Analyses of H2O2 production in time during wound healing

• Autores: Celina Salamanca-Gonzalez, Ilaria Sorrentino, Angie Katherine Molina Oviedo, Eduardo Arevalo-Nuñez de Arenas, Iria Medraño Fernández
• Localización: CASEIB 2023. Libro de Actas del XLI Congreso Anual de la Sociedad Española de Ingeniería Biomédica: Contribuyendo a la salud basada en valor / coord. por Joaquín Roca González, Dolores Ojados González, Juan Suardíaz Muro, 2023, ISBN 978-84-17853-76-1, págs. 206-209
• Idioma: español
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  • Reactive Oxygen Species (ROS) have been traditionally con- sidered as toxic species due to their capacity to damage macromolecules. However, their deleterious effect is lim- ited to high concentrations. At lower levels, further studies have shown that at least hydrogen peroxide (H2O2) acts as a second messenger and results crucial for basic biological functions. Wound healing is one of those: in several animal models, this molecule is emitted in cellular wound margins, leading to neutrophil chemoattraction, while inducing epithe- lial cell migration during skin regeneration. Nevertheless, there are no references to this phenomenon in human ex- perimental set-ups. In this study, human keratinocyte cells (HaCaT), previously modified to express a H2O2-specific ra- tiometric fluorescent sensor (HyPer7) were monitored using live imaging after generating a mechanical-like injury. The time evolution ratio of the probe and the gap area were mea- sured for both cells near the ...