Izvorni kôd stranice CCL8

{{Infokutija gen}}
'''Hemokinski (C-C motivni) ligand 8''' (CCL8), znan i kao  '''monocitni hemoatraktantni protein 2''' (MCP2), je [[protein]] koji je kod [[ljudi]] [[kodon|kodiran]] [[gen]]om '''CCL8'''.<ref name="entrez">{{cite web | title = Entrez Gene: chemokine (C-C motif) ligand 8| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6355}}</ref><ref name="pmid9119400">{{cite journal | vauthors = Van Coillie E, Fiten P, Nomiyama H, Sakaki Y, Miura R, Yoshie O, Van Damme J, Opdenakker G | title = The human MCP-2 gene (SCYA8): cloning, sequence analysis, tissue expression, and assignment to the CC chemokine gene contig on chromosome 17q11.2 | journal = Genomics | volume = 40 | issue = 2 | pages = 323–31 |date=mart 1997 | pmid = 9119400 | doi = 10.1006/geno.1996.4594 }}</ref>

CCL8 je mali [[citokin]] koji pripada porodici CC [[hemokin]]a. CCL8 protein se proizvodi kao [[proteinski prekursor|prekursor]] koji sadrži 109 [[aminokiselina]], a koji se cijepa da bi se dobio zreli CCL8 koji sadrži 75 aminokiselina. Kod ljudi, [[gen]] za CCL8 ima tri [[egzon]]a i nalazi se unutar velikog skupa CC hemokinskih gena na [[hromosom 17|hromosomu 17]] q11.2.<ref name="pmid9119400"/><ref name="pmid1613466">{{cite journal | vauthors = Van Damme J, Proost P, Lenaerts JP, Opdenakker G | title = Structural and functional identification of two human, tumor-derived monocyte chemotactic proteins (MCP-2 and MCP-3) belonging to the chemokine family | journal = J. Exp. Med. | volume = 176 | issue = 1 | pages = 59–65 |date=juli 1992 | pmid = 1613466 | pmc = 2119277 | doi = 10.1084/jem.176.1.59}}</ref> MCP-2 je [[hemotaksija|hemotaksijski]]  i aktivira mnoge različite imunske ćelije, uključujući [[mastocit]]e, [[eozinofil]]e i [[bazofil]]e (koje su uključene u [[alergijske reakcije]]) i [[monocit]]e, [[T-ćelije]] i [[NK-ćelije]] koji su uključeni u [[upala|upalni]] odgovor.<ref name="pmid8558070">{{cite journal | vauthors = Proost P, Wuyts A, Van Damme J | title = Human monocyte chemotactic proteins-2 and -3: structural and functional comparison with MCP-1 | journal = J. Leukoc. Biol. | volume = 59 | issue = 1 | pages = 67–74 |date=januar 1996 | pmid = 8558070 | doi = 10.1002/jlb.59.1.67| s2cid = 30445255 }}</ref><ref name="Gong_1998">{{cite journal | vauthors = Gong W, Howard OM, Turpin JA, Grimm MC, Ueda H, Gray PW, Raport CJ, Oppenheim JJ, Wang JM | title = Monocyte chemotactic protein-2 activates CCR5 and blocks CD4/CCR5-mediated HIV-1 entry/replication | journal = J. Biol. Chem. | volume = 273 | issue = 8 | pages = 4289–92 |date=februar 1998 | pmid = 9468473 | doi = 10.1074/jbc.273.8.4289| doi-access = free }}</ref>  Svoje efekte CCL8 izaziva vezanjem na nekoliko različitih receptora na ćelijskoj površini, zvanih [[hemokinski receptor]]i. Ovi receptori uključuju [[CCR1]], [[CCR2B]], [[CCR3]] i [[CCR5]].<ref name="Gong_1998"/><ref>{{cite journal| pmid=29281969 | doi=10.1186/s12865-017-0237-5 | volume=18 | title=Functional expression of CCL8 and its interaction with chemokine receptor CCR3 | pmc=5745793 | year=2017 | journal=BMC Immunol | page=54 | vauthors=Ge B, Li J, Wei Z, Sun T, Song Y, Khan NU| issue=1 }}</ref>

