The Pope/Michelangelo sketch was based on a historical incident. The Renaissance artist Paolo Veronese really did paint a "Last Supper" with extraneous contemporary elements. The Inquisition investigated and advised him to remove those elements. Instead, he really did just rename it - to "Feast in the House of Levi".
Eric Idle sang the "Lumberjack Song" in this movie, even though Sir Michael Palin sang it on Monty Python's Flying Circus (1969).
This marks the last time The Ministry of Silly Walks sketch was performed by the troupe. John Cleese had a hip and knee replacement in 2010, thus making Cleese unable to perform the fan favorite Silly Walks anymore.
The "Custard Pie Lecture" sketch predates Monty Python. It was written by Sir Michael Palin and Terry Jones while they were students at Oxford, and was their first writing collaboration.
This marked the first occasion where the Monty Python fan favorite "Sit on my Face" was performed live.