CLINICAL BIOCHEMISTRY

MULTIPLE CHOICE QUESTIONS

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A QUICK RECAP OF ESSENTIAL POINTS FOR MEDICAL STUDENTS

PRACTICE SET BIOCHEM- II

100 SINGLE-OPTION MULTIPLE CHOICE TYPE QUESTIONS

Max. Questions: 100 BIOCHEMISTRY Max. Time: 75 mins
Basic Medical Sciences
SingleOption Multiple Choice Type Questions.
Choose the most appropriate correct answer from the options given below.
There is no negative marking for Wrong answers.

1. Acetoacetate:

a. brain cannot utilize acetoacetate

b. is synthesized from Beta-hydroxy butyrate
c. synthesized by the liver mitochondria
d. produced from transmination of tryptophan

2. Which of the following is NOT a lipotropic factor?

a. Choline
b. Leptin
c. Selenium
d. Methionine
e. Lecithin

3. Apoprotein apo B-48:

a. component of LDL
b. component of chylomicrons
c. produced by the liver
d. blood level is 100 mg/dl
e. component of HDL-2

4. CORRECT about 'Atkins diet' for obesity:

a. high fat, low carbohydrate, high protein

b. low fat, low carbohydrate, high protein
c. low fat, high carbohydrate, low protein
d. high fat, low carbohydrate, low protein
e. None of the above

5. Which of the following enzymes catalyzes the second step of beta-oxidation cycle?

a. Beta-hydroxyacyl-CoA dehydrogenase
b. acyl-CoA dehydrogenase
c. enoyl-CoA hydratase
d. acyl-CoA acetyltransferase
e. 3-Hydroxybutyrate dehydrogenase

6. Complete oxidation of 1 mol of palmitate via -oxidation produces

a. 129 mol of ATP

b. 121 mol of ATP
c. 133 mol of ATP
d. 147 mol of ATP

7. Xanthoproteic test is used for detection of

a. histidine
b. phenylalanine
c. arginine
d. cysteine

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8. Which of the following statements about brain metabolism is INCORRECT?

a. brain metabolizes 60% of total carbohydrate intake

b. brain utilizes ketone bodies during starvation
c. Pasteur effect protects brain aginst anoxia
d. brain can effectively utilize fatty acids

9. The color complex formed by amino acids when heated with ninhydrin

a. yellow
b. violet
c. purple
d. red
e. black

10. Apoprotein apo A-I:

a. component of LDL
b. inhibits LCAT
c. blood level is 150 mg/dl
d. blood level is 100 mg/dl
e. component of HDL-3

11. A magnesium containing enzyme:

a. Hexokinase
b. Lecithinase
c. carboxy peptidase
d. Enolase
e. Lipase

12. Oil having a highest content of PUFA:

a. corn oil
b. seasame oil
c. sunflower oil
d. cotton seed oil
e. gingelly oil

13. Regarding proteins at Iso-electric pH (pI):

a. will migrate faster in an electrical field

b. viscosity will be maximum
c. precipitation will be maximum
d. pI of human insulin is 6.4
e. pI of human hemoglobin is 4.7

14. Pauly's test for histidine gives what color?

a. pink
b. cherry-red
c. purple
d. red
e. dark-blue

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15. All of the following are nonessential aminoacids EXCEPT:

a. aspartic acid
b. alanine
c. leucine
d. serine
e. cysteine

16. A metallo-enzyme that contains copper:

a. Tyrosinase
b. Lecithinase
c. Peroxidase
d. Xanthine oxidase
e. Catalase

17. All of the following procedures can be used for protein precipitation EXCEPT:

a. Ingram's technique
b. Salting out
c. Iso-electric Precipitation
d. Alcohol
e. Heat coagulation