CCL8 je CC-hemokin koji koristi više ćelijskih receptora za privlačenje i aktiviranje ljudskih leukocita. CCL8 je snažni inhibitor [[HIV]] 1, zahvaljujući visokom afinitetu vezanja za receptor CCR5, jedan od glavnih koreceptora za HIV1.<ref name="pmid11087354">{{PDB|1ESR}}; {{cite journal | vauthors = Blaszczyk J, Coillie EV, Proost P, Damme JV, Opdenakker G, Bujacz GD, Wang JM, Ji X | title = Complete crystal structure of monocyte chemotactic protein-2, a CC chemokine that interacts with multiple receptors | journal = Biochemistry | volume = 39 | issue = 46 | pages = 14075–81 | date=novembar 2000 | pmid = 11087354 | doi = 10.1021/bi0009340}}</ref> Pored toga, CCL8 –u pripisuje se rast [[metastaza]] u ćelijama [[rak dojke|raka dojke]]. Manipulacija ovom hemokinskom aktivnošću utiče na histologiju tumora, promovirajući korake metastatskih procesa.<ref>{{cite journal| pmc=5112152 | pmid=27181207 | doi=10.1038/onc.2016.161 | volume=35 | title=A CCL8 gradient drives breast cancer cell dissemination | year=2016 | journal=Oncogene | pages=6309–6318 | vauthors=Farmaki E, Chatzistamou I, Kaza V, Kiaris H| issue=49 }}</ref> CCL8 je također uključen u privlačenje makrofaga u porođajnu deciduu.<ref>{{cite journal| pmid=23451115 | doi=10.1371/journal.pone.0056946 | volume=8 | title=Identification of chemokines associated with the recruitment of decidual leukocytes in human labour: potential novel targets for preterm labour | pmc=3579936 | year=2013 | journal=PLOS ONE | page=e56946 | vauthors=Hamilton SA, Tower CL, Jones RL| issue=2 }}</ref>

==Aminokiselinska sekvenca==
Dužina [[polipeptid]]nog lanca je  99  [[aminokiselina]], a [[molekularna masa|molekulska težina]]   11.246 [[dalton (jedinica)|Da]].<ref name="UniProt">{{cite web|url=http://www.uniprot.org/uniprot/P80075#sequences |title=UniProt, P80075 |access-date=27. 6. 2021}}</ref> 
<table  style="font-family:monospace;">
<tr style="text-align:right;"><th>10</th><th></th><th>20</th><th></th><th>30</th><th></th><th>40</th><th></th><th>50</th></tr>
<tr style="background-color: #eee;"><td>MKVSAALLCL</td><td style="background-color: #f9f9f9;"></td><td>LLMAATFSPQ</td><td style="background-color: #f9f9f9;"></td><td>GLAQPDSVSI</td><td style="background-color: #f9f9f9;"></td><td>PITCCFNVIN</td><td style="background-color: #f9f9f9;"></td><td>RKIPIQRLES</td></tr>
<tr style="background-color: #eee;"><td>YTRITNIQCP</td><td style="background-color: #f9f9f9;"></td><td>KEAVIFKTKR</td><td style="background-color: #f9f9f9;"></td><td>GKEVCADPKE</td><td style="background-color: #f9f9f9;"></td><td>RWVRDSMKHL</td><td style="background-color: #f9f9f9;"></td><td>DQIFQNLKP</td></tr>
</table>
:Simboli
{{refbegin|4}}
'''C''':  [[Cistein]] <br>
'''D''': [[Asparaginska kiselina]] <br>
'''E''': [[Glutaminska kiselina]] <br>
'''F''':  [[Fenilalanin]] <br>
'''G''':  [[Glicin]] <br>
'''H''':  [[Histidin]] <br>
'''I''':  [[Izoleucin]] <br>
'''K''': [[Lizin]] <br>
'''L''': [[Leucin]] <br>
'''M''':  [[Metionin]] <br>
'''N''':  [[Asparagin]] <br>
'''P''':  [[Prolin]] <br>
'''Q''':  [[Glutamin]] <br>
'''R''':  [[Arginin]] <br>
'''S''':  [[Serin]] <br>
'''T''':  [[Treonin]] <br>
'''V''':  [[Valin]] <br>
'''W''':  [[Triptofan]] <br>
'''Y''':  [[Tirozin]]
{{refend}}

