INNOVATIVE PRODUCTS

Stationary phase comparison.

Resteks Exclusive Trident Integral System
Alkyl phases (e.g. C18) Convenient and economical leak-free guard cartridge system, extremely easy to install.
Versatile configuration protects against all levels of contamination.

HPLC Column
Si Alkyl-based stationary phases, such
as C18, are best suited for analyzing Integral design eliminates troublesome tubing connections.
C18 hydrophobic molecules with a high
Si carbon:heteroatom ratio. Description qty. cat.#
Silica Surface

XG-XF Fitting for 10mm Guard Cartridge ea. 25026

XG-XF Fitting for 20mm Guard Cartridge ea. 25062
Si Si Replacement XF Filter Fitting ea. 25024

Selection Guide
Replacement Cap Frits: 4mm, 2.0m 5-pk. 25022
Replacement Cap Frits: 4mm, 0.5m 5-pk. 25023
Si Replacement Cap Frits: 2mm, 2.0m 5-pk. 25057
Si
Alkyl phase with polar
Si
P3 P=Polar Group functional group Please see Resteks Annual
An alkyl-based stationary phase Chromatography
Si
Silica Surface

with either an embedded polar Products Guide (lit. cat.#

group (P1), a polar side chain (P2), XF End Cap XG-XF Guard End 580205) for more HPLC
Fitting Frit Fitting Cartridge Fitting columns and accessories.
P2 or a polar end-cap (P3), has
Si significantly greater interaction with Column with Trident Integral Inlet Fitting (to order add -700 to catalog number of column)
Si P1 polar compounds than a traditional and guard cartridge, XG-XF fitting, cap frit, and XF end fitting.
alkyl phase.
Si

Remove the XF end fitting Add the XG-XF fitting Re-install the XF end The cap frit can be easily
Conventional Biphenyl
and install the guard (order cat.# 25026 for fitting with cap frit. replaced if it becomes
Propyl Phenyl Phenyl & Biphenyl phases cartridge in the end of 10mm guard cartidges, contaminated/plugged.
Phenyl stationary phases interact the column. cat.# 25062 for 20mm
with compounds containing guard cartridges). HPLC Products Catalog
aromatic groups or unsaturated (lit. cat.# 58059241C)
bonds through - interactions.
The biphenyl stationary phase has
even greater interaction due to the
higher concentration of aromatic
Si Si rings. Restek Trademarks:
Allure, pHidelity, Pinnacle, Restek logo.
Si Si Si Si Si Si Si Si Si Si
Other Trademarks:
Silica Surface Silica Surface Zorbax (Agilent Technologies, Inc.), Kromasil (Eka Chemicals), Intersil (GL Sciences, Inc.), LiChrospher (Merck KGAA), Develosil (Nomura Chemical Co.), AQUA, Jupiter, Luna (Phenomenex, Inc.),
Discovery (Sigma-Aldrich Co.), Betamax, Betasil, BioBasic, Fluophase, Hypersil, Prism (Thermo Scientific), Spherisorb, Symmetry, YMC (Waters Corp.)

F F
Pentafluorophenyl
Propyl
N Cyano
Cyano & Fluorinated phases
Fluorinated phases, such as the
Restek Corporation
110 Benner Circle
Presorted Standard
US Postage
New
F F C
pentafluorophenyl propyl (PFP Bellefonte, PA 16823-8812 PAID 1.9m Pinnacle DB
propyl), and cyano-based phases Restek
interact strongly with basic, HPLC Columns!
nitrogen-containing and
halogenated analytes.

Si Si
Lit. Cat.# 59454D
Si Si Si Si 2008 Restek Corporation.

Restek U.S. 110 Benner Circle Bellefonte, PA 16823 814-353-1300 800-356-1688 fax: 814-353-1309 www.restek.com
Restek France phone: 33 (0)1 60 78 32 10 fax: 33 (0)1 60 78 70 90 e-mail: restek@restekfrance.fr
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Thames Restek U.K. LTD phone: 44 1494 563377 fax: 44 1494 564990 e-mail: sales@thamesrestek.co.uk
Restek GmBH phone: +49 (0) 6172 2797 0 fax: +49 (0) 6172 2797 77 e-mail: info@restekgmbh.de www.restek.com 800-356-1688 814-353-1300
 INNOVATIVE PRODUCTS

HPLC Column Selection Guide

Stationary phase selection

Water or Sample Solubility Nonpolar

Polar Organics Organics

Reversed Phase Normal Phase

Ionic Neutral Mixed

Acidic Basic Hydrophobic Hydrophilic

Aqueous Amino Biphenyl

C1 Aqueous Basix Amino
C18 Basix C4
C18 C18 IBD Basix
IBD Cyano C8
Phenyl IBD Cyano
Organic IBD Organic IBD
Acids PFP Acids PFP Propyl
PFP Propyl Silica

