specifications bulletin 5595
C1000™ Thermal Cycler
The C1000 thermal cycler provides superb thermal
performance for fast, reliable results. This fully modular
platform supports interchangeable reaction modules —
including two optical modules for real-time PCR — that
swap in seconds, without requiring tools. Equipped with a
state-of-the-art interface, the C1000 cycler offers multiple
programming methods, including graphical, text based,
and automatic (using the protocol autowriter).
n Interchangeable reaction modules, including five-color
CFX96™ optical, four-color CFX384™ optical, gradient-
enabled dual 48/48 fast, gradient-enabled 96-well fast,
and gradient-enabled 384-well reaction modules
n USB ports that support peripherals, such as storage
device and mouse
n O
ptional PC control and networking capability for up to
32 systems enable the ultimate in high throughput
Specifications
Thermal Cycler (cont.)
Input power 400 W, maximum Programming options Step-based graphical, text based, automatic
Frequency 50–60 Hz, single phase Security features Password-protected folders,
Display 12 x 9 cm, high resolution, color optional log-in, and secured mode
Ports 5 USB A, 1 USB B for highly regulated environments
Fuses Two 6.3 A, 250 V, 5 x 20 mm Reporting Exportable run logs, system error logs
Memory >1,000 typical programs onboard Onboard software Windows CE 6.0
Unlimited with USB flash drive PC compatibility Windows XP or higher
Dimensions (W x D x H) 13 x 18 x 8" USB peripheral compatibility Mouse, USB flash drive, bar code reader
Weight 23 lb Real-time upgrade 6-channel, 5-color CFX96 or 5-channel,
4-color CFX384 optical reaction modules
Temperature control modes Calculated and block Instant incubation Yes
PCR license Yes
Reaction Modules 96-Well Fast Dual 48/48 Fast 384-Well
Sample capacity 96 x 0.2 ml tubes or 2 x 48 x 0.2 ml tubes 1 x 384-well PCR plate
1 x 96-well PCR plate
Maximum ramp rate 5°C/sec 4°C/sec 2.5°C/sec
Average ramp rate 3.3°C/sec 3°C/sec 2°C/sec
Temperature range 0–100°C 0–100°C 0–100°C
Temperature accuracy ±0.2°C of programmed target at 90°C ±0.2°C of programmed target at 90°C ±0.2°C of programmed target at 90°C
Temperature uniformity ±0.4°C well-to-well within 10 sec of ±0.4°C well-to-well within 10 sec of ±0.4°C well-to-well within 10 sec of
arrival at 90°C arrival at 90°C arrival at 90°C
Gradient capability Yes Yes Yes
Gradient
Gradient accuracy ±0.2°C of programmed temperature at end rows
Row uniformity ±0.4°C well-to-well (within row) within 10 sec of arrival at target temperature
Gradient range 30–100°C
Temperature differential range 1–24°C
Ordering Information
Catalog # Description
184-1000 1000 Thermal Cycler Chassis, includes USB flash drive,
C
C1000 Manager™ software CD, power cord, instruction
manual; does not include reaction module
185-1048R C1000 Thermal Cycler With Dual 48/48 Fast Reaction
Module, includes thermal cycler chassis, dual 48/48
fast reaction module, USB flash drive, C1000 Manager
software CD, power cord, reagent and consumable
samples, instruction manual
185-1096R C1000 Thermal Cycler With 96-Well Fast Reaction
Module, includes thermal cycler chassis, 96-well fast
reaction module, USB flash drive, C1000 Manager
Quick and easy protocol programming. The protocol autowriter in the software CD, power cord, reagent and consumable
cycler’s onboard software can automatically suggest a fast temperature samples, instruction manual
protocol based on input parameters. Suggested protocol is based on 185-1384R 1000 Thermal Cycler With 384-Well Reaction Module,
C
standard PCR guidelines, with hot-start, initial denaturation, annealing, includes thermal cycler chassis, 384-well reaction module,
and extension steps. Further reductions of run times are achieved USB flash drive, C1000 Manager software CD, power cord,
by minimizing the number of steps and cycles, incubation times, and reagent and consumable samples, instruction manual
temperature differentials.