18. One of the following is diamino monocarboxylic acid:

a. proline
b. glycine
c. asparagine
d. lysine
e. glutamine

19. Which of the following inhibits enzyme Glucokinase?

a. insulin
b. glucose
c. fructose-6-phosphate
d. fructose-2, 6-bisphosphate

20. Which of the following enzymes belongs to Transferases?

a. Lactate dehydrogenase
b. Hexokinase
c. Aldolase
d. Acetyl CoA carboxylase

21. The precursor of Glutathione is:

a. glutamine
b. histidine
c. glutamic acid
d. glycine
e. formimino glutamic acid

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22. The site of action of lipoprotein lipase is:

a. adipocytes
b. capillary wall
c. small intestine
d. stomach
e. mouth

23. A WRONG match about one letter abbreviation of amino acids:

a. LysinezK
b. GlycinezQ
c. AsparaginezN
d. HistidinezD
e. PhenylalaninezF

24. Which of the following a nonessential amino acid?

a. methionine
b. lysine
c. phenylalanine
d. tyrosine
e. threonine

25. Rennin:

a. is a chymosin
b. secreted by the kidney
c. involved in fluid balance
d. deficiency may cause hypoaldosteronism
e. is an aspartyl protease

26. Which of the following is not a function of Phospholipids?

a. formation of triacylglycerols
b. absorption of lipids
c. membrane fluidity
d. formation of biomembranes of brain and muscles
e. emulsification of dietary lipids

27. Histones:

a. contain a high conc. basic amino acids

b. contain a high conc. acidic amino acids
c. histone synthesis starts when DNA synthesis ceases
d. are synthesized in the nucleus
e. are rich in hydroxyproline

28. An inhibitor of Glycogen phosphorylase:

a. insulin
b. glucagon
c. ATP
d. Acetyl CoA

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29. A WRONG match about one letter abbreviation of amino acids:

a. Asparagine zN
b. GlycinezQ
c. Tryoptophan zY
d. Lysine zK
e. Glutamic acidzE

30. Glucocorticoids are secreted from ____ of adrenal gland.

a. zona fasciculata
b. medulla
c. zona reticularis
d. zona glomerulosa

31. Globular proteins:

a. peptones
b. protamines
c. elastin
d. mucoproteins
e. histones

32. Least saturated:

a. coconut oil
b. seasame oil
c. ground nut oil
d. cotton seed oil
e. palm oil

33. Hormone sensitive lipase:

a. activated by insulin
b. inhibited by glucagon
c. increased in fed state
d. increased in starvation

34. Which of the following is the least dense of the blood lipoproteins

a. High-density lipoprotein (HDL)

b. Chylomicrons
c. Intermediate-density lipoprotein (IDL)
d. Very-low-density lipoprotein (VLDL)
e. Low-density lipoprotein (LDL)

35. A calcium containing enzyme:

a. Tyrosinase
b. Lecithinase
c. Enolase
d. Xanthine oxidase

36. Which of the following is a 19-carbon steroid?

a. progesterone
b. deoxycorticosterone
c. dehydroepiandrosterone
d. testosterone

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37. The site of action of hormone sensitive lipase

a. capillary wall
b. adipocyte
c. stomach
d. mouth
e. small intestine

38. INCORRECT about Phospholipase A2

a. site of action is small intestine

b. inhibited by steroids
c. site of action is capillary wall
d. precursor of arachidonic acid
e. activated by vasopressin

39. A metallo-enzyme that contains iron:

a. Lysyl oxidase
b. Superoxide dismutase
c. Cytochrome oxidase
d. Carbonic anhydrase
e. Tyrosinase

40. An activator of Carnitine acyl transferase:

a. Fatty acylCoA
b. Insulin
c. Malonyl CoA
d. Glucagon

41. The most common lethal autosomal recessive disorder of the Caucasians:

a. MCADdeficiency
b. Cystic fibrosis
c. Crigler-Najjar syndrome
d. Gilbert syndrome
e. Reye syndrome

