INTERFACE SPECIFICATION

CELL-DYN® 1800 SYSTEM

LIST NO. 07H79-01

REVISION A

ABBOTT DIAGNOSTICS DIVISION

Abbott Laboratories
Abbott Park, IL 60064, USA
 INTERFACE SPECIFICATION
CELL-DYN® 1800 SYSTEM

Abbott Laboratories ©2003 Abbott Diagnostics Division

Abbott Park, IL 60064, USA 07H79-01A—February 2003
NOTES
 TABLE OF CONTENTS

1.0 Introduction..................................................................................................................... 1
2.0 Mechanical Interface ...................................................................................................... 1
3.0 Electrical Interface .......................................................................................................... 2
4.0 Data Interface ................................................................................................................. 2
5.0 Communication Protocol ................................................................................................ 3
6.0 Identification (ID) Segment ............................................................................................. 5
7.0 Results Segment ............................................................................................................ 8

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page i
 NOTES

Interface Specification CELL-DYN® 1800 System

Page ii 07H79-01—February 2003
1.0 Introduction
1.1 Purpose
This document describes the interfacing characteristics of the Abbott
CELL-DYN® 1800 automated hematology analyzer when attached to a Host.
1.2 Definitions and Conventions
Host: external computer or data collection system.
CELL-DYN 1800: Abbott CELL-DYN 1800.
This specification follows guidelines adopted at the Biomedical Instrumentation
Interface Standards Conference held at the University of Florida in December 1980
and at the University of Texas in Dallas, April 1981.
Signal designators and related characteristics follow EIA Standard RS232C as
summarized in “The Handbook of Computers and Computing,” Seidman and Flores,
Van Nostrad Reinhold, 1984; and “Integrated Circuits Applications Handbook,”
Arthur H. Seidman, John Wiley & Sons, 1983.
Numeric equivalents of characters are shown as hexadecimal (hex) values.

2.0 Mechanical Interface

2.1 Connector arrangement
The CELL-DYN 1800 provides a standard DB-9 male connector, labeled COM1,
mounted on the side of the instrument.
Pins on the DB-9 connector: (standard RS-232 9 pin assignments)
Pin 1: Data Carrier Detect (DCD input) (monitored)

Pin 2: Data to CELL-DYN 1800 (RD-receive data) (ACK/NAK, XON/XOFF)

Pin 3: Data to Host (LIS, results) (TD—transmit data)

Pin 4: Data Terminal Ready (DTR) (set true)

Pin 5: Signal Ground

Pin 6: Data Set Ready (DSR) (monitored)

Pin 7: Request to Send (RTS output) (set true)

Pin 8: Clear to Send (CTS input) (monitored, if requested in Setup)

Pin 9: Ring Indicate (RI input) (unused)

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3.0 Electrical Interface
3.1 Voltage levels and electrical characteristics are as defined by the EIA
RS-232C specification.
3.2 The maximum recommended cable length is 30 meters, or 100 feet. The actual
maximum workable cable length is dependent on the environment of the site, the
selected baud rate, and the equipment being connected together.

4.0 Data Interface

The system can be configured for data transmission using the COMPUTER SETUP Menu.
The configurable transmission parameters include data bits, stop bits, parity and baud rate.
4.1 The asynchronous method of data transmission (serial by bit) is used.
4.2 All information transmitted is in character form and is represented by 7-bit or ASCII.
4.3 Transmitted characters consist of one (1) start bit, seven (7) or eight (8) data bits
(least significant first), one or no parity bit, and one (1) or two (2) stop bits.
4.4 Parity may be selected as none, odd, or even.
4.5 The transmission speed may be selected from 1200, 2400, 4800, or 9600 bits per
second (bps).
4.6 With the exception of the control characters mentioned in Section 5.2, only printable
ASCII characters (hex 20 to hex 7E) are used in a message.

