Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry

JN Adkins, SM Varnum, KJ Auberry, RJ Moore… - Molecular & Cellular …, 2002 - ASBMB
Blood serum is a complex body fluid that contains various proteins ranging in concentration
over at least 9 orders of magnitude. Using a combination of mass spectrometry technologies …

Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome

SM Varnum, DN Streblow, ME Monroe, P Smith… - Journal of …, 2004 - Am Soc Microbiol
Human cytomegalovirus (HCMV), a member of the herpesvirus family, is a large complex
enveloped virus composed of both viral and cellular gene products. While the sequence of the …

Specific DNA binding of GAL4, a positive regulatory protein of yeast

E Giniger, SM Varnum, M Ptashne - Cell, 1985 - Elsevier
We show by the following series of experiments that the yeast positive regulatory protein
GAL4 binds to four sites in the upstream activating sequence UAS G to activate transcription of …

Enhanced detection of low abundance human plasma proteins using a tandem IgY12-SuperMix immunoaffinity separation strategy

…, T Liu, X Zhang, HM Mottaz, SM Varnum… - Molecular & Cellular …, 2008 - ASBMB
The enormous dynamic range of human bodily fluid proteomes poses a significant challenge
for current MS-based proteomics technologies as it makes it especially difficult to detect low …

Targeted Mutagenesis by Duplication Insertion in the Radioresistant Bacterium Deinococcus radiodurans: Radiation Sensitivities of Catalase (katA) and Superoxide Dismutase …

LM Markillie, SM Varnum, P Hradecky… - Journal of …, 1999 - Am Soc Microbiol
Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing
radiation. A simple and general targeted mutagenesis method was developed to generate …

[HTML][HTML] High dynamic range characterization of the trauma patient plasma proteome

…, A Kaushal, ME Monroe, SM Varnum… - Molecular & Cellular …, 2006 - Elsevier
Although human plasma represents an attractive sample for disease biomarker discovery,
the extreme complexity and large dynamic range in protein concentrations present significant …

The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus

PA Krieg, SM Varnum, WM Wormington… - Developmental …, 1989 - Elsevier
A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences
that are expressed early in development. We find that the mRNA encoding translation …

Proteomic characterization of nipple aspirate fluid: identification of potential biomarkers of breast cancer

SM Varnum, CC Covington, RL Woodbury… - Breast cancer research …, 2003 - Springer
Mammary ductal cells are the origin for 70–80% of breast cancers. Nipple aspirate fluid (NAF)
contains proteins directly secreted by the ductal and lobular epithelium in non-lactating …

Comparative proteomics and pulmonary toxicity of instilled single-walled carbon nanotubes, crocidolite asbestos, and ultrafine carbon black in mice

…, AR Murray, ER Kisin, SM Varnum… - Toxicological …, 2011 - academic.oup.com
Reflecting their exceptional potential to advance a range of biomedical, aeronautic, and
other industrial products, carbon nanotube (CNT) production and the potential for human …

Combined statistical analyses of peptide intensities and peptide occurrences improves identification of significant peptides from MS-based proteomics data

…, JM Jacobs, TO Metz, SM Varnum… - Journal of proteome …, 2010 - ACS Publications
Liquid chromatography−mass spectrometry-based (LC−MS) proteomics uses peak intensities
of proteolytic peptides to infer the differential abundance of peptides/proteins. However, …