42. Glycine can be obtained from all EXCEPT:

a. choline
b. arginine
c. serine
d. threonine

43. The activator of Pyruvate carboxylase is:

a. insulin
b. glucose
c. AMP
d. Acetyl CoA

44. Regarding degeneracy of the genetic code, Glycine has

a. 6 codons
b. 4 codons
c. 2 codons
d. 3 codons

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45. An example of Oxidoreductase:

a. Succinate dehydrogenase
b. Hexokinase
c. Glutamine synthetase
d. Acetyl CoA carboxylase

46. Trypsin is an example of

a. Transferases
b. Hydrolases
c. Lyases
d. Hydratases
e. Isomerases

47. Catecholamines are secreted from ____ of adrenal gland.

a. zona fasciculata
b. medulla
c. zona reticularis
d. zona glomerulosa

48. Normal Iron binding capacity:

a. 100-150 g/dl
b. 250-400 g/dl
c. 70-150 g/dl
d. 50-100 g/dl

49. 18-carbon steroids:

a. have androgenic activity

b. have estrogenic activity
c. include aldosterone
d. aromatization occurs in the testes

50. Phosphofructokinase 1

a. inhibited by ATP
b. inhibited by fructose-2,6-bisphosphate
c. decreased in fed state
d. inhibited by AMP

51. The net generation of ATP in citric acid cycle:

a. 12 ATP
b. 2 ATP
c. 32 ATP
d. 20 ATP
e. 38 ATP

52. A thiamine pyrophosphate dependent enzyme:

a. Aldehyde dehydrogenase
b. Kynureninase
c. Transketolase
d. ALA synthase
e. Xanthine oxidase

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53. HACEK group infection includes all of the following EXCEPT:

a. Actinomycetes naeslundii
b. Actinobacillus actinomycetemcomitans
c. Hemophilus aphrophilus
d. Cardiobacterium hominis
e. Kingella kingae

54. An example of Pentose:

a. Erythrose
b. Dihydroxyacetone
c. Glyceraldehyde
d. Ribulose
e. Mannose

55. A Pyridoxal phosphate (PLP) dependent enzyme:

a. Aldehyde dehydrogenase
b. Transketolase
c. Pyruvate Carboxylase
d. Kynureninase
e. Pyruvate dehydrogenase

56. The most common environmental poison in India is:

a. Mercury
b. Lead
c. Arsenic
d. Aluminium

57. A vitamin that acts as a coenzyme for carboxylation reactions

a. Biotin
b. Thiamin
c. Riboflavin
d. Pyridoxine
e. Niacin

58. Which of the following Trinucleotide sequence repeats are associated with Huntingon's chorea?

a. CAG repeats
b. CGG repeats
c. CTG repeats
d. CUG repeats

59. All of the following are conjugated proteins EXCEPT:

a. Rhodopsin
b. Histones
c. Ferritin
d. Hemoglobin
e. Ceruloplasmin

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60. An example of Triose:

a. Erythrose
b. Arabinose
c. Glyceraldehyde
d. Ribulose
e. Mannose

61. Reduction of Galactose results in:

a. Mannitol
b. Ribitol
c. Sorbitol
d. Dulcitol
e. Inositol

62. Activation of fatty acids to fatty acyl CoA is catalyzed by the enzyme:

a. Thiokinase
b. Glycerol kinase
c. Phosphofructokinase
d. Phosphorylase kinase
e. Pyruvate kinase

63. An enzyme found in the inner membrane, inner surface of mitochondria:

a. Glycerol-3-phosphate dehydrogenase
b. Adenylate kinase
c. Acyl CoA synthetase
d. Succinate dehydrogenase
e. Mono amino oxidase

64. Chymotrypsin has _____ polypetide chains and _____ number of amino acids.

a. 3z471
b. 2z800
c. 3z241
d. 4z574
e. 1z165

65. All of the following are Stop Codons EXCEPT:

a. UGA
b. UAA
c. AUG
d. UAG

66. Which of the following Shigella species ferments lactose?

a. Shigella flexneri
b. Shigella boydii
c. Shigella dysenteriae
d. Shigella sonnei

