smooth
since the cell shape is vertically elongated.
C type : Constant-temperature flow cell
The flow cell has a double structure and isothermal water is circulated around the internal structure to maintain temperature.
U type : Supermicro flow cell
This model is used when the volume of sample is very small. Noise is somewhat high compared to the L model since the light beam furher cut.

12

Syring e Sipper N (Normal-temperature-type)

(P / N 206-2 38 90 -91 )

Syringe Sipper CN
(Constant-temperature, water-circulating type)
(P / N 206-2 38 90 -92 )
(U V mi n i s er ie s, UV-1 60 0/ 1700/ 1800 ser i es,
2400/250 0 s er ie s, 3 60 0 onl y)
Two types of syringe sippers are available, the normal-temperature
type (N) and the constant-temperature, water-circulating type (CN).
The sipper unit employs a syringe pump system. The liquid contact
surfaces are composed of Teflon, glass, or quartz, imparting excellent
chemical resistance and ease of maintenance, and allowing
measurement of almost any sample type. Further, the extremely high
repeatability of sipping volume (repeat precision: 0.03mL) makes it
ideal when performance validation is required.
Flow cell available separately. Choose from the recommended flow
cells listed below.
Circulation water temperature range : ambient to 60C (CN type)
Note: The Alternate Sample Compartment (P/N 206-60184-07) is
necessary for the UVmini Series.
Square cell (Ultra-micro) can not be used for the UVmini Series.

ASC-5 Auto Sample Changer

Cell Type

P/N

Optical Path Dimensions of Standard Required

Aperture
Sample Volume
Length
10mm

2mm

0.9mL

208-92113

10mm

3mm

1.0mL

Square (Semi-micro) 208-92005

10mm

H11 x W3.5mm

5.0mL

Square (Ultra-micro) 208-92114

Square (Micro)

The flow cell can be changed independently for

excellent ease of maintenance.
Excellent chemical resistance allows
measurement of almost any sample.
Excellent repeat sipping of fixed volumes.

( P/ N 206- 23810- * *)

Combine with a Sipper 160 to build an automated multisample

spectrophotometry system.
The aspirating nozzle is programmed to move in the X, Y, and
Z (vertical) directions.
Up to 8 sets of operational parameters, including the size of racks
and the number of test tubes, may be memorized in the battery
back-up protected files.
Up to 100 test tubes may be set together on the rack.
Note: 1. A commercially available test tube stand, with a footprint
smaller than 220 x 220mm, is applicable.
2. Not applicable to MultiSpec-1500 and SolidSpec-3700/3700DUV.
3. An ASC USB adaptor (P/N 206-25235-91) is required for UV-1800.

SWA-2 Sample Waste Unit

(P /N 206-23 82 0-9 1)
The SWA-2 is a universal aspirator having a built-in vacuum pump.
Recommended for use with sippers and Solenoid valve
(Fluoropolymer) for acids,
bases, and organic solvents.
Note: 1. Where tap water pressure fluctuates, it is better to use the
SWA-2 in place of an ordinary aspirator.
2. Not applicable to SolidSpec-3700/3700DUV.

13

6. Autom ati c A n a l y s i s

Liquid Samples
6 . A u t o m a t i c A n aly s is
Solenoid Valve (Fluoropolymer)
(P / N 2 04 -06 59 9-0 1)
This accessory is required because strong acid, strong alkali and ester solutions cannot be
measured due to a lack of chemical resistance in the sipper unit and the pump for the
thermoelectrical temperature-controlled sipper unit. This accessory requires the use of the
sample waste unit SWA-2 (P/N 206-23820-91).
Note: Not applicable to SolidSpec-3700/3700DUV.

10 mm Micro Flow-Thru Cell with Holder

(P / N 2 04 -06 22 2)
10 mm path length and 0.3 mL inner volume.

5 mm Micro Flow-Thru Cell with Holder

(P / N 2 04 -06 22 2-0 1)
5 mm path length and 0.15 mR inner volume.
Note: 1. The Alternate Sample Compartment (P/N 206-60184-07) is necessary for the
UVmini Series, and MultiSpec-1500.
2. Not applicable to SolidSpec-3700/3700DUV.

Front Panel with Holes

( P/ N 204- 27588- 03)

(UVmini series, UV-1600/1700/1800 series, 2400/2500 series, MultiSpec-1500 only)
Allows the tubes of a flow-thru cell, for example, to be connected through the front panel of the
instrument.
Note: 1. The Alternate Sample Compartment (P/N 206-60184-07) is necessary for the UVmini
Series, and MultiSpec-1500.
2. Not applicable to SolidSpec-3700/3700DUV.

