Scribd
Upload
95 views1 page

Alanine Aminotransferase (Alt/Gpt) : Instrument

This document provides instrument settings and parameters for performing an ALT/GPT assay using a continuous spectrophotometric method on an AU 400/640 instrument. It details reagent preparation instructions, calibration information including a two-point master curve calibration, and specifies acceptable measurement ranges for samples, controls, and standards. Quality control parameters like correlation factor, linearity, and panic values are also listed.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
95 views1 page

Alanine Aminotransferase (Alt/Gpt) : Instrument

This document provides instrument settings and parameters for performing an ALT/GPT assay using a continuous spectrophotometric method on an AU 400/640 instrument. It details reagent preparation instructions, calibration information including a two-point master curve calibration, and specifies acceptable measurement ranges for samples, controls, and standards. Quality control parameters like correlation factor, linearity, and panic values are also listed.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 1

ALANINE AMINOTRANSFERASE (ALT/GPT)

Continuous-spectrophotometric
IFCC

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: AU 400 / 640


Reagent preparation
Working Reagent: Pour the contents of the Reagent B into the Reagent A bottle. Mix gently.
Stable for 2 months at 2-8ºC.

Instrument settings

Specific Test Parameters Min OD Max OD


Sample: Volume 10 µL Dilution 0 µL L 0.6 H 2.5
Reagents: R1 Volume 200 µL Dilution 0 µL Reagent OD limit:
Wavelength: Pri. 340 Sec. 660 First L 1.0 First H 2.5
Method: RATE Last L 1.0 Last H 2.5
Reaction slope: - Dynamic Range:
Measuring Point 1: First 7 Last 20 L 0 H 500
Measuring Point 2: First 0 Last 0 Correlation Factor:
Linearity: 15 % A 1 B 0
No Lag Time: NO On-board stability period:

L H
7. None selected
8. Out of range 0 41
L H
Panic Value Unit: U/L Decimal places: 0

Calibration Specific

Calibration Type: MB Formula: Y=AX+B Counts: 2 Process: CONC.

Cal. Nº OD CONC Factor/OD-L Factor/OD-H


Point 1: # * -9999 9999

MB Type Factor: 5555

* Enter the assigned standard value


# User defined

Vers. 0505

You might also like