Resumen de Utilización de la técnica de pirosecuenciación para el análisis de la comunidad bacteriana del rumen en ovejas alimentadas con dietas suplementadas con microalgas marinas
Tamara Castro Carrera, Pablo Gutiérrez Toral, Gonzalo Hervás Angulo, N. R. McEwan, Leticia Abecia, Pilar de Frutos Fernández, S.E. Girdwood, H.J. Worgan, A. Belenguer
Eleven assaf ewes in lactation were divided in two groups and used to examine, using the pyrosequencing technique, the effect of the dietary inclusion of marine algae (MA) on the structure and composition of the rumen bacterial community. Animals received a total mixed ration based on alfalfa hay and a concentrate (F:C 40:60), supplemented with 25 g of sunflower oil/kg dry matter (DM) plus 0 (Control) or 8 (MA) g of MA/kg DM. After 54 days on treatments, animals were slaughtered and rumen digesta were sampled for microbial analysis by 454 pyrosequencing. Addition of MA altered, as expected, the bacterial community structure and composition. In particular, decreases in the abundance of bacteria that have been previously suggested to be related to rumen biohydrogenation of unsaturated fatty acids (Bacteroidales, Porphyromonadaceae and Ruminococcaceae) were observed, whereas other species of the phylum Proteobacteria, particularly those of the family Succinivibrionaceae, some of which can potentially biohydrogenate, showed an increase. The use of pyrosequencing, a high throughput sequencing methodology, allowed to establish the taxonomy of the bacterial populations affected by the addition of MA to a diet supplemented with sunflower oil in sheep.