Flow Cell for Liquid Chromatography ( P / N 2 0 6 - 1 2 8 5 2 )

(U V -1 60 0/1 70 0/1 80 0 ser i es, 2400/ 2500 ser i es, 36 0 0 o n l y )
This flow cell allows UV-VIS detection for high performance liquid chromatography at variable
wavelengths.
Inner diameter: 1 mmf, optical path length: 10 mm, volume: 8L
Sample side: flow cell, reference side: cell holder with mask
Note: 1. Not applicable to UVmini Series, MultiSpec-1500, SolidSpec-3700/3700DUV.
2. UV-1600/1700 series: when connecting an integrator, an analog signal output interface
(P/N 204-04757) is required.
3. UV-1800: when connecting an integrator, an analog signal output interface
(P/N 206-25233-91) is required.
4. UV-1601PC/1650PC/2401PC/2501PC/2450/2550 cannot send analog signals.

14

7. O nsi te Mea s u r e m e n t ( O p t i ca l F ib e r Ap p lic a t io n M e a s u re ment)

7 . O n s i t e M e as u reme n t (O p tica l Fiber Applicat ion M easurement )

Single 600m dia. fiber achieves high throughput.
The general-purpose SM905 fiber connector allows operation with other SM905-compatible probes.

Crossflow Cell for Process Monitor System

Flow of sample

(P /N 206-5 35 70 -13 )
(UV -2400 /25 00 se r ies , 3600 onl y)
Optical path length is continuously variable from 1 mm to 15mm to allow
monitoring of samples of any concentration.
Measuring wavelength range: 230~800nm (For 2m fiber length)
Optical path length: 1~15mm (variable)
Sample temperature: < 130C
Pressure resistance: <1.72 MPa (17.6kg/cm2)
Material: 316SS

SMA905
connector

Pipe connectors: < 1/2 inch

Optical material: <synthetic quartz

Optical Fiber Cou pler

UV -2401 /24 50 /25 01 /25 50, 3600 onl y
(P /N 206-5 41 75 -91 )
Optical fiber application system is connected to a UV-VIS spectrometer using a high-precision optical fiber coupler. This optical fiber coupler has
been designed for the UV-2400/2450/2500/2550, and 3600 to guarantee high throughput and stability.

Configurations

K :Required

Description

P/N

UV-2450

206-24300-**

UV-2550

206-24400-**

UV-3600

206-23000-**

Optical fiber coupler*1

(0.5mm optical fibers, 2 pcs. )

Process monitor
system

choose among these

206-54175-91

K
K

Crossflow cell

206-53570-13

2m optical fibers (2 pcs. a set)*2

206-53875-92

5m optical fibers (2 pcs. a set)*2

206-53875-93

10m optical fibers (2 pcs. a set)*2

206-53875-94

20m optical fibers (2 pcs. a set)*2

206-53875-95

choose among these

Note: If fibers are connected, the MultiSpec basic specifications for measuring accuracy, stray light, etc. are out of guarantee.
*1Crossflow cell is designed to have maximum throughput at room temperature.
*2Light intensity available for transmission decreases according to the fiber length by approximately the following ratios.
Fiber length

Decrease in UV range (100% at 0.5 m)

Decrease in visible range (100% at 0.5 m)

2m

80%

95%

5m

60%

92%

10m

36%

90%

20m

13%

80%

15

8. Suspensi o n a n d O p a q u e S a mp le M e a s u re me n t

Suspension and Opaque Samples

8 . S u s p e n s i o n an d Op aq u e S a mple M easurement
Measurement of suspension samples is difficult due to the scattering of reflected light by fine particles in the solution.
Integrating sphere are used in this type of analysis. The glass method involves the placement of a scattering board
behind the sample, resulting in the equalization of scattering coeffcients of the reference side and sample side. As
shown in Figure 1, the integrating sphere method involves a barium sulfate-coated sphere that draws the scattered
light, allowing all of the light to reach the detector. Since light cannot penetrate opaque samples, it is reflected on the
surface of the samples. Figure 2 shows one case in which incoming light is reflected symmetrically with respect to the
normal line (forward reaction), and another case in which the incoming light is scattered in different directions. The
former is referred to as specular reflection and the latter is referred to as diffuse reflection.

Control beam
M2

Suspension
sample

M2

Ip+Id

Water

I0
Side-on type
photomultiplier

Standard
white board
Sample
beam

Integrating
sphere

Standard
white board

M1
Figure 1. Semi-transparent sample measurement using the integrating sphere method

Reflected light

Incoming light

Incoming light
Reflected light

(a) Specular reflection

(b) Diffuse reflection

Figure 2. Specular reflection and diffuse reflection

16

(solid)
8 . Su s p e n s i o n an d Op aq u e S a mple M easurement
Integrating Sphere
With this instrument, a turbid sample is placed in front of the
incoming light window as shown in Figure 1, and the reflectance of
an opaque sample is measured by placing it as shown in Figure 3.
In this case, when light is directed at the sample at 0 degrees,
diffuse reflectance is measured, and when light is directed at 8
degrees, total reflectance (specular and diffuse reflectance) can be
measured.