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5.0 Communication Protocol
5.1 Communication Modes
5.1.1 RUN Menu Automatic Transmit Mode
The User may select the Automatic Transmit mode on the COMPUTER
SETUP Menu. This mode allows the automatic transmission of results during
the RUN cycle. If Automatic Transmit mode has been selected, the user may
also choose whether or not to transmit histograms along with the count data.
5.1.2 DATALOG Menu Transmit Mode
On the DATALOG Menu, the user may select results from a single sample or
from multiple samples for transmission. Only count data may be transmitted
from the Data Log menu.
5.1.3 DISPLAY SPECIMEN Menu Transmit Mode
On the DISPLAY SPECIMEN Menu, the user may request transmission of
results from the specimen being displayed. Count data are transmitted.
Histograms are also transmitted if the Automatic Transmit mode is selected in
the COMPUTER SETUP Menu.
5.2 Transmission Control
Transmission control is provided in two ways: 1) XOFF/XON protocol in which the
Host transmits an XOFF character (hex 13) to stop transmission from the System and
an XON character (hex 11) to re-start transmission; and 2) CTS (Clear To Send)
hardware control.
The XOFF/XON protocol has a 1.5 second time-out. If no XON is received for 1.5
seconds after an XOFF, then transmission resumes anyway.
The CTS hardware control has no time-out. That is, no data transmission will be
started with CTS false. However, the transmission will still time-out if at any one time
CTS remains false for longer than the time-out selected in the COMPUTER SETUP
Menu (see Section 5.3.1 below). This is a different use and meaning for time-out, but
it is needed to prevent the Host from indefinitely delaying the CELL-DYN 1800 from
processing the next sample. Re-transmission requests are also supported and
discussed in Section 5.3.

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 5.3 Response from Host
5.3.1 Between-Transmission Time-out
The time-out interval after transmission of one message is programmable in
the COMPUTER SETUP Menu from 100 milliseconds to 9.9 seconds in 100
millisecond increments.
NOTE: The timer starts just as the ETX, which ends the message, is
transmitted.
5.3.2 Release for Next Message
The Host can release the CELL-DYN 1800 to send the next message by
sending an ACK (06 hex) before the time-out interval passes. Otherwise, the
CELL-DYN 1800 will begin transmission of the next message at the end of
the time-out interval in 5.3.1 above.
5.3.3 Re-transmission
If for any reason the Host requires re-transmission of the message, it signals
the CELL-DYN 1800 by sending a NAK (15 hex) before expiration of the
time-out. A message will be sent by the CELL-DYN 1800 a maximum of
three (3) times. After that, the same conditions prevail as after a time-out.
The Host may (it is desirable but not mandatory) acknowledge the re-
transmission with ACK or NAK. If ACK is sent, the CELL-DYN 1800 will
immediately start processing the next record (if any). If NAK is sent, the
record will NOT be transmitted a third time. Receipt of either the ACK or
NAK will allow the CELL-DYN 1800 to move to the next record more quickly.
5.4 Message Format
The message format consists of the following elements:
STX, ID SEGMENT, RESULT SEGMENT, CHECK SUM, ETX.
5.4.1 There are four (4) types of messages, each distinguished by its ID segment:
WBC Histogram Message
RBC Histogram Message
PLT Histogram Message
Count Data Message
5.4.2 The four messages taken together represent all the results of testing a single
specimen.
5.4.3 Each message begins with STX (hex 02).
5.4.4 The Identification (ID) segment is described fully in Section 6.
5.4.5 The Result segment is described fully in Section 7.
5.4.6 The Check Sum is always provided and may be optionally processed by the
Host to verify correct transmission. It is generated by taking the modulo-256
sum of all the characters in the message except the STX and ETX
characters. The two-digit hexadecimal representation of the Check Sum byte
is placed immediately before the ETX as two ASCII characters.
5.4.7 The ETX character (hex 03) is the last character of the message.

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 5.4.8 Message Length
Each of the three Histogram messages is 1204 characters long. The Count
Data message is 406 characters long. These message lengths count all
characters from STX to ETX inclusive.
5.4.9 Data Representation
Numeric data are transmitted in fields of fixed length with zeros used to fill empty
spaces on the left. Out-of-range numeric values are represented by strings of
">" characters (hex 3E), and undefined numeric values are represented by
strings of "–" characters (hex 2D). Alphanumeric data are transmitted in fields
of fixed length enclosed in double quotation marks (hex 22). Within the
quotation marks, the data are right-justified and blanks (hex 20) are used to fill
empty spaces. Fields are separated by commas (hex 2C).

