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Getting Started

Get up and running quickly with this brief tour of the Geneious Prime interface.
Watch this series to get started with your molecular biology and sequence analysis in Geneious Prime.
Watch this series to get started with your antibody analysis in Geneious Biologics.

Next Generation Sequencing

Use this practical exercise to perform a de novo assembly of short-read NGS data and assemble circular contigs.
With this practical exercise, perform a reference assembly with NGS data and call SNPs on the assembled contig.
Assemble, filter and analyze an NGS amplicon metagenomic data set in Geneious Prime with this practical exercise.
Practice calculating normalized expression measures from RNA-Seq data using the Geneious expression analysis tool.
Use DESeq2 to compare expression levels for two sample conditions with replicates, in this practical exercise.
Practice using the Sequence Classifier by classifying mitochondrial sequences obtained from subfossil bones.

Sanger Sequencing and Microsatellites

Learn how to assemble chromatograms, edit bidirectional DNA sequences, and extract high-quality consensus sequences.
Practice using the Microsatellite plugin to fit a ladder, call peaks, bin alleles and produce a table of genotypes.

Learn Sequence Analysis

Learn the basics of DNA sequencing with this introductory article on Sanger, Next Generation and Long-read methods.
Learn the basics with this introductory guide to assembling DNA sequences with de novo and map to reference.
Learn the basics of sequence alignment with this overview on the different methods used to align sequences.
Learn the basics of phylogenetics with this overview of phylogenetic treesm how they work and how to build them.
Learn the basics in Molecular Cloning with this overview article that covers restriction enzyme cloning to golden gate cloning.

Alignments and Tree Building

Practice aligning sequences, building a phylogenetic tree, and viewing and manipulating the tree.
Use this practical exercise to align pairs of DNA and protein sequences using dot plots and alignment algorithms.
Practice using the Mauve plugin to align genomes, complete genomes, and order draft genomes against a reference.
Practice how to annotate plant DNA sequences using BLAST and multiple alignment. Read a phylogenetic tree.

Annotate and Visualize

Use the Transfer Annotations tool to transfer features to a reference or consensus sequence in an alignment or assembly.
Practice transferring annotations between sequences within an alignment using the "Copy To..." function.
Practice how to transfer homologous annotations from a custom feature database using the "Annotate From" tool.
With this practical exercise, explore the controls in the 3D structure viewer. Visualize active sites in a protein structure.

Molecular Cloning

Practice simulating restriction cloning. Ligate PCR product into an expression vector and confirm CDS in-frame fusion.
Practice simulating Gibson Assembly of a single insert, and perform batch cloning from a sequence list.
Practice simulating Golden Gate cloning. Automatically design primers to generate overhangs for parts assembly.
Group reads into clones, map clones to a reference and automate validation of sequences with the cloning validation tool.
Practice simulating gateway cloning. Automatically design attB-linked primers, and the BP and LR reactions.
Practice simulating multisite Gateway cloning. Choose attB sites, design primers, and simulate BP and LR reactions.
In this practical exercise, learn how to simulate TOPO™ cloning, using either TA, Blunt or Directional methods.

Primer Design and CRISPR

Practice how to import, design and test oligonucleotide primers, primer-pairs and primer-pair/probe combinations.
Practice analyzing CRISPR editing results, preparing sequences, setting up the analysis and interpreting results.
Practice finding and annotating CRISPR sites in a sequence. Check against a genome for off-target binding sites.

BLAST, NCBI & GenBank

A practical exercise to BLAST your sequence against GenBank or custom databases to find similar sequences.
Practice how to access information stored in the GenBank database through the Geneious interface.
Practice formatting sequences and alignments for submission to GenBank with the GenBank Submission tool.

Automation & Customization

Practice setting up an automatic workflow to run a pipeline from Sanger sequences to a phylogenetic tree.

Antibody Discovery

Watch this series to get started with your antibody analysis in Geneious Biologics.
Deepen your understanding of key concepts in antibody discovery and master the broad range of Geneious Biologics tools with this in-depth video series.
Get started with Geneious today