Control beam
M2
M2

Side-on type
photomultiplier

I0

Standard
white board
Sample
beam

Idr

Sample
Integrating
sphere

M1
Figure 3. Opaque sample measurement using the integrating sphere
method

Integrating sphere not used

Integrating sphere used

Spectrum of a chlorophyll suspension solution

Reflectance spectrum of paint

ISR-240A Integrat ing Sphere Attachment, 60mm dia.

This attachment is used for measurement of diffuse, and total and reflectance and
measurement of transmittance of liquids and solids.
Wavelength range: 240-800nm.
Integrating sphere: 60mm inner diameter, equipped with a photomultiplier.
Maximum size of reflection sample: 40~70mm wide, 10mm thick or 70~70mm
wide, 5mm thick.
Two BaSO4 white plates, two sample holders and BaSO4 powder (500g) are
included.
Incident angle: 0 deg.

Reference cell for transmission

measurement

Sample cell for transmission

measurement
Reference beam

Sample

(P / N 206-2 38 60 -91 )
(U V -2400/2 50 0 s er ie s, 3600 onl y)

BaSO4 white plate

Detector
(mounted
at the bottom)

Sample beam

Optical diagram

17

8. Suspensi o n a n d O p a q u e S a mp le M e a s u re me n t

Suspension and Opaque Samples

8 . Su s p e n s i o n a n d Op aq u e S a mple M easurement
ISR-2200 Integrating Sphere Attachment, 60mm dia.
(P /N 20 6-2 38 50 -91 )
(UV -24 00 /25 00 se r ies onl y)
This attachment is used for measurement of diffuse, total, and specular reflectance and
measurement of transmittance of liquids and solids. The accessory has a wide wavelength
range from 220 nm to 850 nm, and the following features :
Integrating sphere : 60 mm in inner diameter, equipped with a photomultiplier.
Maximum size of reflection sample : About 70 mm dia, and 12 mm thick.
Two BaSO4 white plates, two sample holders and BaSO4 powder (500g) are included.
Cells are not included.

ISR-3100 Integrating
Sphere Attachment, 60 mm dia.
(P /N 20 6-2 38 51 -91 )
(UV-360 0 only )

Reference cell
for transmission
measurement

Wavelength range: 220 ~ 2600 nm.

Integrating sphere: 60 mm in inner diameter, equipped with a
photomultiplier and a PbS cell.
Maximum size of reflection sample: About 100 mm dia.x15 mm thick.
Incident angle: 0 deg. / 8 deg.
Two BaSO4 white plates, two sample holders and BaSO4 powder
(500g) are included. Cells are not included.

Reference beam
Sample cell
for transmission
measurement
Sample beam

150mm dia. Integrating Sphere Attachment

LISR-2100 (P/N 206- 23862- 91)
LISR-3100 (P/N 206- 23862- 92)
(U V-3600 only)

Reference
beam

This attachment is used to measure the reflectance spectrum

of solid samples, such as powder, paper or cloth.
The integrating sphere attachment is used primarily to measure the
transmittance of solid or liquid samples. It is also suitable for color
analysis because stable measurement can be performed
regardless of the surface configuration of the samples.
Measurement wavelength range: 240~840nm (LISR-2100)
240~2400nm (LISR-3100)
Integrating sphere: inner diameter 150 mm
Reflectance sample installation space: about 150 (W) x 170 (H) x
30 (t) mm
Transmittance measurement accessory
(P/N 206-17270) is required.

18

Sample
beam
PM
PbS
(LISR-3100 only)

(solid)
Multipurpose Larg e-Sample Compar tments
MPC-2200 (P/N 2 06 - 23830- 91)
(U V -2 4 00 /25 00 se r ies onl y)

MPC-3100 (P/N 2 06 - 23831- 91)

(U V -3 6 00 only)
Reflection / transmission spectra of various solids such as a large disk, silicon
wafer, lens, plastics, and glass are easily obtainable.
Built-in 60 mm dia, integrating sphere permits measurement of a non-flat
surface sample (such as lens) or thick glass.
Wide wavelength range : 240 ~ 800 nm (MPC-2200)
240 ~ 2600 nm (MPC-3100)
Maximum sample size : Transmission : 305 mm dia. x 50 mm thick or
204 mm dia. x 300 mm long
Reflection : 305 mm dia. x 50 mm thick
Height-adjustable V stage is built-in.

