OME-3DR
3D cell reconstruction and quantitative analysis based on OME data
For big OME data analysis, we integrate commonly used quantitative methods, describe our novel strategies to quantify and analyze biological markers related to the cell or organelle spatial-coordinate model, and present open-source OME-3-Dimensional Reconstruction (OME-3DR), a flexible, programmable and batch-oriented tool based on OME data, for reconstructing 3-dimensional (3D) spatial conformations and conducting further analyses, such as the identification, counting, localization and tracking of bio-imaging markers, calculation of model-contour parameters for association analyses, establishing spatial-coordinate system, 3D co-localization analyses and so on. OME-3DR is well modularized (for automation, three main menus integrate hundreds of sub-functions), easy to use and ingenious; indeed, after setting the corresponding parameters, batch processing is relatively straightforward when beginning the 3D model reconstruction...