Distinguish between unique and highly repetitive sequences in nuclear
DNA
Unique single copy sequence Highly repetitive sequence
Occur once in genome Occur many times
Long base sequences Short sequences
Genes Not Genes
Translated Not translated
Small differences between individuals Can vary greatly
Exons Introns
Smaller proportion of genome Higher proportion of genome
Outline transcription in prokaryotes
- Transcription is the copying of a strand of DNA into RNA
- RNA polymerase binds to promoter region of DNA
- Anti-sense strand as template
- RNA polymerase unwinds DNA
- RNA nucleotides pair with complementary bases on DNA
- Adenine to Uracil, Guanine with Cytosine
- Strand grows 5’ to 3’
- RNA nucleotides joined with covalent bonds
- ENA polymerase separates from DNA when reaches terminator
- No introns, no post-transcriptional modification