Reflectance spectrum of a CD measured in a multi-purpose large-sample compartment

Attached mirror for

introducing beam

M1

R beam
M5

Main body of multi-purpose

large-sample compartment

S beam

M4

M3
M2

M6
Integrating
sphere

V stage
Multi-purpose large-sample compartment

19

8. Suspensi o n a n d O p a q u e S a mp le M e a s u re me n t

Suspension and Opaque Samples

8 . Su s p e n s i o n a n d Op aq u e S a mple M easurement
Specular Reflectance Attachment
5 incident angle ( P/ N 206- 14046)
Reference
sample

The technique of specular reflectance is often applied to the examination of

semiconductors, optical materials, multiple layers, etc. The 5 Kincident angle
minimizes the influence of polarized light. Thus, no polarizer is necessary.
Samples as large as 100W x 160D x 15Th mm can be readily measured
with the UVmini Series, UV-1600/1700/1800, Solidspec-3700, and MultiSpec-1500.
Samples as large as 140W x 160D x 10Th mm can be readily measured
with the UV-2400/2500 series, UV-2450PC/2500PC, and UV-3600.
Sample placement is easy -- just set it on the holder with the measuring
surface down.
Installed in the standard sample compartment.

Sample

Reflected light

Incoming light

Reflected light

(a) when set at 100%

Incoming light

(b) during measurement

Relative specular reflectance measurement

Note: 1. The Alternate Sample Compartment (P/N 206-60184-07) is

necessary for the UVmini-1240/1240V, and MultiSpec-1500.
2. Direct Detection Unit (See page 22) is necessary for the
SolidSpec-3700/3700DUV.

Absolute Specular Reflectance Attachment

to detector

(U V -2 4 00 /25 00 se r ies, 3600, Sol i dSpec onl y)

These accessories are intended for use with the MPC-2200 / 3100 or Solid
Spec-3700 sampling compartment and require a Sample Base Plate
Integrating Sphere Set (See page 21). At angles of incidence greater than
30 degrees, a polarizer is also required.
V-N method : Optical path is easily switched between 100% level setting
and sample measurement.
Approximate sample size : 25 to 200 mm dia, or 20 to 150 mm square,
up to 30 mm thick.

to detector

Incoming light

Incoming light

Sample

(a) when set at 100%

(b) during measurement

Absolute specular reflectance measurement

ASR Attachment

P/N

Incidence Angle

ASR 3105

206-16817

ASR 3112

206-16100

12

ASR 3130

206-15001

30

ASR 3145

206-15002

45

Range
300 ~ 800nm (MPC-2200)
300 ~ 2400nm (MPC-3100)
300 ~ 800nm (MPC-2200)

Ref
100%light M2

300 ~ 2500nm (MPC-3100)

Measuring light

300 ~ 800nm (MPC-2200)

300 ~ 2300nm (MPC-3100)
300 ~ 800nm (MPC-2200)

M2'

300 ~ 2300nm (MPC-3100)

Polarizer position

M3
M1

Integrating
sphere
Sample
Configuration of absolute specular reflectance instrument

20

(solid)
Sample Base Plate Integrating Sphere Set
(U V -2400/2 50 0 s er ie s, 3600, Sol i dspec onl y)
This is used to install an absolute specular reflectance attachment to the UV main unit.

P/N

2400/2500
Series

3600

SolidSpec SolidSpec
-3700
-3700DUV

BIS-3100

206-17059

Yes

Yes

No

No

240 ~ 2600 nm

BIS-3700

206-20880-91

No

No

Yes

No

240 ~ 2600 nm

BIS-3700 DUV

206-20880-92

No

No

No

Yes

175 ~ 2600 nm

Range

Larg e Polariz er Set ( P/ N 206- 15694)

Polariz er Type1 (P/ N 206- 13236- 01)
Polariz er Type2 (P/ N 206- 13236- 02)
Polariz er Type3 (P/ N 206- 13163)
Polariz er Adaptor Set ( P/ N 206- 15693)
(U V -2400/25 00 se r ies , 3600, Sol i d Spec onl y)
This attachment is used to obtain accurate reflectance measurements at high angles
of incidence.
A polarizer adaptor set (P/N 206-15693) is a mounting accessory for polarizers.
Note: This polarizer can only be used in multipurpose large-scale sample
compartments and absolute specular reflectance attachments.
P/N

Effective Diameter

Range

Large Polarizer Set

206-15694

20 mm

250~2500 nm

BPolarizer Type1

206-13236-01

18 mm

400~800 nm

BPolarizer Type2

206-13236-02

17 mm

260~700 nm

BPolarizer Type3

206-13163

10 mm

260~2500 nm

21

9. Speci al Ac c e s s o r i e s f o r S o lid Sp e c - 3 7 0 0 /3 7 0 0 DUV

Suspension and Opaque Samples

9 . S p e c i a l A cc e s s o ries fo r S o lidSpec- 3700/3700DUV
Automatic X-Y Stag e

( P/ N 206- 20810- 39)

The Automatic X-Y Stage enables automatic measurements for the points specified in
advance and is a powerful accessory for high-throughput measurements.
Maximum sample size : 310mm diameter or 310 x 310mm, 40mm thickness

Direct Detection Unit DDU

(P /N 20 6-2 02 64 -91 )

