Laboratory of Plant Development and Interactions :: Movies
Videos
Peroxisome movement along F-actin
The movement of YFP-PTS1 highlighted peroxisomes (yellow) along filamentous GFP-FABD-mTalin highlighted green actin visualized in a plant cell. Mathur et al. Plant Physiology 128,1031-1045,2002. © J Mathur.
Cortical Microtubules in a leaf
The dynamic behaviour of cortical microtubules visualized in leaf epidermal cells of Arabidopsis.An artificially placed time lapse video of AtEB1b:GFP labelled growing ends shows the dynamic behaviour of microtubules that make up the cortical array. © J Mathur.
Microtubule dynamics
The dynamic behaviour of cortical microtubules visualized in an onion epidermal cell. Pseudo-coloured red and a green microtubule glide past each other; another green one exhibits rapid depolymerization or catastrophe, while a yellow coloured microtubule exhibits plus end extension or growth. © J Mathur.
EB1b labeling of microtubule plus ends
Green fluorescent microtubule plus ends visualized in leaf pavement cells of stably transformed Arabidopsis plants. Mathur et al. 2003. 13.1991-1998. Current Biology.© J Mathur.
Photoconversion of plastids
Green fluorescent chloroplasts appear to interact via their stromules (tubular extensions). However, use of a photoconvertible probe consisting of tpFNR fused to mEosFP allows differential colouring (red and green) and shows that the seeming inter-connectivity between plastids does not exist.Schattat et al. Plant Cell. 2012, 24, 1465 -1477.© J Mathur.
Plastid associated ER
A close association exists between plastids and the endoplasmic reticulum (ER). The time-lapse movie shows RFP-targeted to the ER and chlorophyll fluorescence (false coloured blue). Schattat et al. 2011. Plant Physiol.155,1667-1677. © J Mathur.
Fusion of Mitochondria
Differential colouring of mitochondria achieved by targeting a photoconvertible mEos fluorescent protein to them allowed visualization of mitochondrial fusion in tobacco leaf epidermal cells. Schattat et al. Plant Cell. 2012, 24, 1465 -1477.© J Mathur.
Arabidopsis Seed germination
A time-lapse movie showing germination of Arabidopsis thaliana seeds. Credit: Dr. Martin Schattat. © J Mathur.
Arabidopsis Plant Development
A time-lapse movie stitched together from images captured every 10 minutes over 7 days shows the development of Arabidopsis thaliana plants from a rosette to the flowering stage. Credit: Dr. Nina K. Barabas. © J Mathur.
Arabidopsis Development- A flowering plant
A time-lapse movie stitched together from images captured every 10 minutes shows the rotational dance of a growing inflorescence of Arabidopsis thaliana plants. Credit: Dr. Nina K. Barabas. © J Mathur.
Trichome Development in any1
Time-lapse movie recorded over 72 hours to show the development of leaf epidermal hairs (trichomes) in Arabidopsis thaliana. The trichomes fluoresce green due to cell specific GFP-expression through the use of a GLABRA2 promoter.Credit: Michael Wozny © J Mathur.
Synchronous Collet hair Development
Time-lapse movie recorded over 50 hours shows the synchronous development of collet hairs in Arabidopsis thaliana. Note the movement of nuclei in the tip growing cells.Sliwinska et al. J. Expt. Bot. 2012.63(11)4165-4178.© J Mathur.
Cytoplasmic streaming in a swollen trichome
Time-lapse movie showing cytoplasmic streaming in an isotropically expanded leaf epidermal trichome cell in the any1 mutant of Arabidopsis.© J Mathur.
Cytoplasmic streaming in a hypocotyl cell
Time-lapse movie showing cytoplasmic streaming in a hypocotyl cell in a transgenic Arabidopsis seedling expressing GFP:EB1. Mathur et al. Curr. Biol. 2003. © J Mathur.
Organelle movement in plant cells
A time-lapse movie showing the movement of endoplasmic reticulum (ER), ER-bodies and plastids in Arabidopsis cells.Some plastids exhibit extensions called stromules.© J Mathur.
Movement of ER bodies in Arabidopsis cells
A short time-lapse movie showing the movement of spindle shaped, RFP labeled, ER-bodies and plastids in Arabidopsis cells.Some plastids exhibit extensions called stromules.© J Mathur.
Peroxules:Extensions of peroxisomes
Peroxisomes extend thin tubules named 'peroxules' in response to transient increase in sub-cellular ROS. Sinclair et al. Plant J.2009.59, 231-242. © J Mathur.
Peroxisome-ER association
In response to ROS-stress peroxisomes (green)elongate before undergoing fission.The behaviour of elongated peroxisomes closely follows that of contiguous ER (shown in red) and suggests that th two organelles might be linked. Sinclair et al. Plant J.2009.59, 231-242. © J Mathur.
Cytoskeletal elements at the plant cell cortex
A short time-lapse movie visualizing actin filaments (F-actin) highlighted in green by targeted GFP:FABD-mTalin and RFP:MBD-MAP4 highlighted microtubules (red) in the cell cortex.© J Mathur.
F-actin in the wurm mutant
Filamentous actin (F-actin) highlighted by expressing a targeted GFP:FABD-mTalin shows fine and dense F-actin organization in a distorted trichome cell of the wurm mutant of Arabidopsis.Mathur et al. 2003. Plant Cell.15,1632-1645.© J Mathur.
Branching of a stromule
A time-lapse movie showing a kinked-branching stromule from an epidermal plastid expressing tpFNR:GFP. Schattat et al. 2011. Plant Physiol.155,1667-1677. © J Mathur.