==Reference==
{{reflist}}

==Vanjski linkovi==
* {{UCSC gene info|CCL8}}

==Dopunska literatura==
{{refbegin | 2}}
*{{cite journal  |vauthors=Struyf S, Proost P, Vandercappellen J |title=Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects. |journal=Eur. J. Immunol. |volume=39 |issue= 3 |pages= 843–57 |year= 2009 |pmid= 19224633 |doi= 10.1002/eji.200838660 |display-authors=etal|doi-access=free }}
*{{cite journal  |vauthors=Rom S, Rom I, Passiatore G |title=CCL8/MCP-2 is a target for mir-146a in HIV-1-infected human microglial cells. |url=https://archive.org/details/sim_faseb-journal_2010-07_24_7/page/2292 |journal=FASEB J. |volume=24 |issue= 7 |pages= 2292–300 |year= 2010 |pmid= 20181935 |doi= 10.1096/fj.09-143503 |pmc=2887261|display-authors=etal}}
*{{cite journal  |vauthors=Menon R, Pearce B, Velez DR |title=Racial disparity in pathophysiologic pathways of preterm birth based on genetic variants. |journal=Reprod. Biol. Endocrinol. |volume=7 |pages= 62 |year= 2009 |pmid= 19527514 |doi= 10.1186/1477-7827-7-62 |pmc=2714850|display-authors=etal}}
*{{cite journal  |vauthors=Segat L, Brandão LA, Guimarães RL |title=Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment. |journal=Vaccine |volume=28 |issue= 10 |pages= 2201–6 |year= 2010 |pmid= 20056178 |doi= 10.1016/j.vaccine.2009.12.056 |display-authors=etal}}
*{{cite journal  |vauthors=Rajaraman P, Brenner AV, Butler MA |title=Common variation in genes related to innate immunity and risk of adult glioma. |journal=Cancer Epidemiol. Biomarkers Prev. |volume=18 |issue= 5 |pages= 1651–8 |year= 2009 |pmid= 19423540 |doi= 10.1158/1055-9965.EPI-08-1041 |pmc=2771723|display-authors=etal}}
*{{cite journal  |vauthors=Han S, Lan Q, Park AK |title=Polymorphisms in innate immunity genes and risk of childhood leukemia. |journal=Hum. Immunol. |volume=71 |issue= 7 |pages= 727–30 |year= 2010 |pmid= 20438785 |doi= 10.1016/j.humimm.2010.04.004 |pmc=2967770|display-authors=etal}}
*{{cite journal  |vauthors=Velez DR, Fortunato SJ, Thorsen P |title=Preterm birth in Caucasians is associated with coagulation and inflammation pathway gene variants. |journal=PLOS ONE |volume=3 |issue= 9 |pages= e3283 |year= 2008 |pmid= 18818748 |doi= 10.1371/journal.pone.0003283 |pmc=2553267|display-authors=etal}}
*{{cite journal  |vauthors=Villa C, Venturelli E, Fenoglio C |title=CCL8/MCP-2 association analysis in patients with Alzheimer's disease and frontotemporal lobar degeneration. |journal=J. Neurol. |volume=256 |issue= 8 |pages= 1379–81 |year= 2009 |pmid= 19415413 |doi= 10.1007/s00415-009-5138-y |s2cid=3147096 |display-authors=etal}}
*{{cite journal  |vauthors=Siezen CL, Bont L, Hodemaekers HM |title=Genetic susceptibility to respiratory syncytial virus bronchiolitis in preterm children is associated with airway remodeling genes and innate immune genes. |journal=Pediatr. Infect. Dis. J. |volume=28 |issue= 4 |pages= 333–5 |year= 2009 |pmid= 19258923 |doi= 10.1097/INF.0b013e31818e2aa9 |s2cid=25601837 |display-authors=etal}}
*{{cite journal  |vauthors=Vyshkina T, Sylvester A, Sadiq S |title=CCL genes in multiple sclerosis and systemic lupus erythematosus. |journal=J. Neuroimmunol. |volume=200 |issue= 1–2 |pages= 145–52 |year= 2008 |pmid= 18602166 |doi= 10.1016/j.jneuroim.2008.05.016 |display-authors=etal|pmc=5301077 }}