Direct Detection Unit DDU-DUV

(P /N 20 6-2 02 64 -92 )
The same sample compartment as a conventional UV-VIS spectrophotometer can be
added to the SolidSpec-3700/3700DUV by mounting the Direct Detection Unit DDU or
DDU-DUV.
Measurement wavelength range :
DDU 190 ~ 3300nm (when mounted in SolidSpec-3700)
DDU-DUV 165 ~ 3300nm (when mounted in SolidSpec-3700DUV)

Purg e Box

(P/N 2 06- 21788- 91)

The Purge Box is used with the Direct Detection Unit DDU-DUV, allowing the inside to
be purged. The Purge Box has a film holder and a six-cell holder and allows the cell
positions to be moved without opening the cover of the SolidSpec-3700/3700DUV.
Maximum sample size :
60 x 60mm, 20mm thickness

Larg e Specular Reflectance Attachment

(5 incident angle)
(P /N 20 6-2 05 70 -91 )
The Large Specular Reflectance Attachment is needed for relative specular reflectance
measurements. This accessory is mounted in the main body of SolidSpec-3700/3700DUV
and enables reflectance measurements keeping the samples horizontal. The Direct
Detection Unit DDU or DDU-DUV is not required for this accessory.
Applicable sample size: maximum 470W x 560D x 40H (mm)

Square Cell Holder for Integrating Sphere

(P /N 20 6-2 23 39 -92 )
10mm square cell holder for integrating sphere built into SolidSpec-3700/3700DUV systems.

22

10. P r i nter

(solid)
1 0 . P ri n t e r
DPU-414 Screen Copy Printer
(P / N 206-5 52 15 -* * ) (UVm i ni - 1240, UV- 1700 onl y)
(P / N 206-5 52 10 -* * ) (UV- 1601 onl y)

MPU Screen Copy Printer

(P / N 206-2 60 07 -* * ) (UV1800 onl y)
Prints hard copies of screens, including numeric data. A printout is made after each
measurement.
Spectra, kinetics reaction data, and quantitation calibration curves displayed on the screen
are output in the screen print. A hard copy can be printed at any time, making it simple to
record measurement parameters.
The printer cable is included.
Thermal paper (10 rolls) : (P/N 088-58907-04)

DPU-414 Dimensions: 160W x 170D x 66.5H (mm)

MPU Dimensions: 170W x 155D x 88H (mm)

23

11. I nter faces , C a b l e

Suspension and Opaque Samples (solid)

1 1 . I n t e rf a c e s, C ab le
Analog Signal Output Interface
(P / N 2 06 -25 23 3-9 1)( UV- 1800 onl y)
Used to record output of the UV-1800 on an analog recorder.
Analog output full scale : 100 mV/2 Abs. or 100 mV/100%T

Interface cable (Centronics standard)

(UV mini s er ie s, UV- 1600/ 1700 onl y)
Used to print with a commercial printer (ESC/P only).
Note: Even with a color printer, output is only in monochrome.

USB Interface Cable

( P/ N 088- 52848- 32)

(U V -1 80 0 only )
Used to connect the UV-1800 to the PC.

RS-232C Cable (Type 2)

( P/ N 200- 86408)
(U V mini Se r ies , UV- 1601, UV- 1700 onl y)
Used to connect a UVmini Series, UV-1601, or UV-1700 to an IBM
compatible PC. The connection is made as shown to the figure below.

D-sub 9-pin female connector

on personal computer

The cable has a 9-pin female connector on the PC side, and a 9-pin
male connector on the spectrophotometer side.
Only three lines are used for communication: two lines for signal
output and input and one for grounding.
The pins of the control lines on the PC side are connected so that
signal input and output are always ready to be made from the PC side.

RXD

TXD

TXD

RXD

DTR

GND

Signal GND

DSR

RTS

CTS

8
9

24

D-sub 9-pin male connector

on spectrophotometer

12. S oftw ar e

Software
1 2 . S o f t w a re
UVProbe Ag ent software ( P/ N 206- 21550- 92)
(U V -1650PC/1 80 0/2 45 0/ 2550/ 3600/ Sol i dSpec onl y)
The UVProbe Agent is a unique tool to manage various types of data acquired by
the UVProbe for quick and easy data retrieval for further study, approval, browsing,
back-up and disposal thereafter.
The CLASS-Agent supports FDA 21 CFR Part 11 (Electronic Records and
Electronic Signatures) and data security management and electronic signature
functions are incorporated for data stored in the database.
Data acquired by the UVProbe are automatically transferred and stored in the
database, which can be easily queried.
Spectra and peak detection tables included in the data stored in the database can
be directly browsed.
The UVProbe Agent is network-compatible. By installing an Agent software for
corresponding analytical instruments, such as HPLC or FTIR spectrophotometer,
data from all analytical instruments can be integrally managed at a server PC and
those data can be browsed at client PCs.
OS: WindowsXP/Professional SP2/Vista Business.
Note:

UVProbe software Ver. 2.00 or higher is required.

