Wikipedia

Endoreduplication

Endoreduplication (also referred to as endoreplication or endocycling) is replication of the nuclear genome in the absence of mitosis, which leads to elevated nuclear gene content and polyploidy. Endoreduplication can be understood simply as a variant form of the mitotic cell cycle (G1-S-G2-M) in which mitosis is circumvented entirely, due to modulation of cyclin-dependent kinase (CDK) activity.[1][2][3][4] Examples of endoreduplication characterised in arthropod, mammalian, and plant species suggest that it is a universal developmental mechanism responsible for the differentiation and morphogenesis of cell types that fulfill an array of biological functions.[1][2] While endoreduplication is often limited to specific cell types in animals, it is considerably more widespread in plants, such that polyploidy can be detected in the majority of plant tissues.[5] Polyploidy and aneuploidy are common phenomena in cancer cells.[6] Given that oncogenesis and endoreduplication likely involve subversion of common cell cycle regulatory mechanisms, a thorough understanding of endoreduplication may provide important insights for cancer biology.

Examples in nature

edit

Endoreduplicating cell types that have been studied extensively in model organisms

Organism Name Cell type Biological function Citation
fly Drosophilia Melanogaster larval tissues (incl. salivary glands) secretion, embryogenesis [7]
fly ovarian follicle, nurse cells nourishment, protection of oocytes [8]
rodent megakaryocyte platelet formation [9]
rodent hepatocyte regeneration [10]
rodent trophoblast giant cell placental development, nourishment of embryo [11]
plant Arabidopsis Thaliana trichome defense from herbivory, homeostasis [12]
plant leaf epidermal cell leaf size, structure [13]
plant endosperm nourishment of embryo [14]
nematode Caenorhabditis elegans hypodermis secretion, body size [15]
nematode intestine unknown [16]

Endoreduplication, endomitosis and polytenization

edit

Endoreduplication, endomitosis and polytenization are three different processes resulting in polyploidization of a cell in a regulated manner. In endoreduplication cells skip M phase completely by exiting the mitotic cell cycle in the G2 phase after completing the S phase several times, resulting in a mononucleated polyploid cell. The cell ends up with twice as many copies of each chromosome per repeat of the S phase.[17] Endomitosis is a type of cell cycle variation where mitosis is initiated, but stopped during anaphase and thus cytokinesis is not completed. The cell ends up with multiple nuclei in contrast to a cell undergoing endoreduplication.[17][18] Therefore depending on how far the cell progresses through mitosis, this will give rise to a mononucleated or binucleated polyploid cell. Polytenization arises with under- or overamplification of some genomic regions, creating polytene chromosomes.[3][4]

 
Endocycling vs. endomitosis

Biological significance

edit

Based on the wide array of cell types in which endoreduplication occurs, a variety of hypotheses have been generated to explain the functional importance of this phenomenon.[1][2] Unfortunately, experimental evidence to support these conclusions is somewhat limited.

Cell differentiation

edit

In developing plant tissues the transition from mitosis to endoreduplication often coincides with cell differentiation and morphogenesis.[19] However it remains to be determined whether endoreduplication and polyploidy contribute to cell differentiation or vice versa. Targeted inhibition of endoreduplication in trichome progenitors results in the production of multicellular trichomes that exhibit relatively normal morphology, but ultimately dedifferentiate and undergo absorption into the leaf epidermis.[20] This result suggests that endoreduplication and polyploidy may be required for the maintenance of cell identity.