Restek End Pore Carbon USP

HPLC Column Cap? Size () load (%) Applications Chromatographic Properties Similar Phases Code
Hypersil BDS C18, Zorbax
Hydrophobic C18 phase suitable for analyses of a wide Highly base-deactivated spherical silica manufactured by Restek.
Pinnacle DB C18 Y 140 11 Eclipse XDB-C18, Spherisorb L1
range of compounds, from acidic through slightly basic. Monomeric C18 bonding.
ODS
Aquasil C18, AQUA C18,
Pinnacle DB Ideal for applications that require highly aqueous mobile Highly selective phase for polar analytes. Compatible with highly
140 6 Hypersil Gold AQ, YMC L1
Aqueous C18 phases, such as organic acids and water-soluble vitamins. aqueous (up to 100%) mobile phases. Silica manufactured by Restek.
ODS-Aq
Applications similar to Pinnacle DB C18, but with less Highly base-deactivated spherical silica manufactured by Restek.
Hypersil BDS C8,
Pinnacle DB C8 Y 140 6 hydrophobic retention. Less retention can be useful Monomeric C8 bonding. Similar to Pinnacle DB C18, but the shorter L7
Spherisorb C8
for shortening analysis time, if resolution is adequate. alkyl chain provides less hydrophobic retention.
Exhibits excellent peak shapes for a wide range of
Pinnacle DB PFP Highly base-deactivated spherical silica manufactured by Restek.
Y 140 6 compounds, including nucleosides, nucleotides, Discovery HS F5 L43
Propyl Unique pentafluorophenyl phase with a propyl spacer.
and halogenated compounds.
Highly base-deactivated spherical silica manufactured by Restek.
Excellent choice for the analysis of steroids, tetracyclines,
Pinnacle DB Unique reversed phase material that displays both increased retention
Biphenyl
Y 140 8 drug metabolites, and other compounds that contain
and selectivity for aromatic and/or unsaturated compounds when Unique L11
some degree of unsaturation.
compared to conventional alkyl and phenyl phases.
Suitable for a wide range of compounds, from acidic
Hypersil BDS Cyano,
Pinnacle DB through slightly basic. Also useful for confirmation of Highly base-deactivated spherical silica manufactured by Restek.
Y 140 4 Spherisorb Cyano, L10
Cyano analyses on a C18 or C8 column. Can be used in normal Cyano bonding.
Zorbax Eclipse XDB-CN
phase or reversed phase mode of separation.
Hypersil BDS Phenyl,
Pinnacle DB Suitable for polar aromatic compounds, fatty acids, Highly base-deactivated spherical silica manufactured by Restek.
Y 140 5.3 Spherisorb Phenyl L11
Phenyl purines and pyrimidines. Phenyl bonding.
Zorbax Eclipse XDB-Phenyl
Pinnacle DB Silica 140 Normal phase mode of separation. Highly base-deactivated spherical silica manufactured by Restek. L3

Intermediate carbon load and surface area, suitable for a wide range of
Pinnacle II C18 Y 110 13 Superior general purpose C18 for nonbasic analytes. Hypersil ODS L1
neutral to acidic compounds. Silica manufactured by Restek.
Proprietary stationary phase; resolves 16 PAHs in US EPA Method 610.
Pinnacle II PAH Y 110 Maximum resolution of polycyclic aromatic hydrocarbons.
Silica manufactured by Restek. Unique

Provides shorter retention times for hydrophobic compounds than C18.

Pinnacle II C8 Y 110 7 Superior general purpose C8 for nonbasic analytes. Hypersil C8 L7
Silica manufactured by Restek.
Superior general purpose cyano for weakly basic analytes. More rugged than bare silica for normal phase analyses.
Pinnacle II Cyano Y 110 4 Hypersil CPS L10
Used in either normal or reversed phase analyses. Silica manufactured by Restek.
Offers unique selectivity versus traditional alkyl chain phases,
Pinnacle II Phenyl Y 110 6 Superior general purpose phenyl for neutral analytes. Hypersil Phenyl L11
especially for aromatic compounds. Silica manufactured by Restek.
Excellent general purpose amino phase. Hypersil APS 2 Amino,
Pinnacle II Amino N 110 2 Silica manufactured by Restek. L8
Excellent choice for carbohydrate analysis. Spherisorb Amino
Pinnacle II Multiple aromatic ring structures;
Biphenyl
Y 110
excellent for explosives.
Silica manufactured by Restek. Unique biphenyl phase. Unique L11

Superior value phase for normal phase separation of polar analytes.

