The film was largely financed through Harry Cushing, but just prior to the start of filming a letter of credit from a film company was not accepted by the Italian banks. The final last-minute $90,000 needed to make the film was obtained from Roger Corman's New World Pictures. The financiers of the film chose Rosalba Neri as the lead role of Tania Frankenstein in the film.
Rob Zombie sampled the line "Who is this irresistible creature who has an insatiable love for the dead?" from the trailer for his song "Living Dead Girl."
Lady Frankenstein was distributed theatrically in Italy by Alexia on October 22, 1971. It grossed a total of 139,683,000 Italian lire domestically. It was released in the United States in March 1972 where it was distributed by New World Pictures.
Italian censorship visa # 59112 delivered on 10-18-1971.