When Lucy is tazing Gru he drops his freeze ray but when it shows her trying to pick him up it is gone.
When Lucy is in the cake shop and smashes up the cup cakes she never gets any of the frosting on her hands. This occurs even when she smears it over Gru's face.
When Gru and Lucy break into Salsa and Salsa, the booby trap line is not visible before nor after Gru trips it.
When Gru is trying to enter El Macho's secret lair, a blob of guacamole falls on one of the tiles, causing an ax to fall. The guacamole disappears for the remainder of the scene.
Early in the movie one of the minions is vacuuming and answers the front door. The door opens in. Later when Gru is sitting on the front porch and Agnes comes out with an umbrella, the door opens out.
After Lucy shouts to the flight attendant "I choose Gru" she pushes the airplane door out. All planes that carry passengers for commercial flights have to be pulled inwards to open them. This is so they don't pop open if air pressure is suddenly lost and the plane cannot land immediately.
Margo and Antonio enter Salsa Salsa with the doors opening outwards. All other scenes show the doors opening inwards.
When one of El Macho's minions attacks Agnes and Margo and Agnes scream, it cracks pictures, a statue, and the minion's goggles, yet it does not crack Margo's glasses.
At the start of the film, when the secret lab is stolen from the Arctic, the footage is being viewed in AVL headquarters. The information on their screen includes latitude and longitude co-ordinates.
These co-ordinates are actually in southern Sudan, rather than the Arctic circle.
These co-ordinates are actually in southern Sudan, rather than the Arctic circle.
When Dave comes out of the kitchen in the cupcake shop with the minion cupcake, Lucy splits it apart, then pins Dave down and asks Gru if he knows him. But when she captured Gru and took him to the submarine, there were two minions with him and Lucy never asked about them then.
When Gru pops out of Lucy's trunk at the AVL he has a starfish on his head, this would imply 1) that there was a large enough gap in the trunk for the starfish to get in and therefore Gru to escape and 2) that water was getting into the trunk, and Gru would have drowned in the time it took to get to the AVL.
Eduardo's pet chicken, Pollito, is being referred to by male pronouns, even though "he" is clearly a "she", as he lacks the big tail of roosters.
2 minions survive and do not become purple minions. The purple minions wear black. At the end when El Macho is tasered, 4 minions instead of 2 are wearing blue.