The setting of Transylvania, Pennsylvania is similar to Ursolvania, Nepal from To All the Ghouls I've Loved Before (1985).
There are references to Son of Frankenstein (1939). Velma's ancestor is named Basil, a reference to Basil Rathbone, who portrayed Baron Wolf von Frankenstein. Inspector Krunch's and his fake hand refers to Inspector Krogh, portrayed by Lionel Atwill, who also had his hand replaced.
This is the first time since Scooby-Doo and the Samurai Sword (2008) that a chase song with lyrics has been used.
Mel Brooks' Young Frankenstein (1974) appears to be an influence. Elements include "Transyvanians" speaking German, Miss Vanders who resembles Frau Blucher, a barrel of root beer labeled Schwanstuker's, and Iago pronouncing his name incorrectly as "eye-ago."