Pinnacle II Silica 110 Ideal for polar analytes. Hypersil Silica L3
Lower retention than Ultra C18. Silica manufactured by Restek.
Ideal for MS and light-scattering detection of neutral to
Most retentive phase for hydrophobic and slightly polar analytes.
slightly polar solutes. Separates basic compounds, Ultracarb C18, BetaMax
Allure C18 Y 60 27 Mobile phase containing higher percentage of organic modifier L1
showing good deactivation; excellent for explosives or Neutral, Discovery C18
contributes to higher sensitivity in ESI-based LC/MS.
steroids.
Ideal for analyses that require >90% water in the mobile
phase. Excellent for highly water soluble or poorly organic Highly retentive and selective for reversed phase separations of polar
Allure Aqueous
C18
N 60 soluble compounds. Excellent for water-soluble vitamins analytes. Highly base deactivated. Compatible with highly aqueous Unique L1
and organic acids. More retention than Ultra Aqueous (up to 100%) mobile phases.
columns.
Ideal for the analysis of aldehydes and ketones Highly retentive, highly selective phase, developed specifically for
Allure AK Y 60
as DNPH derivatives. the analysis of aldehydes and ketones as DNPH derivatives. Unique

Ideal for LC/MS of basic solutes. Excellent for basic

Allure Basix Y 60 12 Highly retentive phase for analytes containing amino functionality. BetaMax Base, Maxsil CN L10
pharmaceuticals or other amine-containing compounds.
Ideal for MS, ELSD, or NPD detection of nucleosides, A pentafluorophenyl phase with a propyl spacer. Highly retentive for
Allure PFP Propyl Y 60 17 nucleotides, purines, pyrimidines, or halogenated basic analytes. Excellent for beta-blockers, halogenated compounds, Discovery HS F5 L43
compounds. nucleosides, nucleotides, pyridines, pyrimidines, tricyclic antidepressants.
Allure Organic Single 30cm column performs equally to two C18 columns in series.
Acids
N 60 Excellent resolution of challenging organic acids.
(AOAC Method 986.13) Unique

Multiple ring structure; excellent for aromatic and

High purity, highly retentive phase for aromatic and unsaturated
Allure Biphenyl Y 60 23 unsaturated compounds. Increased retention over
compounds. Unique L11
traditional phenyl phases.
High purity, highly retentive phase for normal phase separation of
Allure Silica 60 Highly retentive phase for normal phase separation. Maxsil Si L3
polar analytes. Very high surface area.
Discovery C18, Symmetry
Ideal for anilines, barbiturates, carbonyls, fat-soluble A very retentive, high-purity phase that exhibits excellent peak shape C18, Hypersil Gold C18, Luna
Ultra C18 Y 100 20 vitamins, fatty acids, glycerides, phthalates, PTH amino for a wide range of compounds. Recommended as a general purpose C18, Zorbax C18, Kromasil L1
acids, steroids, other acids. reversed phase column. C18, LiChrospher RP-18,
Inertsil ODS-2, Develosil C18
Ideal for analyses that require >90% water in the
Highly retentive and selective for reversed phase separations of polar AQUA C18, Aquasil C18,
mobile phase. Excellent for highly water soluble or
Ultra Aqueous C18 N 100 15 analytes. Highly base deactivated. Compatible with highly aqueous Hypersil Gold AQ, YMC L1
poorly organic soluble compounds. Excellent for
(up to 100%) mobile phases. ODS-Aq
water-soluble vitamins and organic acids.
One of a group of intrinsically base-deactivated (IBD) phases, with a
A polar group assists in deactivating surface silanols and
polar group within, or intrinsic to, the alkyl bonded phase. Provides SymmetryShield, Discovery
Ultra IBD N 100 12 contributes to unique separation selectivities for acids,
unique selectivity and high level of base deactivation while reducing or ABZ & ABZ+, Prism
bases, zwitterions, and other polar compounds.
eliminating the need for mobile phase additives.
Selectivity and peak shape similar to Ultra C18, Very retentive, high-purity, base-deactivated reversed phase packing Luna C8, Symmetry C8,
Ultra C8 Y 100 12 L7
but less hydrophobic retention. that exhibits excellent peak shape for a wide range of compounds. Hypersil Gold C8
Exceptionally stable C4 packing, with high bonding coverage
Supelcosil Butyl (C4),
Ultra C4 Y 100 9 Ideal for peptides and small proteins. and silanol base-deactivation. Exhibits shorter retention than C18 L26
Delta-Pak C4
or C8 phases.
Alternative selectivity to Ultra C18 or C8 columns, Exceptionally stable C1 packing resists hydrolysis, even under
Ultra C1 100 5 especially for polar analytes. Shortest chain alkyl acidic mobile phase conditions. Least retentive reversed phase Spherisorb C1 L13
phase available for reversed phase separations. hydrocarbon packing.
High-purity cyano phase with reduced silanol activity. Often a better
Excellent for basic pharmaceuticals, steroids (normal or Platinum CN, Develosil Cyano,
Ultra Cyano Y 100 8 choice than C18 for basic pharmaceuticals. Cyano is the most stable L10
reversed phase conditions), or other basic compounds. Luna CN, Hypersil Gold CN
bonded phase for normal phase mode.
Ideal for fatty acids, polycyclic aromatic hydrocarbons, High-purity, highly retentive, base-deactivated phase with alternate Platinum Phenyl, Supelcosil
Ultra Phenyl Y 100 10 L11
purines and pyrimidines, and polar aromatics. selectivity to hydrocarbon phases, especially for aromatic analytes. Phenyl, Betasil Phenyl
Recommended for normal phase analyses of mono- and disaccharides
Superior general purpose amino phase. Ideal for Platinum Amino, Develosil
Ultra Amino N 100 2 and other similar compounds. Can also serve as a weak anion L8
carbohydrates. NH2
exchanger with aqueous buffers.
Ideal for taxol and precursors, or halogenated A pentafluorophenyl phase. Unique selectivity by interaction with Fluophase PFP, Fluosep-RP
Ultra PFP Y 100 7 L43
compounds, amines, esters, or ketones. functional groups of organohalogens or other basic analytes. Phenyl, Curosil PFP