6.0 Identification (ID) Segment

6.0.1 For counting purposes, the STX mentioned in Section 5.4.3 is taken as byte 1.
6.1 The Identification segment of each message identifies the type of message and the
specimen the message represents.
6.2 Message Type—Field 1
The Message Type field identifies the message. There are four message types:
WBC Histogram Message— Type "WBC"
RBC Histogram Message— Type "RBC"
PLT Histogram Message— Type "PLT"
Count Data Message— Type "__ _" (3 blanks)

6.3 Instrument Type—Field 2

The Instrument Type is an alphanumeric field of seven (7) characters enclosed in
double quotation marks. The string for the CELL-DYN 1800 is sent as
" CD1800".

6.4 Serial Number—Field 3

The Instrument Serial Number is an alphanumeric field of twelve (12) characters
enclosed in double quotation marks. It is not available in this release, so the field is
transmitted as "– – – – – – – – – – – –" (twelve dashes).

6.5 Sequence Number—Field 4

The Sequence Number is a numeric field of four (4) characters with a value ranging
from 0 to 9999.

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 6.6 Specimen Type—Field 5
The Specimen Type is the fifth field in the Identification Segment. It occupies a
numeric field of two characters whose value identifies the specimen type as follows:
00: Patient type "PATIENT"
01: Replicate 1 "REPLIC 1"
02: Replicate 2 "REPLIC 2"
03: Replicate 3 "REPLIC 3"
04: Replicate 4 "REPLIC 4"
05: Replicate 5 "REPLIC 5"
06: Replicate 6 "REPLIC 6"
07: Replicate 7 "REPLIC 7"
08: Replicate 8 "REPLIC 8"
09: Replicate 9 "REPLIC 9"
10: Low Control "LOW CTRL"
11: Normal Control "NOR CTRL"
12: High Control "HI CTRL"
13: Background "BACKGRD"
14: Electrical Background "ELEC BKGD"
15: Calibrator "CALIBRATR"
16: Gain Adjustment "GAIN ADJ"
17: Auto Calibration "AUTO CAL"

6.7 Operator ID—Field 6

The Operator ID is a alphanumeric field of three (3) digits enclosed in double

quotation marks. If no Operator ID is specified, the field is transmitted as "– – –".

6.8 Specimen Date—Field 7

The Specimen Date, giving the date on which the specimen was run, is an
alphanumeric field of eight (8) characters enclosed in double quotation marks. The
default format of the date is MM/DD/YY, where MM represents the month in two
digits, DD represents the day of the month, and YY represents the year. The date
format can be changed in the DATE/TIME Menu.

6.9 Specimen Time—Field 8

The Specimen Time is a alphanumeric field of five (5) characters enclosed in double
quotation marks. It gives the time at which the specimen was run in standard 24-
hour format.

6.10 Specimen ID—Field 9

The Specimen ID is an alphanumeric field of sixteen (16) characters enclosed in
double quotation marks. If the specimen is a patient specimen, the Specimen ID is
entered by the operator. Otherwise, the Specimen ID is generated by the
CELL-DYN 1800 to identify special types of samples, such as controls. If not
specified, the Specimen ID is transmitted as "– – – – – – – – – – – – – – – – "
(16 dashes). See Section 6.6 (Field 5).

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 6.11 Specimen Name—Field 10
The Specimen Name is the tenth field in the Identification Segment. It occupies a
text field of sixteen (16) characters enclosed in double quotation marks, and it is
undefined except for patient samples.

6.12 Specimen Sex—Field 11

The Specimen Sex field consists of one (1) character “M” or “F” enclosed in double
quotation marks. If not entered by the operator, Specimen Sex is transmitted as a
blank " ".
6.13 Specimen Date of Birth (DOB)—Field 12
The Specimen Date of Birth field has an identical format to Field 7, except that Field
7 is automatically generated by the CELL-DYN 1800. If DOB is not entered by the
operator, it is transmitted as "bb/bb/bb" where the “bb”s are actually 2 ASCII blanks
each.

