BTB 309 Molecular Breeding of Horticultural
Crops and Forest Trees (2+1)
Topic: Mutational Breeding in Tuberose
Course Teacher: Submitted by:
Dr. N. Manikanda Boopathi P. Indhumathi
Course Associate: 2020012016
Dr. R. Poornima B. Tech. Biotechnology
UG III Year
INTRODUCTION:
Tuberose:
Scientific name: Poliantha tuberosa
Family : Amrylidaceae
Origin : Mexico
Chromosome number: 2n=30
Climate : Tropical climate
Optimum pH : 6.5 – 7.5
Propagation : Bulbs
Planting time : March to April
Spacing : 30 x 20 cm / 20 x 15 cm
https://vikaspedia.in
IMPORTANCE AND USES:
• Can grow in pots, borders, and beds
• artistic garlands, floral ornaments, gajras,and extraction of essential oil
• Provide fragrance to bouquets and boutonnieres
• Table decoration
• Aromatherapy
• Applied as a paste over the red pimples of infants
• Remedy for gonorrhea
https://vikaspedia.in
Types:
• Single
• Semi-double
• Double and
• Variegated
https://vikaspedia.in
MUTATIONAL BREEDING:
Lalit and Manish, 2020
Article 1:
Mutagenesis via exposure to gamma-rays:
Taken: Double type bulbs
Bulbs- grown and harvested
Bulbs - stock 4-6°C
Washed- water(24 hours)
Fungicide Benomyl (5%)
Gamma-ray via source of cobalt (Co60)
Planted
Measured traits
Navabi et al., 2016
Results and discussion:
Navabi et al., 2016
Results and discussion:
Navabi et al., 2016
Conclusion:
• Experimental results showed that at the dose treatment of 10 Gy, all plants
sprouted with a time delay.
• At doses of 50 and 100 Gy, 57% and 29% of plants were sprouted and grew,
respectively.
• At dosages of 200 and 400 Gy, none of the plants survived.
Navabi et al., 2016
Article 2:
Mutagenesis by EMS:
1. Bulbs of Prajwal Tuberose were dipped in five levels of EMS 0.25, 0.50, 0.75,
1.0% EMS solution for three dipping times for 5 min, 4 hours and 8 hours.
2. Sixteen plants of each treatment combination were planted in each replication
at spacing of 30cm x 30 cm in plots having area of 1.2 m x 1.2 m
3. Traits measured for statistical analysis to have meaningful interpretations
Yadav et al.,
2022
Results and discussion:
(1) Important flower traits for tuberose Plants
Yadav et al., 2022
Results and discussion:
Yadav et al., 2022
Results and discussion:
Yadav et al., 2022
Results and discussion:
Yadav et al., 2022
(2) Important Bulb traits
Yadav et al., 2022
Results and discussion:
Yadav et al., 2022
Conclusion:
• The treatment of bulbs with mutagenesis EMS 0.25% with 8 hours dipping
duration was more promising to increase in plant height, number of leaves per
clump, length of leaves, spike length, rachis length, spike weight, number of
opened florets, number of unopened florets and number of spikes per clump.
• The treatment of bulbs with 0.50% EMS with 4 hours dipping duration in EMS
solution shows an increase in number of bulbs per clump, diameter of bulb and
weight of bulbs.
Yadav et al., 2022
Overall Conclusion:
• Chemical mutagens > physical mutagens
• The concentration and time determines the effect of the mutation.
• Duration and doses - survival rate and vice versa.