Cell/organism size

edit

Cell ploidy often correlates with cell size,[13][15] and in some instances, disruption of endoreduplication results in diminished cell and tissue size [21] suggesting that endoreduplication may serve as a mechanism for tissue growth. Relative to mitosis, endoreduplication does not require cytoskeletal rearrangement or the production of new cell membrane and it often occurs in cells that have already differentiated. As such it may represent an energetically efficient alternative to cell proliferation among differentiated cell types that can no longer afford to undergo mitosis.[22] While evidence establishing a connection between ploidy and tissue size is prevalent in the literature, contrary examples also exist.[19]

Oogenesis and embryonic development

edit

Endoreduplication is commonly observed in cells responsible for the nourishment and protection of oocytes and embryos. It has been suggested that increased gene copy number might allow for the mass production of proteins required to meet the metabolic demands of embryogenesis and early development.[1] Consistent with this notion, mutation of the Myc oncogene in Drosophila follicle cells results in reduced endoreduplication and abortive oogenesis.[23] However, reduction of endoreduplication in maize endosperm has limited effect on the accumulation of starch and storage proteins, suggesting that the nutritional requirements of the developing embryo may involve the nucleotides that comprise the polyploid genome rather than the proteins it encodes.[24]

Buffering the genome

edit

Another hypothesis is that endoreduplication buffers against DNA damage and mutation because it provides extra copies of important genes.[1] However, this notion is purely speculative and there is limited evidence to the contrary. For example, analysis of polyploid yeast strains suggests that they are more sensitive to radiation than diploid strains.[25]

Stress response

edit

Research in plants suggests that endoreduplication may also play a role in modulating stress responses. By manipulating expression of E2fe (a repressor of endocycling in plants), researchers were able to demonstrate that increased cell ploidy lessens the negative impact of drought stress on leaf size.[26] Given that the sessile lifestyle of plants necessitates a capacity to adapt to environmental conditions, it is appealing to speculate that widespread polyploidization contributes to their developmental plasticity

Genetic control of endoreplication

edit

The best-studied example of a mitosis-to-endoreduplication transition occurs in Drosophila follicle cells and is activated by Notch signaling.[27] Entry into endoreduplication involves modulation of mitotic and S-phase cyclin-dependent kinase (CDK) activity.[28] Inhibition of M-phase CDK activity is accomplished via transcriptional activation of Cdh/fzr and repression of the G2-M regulator string/cdc25.[28][29] Cdh/fzr is responsible for activation of the anaphase-promoting complex (APC) and subsequent proteolysis of the mitotic cyclins. String/cdc25 is a phosphatase that stimulates mitotic cyclin-CDK complex activity. Upregulation of S-phase CDK activity is accomplished via transcriptional repression of the inhibitory kinase dacapo. Together, these changes allow for the circumvention of mitotic entry, progression through G1, and entry into S-phase. The induction of endomitosis in mammalian megakaryocytes involves activation of the c-mpl receptor by the thrombopoietin (TPO) cytokine and is mediated by ERK1/2 signaling.[30] As with Drosophila follicle cells, endoreduplication in megakaryocytes results from activation of S-phase cyclin-CDK complexes and inhibition of mitotic cyclin-CDK activity.[31][32]

 
Notch regulation of endocycling

Entry into S-phase during endoreduplication (and mitosis) is regulated through the formation of a prereplicative complex (pre-RC) at replication origins, followed by recruitment and activation of the DNA replication machinery. In the context of endoreduplication these events are facilitated by an oscillation in cyclin E-Cdk2 activity. Cyclin E-Cdk2 activity drives the recruitment and activation of the replication machinery,[33] but it also inhibits pre-RC formation,[34] presumably to ensure that only one round of replication occurs per cycle. Failure to maintain control over pre-RC formation at replication origins results in a phenomenon known as "rereplication" which is common in cancer cells.[2] The mechanism by which cyclin E-Cdk2 inhibits pre-RC formation involves downregulation of APC-Cdh1-mediated proteolysis and accumulation of the protein Geminin, which is responsible for sequestration of the pre-RC component Cdt1.[35][36]