6.14 Dr Name—Field 13
The Dr Name field is an alphanumeric field of twenty two (22) characters enclosed in
double quotation marks.

6.15 Collection Date—Field 14

The Collection Date field is an alphanumeric field of five (5) characters enclosed in
double quotation marks. The format is MM/DD, where MM represents the month in
two digits and DD represents the day. The year is not included. It gives the date at
which the specimen was collected. If the Collection Date is not entered, it is
transmitted as "bb/bb" where the “bb”s are 2 ASCII blanks as in Field 12 above.

6.16 Collection Time—Field 15

The Collection Time field is an alphanumeric field of five (5) characters enclosed in
double quotation marks. It gives the time at which the specimen was collected. If
the Collection Time is not entered, it is transmitted as "bb:bb" (“bb”s are 2 ASCII
blanks each and “:” is the middle byte).
6.17 Comment—Field 16
The Comment field is an alphanumeric field of sixteen (16) characters enclosed in
double quotation marks.
NOTE: Fields 1 to 16 above are common in both format AND actual content for a
given sample and, as such, are transmitted multiple times when histogram
transmission is requested (first—if sent—with all three histograms and then again
with the count record). Given today’s typical transmission speeds and the lengths of
the rest of the fields, the convenience of having the header information combined
with each data group has been chosen over the absolute smallest or fastest
transmission. Also, in the time frame of even normal sample processing, the
transmissions (at 9600 baud) should take less than 4 seconds out of the 60 second
(nominal) cycle time.

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7.0 Results Segment
7.1 The results for each specimen are sent in the format described. All numeric fields
are integers, and some need to be scaled by the Host.
7.2 Histogram Messages
Refer to Table 1, Histogram Messages, and Table 2, Histogram Record Dump.
7.2.1 Scale Factor—Field 17
The Scale Factor is a numeric field of five (5) characters. It is not
implemented at this time and is therefore always transmitted as 00000 (5
ASCII zeros without the surrounding double quotes).
7.2.2 Channel Data—Fields 18 through 273
The Channel Data fields are numeric fields of three (3) characters each,
giving normalized counts for every channel of the designated histogram
(WBC, RBC, or PLT). Data for each channel will occupy 4 bytes total since
there will be a comma between each 3-digit value. Each value is right-
justified with ASCII zero “0” (not null) characters to keep the fields a fixed
length. So a channel value of binary zero is transmitted as three ASCII zero
characters (000).
NOTE: A comma (,) follows the last field which immediately precedes the
CRC characters.

7.3 Count Data Message

Refer to Table 3, Count Data Messages, and Table 4, Count Record Dump.
The parameters reported by the instrument may be represented in any of four
different sets of measurement units as follows:
Set 1—Standard USA
Set 2—SI
Set 3—Modified SI (HGB/MCHC in mmol/L, MCH in fmol)
Set 4—Modified SI (HCT/PCT in %)
NOTE: The numeric values transmitted to the host are always sent in Standard USA
units even if the unit of measure, also being transmitted, is Set 2, 3, or 4.

To convert the integer transmitted in the field corresponding to a particular

parameter to the correct value for that parameter in the units being used, the
decimal point must be moved leftward from its implied position to the right of the
integer. The description of each field gives a shift count indicating how many places
to move the decimal point in each case. If, for example, the decimal point is to be
moved two places to the left, a field value of 00123 becomes 1.23. An integer
representing the Units Set currently in effect is transmitted in the Units Set field
(Section 7.3.46).
For Units Set 3, the HGB, MCH, and MCHC values must be multiplied by 0.6206
after the decimal point has been positioned.
The units of measure associated with the four sets are explained in the
CELL-DYN 1800 Operator’s Manual.

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 7.3.1 WBC Count—Field 17
The WBC Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 K/µL
2–3 1 G/L
4 1 10E9/L

7.3.2 Spare Field—Field 18

The Spare Field is a numeric field of five (5) characters. It has a constant value
of zero (0) at this time, but is transmitted as five “-“ (dashes) without surrounding
double quotes, since it is a numeric field.

