33 Hour Wholemount
•A: Optic Vesicle
•B: Foregut
•C: Heart
•D: Vitelline vein
•E: Opening to foregut
•F: Closed neural tube
•G: Somite
•H: Open neural tube
•I: Remnants of primitive streak
(arrowhead at anterior
neuropore)
33 Hour Wholemount: Rostral End
•A: Prosenchephalon
•B: Optic Vesicle
•C: Lens Placode
•D: Mesencephalon
•E: Rhombencephalon
•F: Heart
•G: Vitelline vein
•H: Forming foregut
•I: Neural tube
33 Hour Section 17
•A: Ectoderm
•B: Mesoderm
•C: Primitive Groove
•D: Newly formed Mesoderm
•E: Endoderm
33 Hour Section 14
•A: Ectoderm
•B: Lateral Plate Mesoderm
•C: Unsegmented Paraxial
Mesoderm
•D: Open neural tube
•E: Notochord
•F: Endoderm
•G: Neural Groove
33 Hour Section 12
•A: Ectoderm
•B: Lateral Plate Mesoderm
•C: Intermediate Mesoderm
•D: Somite
•E: Neural Tube
•F: Notochord
•G: Endoderm
33 Hour Section 11
•A: Ectoderm
•B: Somatic mesoderm
•C: Intermediate mesoderm
•D: Paraxial mesoderm
•E: Neural Tube
•F: Endoderm
•G: Splanchnic Mesoderm
•H: Splanchnopleure folding to
form foregut
•I: Notochord
•J Dorsal Aorta
•K: Somatic‐Splanchnic Junction
(letter K lies in intraembryonic
coelom)
33 Hour Section 10
•A: Ectoderm
•B: Somatic mesoderm
•C: Intermediate mesoderm
•D: Paraxial mesoderm
•E: Neural Tube
•F: Otic Placode
•G: Endoderm
•H: Splanchnic mesoderm
•I: Splanchnopleure folding to
form foregut
•J: Dorsal Aorta
•K: Notochord
•L: Somatic‐Splanchnic junction
(letter L lies in intraembryonic
coelom)
M: Vitelline vein
N: Primordium of gut muscularis
O: Future endodermal lining of
foregut
33 Hour Section 9
•A: Ectoderm
•B: Mesoderm
•C: Otic Placode
•D: Neural tube
•E: Dorsal Aorta
•F: Foregut
•G: Body folding (Somatopleure)
•H: Dorsal Mesocardium
•I: Epimyocardium of heart
•J: Endocardium of heart
•K: Endoderm
•L: Pericardial coelom
33 Hour Section 8
•A: Ectoderm
•B: Mesoderm
•C: Neural tube
•D: Anterior Cardinal vein
•E: Notochord
•F: Dorsal Aorta
•G: Foregut
•H: Body fold
•I: Somatic‐Splanchnic junction
•J: Splanchnic mesoderm
•K: Endoderm
•L: Heart
33 Hour Section 7
•A: Ectoderm
•B: Mesoderm
•C: Neural tube
•D: Notochord
•E: Dorsal Aorta
•F: Foregut
•G: Body fold
•H: Heart/Truncus Arteriosus
•I: Endoderm
•J: Somatic‐Splanchnic junction
33 Hour Section 5
•A: Ectoderm
•B: Mesoderm
•C: Neural tube
•D: Notochord
•E: Dorsal Aorta
•F: Foregut
•G: Ventral Aorta
•H: Oropharyngeal membrane
33 Hour Section 4
•A: Ectoderm
•B: Head mesenchyme
•C: Neural tube (Prosencephalon)
•D: Infundibulum
•E: 1st Aortic Arch
•F: Tangential section of foregut
33 Hour Section 3
•A: Ectoderm
•B: Head mesenchyme
•C: Prosencephalon
•D: Optic Vesicle
•E: Lens Placode
48 Hour Wholemount
•A: Myelencephalon
•B: Metencephalon
•C: Mesencephalon
•D: Diencephalon
•E: Optic Cup
•F: Telencephalon
•G: Heart
•H: Cephalic (Mesencephalic) Flexure
•I: Opening to foregut
•J: Closed neural tube
•K: Somite
•L: Open neural tube
•M: Vitelline artery
48 Hour Wholemount: Rostral End
•A: Somite
•B: 3rd Aortic arch
•C: Rood of myelencephalon
•D: Otocyst (Otic Vesicle)