Ultra Silica 100 Ideal for normal phase applications. High purity, high surface area. L3

Proprietary stationary phase can process up to twice as many

Ultra Carbamate 100 Rapid analysis of carbamates.
samples per hour, compared to a conventional C18 phase. Unique

Proprietary phase for the analysis of paraquat and High purity silica. Reversed phase packing optimized for the analysis of
Ultra Quat 100
diquat and other quaternary amines. paraquat and diquat. Unique

BioBasic 18, Symmetry 300

Silica manufactured by Restek. Viva wide pore silica has the highest avail-
C18, Jupiter 300 C18, Zorbax
Viva Wide Pore C18 Y 300 9 Proteins and other higher molecular weight compounds. able surface area for large molecules of any commercially available 300 L1
300 OSB C18, Synchropak C18,
packing.
208 TP C18
Proteins and other higher molecular weight compounds. Silica manufactured by Restek. Similar to Viva C18 with less hydrophobic BioBasic 8, Zorbax 300 OSB
Viva Wide Pore C8 Y 300 5 L7
Less retentive than C18 phase. retention. C8, Synchropak C8, 208 TP C8
BioBasic 4, Symmetry 300 C4,
Proteins and other higher molecular weight compounds. Silica manufactured by Restek. Similar to Viva C18 and C8, with even less
Viva Wide Pore C4 Y 300 3.5 Jupiter 300 C4, Synchropak L26
Less retentive than C18 and C8 phases. hydrophobic retention.
C4, 208 TP C4
Exhibits excellent peak shape for a wide range of
Viva Wide Pore
Biphenyl
Y 300 6.7 compounds; ideal for large molecules and biomolecules Silica manufactured by Restek. Unique biphenyl phase. Unique L11
with aromatic rings or unsaturated character.
Exhibits excellent peak shape for a wide range of
Viva Wide Pore Silica manufactured by Restek. Fluorinated phase for enhanced retention
PFP Propyl
Y 300 5 compounds, including nucleosides, nucleotides,
of basic & halogenated compounds. Unique L43
and halogenated compounds.
Silica manufactured by Restek. Viva wide pore silica has the highest avail-
Viva Wide Pore Normal phase applications for high molecular weight
300 able surface area for large molecules of any commercially available 300 L3
Silica compounds.
packing.
Hydrophobic C18 phase suitable for analyzing a wide
Excellent stability under extreme pH conditions. True C18 selectivity
pHidelity C18 140 range of compounds; enhanced stability under highly
in a silica-based stationary phase. Unique
basic conditions.

pH ranges and temperature limits: see www.restek.com.

Column lifetime will be shorter when operating at pH and/or temperature extremes.

Call Restek Technical Service, or your local distributor, for the right column for your application.

Chromatography Products
www.restek.com 800-356-1688 814-353-1300