7.3.3 LYM Count—Field 19

The LYM Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 K/µL
2–3 1 G/L
4 1 10E9/L

7.3.4 MID Count—Field 20

The MID Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 K/µL
2–3 1 G/L
4 1 10E9/L

7.3.5 GRAN Count—Field 21

The GRAN Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 K/µL
2–3 1 G/L
4 1 10E9/L

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 7.3.6 Spare Field—Field 22
The Spare Field is a numeric field of five (5) characters. It has a constant value
of zero (0) at this time, but is transmitted as five “-“ (dashes) without surrounding
double quotes, since it is a numeric field.

7.3.7 RBC Count—Field 23

The RBC Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 2 M/µL
2–3 2 T/L
4 2 10E12/L

7.3.8 HGB Value—Field 24

The HGB Value is in a numeric field, five (5) characters long.
Units Set Shift Count Units Label
1 1 g/dL
2 0 g/L
3 1 (x 0.6206) mmol/L
4 0 g/L

7.3.9 HCT Value—Field 25

The HCT Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 %
2–3 3 L/L
4 1 %

7.3.10 MCV Value—Field 26

The MCV Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–4 1 fL

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 7.3.11 MCH Value—Field 27
The MCH Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1-2 1 pg
3 2 (x 0.6206) fmol
4 1 pg

7.3.12 MCHC Value—Field 28

The MCHC Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 1 g/dL
2 0 g/L
3 1 (x 0.6206) mmol/L
4 0 g/L

7.3.13 RDW Value—Field 29

The RDW Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–4 1 %

7.3.14 PLT Count—Field 30

The PLT Count is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 0 K/µL
2–3 0 G/L
4 0 10E9/L

7.3.15 MPV Value—Field 31

The MPV Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–4 1 fL

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 7.3.16 PCT Value*—Field 32
The PCT Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1 2 %
2–3 1 mL/L
4 2 %

7.3.17 PDW Value*—Field 33

The PDW Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–4 1 10(GSD)

* Clinical significance has not been established for these parameters; therefore
they are not reportable in the U.S.

7.3.18 Spare Field—Field 34

The Spare Field is a numeric field of five (5) characters. It has a constant value
of zero (0) at this time, but is transmitted as five “-“ (dashes) without surrounding
double quotes, since it is a numeric field.

7.3.19 LYM % Value—Field 35

The LYM % Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–4 1 %

7.3.20 MID % Value—Field 36

The MID % Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–4 1 %

7.3.21 GRAN % Value—Field 37

The GRAN % Value is in a numeric field five (5) characters long.
Units Set Shift Count Units Label
1–4 1 %

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 7.3.22 Spare Field—Field 38
The Spare Field is a numeric field of five (5) characters. It has a constant value of
zero (0) at this time, but is transmitted as five “-“ (dashes) without the surrounding
double quotes, since it is a numeric field.

7.3.23 Spare Field—Field 39

The Spare Field is in a numeric field. It has not been implemented at this time, so it
is always transmitted as a “-“ single dash without the surrounding double quotes.

7.3.24 Spare Flag—Field 40

The Spare Flag is a numeric field of one (1) character. It has a constant value of
zero (0) at this time, but is transmitted as a single “-“ (dash) without the surround
double quotes, since is presumed to be a spare numeric flag.

7.3.25 R4 WBC Flag—Field 41

The R4 WBC Flag is in a numeric field of one character. A value of 1 indicates
that the flag is set, and a value of 0 indicates that it is clear.

7.3.26 GR3 WBC Flag—Field 42

The GR3 WBC Flag is in a numeric field of one character. A value of 1 indicates
that the GRAN R3 flag is set, and a value of 0 indicates that it is clear.

7.3.27 MR3 WBC Flag—Field 43

The MR3 WBC Flag is in a numeric field of one character. A value of 1 indicates
that the Mono R3 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.28 MR2 WBC Flag—Field 44

The MR2 WBC Flag is in a numeric field of one character. A value of 1 indicates
that the Mono R2 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.29 LR2 WBC Flag—Field 45

The LR2 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the Lym R2 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.30 R1 WBC Flag—Field 46

The R1 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the R1 WBC flag is set, and a value of 0 indicates that it is clear.

