Outline the structure of ribosome
- Made of protein and rRNA
- Large subunit and small subunit
- Three tRNA binding sites
- APE
- mRNA binding site
- 70s in prokaryotes, 80s in eukaryotes
- Can be free or bound to rER
Outline the structure of tRNA
- tRNA composed of one chain of nucleotides
- tRNA has a site attaching an amino acid at 3’ terminal
- tRNA has an anticodon
- Anticodon of three bases which are not base paired
- tRNA has double stranded sections formed by base pairing
- Double stranded sections can be helical
- tRNA has loops
- tRNA has a distinctive three dimensional clover leaf shape
Outline the process of crossing over
- Happens during prophase I of meiosis
- Homologous chromosomes exchange DNA
- Recombinants created
Outline the formation of recombinants during meiosis
- Recombination occurs in prophase I of meiosis
- Homologous chromosomes come together in pairs
- Chiasmata form between non-sister chromatids
- Crossing over takes place
Outline the random orientation in metaphase I leading to genetic variation
- When the chromosomes separate in meiosis to form haploid cells, there is random orientation of
homologous chromosomes to either each side of cell in metaphase I
- This is independent assortment of genes on different chromosome types
- Homologous chromosomes separate to opposite poles when attaching to spindle fibres
- There is 2n combinations of chromosomes, where n is the number of chromosomes pairs,
producing large genetic variation in haploid gametes
- There are effectively infinite number of combinations if effect of crossing over are included.