Data acquired/processed by Kinetics module is not compatible.

Performance Validation Software

( P/ N 206- 8 4 8 3 6 - 9 1 )

(U V -1650PC/2 45 0/2 55 0 onl y)

Routine checking, calibration, and quality recording of your instrument performance are quite important for maintaining the reliability of data given
by the instrument.
The Shimadzu Performance Validation Software carries out all these jobs with minimized labor and with minimal operator errors.

Features
Test results are printed out automatically together with the time and date.
Automate the number and sequence of up to 11 test validation methods. This batch of methods becomes your Standard Operating Procedure.
The results, conditions, and history of checking may be automatically filed.
This software runs on Windows XP/2000.

Specif ications
Checking is made on the following 11 items, and the results are printed out in a semi-automated sequence. (The installation and removal of
standard samples and/or tools for checking are made manually.)
Instrument status after initialization
Wavelength accuracy
Wavelength repeatability
Wavelength resolution
Stray light
Photometric accuracy
Photometric (measurement) repeatability
Baseline stability (amount of drift)
Noise level
0% T line flatness

25

12. S oftw ar e

Software
1 2 . So f t w a re
Film Thickness Measurement Software

Color Measurement Software

(P /N 20 6-6 68 77 )
(UV -16 01 PC/24 01 PC/ 3101PC/ 3600/ Sol i dSpec only )

(P/N 206-67449)
( U V - 1 6 0 1 P C / 2 4 0 1 P C / 3 1 0 1 P C / 3 6 0 0 / S o l i d S p e c only )

This software provides calculation of film thickness from the peak

positions of the interference pattern.

This software calculates color indices from the spectra given by the
spectrophotometer.

Measurable thickness range is expressed by the next equation,

though it somewhat differs with the types of samples.
e
50s
<d<
n
n
e: Shortest measured wavelength
s: Longest measured wavelength
n: Refractive index of sample
Automatically detects valleys and peaks of interference spectrum.

Presents indices of XYZ, Lab (Hunter), L*a*b*, L*u*v*, Munsell,

whiteness, yellowness, metamerism, and many others.
Recalculates any results with different parameters and conditions.
Chromaticity diagram and magnified color difference diagram may
be graphically displayed.
Permits computation using user-defined illuminants, besides the
standard ones. The user-defined illuminants may be stored as files
to be recalled at any time.

Calculates film thickness from the wavelengths of all the peaks and
valleys within the specified wavelength range.

Correction using the standard white plate ensures high accuracy in

color computation. The set standard values may be stored as files
to be recalled at any time.

Wavelength range may be selected watching the interference

pattern.

Calculates color differences using the arbitrarily selected standard

samples.

This software runs on Windows XP/Professional SP2/Vista

Business.

Available convenient functions include thickness correction,

smoothing, averaging, and standard deviation calculation.

Note: This software does not include a RS-232C connection cable.

For use with the UV-1601, either UVPC software (206-6057004) or a RS-232C cable (200-86408:IBM PC compatible) should
be used.

Up to 100 data may be displayed together.

This software runs on Windows XP/Professional SP2/Vista
Business.
Note: This software does not include a RS-232C connection cable.
For use with the UV-1601, either UVPC software (206-6057004) or a RS-232C cable (200-86408:IBM PC compatible) should
be used.

DLT- UVPC Solar Transmittance Measurement Software

(P / N 20 6-2 31 30 -92 )
(U V -3 1 50 /36 00 , SolidSpec- 3700/ 3700DUV onl y)
This software calculates the solar transmittance, solar reflectance, visible radiation transmittance, and visible light reflectance for sheet glass and films.
Users can create their own table of weighting factors and can save
weighting coefficient tables as a file.
Results can be recalculated using a different reference sample.
Users can create their own light illumination for color calculations.
Users can save the light illumination they create as a file.
Highly precise calculation results can be obtained using white
tile correction. Reflectance data from the white tile can be saved
as a file.

26

Chromaticity coordinates can be graphed.

Compatible Operating Systems: Windows XP/Professional
SP2/Vista Business.
Note: Using UV-PC to operate a UV-3100PC system in Windows
3.1/95/98 requires upgrading to Windows XP/2000 and UVProbe
software (P/N 206-21440-91).

Optional Software for UVmini-1240/1240V

Multiwavelength Measurement Program Pack
(P /N 206-8 97 55 -92 )
This multiwavelength measurement program pack can measure up to six wavelengths.
It can also simultaneously display the difference and ratio for two wavelengths in response
to measured absorbances or transmittance rates as well as calculation results for threewavelength calculation. It permits linked operation with the optional CPS cell positioner
to allow up to 6 samples to be measured concurrently.
The wavelength program can be set for up to six wavelengths.
Measuring data can be selected from two modes: absorbances or transmittances.
Photometric values can be used for producing calculations.
1) Ratio and difference of photometric values for two wavelengths
2) Three-wavelength computation
3) Four data formula computation:
(K1 x A1+K2 x A2+K3 x A3+K4 x A4) x K5
4) Four data formula computation:
K5 x (K1 x A1+K2 x A2) / (K3 x A3+K4 x A4)
An (n = 1 to 4) is the absorbance at measuring wavelength ln (n = 1 to 4).
The same settings are possible for transmittance rates.