Oscillations in Cyclin E-Cdk2 activity are modulated via transcriptional and post-transcriptional mechanisms. Expression of cyclin E is activated by E2F transcription factors that were shown to be required for endoreduplication.[37][38][39] Recent work suggests that observed oscillations in E2F and cyclin E protein levels result from a negative-feedback loop involving Cul4-dependent ubiquitination and degradation of E2F.[40] Post-transcriptional regulation of cyclin E-Cdk2 activity involves Ago/Fbw7-mediated proteolytic degradation of cyclin E [41][42] and direct inhibition by factors such as Dacapo and p57.[43][44]

Premeiotic endomitosis in unisexual vertebrates

edit

The unisexual salamanders (genus Ambystoma) are the oldest known unisexual vertebrate lineage, having arisen about 5 million years ago.[45] In these polyploid unisexual females, an extra premeiotic endomitotic replication of the genome, doubles the number of chromosomes.[46] As a result, the mature eggs that are produced subsequent to the two meiotic divisions have the same ploidy as the somatic cells of the adult female salamander. Synapsis and recombination during meiotic prophase I in these unisexual females is thought to ordinarily occur between identical sister chromosomes and occasionally between homologous chromosomes. Thus little, if any, genetic variation is produced. Recombination between homeologous chromosomes occurs rarely, if at all.[46]

