There is a scene where a tv in the background is discussing strange activity on mars. This is a reference to the introduction of the Martian Manhunter.
When Jim Gordon is giving a speech about Batman's disappearance, the news channel on the bottom right says "News 52". It is a reference to the new 52 line of DC Comics.
This is the first appearance of Batwing (Luke Fox) in media outside of the comic book medium.
The film is based on the comics Batman: Battle for the Cowl and Batman: Incorporated.
This is Jason O'Mara's 5th time voicing Batman in an animated film.