The Effect of Temperature on
Biological Membranes
Lab Report
Namarik Al-Enizy
Tuesday December 1, 2009
IB Biology Year 1
Mrs. Daena
QUALITATIVE OBSERVATIONS:
TEST TUBE RATE OF PIGMENTATION OBSERVATIONS
NUMBER (FROM A SCALE OF 1 TO 6)
(1 BEING LIGHTEST)
1 3 Clear on the surface, with faint pink
residue on the bottom.
2 2 Light pink on the top; pigmentation
gradually increases down the test tube.
3 1 A light pink color on the surface, with
darker colored residue on the bottom of
the test tube.
4 4 A dark pink at the top of the test tube,
with a light red pigment at the bottom.
5 5 Light red on the surface; color increases
deeper into the test tube, making the
pigmentation very concentrated at the
bottom.
6 6 The color is very dark red throughout;
almost blood red; the bottom of the test
tube is so dark that it is almost black-
colored.
Namarik Al-Enizy
Tuesday December 1, 2009
IB Biology Year 1
Mrs. Daena
RED ABSORBANCE {Trial 1}
% TRANSMITTANCE (+-
SUBSTANCE TEMPERATURE (degrees Celsius) 0.5%)
Pure Distilled
Water none 0.000
Test tube 1 30 0.041
Test Tube 2 40 0.054
Test Tube 3 50 0.006
Test Tube 4 60 0.036
Test Tube 5 70 0.094
Test Tube 6 80 0.240
*NOTE: the wavelength of the red pigment Average:
that was being monitored was 660 nm. 0.067
Formula for calculation of
average:
the sum of all the values÷
the number of values (in this case, 7)
Namarik Al-Enizy
Tuesday December 1, 2009
IB Biology Year 1
Mrs. Daena
RED ABSORBANCE (BAR GRAPH)
{Trial 1}
0.200
% TRANSMITTANCE
0.100
RED ABSORBANCE
0.000
er e1 e2 e3 e4 e5 e6
at b b b ub ub ub
W tu Tu Tu T T T
l ed est st st st st st
ti l T T e T e Te Te Te
D is
re
Pu
SUBSTANCE (TEST TUBE NUMBER)
RED ABSORBANCE {Trial 2}
TEMPERATURE (degrees % Transmittance (+-
SUBSTANCE Celsius) 0.5%)
Pure Distilled Water none 0.000
Test Tube 1 30 0.011
Test Tube 2 40 0.008
Test Tube 3 50 0.019
Test Tube 4 60 0.015
Test Tube 5 70 0.036
Test Tube 6 80 0.108
*Note: the wavelength of the
red pigment Average:
that was being monitored was 660 nm. 0.028
Formula for calculation of
average:
the sum of all the values÷
the number of values (in this
case, 7)
Namarik Al-Enizy
Tuesday December 1, 2009
IB Biology Year 1
Mrs. Daena
RED ABSORBANCE (BAR GRAPH)
{Trial 2}
0.150
0.100
% TRANSMITTANCE
0.050
0.000
Pure Distilled Water
Test Tube 1
Test Tube 2
Test Tube 3
Test Tube 4
Test Tube 5
Test Tube 6
SUBSTANCE (TEST TUBE NUMBER)
RED ABSORBANCE (PIE CHART)
Pure Distilled Water
Test tube 1
Test Tube 2
Test Tube 3
Test Tube 4
Test Tube 5
Test Tube 6
Pure Distilled
Water 0.000
Test Tube 1 0.011
Test Tube 2 0.008
Test Tube 3 0.019
Test Tube 4 0.015
Test Tube 5 0.036
Namarik Al-Enizy
Tuesday December 1, 2009
IB Biology Year 1
Mrs. Daena
Test Tube 6 0.108
TOTAL AVERAGE RED ABSORBANCE
TRIAL 1 0.067
TRIAL2 0.028
TOTAL AVERAGE RED ABSORBANCE
FOR BOTH TRIALS
% TRANSMITTANCE
0.07
0.06
0.05
0.04
0.03
0.02
0.01
0
TRIAL 1 TRIAL2
TRIAL NUMBER
AVERAGE RED ABSORBANCE FOR EACH TEST TUBE
% TRANSMITTANCE 1ST % Transmittance 2ND TRIAL
SUBSTANCE TRIAL(+-0.5%) (+-0.5%) AVERAGE
Pure Distilled
Water 0.000 0.000 0.000
Test tube 1 0.041 0.011 0.026
Test Tube 2 0.054 0.008 0.031
Test Tube 3 0.006 0.019 0.012
Test Tube 4 0.036 0.015 0.025
Test Tube 5 0.094 0.036 0.065
Test Tube 6 0.240 0.108 0.174
Namarik Al-Enizy
Tuesday December 1, 2009
IB Biology Year 1
Mrs. Daena
AVERAGE RED ABSORBANCE FOR EACH
INDIVIDUAL TEST TUBE
AVERAGE % TRANSMITTANCE
0.180
0.120
0.060
0.000
er 1 2 3 4 5 6
at ube ube ube ube ube ube
W tt T T T T T
d s st st st st st
le Te
til Te Te Te Te Te
D is
ure
P
SUBSTANCE/ TEST TUBE NUMBER
Namarik Al-Enizy
Tuesday December 1, 2009
IB Biology Year 1
Mrs. Daena