References

edit
  1. ^ a b c d e Edgar BA, Orr-Weaver TL (2001). "Endoreplication cell cycles: more for less". Cell. 105 (3): 297–306. doi:10.1016/S0092-8674(01)00334-8. PMID 11348589.
  2. ^ a b c d Lee HO, Davidson JM, Duronio RJ (2008). "Endoreplication: polyploidy with purpose". Genes & Development. 23 (21): 2461–77. doi:10.1101/gad.1829209. PMC 2779750. PMID 19884253.
  3. ^ a b Edgar BA, Zielke N, Gutierrez C (2014-02-21). "Endocycles: a recurrent evolutionary innovation for post-mitotic cell growth". Nature Reviews Molecular Cell Biology. 15 (3): 197–210. doi:10.1038/nrm3756. ISSN 1471-0080. PMID 24556841. S2CID 641731.
  4. ^ a b Orr-Weaver TL (2015). "When bigger is better: the role of polyploidy in organogenesis". Trends in Genetics. 31 (6): 307–315. doi:10.1016/j.tig.2015.03.011. PMC 4537166. PMID 25921783.
  5. ^ Galbraith DW, Harkins KR, Knapp S (1991). "Systemic Endopolyploidy in Arabidopsis thaliana". Plant Physiology. 96 (3): 985–9. doi:10.1104/pp.96.3.985. PMC 1080875. PMID 16668285.
  6. ^ Storchova Z, Pellman D (2004). "From polyploidy to aneuploidy, genome instability and cancer". Nature Reviews Molecular Cell Biology. 5 (1): 45–54. doi:10.1038/nrm1276. PMID 14708009. S2CID 11985415.
  7. ^ Hammond MP, Laird CD (1985). "Control of DNA replication and spatial distribution of defined DNA sequences in salivary gland cells of Drosophila melanogaster". Chromosoma. 91 (3–4): 279–286. doi:10.1007/BF00328223. PMID 3920018. S2CID 1515555.
  8. ^ Hammond MP, Laird CD (1985). "Chromosome structure and DNA replication in nurse and follicle cells of Drosophila melanogaster". Chromosoma. 91 (3–4): 267–278. doi:10.1007/BF00328222. PMID 3920017. S2CID 7919061.
  9. ^ Ravid K, Lu J, Zimmet JM, Jones MR (2002). "Roads to polyploidy: The megakaryocyte example". Journal of Cellular Physiology. 190 (1): 7–20. doi:10.1002/jcp.10035. PMID 11807806. S2CID 37297740.
  10. ^ Wang MJ, Chen F, Lau JT, Hu YP (2017-05-18). "Hepatocyte polyploidization and its association with pathophysiological processes". Cell Death & Disease. 8 (5): e2805. doi:10.1038/cddis.2017.167. PMC 5520697. PMID 28518148.
  11. ^ Cross JC (2005). "How to make a placenta: Mechanisms of trophoblast cell differentiation in mice-a review". Placenta. 26: S3–9. doi:10.1016/j.placenta.2005.01.015. PMID 15837063.
  12. ^ Hulskamp M, Schnittger A, Folkers U (1999). Pattern formation and cell differentiation: Trichomes in Arabidopsis as a genetic model system. International Review of Cytology. Vol. 186. pp. 147–178. doi:10.1016/S0074-7696(08)61053-0. ISBN 978-0-12-364590-6. PMID 9770299.
  13. ^ a b Melaragno JE, Mehrotra B, Coleman AW (1993). "Relationship between endopolyploidy and cell size in epidermal tissue of Arabidopsis". The Plant Cell. 5 (11): 1661–8. doi:10.1105/tpc.5.11.1661. JSTOR 3869747. PMC 160394. PMID 12271050.
  14. ^ Sabelli PA, Larkins BA (2009). "The Development of Endosperm in Grasses". Plant Physiology. 149 (1): 14–26. doi:10.1104/pp.108.129437. PMC 2613697. PMID 19126691.
  15. ^ a b Flemming AJ, Shen Z, Cunha A, Emmons SW, Leroi AM (2000). "Somatic polyploidization and cellular proliferation drive body size evolution in nematodes". PNAS. 97 (10): 5285–90. Bibcode:2000PNAS...97.5285F. doi:10.1073/pnas.97.10.5285. PMC 25820. PMID 10805788.
  16. ^ Hedgecock EM, White JG (January 1985). "Polyploid tissues in the nematode Caenorhabditis elegans". Developmental Biology. 107 (1): 128–133. doi:10.1016/0012-1606(85)90381-1. ISSN 0012-1606. PMID 2578115.
  17. ^ a b Zielke N, Edgar BA, DePamphilis ML (2013-01-01). "Endoreplication". Cold Spring Harbor Perspectives in Biology. 5 (1): a012948. doi:10.1101/cshperspect.a012948. ISSN 1943-0264. PMC 3579398. PMID 23284048.
