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During each episode, a 15-second presentation appears, asking the public for help in finding real-life missing persons. The FBI provides a picture and descriptive information about the missing person to be displayed with a voice-over message recorded by one of the series stars.

Since the inception of the agency, FBI agents travel in pairs when interview subjects. This is both to have a witness to the interview as well as for protection in case unexpected things occur. Multiple times during the show, single agents are shown talking with subjects alone, even in their homes or places unfamiliar to the agents.

Amongst the opening credits, for a brief moment the words "Amber Alert" are flashed on the screen. The Amber Alert system was formally inaugurated in the state of California, July 31, 2002. It drew both national and international recognition in its success in aiding authorities in their search for abducted children. In less than one year, the California Amber Alert system has been credited with aiding in the rescue of over a dozen children. The intial system was formulated in Texas in 1996, and is named after 9-year-old Amber Hagerman who was kidnapped and murdered. As of April 10, 2003 a bill creating a national Amber Alert system has passed in the House of Representitives, and unanimously approved in the Senate. "Without A Trace" (2002) aired its first episode shortly after a summer littered with nationally covered child kidnappings including the famous Elizabeth Smart abduction. The show aired Elizabeth's profile following its November 21, 2002 episode, In Extrimis. All Profiles are chosen by the FBI, not the show's producers.

Featured in The 55th Annual Primetime Emmy Awards (2003)