Heat identification by 17β-estradiol and progesterone quantification in individual raw milk samples by enzyme immunoassay

• Anna Domènech ^[2] ; Sara Pich ^[3] ; Anna Arís ^[1] ; Carmen Plasencia ^[3] ; Alex Bach ^[2] ; Alicia Serrano ^[2]
  1. [1] Institució Catalana de Recerca i Estudis Avançats

     Institució Catalana de Recerca i Estudis Avançats

     Barcelona, España

     
  2. [2] Institute of Research and Technology Food and Agriculture
  3. [3] Barcelona Scientific Park Edifici Hélix Applied Research Using Omic Sciences S.L.

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• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 14, Nº. 4, 2011, págs. 10-10
• Idioma: inglés
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• Resumen

  • Background: There is a substantial decline in first-service-pregnancy-rate in dairy cows. In this regard, future prospects are to measure milk hormones on-farm and progesterone levels in milk are not enough to precise ovulation unless connected to other data. The objectives of this study were to investigate whether 17β-estradiol could be measured from individual cow milk samples using a commercially available non-radiolabelled enzyme immunoassay kit (EIA) with no previously reported milk application, and whether those detections could precisely illustrate 17β-estradiol pre-ovulation peak in spite of its limited concentration and short manifestation in milk. Results: Milk sample treatments for progesterone and 17β-estradiol EIA measurements are described. Hormonal profiles from daily milk samples of six different cows were reported and 17β-estradiol pre-ovulation peak was visualized in all cases. Heat detection was possible by EIA using one every 2 days milking samples in almost all studied cases. Only in one case, morning and afternoon milking samples were required to visualize the 17β-estradiol pre-ovulation peak. Conclusions: 17β-estradiol EIA quantification in raw milk is a reliable, rapid, economic and a precise method to describe cow heat along with EIA progesterone determination.