*{{cite journal  |vauthors=Ruhwald M, Bodmer T, Maier C |title=Evaluating the potential of IP-10 and MCP-2 as biomarkers for the diagnosis of tuberculosis. |journal=Eur. Respir. J. |volume=32 |issue= 6 |pages= 1607–15 |year= 2008 |pmid= 18684849 |doi= 10.1183/09031936.00055508 |display-authors=etal|doi-access=free }}
*{{cite journal  |vauthors=Skibola CF, Bracci PM, Halperin E |title=Polymorphisms in the estrogen receptor 1 and vitamin C and matrix metalloproteinase gene families are associated with susceptibility to lymphoma. |journal=PLOS ONE |volume=3 |issue= 7 |pages= e2816 |year= 2008 |pmid= 18636124 |doi= 10.1371/journal.pone.0002816 |pmc=2474696|display-authors=etal}}
*{{cite journal  |vauthors=Ockinger J, Stridh P, Beyeen AD |title=Genetic variants of CC chemokine genes in experimental autoimmune encephalomyelitis, multiple sclerosis and rheumatoid arthritis. |journal=Genes Immun. |volume=11 |issue= 2 |pages= 142–54 |year= 2010 |pmid= 19865101 |doi= 10.1038/gene.2009.82 |display-authors=etal|doi-access=free }}
*{{cite journal  |vauthors=Xie Z, Zhang J, Wu J |title=Upregulation of mitochondrial uncoupling protein-2 by the AMP-activated protein kinase in endothelial cells attenuates oxidative stress in diabetes. |url=https://archive.org/details/sim_diabetes_2008-12_57_12/page/3222 |journal=Diabetes |volume=57 |issue= 12 |pages= 3222–30 |year= 2008 |pmid= 18835932 |doi= 10.2337/db08-0610 |pmc=2584127|display-authors=etal}}
*{{cite journal  |vauthors=Rajaraman P, Brenner AV, Neta G |title=Risk of meningioma and common variation in genes related to innate immunity. |journal=Cancer Epidemiol. Biomarkers Prev. |volume=19 |issue= 5 |pages= 1356–61 |year= 2010 |pmid= 20406964 |doi= 10.1158/1055-9965.EPI-09-1151 |pmc=3169167|display-authors=etal}}
*{{cite journal  |vauthors=Velez DR, Fortunato S, Thorsen P |title=Spontaneous preterm birth in African Americans is associated with infection and inflammatory response gene variants. |journal=Am. J. Obstet. Gynecol. |volume=200 |issue= 2 |pages= 209.e1–27 |year= 2009 |pmid= 19019335 |doi= 10.1016/j.ajog.2008.08.051 |display-authors=etal|pmc=4829203 }}
*{{cite journal  |vauthors=Dean RA, Cox JH, Bellac CL |title=Macrophage-specific metalloelastase (MMP-12) truncates and inactivates ELR+ CXC chemokines and generates CCL2, -7, -8, and -13 antagonists: potential role of the macrophage in terminating polymorphonuclear leukocyte influx. |journal=Blood |volume=112 |issue= 8 |pages= 3455–64 |year= 2008 |pmid= 18660381 |doi= 10.1182/blood-2007-12-129080 |display-authors=etal|doi-access=free }}
*{{cite journal  |vauthors=Hori T, Naishiro Y, Sohma H |title=CCL8 is a potential molecular candidate for the diagnosis of graft-versus-host disease. |journal=Blood |volume=111 |issue= 8 |pages= 4403–12 |year= 2008 |pmid= 18256320 |doi= 10.1182/blood-2007-06-097287 |pmc=2288733|display-authors=etal}}
*{{cite journal  |vauthors=Schuurhof A, Bont L, Siezen CL |title=Interleukin-9 polymorphism in infants with respiratory syncytial virus infection: an opposite effect in boys and girls. |journal=Pediatr. Pulmonol. |volume=45 |issue= 6 |pages= 608–13 |year= 2010 |pmid= 20503287 |doi= 10.1002/ppul.21229 |display-authors=etal}}
{{refend}}

{{PDB Gallery|geneid=6355}}
{{Hemokini}}
{{Modulatori hemokinskih receptora}}

[[Kategorija:Citokini]]
[[Kategorija:Geni na hromosomu 17]]
[[Kategorija:Ljudski proteini]]
[[Kategorija:Proteomika]]