7.3.31 R0 WBC Flag—Field 47

The R0 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the flag is set, and a value of 0 indicates that it is clear.

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 7.3.32 Spare Flags—Fields 48 through 60
There are thirteen Spare Flag fields which are each a numeric field of one (1)
character. It has a constant value of zero (0) at this time. These are transmitted
as follows (no surrounding quotes): –,–,–,–,–,–,–,–,–,–,–,–,–,

7.3.33 LRI Flag—Field 61

The LRI Flag is in a numeric field of two characters. A value of 16 indicates that
the flag is set, and a value of 00 indicates that it is clear.

7.3.34 URI Flag—Field 62

The URI Flag is in a numeric field of two characters. A value of 32 indicates that
the flag is set, and a value of 00 indicates that it is clear.

7.3.35 WBC Lower Meniscus Time—Field 63

The WBC Lower Meniscus Time is in a numeric field five (5) characters long. It
gives the time in units of milliseconds.

7.3.36 WBC Upper Meniscus Time—Field 64

The WBC Upper Meniscus Time is in a numeric field five (5) characters long. It
gives the time in units of milliseconds.

7.3.37 RBC Lower Meniscus Time—Field 65

The RBC Lower Meniscus Time is in a numeric field five (5) characters long. It
gives the time in units of milliseconds.

7.3.38 RBC Upper Meniscus Time—Field 66

The RBC Upper Meniscus Time is in a numeric field five (5) characters long. It
gives the time in units of milliseconds.

7.3.39 Recount RBC Lower Meniscus Time—Field 67

The Recount RBC Lower Meniscus Time is in a numeric field five (5) characters
long. It gives the time in units of milliseconds. It has a value of 0 if there was no
recount.

7.3.40 Recount RBC Upper Meniscus Time—Field 68

The Recount RBC Upper Meniscus Time is in a numeric field five (5) characters
long. It gives the time in units of milliseconds. It has a value of 0 if there was no
recount.

7.3.41 Limits Set—Field 69

The Limits Set is a numeric field of one character. It indicates which set of
patient limits (1 to 4) was in effect for the given patient specimen.

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 7.3.42 Sample Mode—Field 70
The Sample Mode is an alphanumeric field of one character. An “O” (letter O)
indicates that the specimen was taken in Open Sample mode; a “P” indicates Pre-
Dilute mode.

7.3.43 RBC Metering Fault Flag—Field 71

The RBC Metering Fault flag is a numeric field of one character. A “0” (zero)
indicates that there was no metering fault. A “1” (one) indicates a flow error, and a
“2” (two) indicates a clog.

7.3.44 WBC Metering Fault Flag—Field 72

The WBC Metering Fault flag is a numeric field of one character. A “0” (zero)
indicates that there was no metering fault. A “1” (one) indicates a flow error, and a
“2” (two) indicates a clog.

7.3.45 Sampling Error or Other Sample Processing Fault— Field 73

This is a numeric field of one character. A “0” (zero) indicates there was no error. A
“1” (one) indicates there was an error.

7.3.46 Units Set Field—Field 74

This is a numeric field of one character. The value is 1, 2, 3, or 4 as described in
Section 7.3.

There are four sets of measurement units. A problem arises under the following conditions:

1. Some data is collected and stored under one unit of measure.

2. Subsequently, data is collected and stored under another unit of measure.
3. All the data is transmitted to a Laboratory Information System.

The numeric results data, starting in field 17, are always sent as Standard USA units even
though that data may be displayed in a different unit of measure on the instrument. The
displayed unit of measure, sent in field 74, is transmitted for informational purposes only and
may or may not correspond to the Standard USA format of the transmitted numeric results data.

NOTE: A comma (,) follows the last field which immediately precedes the CRC characters.

