Single-molecule fluorescence trace extraction and analysis
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Updated
Nov 11, 2025 - Python
Single-molecule fluorescence trace extraction and analysis
File format agnostic low level, high performance API to read and process time-tagged-time resolved (TTTR) data for single-molecule and image spectroscopy (Sample data: https://gitlab.peulen.xyz/skf/tttr-data)
MATLAB package to analyze single-molecule FRET data
Volumetric Localization Microscopy with Deep Learning
This is a repo for a Python based application to open SMS .h5 files, and perform the initial analysis and exporting.
ASCAM is Advanced Single Channel Analysis for Mac (and others)
This code accompanies the following publication: '“Turbo-Charged” DNA Motors with Optimized Sequence Enable Single-Molecule Nucleic Acid Sensing' by Zhang & Piranej et al., Angewandte Chemie International Edition 2024
PhD-Level Course for Single-Molecule Sequencing Technologies
Lima - Demultiplex Barcoded PacBio Samples
A modular single-molecule analysis interface
Single Molecule Localization Microscopy, Tracking Simulations for Baljyot Parmar at the Weber Lab. Montreal, QC.
Burst analysis software for single and multi-spot single-molecule FRET (smFRET) data.
Single-moecule imaging and computation with RNA
Matlab software for simulation of two-dimensional single particle tracking (SPT) data sets.
Single-molecule force spectroscopy calibration
Maximum likelihood fits for low photon count data - For active develeopment visit gitlab.peulen.xyz
Detection of Molecules plugin for ImageJ, Utrecht University
Single Molecule Fluorescence Simulator Toolkit
Dwell time analysis of single-molecule force-spectroscopy data
A useful tool for working with Oxford Nanoimager microscope single-molecule localization data.
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