Parasitism alters sperm quality by inducing stress processes or immunological disorders, which make affect spermatogenesis. In order to evaluate the hypothesis about the immunosuppressive effect of gonadal steroids (males with high testosterone levels would have increased Parasitism susceptibility), our study was conducted assessing testosterone plasmatic concentration and sperm quality in three Spanish chicken (Blue Andaluza, Whitefaced Spanish, Black Castellana) with different parasitism levels by Menacanthus stramineus. In our results, significant differences in sperm quality (P <0.01) and plasmatic testosterone concentrations (P <0.05) between breeds were observed. Negative correlation between parasitism levels and sperm quality was found (P<0.001). In conclusion, high parasitism level by lice determines a reproductive cost in males, manifested in decrease in semen quality
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