  18. ^ Shu Z, Row S, Deng WM (June 2018). "Endoreplication: The Good, the Bad, and the Ugly". Trends in Cell Biology. 28 (6): 465–474. doi:10.1016/j.tcb.2018.02.006. ISSN 0962-8924. PMC 5962415. PMID 29567370.
  19. ^ a b Inze D, De Veylder L (2006). "Cell cycle regulation in plant development". Annual Review of Genetics. 40: 77–105. doi:10.1146/annurev.genet.40.110405.090431. PMID 17094738.
  20. ^ Bramsiepe J, Wester K, Weinl C, Roodbarkelari F, Kasili R, Larkin JC, Hulskamp M, Schnittger A (2010). Qu LJ (ed.). "Endoreplication Controls Cell Fate Maintenance". PLOS Genetics. 6 (6): e1000996. doi:10.1371/journal.pgen.1000996. PMC 2891705. PMID 20585618.
  21. ^ Lozano E, Saez AG, Flemming AJ, Cunha A, Leroi AM (2006). "Regulation of growth by ploidy in Caenorhabditis elegans". Current Biology. 16 (5): 493–8. Bibcode:2006CBio...16..493L. doi:10.1016/j.cub.2006.01.048. PMID 16527744.
  22. ^ Kondorosi E, Roudier F, Gendreau E (2000). "Plant cell-size control: Growing by ploidy?". Current Opinion in Plant Biology. 3 (6): 488–492. Bibcode:2000COPB....3..488K. doi:10.1016/S1369-5266(00)00118-7. PMID 11074380.
  23. ^ Maines JZ, Stevens LM, Tong X, Stein D (2004). "Drosophila dMyc is required for ovary cell growth and endoreplication". Development. 131 (4): 775–786. doi:10.1242/dev.00932. PMID 14724122.
  24. ^ Leiva-Neto JT, Grafi G, Sabelli PA, Dante RA, Woo YM, Maddock S, Gordon-Kamm WJ, Larkins BA (2004). "A Dominant Negative Mutant of Cyclin-Dependent Kinase A Reduces Endoreduplication but Not Cell Size or Gene Expression in Maize Endosperm". The Plant Cell. 16 (7): 1854–69. doi:10.1105/tpc.022178. PMC 514166. PMID 15208390.
  25. ^ Mortimer RK (1958). "Radiobiological and genetic studies on a polyploid series (haploid to hexaploid) of Saccharomyces cerevisiae". Radiation Research. 9 (3): 312–326. Bibcode:1958RadR....9..312M. doi:10.2307/3570795. JSTOR 3570795. PMID 13579200. S2CID 37053611.
  26. ^ Cookson SJ, Radziejwoski A, Granier C (2006). "Cell and leaf size plasticity in Arabidopsis: what is the role of endoreplication?". Plant, Cell and Environment. 29 (7): 1273–83. doi:10.1111/j.1365-3040.2006.01506.x. PMID 17080949.
  27. ^ Deng WM, Althauser C, Ruohala-Baker H (2001). "Notch-Delta signaling induces a transition from mitotic cell cycle to endocycle in Drosophila follicle cells". Development. 128 (23): 4737–46. doi:10.1242/dev.128.23.4737. PMID 11731454.
  28. ^ a b Shcherbata HR, Althauser C, Findley SD, Ruohola-Baker H (2004). "The mitotic-to-endocycle switch inDrosophila follicle cells is executed by Notch-dependent regulation of G1/S, G2/M and M/G1 cell-cycle transitions". Development. 131 (13): 3169–81. doi:10.1242/dev.01172. PMID 15175253.
  29. ^ Schaeffer V, Althauser C, Shcherbata HR, Deng WM, Ruohola-Baker H (2004). "Notch-dependent Fizzy-related/Hec1/Cdh1 expression is required for the mitotic-to-endocycle transition in Drosophila follicle cells". Current Biology. 14 (7): 630–6. Bibcode:2004CBio...14..630S. doi:10.1016/j.cub.2004.03.040. hdl:11858/00-001M-0000-002D-1B8D-3. PMID 15062106. S2CID 18877076.
  30. ^ Kaushansky K (2005). "The molecular mechanisms that control thrombopoiesis". The Journal of Clinical Investigation. 115 (12): 3339–47. doi:10.1172/JCI26674. PMC 1297257. PMID 16322778.
  31. ^ Garcia P, Cales C (1996). "Endoreplication in megakaryoblastic cell lines is accompanied by sustained expression of G1/S cyclins and downregulation of cdc25c". Oncogene. 13 (4): 695–703. PMID 8761290.
  32. ^ Zhang Y, Wang Z, Ravid K (1996). "The cell cycle in polyploid megakaryocytes is associated with reduced activity of cyclin B1-dependent cdc2 kinase". Journal of Biological Chemistry. 271 (8): 4266–72. doi:10.1074/jbc.271.8.4266. PMID 8626773.