Measuring Screen for Multiwavelength Measurement Pack

Measurement results are printed out for each measurement.

Sample exchange for each wavelength:
When one measurement is made, the sample can be exchanged for each wavelength
so that the measurement can be taken.

Calculation Formula Setting Screen for

Multiwavelength Measurement Pack

Kinetics Program Pack

(P /N 206-8 97 56 -92 )
This software is used for measuring time change in absorbance at a constant wavelength
and calculating enzyme activity values or other types of values.
Calculation and recalculation of the activity value is possible through linear regression using
the least-squares method.
The coefficients used in the activity value calculation can be set to a maximum of
four types.
The setting range for measuring is from 1 to 6550 seconds or minutes.
Measuring of two wavelengths is possible. Absorbance time change can be recorded while
absorbance at the background wavelength is being extracted from absorbance at the
measured wavelength.
Data processing function for reaction curves:
Expansion and compression (Note that compression is possible only in the vertical axis.)
Data readout with the cursor key, Reaction curve storing and recall

Activity Value Display Screen for Kinetics Program Pack

Measurement results (chart data) can be stored and recalled.

Note: Synchronized measurement with multiple cells such as CPS-240A is not possible.

Time Scan Program Pack

(P /N 206-8 97 57 -92 )
The time scan program pack can record the time change for photometric values
(transmittance rates, absorbances and energy) at a constant wavelength. The state of
change is displayed on screen as a time scan curve.
Measuring data can be selected from three modes:
absorbance, transmittance rate and energy.
The setting range for measuring is from 1 to 6550 seconds or minutes.
Data processing function for reaction curves:
Expansion and compression (Note that compression is possible only in the vertical axis.)
Data readout with the cursor key, Reaction curve storing and recall

27

Calculation Formula Setting Screen for

Multiwavelength Measurement Pack

Protein Analysis Program Pack

(P / N 20 6-8 97 58 -92 )
The protein analysis program pack is a single package that combines four
quantitative methods for measuring protein concentrations using coloring
reagents and a quantitative method for direct measurement of concentrations
calculated from measured absorbances and absorption constants that have
been set in advance.
Quantitation Methods
Lowry method, BCA method (method using Bicinchoninic Acid)
CBB method (method using Coomassie Brilliant Blue G-250)
Biuret method, UV absorption method (280 nm)
The calibration curve function is the same as the standard quantitation mode.
(function using calorimetric method)

Condition Setting Screen (Lowry Method) for

Protein Analysis Program Pack

Quantitation is possible from repeated measuring (1 to 10 measurements)

together with those mean values.
Measurement results (chart data) can be stored and recalled.

Water Analysis Program Pack

(P / N 20 6-8 97 51 -92 )
Easy and accurate water analysis can be conducted in combination with
simplified reagents.
The number of analysis items are 48 items in 31 types, and all the analysis
conditions are installed. Just select an item (including measurement of wavelength, calibration curve, measuring time, and measurement concentration
range for each individual item) and it will be set automatically.

Item Selection Screen for Water Analysis

Program Pack

Results can be acquired even without analytical knowledge through operation in accordance with screen instructions. The pack comes with an analysis
guide which displays the number of the reagent to be used and the operation
procedure, so there is no need to refer to the manual.
If the optional multicell holder (6-cells) is used, up to six cells can be measured consecutively in one analysis.
Automatic analysis commences after specified time. Elapsed time is displayed on screen, concentration values are displayed automatically after the
specified time has elapsed, and a buzzer sounds to state that analysis is
completed.

Operation Screen for Water Analysis Program Pack

List of Measurable Items

Chem ica l s y mbo
Al
Ba
Ca
Cl
CIO
CN
CO D
Col or
Cr
Cu
DO
F
Fe

FO R
K
Mg
Mn
NH 4
Ni

Mea s u r a b l e i te m
A lu min u m
B a riu m
Calc iu m
Chlo rid e
Res id u a l c h lo rin e : DP D me t h o d
Cya n o g e n : T o t a l c y a n o g e n
Che mic a l o x y g e n d e ma n d
Colo r
Chro miu m: He x a v a le n t c h ro miu m
Chro miu m: T o t a l c h ro miu m
Cop p e r
Dis s o lv e d o x y g e n
Flu o rin e
Iro n (F e rru m): Div a le n t iro n
Iro n (F e rru m): Div a le n t iro n a t lo w c o n c e n t ra t i o n
Iro n (F e rru m): T o t a l iro n (re d u c in g me t h o d )
Iro n (F e rru m): T o t a l iro n a t lo w c o n c e n t ra t io n
For ma ld e h y d e
P o t a s s iu m
Mag n e s iu m
Man g a n e s e
A mmo n iu m
A mmo n iu m n it ro g e n
Nic k e l