184-0048 ual 48/48 Fast Reaction Module, 2 independent
D
48-well blocks, fits C1000 and S1000 thermal cyclers
90 184-0096 6-Well Fast Reaction Module, fits C1000
9
and S1000 thermal cyclers
85
184-0384 384-Well Reaction Module, fits C1000 and
80 S1000 thermal cyclers
Temperature, °C
184-4000 C1000 Manager Software, version 1.0, includes
75
installation CD, instructions
70 184-5096 CFX96 Optical Reaction Module, includes CFX Manager™
software, license for qbase PLUS software, communication
65 cable, reagent and consumable samples, instruction manual
184-5384 CFX384 Optical Reaction Module, includes CFX Manager
60
software, license for qbase PLUS software, communication
55 cable, reagent and consumable samples, instruction manual
0 20 40 60 80 100 120 140 160 180 200 170-8870 iTaq™ DNA Polymerase, 5 U/µl
Time, sec
172-5301 iProof™ High-Fidelity DNA Polymerase, 2 U/µl, 100 U
Rapid arrival at target temperature and superior uniformity. Graph
shows temperature measured by probes in 15 wells across the sample Windows is a trademark of Microsoft Corporation.
block of a C1000 thermal cycler. Traces are nearly indistinguishable due Purchase of this instrument conveys a limited non-transferable immunity
to high uniformity. Note the consistent ramp rate throughout heating and from suit for the purchaser’s own internal research and development and
cooling. 1000-series thermal cyclers exhibit high average ramp rates, rapid for use in human in vitro diagnostics and all other applied fields under one
settling time, and tight thermal uniformity throughout the ramp, resulting in or more of U.S. Patents 5,656,493, 5,333,675, 5,475,610 (claims 1, 44, 158,
rapid arrival at target temperature and enabling faster protocol run times. 160–163 and 167 only), and 6,703,236 (claims 1–7 only), or corresponding
claims in their non-U.S. counterparts, owned by Applera Corporation. No
right is conveyed expressly, by implication or by estoppel under any other
patent claim, such as claims to apparatus, reagents, kits, or methods such
as 5’ nuclease methods. Further information on purchasing licenses may be
obtained by contacting the Director of Licensing, Applied Biosystems, 850
Lincoln Centre Drive, Foster City, California 94404, USA.
Bio-Rad’s real-time thermal cyclers are licensed real-time thermal cyclers
under Applera’s United States Patent 6,814,934 B1 for use in research,
human in vitro diagnostics, and all other fields except veterinary diagnostics.
This product is covered by one or more of the following U.S. patents
or their foreign counterparts owned by Eppendorf AG: U.S. Patent Nos.
6,767,512 and 7,074,367.
Practice of the patented 5' Nuclease Process requires a license from
Applied Biosystems. The purchase of these products includes an immunity
Multi-instrument control. The C1000 cycler can control multiple thermal from suit under patents specified in the product insert to use only the amount
cyclers using a single interface. purchased for the purchaser’s own internal research when used with the
separate purchase of Licensed Probe. No other patent rights are conveyed
expressly, by implication, or by estoppel. Further information on purchasing
licenses may be obtained from the Director of Licensing, Applied Biosystems,
850 Lincoln Centre Drive, Foster City, California 94404, USA.
Bio-Rad
Laboratories, Inc.
Life Science Web site www.bio-rad.com USA 800 4BIORAD Australia 61 02 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 21 3237 9400
Canada 905 364 3435 China 86 21 6426 0808 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00 France 01 47 95 69 65
Group Germany 089 318 84 0 Greece 30 210 777 4396 Hong Kong 852 2789 3300 Hungary 36 1 455 8800 India 91 124 4029300 Israel 03 963 6050
Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 0318 540666 New Zealand 0508 805 500
Norway 23 38 41 30 Poland 48 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 27 861 246 723
Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 061 717 95 55 Taiwan 886 2 2578 7189 United Kingdom 020 8328 2000
Bulletin 5595 Rev B US/EG 09-1000 1109 Sig 0308
