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Chol

This document provides instructions for validating a cholesterol assay on an ARCHITECT C8000 instrument. It outlines the instrument settings, reaction definition, reagent and sample details, calibration information including standard levels and intervals, and validity check parameters for the CHOL reagent which uses a cholesterol oxidase/peroxidase enzymatic method. Results are reported in mg/dL with 1 decimal place.

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Enrique Duarte
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80 views1 page

Chol

This document provides instructions for validating a cholesterol assay on an ARCHITECT C8000 instrument. It outlines the instrument settings, reaction definition, reagent and sample details, calibration information including standard levels and intervals, and validity check parameters for the CHOL reagent which uses a cholesterol oxidase/peroxidase enzymatic method. Results are reported in mg/dL with 1 decimal place.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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CHOLESTEROL

Enzymatic-spectrophotometric
CHOLESTEROL OXIDASE/PEROXIDASE

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: ARCHITECT C8000


Reagent preparation
Reagent is ready to use.

Instrument settings

General Conventional Units


Assay CHOL Type Photometric Results Unit mg/dL
Number ... Version 1 Assay Defaults Low Linearity 0.3
High Linearity 1000
Reaction Definition
Reaction Mode End Up Read Times Gender and Age specific Ranges
Wavelength 500 / 0 Main 16 -- 18 GENDER AGE (Y) NORMAL
Last Required Read 18 Flex __ -- __ Male 0-130 0 - 239
Absorbance Range … / … Color Correction __ -- __ Female 0-130 0 - 239
Sample Blank Type None Blank Read Time __ -- __ Either 0-130 0 - 239

Reagent / Sample R1 R2 Reults Units mg/dL


Reagent CHOL Reagent Volume 300 Decimal Places 1 (Range 0-4)
Diluent Saline Water Volume __ __ Correlation Factor 1.0000
Diluent Disp. Mode Type 0 Dispense Mode Type 0 Type 0 Intercept 0.0000

Diluent Name Sample Water Dilution Factor


Standard 3 1:1.00

Validity Checks
Reaction definition None
Rate Linearity % ___
Calibration
Method Linear

Calibrators and Volumes


Calibrator Set Standard Calibrator Level Concentration Volume
Blank 0.0 3
Std. 1 (*) 3
Std. 2 -- --
Std. 3 -- --
Std. 4 -- --
Replicates 3 (Range 1-3) Std. 5 -- --

Intervals Validity Checks


Calibration Interval Full Interval 999 hours Blank Absorbance range: 0.0000 -- 0.0000
Calibration Type Adjust Type None Span Absorbance Range: 0.0000 -- 0.0000
Expected cal Factor : 0.00
Expected Cal factor tolerance: %0
(*) Enter the standard value
… Values to be entered by the user
Version 0707

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