  33. ^ Su TT, O'Farrell PH (1998). "Chromosome Association of Minichromosome Maintenance Proteins in Drosophila Endoreplication Cycles". Journal of Cell Biology. 140 (3): 451–460. doi:10.1083/jcb.140.3.451. PMC 2140170. PMID 9456309.
  34. ^ Arias EE, Walter JC (2004). "Strength in numbers: Preventing rereplication via multiple mechanisms in eukaryotic cells". Genes & Development. 21 (5): 497–518. doi:10.1101/gad.1508907. PMID 17344412.
  35. ^ Narbonne-Reveau K, Senger S, Pal M, Herr A, Richardson HE, Asano M, Deak P, Lilly MA (2008). "APC/CFzr/Cdh1 promotes cell cycle progression during the Drosophila endocycle". Development. 135 (8): 1451–61. doi:10.1242/dev.016295. PMID 18321983.
  36. ^ Zielke N, Querings S, Rottig C, Lehner C, Sprenger F (2008). "The anaphase-promoting complex/cyclosome (APC/C) is required for rereplication control in endoreplication cycles". Genes & Development. 22 (12): 1690–1703. doi:10.1101/gad.469108. PMC 2428065. PMID 18559483.
  37. ^ Duronio RJ, O'Farrell PH (1995). "Developmental control of the G1 to S transition in Drosophila: Cyclin E is a limiting downstream target of E2F". Genes & Development. 9 (12): 1456–68. doi:10.1101/gad.9.12.1456. PMID 7601350.
  38. ^ Duronio RJ, O'Farrell PH, Xie JE, Brook A, Dyson N (1995). "The transcription factor E2F is required for S phase during Drosophila embryogenesis". Genes & Development. 9 (12): 1445–55. doi:10.1101/gad.9.12.1445. PMID 7601349.
  39. ^ Duronio RJ, Bonnette PC, O'Farrell PH (1998). "Mutations of the Drosophila dDP, dE2F, and cyclin E Genes Reveal Distinct Roles for the E2F-DP Transcription Factor and Cyclin E during the G1-S Transition". Molecular and Cellular Biology. 18 (1): 141–151. doi:10.1128/MCB.18.1.141. PMC 121467. PMID 9418862.
  40. ^ Shibutani ST, de la Cruz AF, Tran V, Turbyfill WJ, Reis T, Edgar BA, Duronio RJ (2008). "Intrinsic negative cell cycle regulation provided by PIP box- and Cul4Cdt2-mediated destruction of E2f1 during S phase". Developmental Cell. 15 (6): 890–900. doi:10.1016/j.devcel.2008.10.003. PMC 2644461. PMID 19081076.
  41. ^ Koepp DM, Schaefer LK, Ye X, Keyomarsi K, Chu C, Harper JW, Elledge SJ (2001). "Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligase". Science. 294 (5540): 173–7. Bibcode:2001Sci...294..173K. doi:10.1126/science.1065203. PMID 11533444. S2CID 23404627.
  42. ^ Moberg KH, Bell DW, Wahrer DC, Haber DA, Hariharan IK (2001). "Archipelago regulates cyclin E levels in Drosophila and is mutated in human cancer lines". Nature. 413 (6853): 311–6. doi:10.1038/35095068. PMID 11565033. S2CID 4372821.
  43. ^ de Nooij JC, Graber KH, Hariharan IK (2001). "Expression of cyclin-dependent kinase inhibitor Dacapo is regulated by cyclin E". Mechanisms of Development. 97 (1–2): 73–83. doi:10.1016/S0925-4773(00)00435-4. PMID 11025208.
  44. ^ Ullah Z, Kohn MJ, Yagi R, Vassilev LT, DePamphilis ML (2008). "Differentiation of trophoblast stem cells into giant cells is triggered by p57/Kip2 inhibition of CDK1 activity". Genes & Development. 22 (21): 3024–36. doi:10.1101/gad.1718108. PMC 2577795. PMID 18981479.
  45. ^ Bi K, Bogart JP (2010). "Time and time again: unisexual salamanders (genus Ambystoma) are the oldest unisexual vertebrates". BMC Evol. Biol. 10 (1): 238. Bibcode:2010BMCEE..10..238B. doi:10.1186/1471-2148-10-238. PMC 3020632. PMID 20682056.
  46. ^ a b Bi K, Bogart JP (2010). "Probing the meiotic mechanism of intergenomic exchanges by genomic in situ hybridization on lampbrush chromosomes of unisexual Ambystoma (Amphibia: Caudata)". Chromosome Res. 18 (3): 371–82. doi:10.1007/s10577-010-9121-3. PMID 20358399. S2CID 2015354.
Retrieved from "https://en.wikipedia.org/w/index.php?title=Endoreduplication&oldid=1245410045"