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 TABLE 1 HISTOGRAM MESSAGES
Start at Byte
Field # Description Byte Length Paragraph #
N/A STX (0x02) 1 1 5.4.3
1 Message Type 2 3* 6.2
2 Instrument Type 8 7* 6.3
3 Serial Number 18 12* 6.4
4 Sequence Number 33 4 6.5
5 Specimen Type 38 2 6.6
6 Operator ID 41 3* 6.7
7 Specimen Date 47 8* 6.8
8 Specimen Time 58 5* 6.9
9 Specimen ID 66 16* 6.10
10 Specimen Name 85 16* 6.11
11 Specimen Sex 104 1* 6.12
12 Specimen DOB 108 8* 6.13
13 Dr Name 119 22* 6.14
14 Collect Date 144 5* 6.15
15 Collect Time 152 5* 6.16
16 Comment 160 16* 6.17
The above fields are common
to both this Table and Table 3
17 Scale Factor (n/a) 179 5 7.2.1
18 Channel 1 Data 185 3 7.2.2
" " " " " ...
273 Channel 256 Data 1205 3 7.2.2
N/A CRC (2 hex ASCII printable) 1209 2 5.4.6
N/A ETX (0x03) 1211 1 5.4.7

*indicates characters enclosed in double quotation marks.

Note: If a field contains 3 characters plus double quotation marks and a comma, the
total number of bytes used is 6. See the Histogram Message Example.

Interface Specification CELL-DYN® 1800 System

Page 16 of 36 07H79-01—February 2003
 TABLE 2 HISTOGRAM RECORD DUMP

02 22 57 42 43 22 2C 22 20 43 44 31 37 30 30 22 . "WBC", "CD1800"
2C 22 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 22 2C , "– – – – – – – – – – – –",
34 32 38 38 2C 30 30 2C 22 2D 2D 2D 22 2C 22 31 4288,00, "– – –","1
30 2F 31 39 2F 39 34 22 2C 22 31 36 3A 34 34 22 0/19/94", "16:44"
2C 22 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D , "– – – – – – – – – – – – – – – –" , “
2D 2D 22 2C 22 20 20

20 20 20 20 20 20 20 20 20 20 20 20 20 31 22 2C 1",
22 4D 22 2C 22 20 20 2F 20 20 2F 20 20 22 2C 22 "M" , " / / " , "
20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20

20 20 20 20 20 20 22 2C 22 20 20 2F 20 20 22 2C " , " / ",

22 20 20 3A 20 22 22 C 22 20 20 20 20 20 20 20 " :","
20 20 20 20 20 20 20 20 20 22 2C 30 30 30 30 30 ",00000
2C 30 30 30 2C 30 30 30 2C 30 30 30 2C 30 30 30 ,000,000,000,000
2C 30 30 30 2C 30 30 30 2C 30 30 30 2C 30 30 30 ,000,000,000,000
2C 30 30 30 2C 30 34 32 2C 30 34 38 2C 30 35 34 ,000,042,048,054
2C 30 35 37 2C 30 35 39 2C 30 35 35 2C 30 34 35 ,057,059,055,045
2C 30 33 35 2C 30 32 35 2C 30 32 30 2C 30 31 36 ,035,025,020,016
...
... (channels have been deleted)
...
2C 30 35 32 2C 30 35 33-2C 30 35 31 2C 30 35 30 ,052,053,051,050
2C 30 34 39 2C 30 34 38-2C 30 34 37 2C 30 30 30 ,049,048,047,000
2C 30 30 30 2C 30 30 30-2C 30 30 30 2C 30 30 30 ,000,000,000,000
2C 36 35 03 ,65.

Note: Each line contains 16 characters starting with 1, not zero. The “02” and “.” bolded on the
first line refer to the STX character. The “03” and “65” bolded on the last line refer to the
ETX and CRC characters respectively.

