Methylotrophic bacteria utilize methanol and other reduced one-carbon compounds as their
sole source of carbon and energy. For this purpose, these bacteria evolved a number of …

The pyridine nucleotides NAD(H) and NADP(H) play major roles in the formation of by-products.
To analyse how Saccharomyces cerevisiae (S. cerevisiae) metabolism during growth …

Metabolomics is a founding pillar of quantitative biology and a valuable tool for studying
metabolism and its regulation. Here we present a workflow for metabolomics in microplate …

Metabolism of an organism is composed of hundreds to thousands of interconnected biochemical
reactions responding to environmental or genetic constraints. This metabolic network …

Advances in metabolic engineering are enabling the creation of a large number of cell factories.
However, high-throughput platforms do not yet exist for rapidly analyzing the metabolic …

We recently showed that expressing an H 2 O-NADH oxidase in Saccharomyces cerevisiae
drastically reduces the intracellular NADH concentration and substantially alters the …

Highlights • 13 C-fluxomics provides quantification of metabolic activity in microorganisms. •
Increasing flux analysis capacity has the potential to accelerate strain design. • HT 13 C-…

Methanol is a sustainable substrate for biotechnology. In addition to natural methylotrophs,
metabolic engineering has gained attention for transfer of methylotrophy. Here, we …

Background Malate is a C4-dicarboxylic acid widely used as an acidulant in the food and
beverage industry. Rational engineering has been performed in the past for the development …

White biotechnology is a term that is now often used to describe the implementation of
biotechnology in the industrial sphere. Biocatalysts (enzymes and microorganisms) are the key …