Chemical symbo
NO2
NO3

Pb
pH

Phenol
PO4
SiO2
SO3
SO4
maldehyde
Turbid.
Zn

Measurable item
Nitrite
Nitrite-nitrogen
Nitrate: Nitrate free
Nitrate: Nitrate in 0.2 mg/l max.
Nitrate: Nitrate in 0.2 mg/l min.
Nitrate-nitrogen: Nitrite-nitrogen free
Nitrate-nitrogen: Nitrite-nitrogen in 0.06 mg/l max.
Nitrate-nitrogen: Nitrite-nitrogen in 0.06 mg/l min.
Lead: Not including other metals
Lead: Including other metals (KCN used)
BCG
CPR
BTB
CRb
Phenol
Phosphate
Silica: High concentration
Silica: Low concentration
Sulfite
Sulfate
TH Total hardness
Turbidity
Zinc: Not including other metals
Zinc: Including other metals (KCN used)

28

12. S oftw ar e

Software
DNA/Protein Program Pack
(P / N 2 06 -89 75 0-9 2)
The concentration of DNA and protein is quickly calculated by using an
optional program pack. The measurement wavelengths and the calculation
are preprogrammed. Quantitative results are easily measured by simply
setting the sample into the spectrophotometer and pressing START.
Absorbance ratios and DNA/Protein calculations are readily available in the
standard menu and the measurement wavelengths and factors can be
modified to match your specific requirements.
Concentration calculating formula is selectable between two types as below.
1) A1 = Absorbance at 260nm A2 = Absorbance at 230nm
Ratio = A1/A2
DNA concentration = 49.1 x A1 - 3.48 x A2
Protein concentration = 183.0 x A2 - 75.8 x A1
2) A1 = Absorbance at 260nm A2 = Absorbance at 280nm
Ratio = A1/A2
DNA concentration = 62.9 x A1 - 36.0 x A2
Protein concentration = 1552.0 x A2 - 757.3 x A1
Selectable background correction for the absorbance at 320nm is available.

Measurement display for DNA/Protein Program Pack

Note: Not applicable to UVmini-1240V.

UV Data Manag er Software

(P / N 20 6-8 97 65 -92 )
With the UV Mini connected to a PC via the standard RS-232C port, the UV
Data Manager helps organize and store data files in memory of a computer,
the spectrophotometer or the data packs.
Some of the features of the software include:
Management of data and parameter files.
Concentration calculating formula is selectable between two types
of formula.
Data translation from the spectrophotometer to the PC into a text file
for easy pasting into a spreadsheet software package.
Download previously saved data from the PC to the spectrophotometer for expanded storage and backup capabilities. Data can also be
uploaded onto the IC data pack cards for multiple instrument users.
Convenient sorting and searching possibilities for files in the data
list box of the spectrophotometer, helping to maintain order of your
essential information.
Spectral data can be graphically displayed in the UV Data Manager
for quick and easy data interpretation.
Note: UV Data Manager is to be operated under MS-Windows 95/98/NT/2000/XP.
Use IBM-PC compatible personal computer.
Use Shimadzu RS-232C interface cable. The part number is 200-86408.

Measurement display for DNA/Protein Program Pack

Support files:
Photometric files
Spectrum files
Quantitation files
*Optional program pack files are not
available.

Data Pack
(P / N 20 6-8 07 00 )
Stores 46 files of experimental parameters, or 21 files of time-course and/or spectral curves.
Note: This Data Pack is identical to the UVmini-1240 Series Data Pack but the files are not compatible.
The Data Pack must be initialized for use with the UV-1700.

29

Shimadzu UV-VIS spectrophotometers

Minimum
Bandwidth
(nm)
SolidSpec-3700DUV

SolidSpec-3700
0.1
UV-3600

UV-2450 / 2550

UV-1800

UVmini-1240 / BioSpec-mini

165

190

900

1100

3200 3300
Wavelength Range (nm)

JQA-0376

Founded in 1875, Shimadzu Corporation, a leader in the

development of advanced technologies, has a distinguished
history of innovation built on the foundation of contributing to
society through science and technology. We maintain a global
network of sales, service, technical support and applications
centers on six continents, and have established long-term
relationships with a host of highly trained distributors located
in over 100 countries. For information about Shimadzu, and to
contact your local office, please visit our Web site at

www.shimadzu.com

SHIMADZU CORPORATION. International Marketing Division

3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, Japan
Phone: 81(3)3219-5641 Fax. 81(3)3219-5710

URL http://www.shimadzu.com

The contents of this brochure are subject to change without notice.

Printed in Japan 3295-10010-50ANS