A primer on metagenomics

JC Wooley, A Godzik, I Friedberg - PLoS computational biology, 2010 - journals.plos.org
… various levels of metagenomic annotation. … metagenome size and the handling of associated
metadata. Finally, we will review some studies highlighting the advantages of metagenomic-…

A primer on metagenomics and next‐generation sequencing in fish gut microbiome research

TK Johny, RM Puthusseri, SG Bhat - Aquaculture Research, 2021 - Wiley Online Library
… of and prospects of metagenomics and amplicon and … First of all, the construction and screening
of metagenomic libraries … functional metagenomics, the sequence-based metagenomics

Better primer design for metagenomics applications by increasing taxonomic distinguishability

M Jaric, J Segal, E Silva-Herzog, L Schneper… - BMC proceedings, 2013 - Springer
Current methods of understanding microbiome composition and structure rely on accurately
estimating the number of distinct species and their relative abundance. Most of these …

Viral metagenomics

EL Delwart - Reviews in medical virology, 2007 - Wiley Online Library
… In the recent metagenomic studies viral particles from … Metagenomic approaches to viral
characterisation have … Viral metagenomic approaches provide novel opportunities to generate …

Coverage evaluation of universal bacterial primers using the metagenomic datasets

DP Mao, Q Zhou, CY Chen, ZX Quan - BMC microbiology, 2012 - Springer
… Additionally, most studies do not account for the positional effect of single primer-template
mismatches. In this study, we used 7 metagenomic datasets as well as the Ribosomal …

MIPE: A metagenome-based community structure explorer and SSU primer evaluation tool

B Zou, JF Li, Q Zhou, ZX Quan - PLoS One, 2017 - journals.plos.org
… Because metagenomic and metatranscriptomic sequences are generated … metagenome
datasets to evaluate universal primers for the bacterial 16S rRNA gene and found that primer

Beyond research: a primer for considerations on using viral metagenomics in the field and clinic

RJ Hall, JL Draper, FGG Nielsen, BE Dutilh - Frontiers in microbiology, 2015 - frontiersin.org
Powered by recent advances in next-generation sequencing technologies, metagenomics
has already unveiled vast microbial biodiversity in a range of environments, and is …

A bioinformatician's guide to metagenomics

V Kunin, A Copeland, A Lapidus… - Microbiology and …, 2008 - Am Soc Microbiol
… For the purposes of this review, we define metagenomics as the application of shotgun
sequencing to DNA obtained directly from an environmental sample or series of related samples, …

Metagenomics-a guide from sampling to data analysis

T Thomas, J Gilbert, F Meyer - Microbial informatics and experimentation, 2012 - Springer
metagenomics, with particular emphasis on the steps involved in a typical sequence-based
metagenome … Clearly, any kind of metagenomic dataset will benefit from the rich information …

The impact of primer design on amplicon-based metagenomic profiling accuracy: detailed insights into bifidobacterial community structure

L Mancabelli, C Milani, GA Lugli, F Fontana, F Turroni… - Microorganisms, 2020 - mdpi.com
metagenomic primers. In detail, PP39 (Probio_Uni/Probio_Rev), PP41 (341F/534R), and
PP72 (970F/1050R) were the most suitable primer … A total of 75 primer pairs were included in …