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67. Which among the following is NOT a pancreatic enzyme?

a. DNase I
b. Amylase
c. Chymotrypsin
d. Trypsin
e. Pancreozymin

68. INCORRECT about Erythropoietin (EPO):

a. functions as a cytokine
b. is a peptide hormone
c. is a glycoprotein hormone
d. involved in wound healing process
e. secreted by renal peritubular capillary endothelial cells

69. Which of the following glycosamino glycans (GAG) does NOT contain any uronic acid?

a. Hyaluronic Acid
b. Chondroitin Sulphate
c. Keratan Sulphate
d. Dermatan Sulphate
e. Heparin

70. The iron content of muscle myoglobin is:

a. 20 mg
b. 2600 mg
c. 600 mg
d. 200 mg

71. Virulence associated factors of Y. pestis include all EXCEPT:

a. hemolysin
b. pesticin 1
c. coagulase
d. fibrinolysin

72. Which of the following is/are NOT Pseudomonads?

a. Burkholderia pseudomallei
b. Ralstonia pickettii
c. Pseudomonas aeruginosa
d. Eikenella corrodens
e. Stenotrophomonas maltophilia

73. All of the following are mucopolysaccharides EXCEPT:

a. hyaluronic acid
b. heparin
c. amylopectin
d. chondroitin sulfate

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74. Among the HACEK group of bacterial species, which one is least/rarely associated with endocarditis?

a. Kingella kingae
b. Actinobacillus actinomycetemcomitans
c. Hemophilus aphrophilus
d. Cardiobacterium hominis
e. Eikenella corrodens

75. Cholecystokinin (CCK):

a. has 36 amino acids

b. glycoprotein hormone
c. it increases appetite
d. secreted by C cells of duodenum
e. secreted by gallbladder

76. All of the following are FAD-linked dehydrogenase EXCEPT:

a. Succinate dehydrogenase
b. Malate dehydrogenase
c. Fatty acyl CoA dehydrogenase
d. Glycerolphosphate dehydrogenase

77. An enzyme which is activated by magnesium:

a. 5'-de-iodinase
b. alkaline phosphatase
c. glutathione peroxidase
d. xanthine oxidase
e. pyruvate dehydrogenase

78. All of the following factors reduces iron absorption EXCEPT:

a. ascorbic acid
b. oxalic acid
c. milk
d. copper
e. phosphates

79. In eukaryotes, the process of transcription is carried out by the enzyme:

a. Reverse transcriptase
b. DNA polymerase
c. Aminoacyl-tRNA Synthetase
d. RNA polymerase

80. Which of the following is a 24-carbon compound?

a. testosterone
b. pregnenolone
c. cholesterol
d. bile acids
e. cortisol

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81. Y. pestis is highly sensitive to all of the following drugs EXCEPT:

a. penicillin
b. tetracyclines
c. streptomycin
d. chloramphenicol

82. Which of the following has the highest Calcium content among the given?

a. Soyabean
b. Drumstick leaves
c. Watermelon
d. Radish
e. Beetroot

83. Polycistronic mRNA:

a. contains information for amino acid sequences of different polypeptides

b. contains information for amino acid sequences of a single polypeptide
c. nonprotein-coding segment of DNA
d. Eukaryotic mRNA is always polycistronic
e. Prokaryotic mRNA molecules are always monocistronic

