When Charlie drives into Greenville (12:41), she has the cigarette in her mouth, and there is about 1cm left. When they pull over to stop, she has the cigarette in her hand, and there is about 3-4cm left.
During the car chase toward the end of the episode, the weather varies wildly, from foggy to sunny to overcast and back again, often changing between changes in camera angles which take place during conversations, and so cannot be explained by time passing.
When Charlie is picked up by the trucker, she throws the FBI jacket on the side of the road. When the trucker pulls off the jacket is missing form the side of the road.
When Charlie stops at a diner in Indiana, a man is shown playing a slot or poker machine, which is illegal in the state.
Impossible for iguana to be in the car while Charlie is away for less than 2 minutes she beeps the horn and in that time kill everyone guarding the witness and the witness outside and inside the house.
Cia and NSA have no interest in a local gang boss and don't control witness safety us Marshall's do.
Charlie constantly calls out lies by hearing them but the big reveal is that iguana could control her physical responses.