- His character in Gremlins 2: The New Batch (1990) was originally written as a corrupt, deeply unpleasant businessman. Glover, who was getting sick of being cast as villains, instead chose to portray him as an immensely likable and friendly, if eccentric and immature, character.
- Has played the father of two television supervillains. He played Lex Luthor's father on Smallville (2001) and the father of Sylar on Heroes (2006).
- Aside from his theatrical endeavours, he is actively involved with the Alzheimer's Association.
- During John Glover's interview on "Inside of You with Michael Rosenbaum" (hosted by the actor who played Glover's character's son on the TV show "Smallville"), Glover revealed that during the 1970s he had a one-night-stand in London with Queen lead singer Freddie Mercury.
- Won Broadway's 1995 Tony Award as Best Actor (Featured Role - Play) for the dual roles of John Jeckyll and James Jeckyll in Terence McNally's "Love! Valour! Compassion!", parts he recreated in the film version of the same title, Love! Valour! Compassion! (1997).
- Visits his alma mater Towson University in Maryland regularly to work with drama students in the Fine Arts College.
- Tom Welling said that his favorite part of season four of Smallville (2001) was working on the episode in which Clark and Lionel switch bodies as it allowed him to work closely with John Schneider, whom he greatly admires.
- Has been in four separate productions associated with DC comics. First as the Riddler on Batman: The Animated Series (1992), Dr. Jason Woodrue in Batman & Robin (1997), Lionel Luthor on Smallville (2001), then as Sivana's father in Shazam! (2019).
- Glover married sculptor Adam Kurtzman in 2016, having been together since 1993.
- In 1977, he played two films that were nominated for the Academy Award for Best Picture: Annie Hall (1977) and Julia (1977). The former won the award.
- Attended and graduated from Towson State Teacher's College (now Towson University). He later received an honorary Master's degree.
- Nominated for at 2009 Tony Award for Best Performance by a Featured Actor in a Play for playing Lucky in "Waiting for Godot" by Samuel Beckett at Studio 54.
- Made Broadway debut in "The Selling of the President" in 1972.
- He was awarded the 1987 Drama Logue Award for Outstanding Performance for "The Traveler" at the Mark Taper Forum Theatre in Los Angeles, California.
- Close friends with actress Blair Brown.
- His father, John Soursby Glover Sr., was a television salesman.
- For his role as an assassin in NBC's Grass Roots (1992) he spent four hours daily in the make-up chair.
- He splits time between Los Angeles, California and Vancouver, British Columbia, where the WB Television series Smallville (2001) was filmed.
- Made his stage debut in "Look Homeward, Angel" at the Barter Theatre in Virginia in 1963.
- For three college summers, he worked at a small theater in southwestern Virginia.
- Attended and graduated from Wicomico High School in Saliabury, Maryland.
- He is a lifelong Democrat.
- Moved to New York City in 1967.
- Parents are John Soursby Glover Sr. and Asenath Cade Mullins.
- Studied acting with Milton Katselas at the Beverly Hills Playhouse.
- He was awarded the 1987 Los Angeles Drama Critics Circle Award for Distinguished Lead Performance for "The Traveler" at the Mark Taper Forum Theatre in Los Angeles, California.
- Born on exactly the same date as David Rasche (of Sledge Hammer! (1986) fame).
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