84. Oxalic acid can be produced from oxidation of which of the following compounds?

a. Methanol
b. Ethylene glycol
c. Para-nitrophenol
d. Aspartate
e. Oxaloacetate

85. All of the following are conjugating agents EXCEPT:

a. Glucuronic acid
b. Sulfate
c. Bilirubin
d. Acetic acid
e. Cysteine

86. Normal serum value of alkaline phosphatase (ALP):

a. 15-100 U/L
b. 10-80 U/L
c. 13-35 U/L
d. 10-30 U/L
e. 40-125 U/L

87. Fluoride inhibits enzyme enolase by removing which ions?

a. molybdenum
b. manganese
c. magnesium
d. selenium
e. zinc

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88. A Selenium containing enzyme:

a. methylcoenzyme reductase
b. superoxide dismutase
c. urease
d. tyrosinase
e. glutathione peroxidase

89. Which of the following is an intermediate of uronic acid pathway?

a. Xylulose
b. Ribulose
c. Ribose
d. O-glucuronide
e. Hexuronic acid

90. All of the following are trace elements EXCEPT:

a. magnesium
b. zinc
c. manganese
d. copper
e. iron

91. Which of the following is a NADP+ linked dehydrogenase?

a. Glyceraldehyde-3-phosphate dehydrogenase
b. glucose-6-phosphate dehydrogenase
c. Glycerolphosphate dehydrogenase
d. Pyruvate dehydrogenase
e. Succinate dehydrogenase

92. Enzyme phosphorylase kinase a is inactivated by:

a. glycogen synthase a
b. protein phosphatase-1
c. phosphorylase kinase b
d. cAMP-dependent protein kinase
e. inhibitor-1

93. Normal blood level of Chloride is:

a. 3.5-5 mEq/L
b. 1.8-2.2 mg/dl
c. 3-4 mg/dl
d. 136-145 mEq/L
e. 96-106 mEq/L

94. Which of the following is a monosaccharide?

a. Maltose
b. Xylose
c. Lactose
d. Sucrose
e. Amylose

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95. The activation of acetoacetate to acetoacetyl CoA is catalyzed by the enzyme

a. 7-alpha-hydroxylase
b. HMG CoA reductase
c. Thiophorase
d. Acetyl CoA synthase

96. The most common monosaccharides in nature:

a. D-fructose
b. D-ribose
c. 2-deoxy-D-ribose
d. D-galactose
e. L-arabinose

97. Which of the following statements is INCORRECT about Uronic acid pathway?

a. also known as alternative oxidative pathway for glucose

b. catalyzes the conversion of glucose to glucuronic acid
c. L-Gulonolactone is an intermediate
d. lead to the generation of ATP

98. Who first put forward the theory of enzyme catalysis?

a. Berzelius
b. Willy Kunhe
c. Edward Buchner
d. Ostwald
e. James Sumner

99. All of the following are involved in the biosynthesis of acetoacetate EXCEPT:

a. Acetone
b. Acetyl CoA
c. Acetoacetyl CoA
d. Hydroxymethyl glutaryl CoA

100. Which of the following has the highest iron content in mg/100 gm?

a. Bengal gram
b. Radish
c. Gram leaves
d. Amaranth
e. Soyabean

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 BIOCHEMISTRY
Answer Keys

1 c
2 b
3 b
4 a
5 c
6 a
7 b
8 d
9 c
10 c
11 a
12 c
13 c
14 b
15 c
16 a
17 a
18 d
19 c
20 b
21 c
22 b
23 d
24 d
25 a
26 a
27 a
28 a
29 c
30 a
31 b
32 b
33 d
34 b
35 b
36 a
37 b
38 c
39 c
40 d
41 b
42 b
43 d
44 b
45 a
46 b
47 b
48 b
49 b
50 a
51 d
52 c
53 a
54 d
55 d
56 b
57 a
58 a
59 b
60 c
61 d
62 a
63 d
64 c
65 c
66 d
67 e
68 b
69 c
70 d
Page 1
71 a
72 d
73 c
74 a
75 d
76 b
77 b
78 a
79 d
80 d
81 a
82 b
83 a
84 b
85 c
86 e
87 c
88 e
89 a
90 a
91 b
92 b
93 e
94 b
95 c
96 a
97 d
98 a
99 a
100 c

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NOTES