•E: 2nd Aortic arch
•F: Metencephalon
•G: Mesencephalon
•H: Notochord
•I: Stomodeum
•J: Diencephalon
•K: Optic Cup
•L: Lens Vesicle
•M: Telencephalon
•N: Location of Olfactory placode
•O: Heart
•P: Opening to foregut
48 Hour Section 20
•A: Ectoderm
•B: Somatic mesoderm
•C: Intermediate mesoderm
•D: Dermomyotome
•E: Somitocoele
•F: Sclerotome
•G: Somatic‐Splanchnic junction
•H: Neural tube
•I: Notochord
•J: Endoderm
•K: Vitelline artery
•L: Splanchnic Mesoderm
•M: Extraembryonic coelom
48 Hour Section 19
•A: Edge of body fold
•B: Ectoderm
•C: Somatic Mesoderm
•D: Posterior Cardinal vein
(Intermediate Mesoderm)
•E: Dermomyotome
•F: Sclerotome
•G: Neural tube
•H: Notochord
•I: Fused Dorsal Aortae
•J: Mesonephric Tubule/Duct
•K: Splanchnic mesoderm
•L: Endoderm
48 Hour Section 18
•A: Forming foregut
•B: Endoderm
•C: Splanchnic mesoderm
•D: Ectoderm
•E: Somatic mesoderm
•F: Intermediate mesoderm
•G: Sclerotome
•H: Dermomyotome
•I: Neural tube
•J: Notochord
•K: Fused Dorsal Aortae
•L: Intraembryonic Coelom
•M: Posterior Cardinal vein
•N: Ventral edge of body fold
•O: Vitelline vein
48 Hour Section 16
•A: Heart
•B: Liver diverticulum
•C: Vitelline vein
•D: Liver diverticulum
•E: Foregut
•F: Splanchnic mesoderm of
foregut
•G: Fused Dorsal Aortae
•H: Intermediate mesoderm
•I: Sclerotome
•J: Dermomyotome
•K: Neural tube
•L: Notochord
•M: Posterior Cardinal vein
•N: Intraembryonic coelom
•O: Ventral edge of body fold
48 Hour Section 13
•A: Heart
•B: Sinus Venosus
•C: Intraembryonic coelom
•D: Splanchnic mesoderm of
foregut
•E: Endoderm of foregut
•F: Fused Dorsal Aortae
•G: Dermomyotome
•H: Sclerotome
•I: Neural tube
•J: Notochord
•K: Common Cardinal vein
entering Sinus Venosus
•L: Ventral edge of body fold
48 Hour Section 9
•A: Head mesenchyme
•B: Wall of Diencephalon
•C: Diencephalic wall continuous
with Optic Cup
•D: Diencephalon Infundibulum
•E: 1st Pharyngeal arch
•F: 1st Aortic arch
•G: Pharynx
•H: Dorsal Aorta
•I: Notochord
•J: Anterior Cardinal vein
•K: Thin roof plate of
Myelencephalon
•L: Myelencephalon ectoderm
•M: Otocyst (Otic Vesicle)
•N: 1st pharyngeal groove/
membrane
•O: Head ectoderm
•P: Lens Vesicle
•Q: Inner layer of Optic Cup
•R: Outer layer of Optic Cup
48 Hour Section 8
•A: Head mesenchyme
•B: Outer layer of Optic Cup
•C: Inner layer of Optic Cup
•D: Lens Vesicle continuous w/
surface ectoderm
•E: Rathke’s Pouch
•F: 1st Aortic arch
•G: 1st Pharyngeal groove/
membrane
•H: Dorsal Aorta
•I: Otocyst (Otic Vesicle)
•J: Ectoderm
•K: Thin roof plate of
Myelencephalon
•L: Otocyst continuous w/
surface ectoderm
•M: Anterior Cardinal vein
•N: Notochord
•O: Pharynx
•P: 1st Aortic Arch
•Q: Diencephalon Infundibulum
48 Hour Section 7
•A: Head mesenchyme
•B: Diencephalon
•C: Anterior Cardinal vein
•D: Dorsal Aorta continuous w/
1st Aortic arch
•E: Myelencephalon
•F: Anterior Cardinal vein
•G: Notochord
48 Hour Section 5
•A: Head mesenchyme
•B: Thin roof of Myelencephalon
•C: Metencephalon
•D: Isthmus
•E: Mesencephalon