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 17 of 36
 TABLE 3 COUNT DATA MESSAGES

Field # Description Start at Byte Paragraph #

n/a STX (0x02) 1 1 5.4.3
1 Message Type 2 3* 6.2
2 Instrument Type 8 7* 6.3
3 Serial Number 18 12* 6.4
4 Sequence Number 33 4 6.5
5 Specimen Type 38 2 6.6
6 Operator ID 41 3* 6.7
7 Specimen Date 47 8* 6.8
8 Specimen Time 58 5* 6.9
9 Specimen ID 66 16* 6.10
10 Specimen Name 85 16* 6.11
11 Specimen Sex 104 1* 6.12
12 Specimen DOB 108 8* 6.13
13 Dr Name 119 22* 6.14
14 Collect Date 144 5* 6.15
15 Collect Time 152 5* 6.16
16 Comment 160 16* 6.17
The above fields are common
to both this Table and Table 1
17 WBC Count 179 5 7.3.1
18 Spare Field 185 5 7.3.2
19 LYM Count 191 5 7.3.3
20 MID Count 197 5 7.3.4
21 GRAN Count 203 5 7.3.5
22 Spare Field 209 5 7.3.6
23 RBC Count 215 5 7.3.7
24 HGB Value 221 5 7.3.8
25 HCT Value 227 5 7.3.9
26 MCV Value 233 5 7.3.10
27 MCH Value 239 5 7.3.11
28 MCHC Value 245 5 7.3.12
29 RDW Value 251 5 7.3.13
30 PLT Count 257 5 7.3.14
31 MPV Value 263 5 7.3.15
32 PCT Value 269 5 7.3.16
33 PDW Value 275 5 7.3.17
34 Spare Field 281 5 7.3.18
35 LYM % Value 287 5 7.3.19
36 MID % Value 293 5 7.3.20
37 GRAN % Value 299 5 7.3.21
38 Spare Field 305 5 7.3.22
39 Spare Field 311 1 7.3.23

Interface Specification CELL-DYN® 1800 System

Page 18 of 36 07H79-01—February 2003
 TABLE 3 COUNT DATA MESSAGES
(Continued)

40 Spare Field 313 1 7.3.24

41 R4 WBC Flag 315 1 7.3.25
42 GR3 WBC Flag 317 1 7.3.26
43 MR3 WBC Flag 319 1 7.3.27
44 MR2 WBC Flag 321 1 7.3.28
45 LR2 WBC Flag 323 1 7.3.29
46 R1 WBC Flag 325 1 7.3.30
47 R0 WBC Flag 327 1 7.3.31
48—60 Spare Flags 329 1 7.3.32
61 LRI Flag 355 2 7.3.33
62 URI Flag 358 2 7.3.34
63 WBC Lower Meniscus Time 361 5 7.3.35
64 WBC Upper Meniscus Time 367 5 7.3.36
65 RBC Lower Meniscus Time 373 5 7.3.37
66 RBC Upper Meniscus Time 379 5 7.3.38
Recount RBC Lower Meniscus
67 385 5 7.3.39
Time
Recount RBC Upper Meniscus
68 391 5 7.3.40
Time
69 Limits Set 397 1 7.3.41
70 Sample Mode 399 1* 7.3.42
71 RBC Metering Fault Flag 403 1 7.3.43
72 WBC Metering Fault Flag 405 1 7.3.44
Sampling Error/Incomplete
73 407 1 7.3.45
Aspiration Flag
74 Units Set Field 409 1 7.3.46
n/a CRC (2 hex ASCII printable) 411 2 5.4.6
n/a ETX (0x03) 413 1 5.4.7

* indicates characters enclosed in double quotation marks

Note: If a field contains 3 characters plus double quotation marks and a comma, the total
number of bytes used is 6. See the Count Data Message Example.

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 19 of 36
 TABLE 4 COUNT RECORD DUMP

02 22 20 20 20 22 2C 22-20 43 44 31 37 30 30 22 . " " , " CD1800"

2C 22 2D 2D 2D 2D 2D 2D-2D 2D 2D 2D 2D 2D 22 2C , "– – – – – – – – – – – –",
34 32 38 38 2C 30 30 2C-22 2D 2D 2D 22 2C 22 31 4288,00, "– – –","1
30 2F 31 39 2F 39 34 22-2C 22 31 36 3A 34 34 22 0/19/94", "16:44"
2C 22 2D 2D 2D 2D 2D 2D-2D 2D 2D 2D 2D 2D 2D 2D , "– – – – – – – – – – – – – – – –" , "
2D 2D 22 2C 22 20 20
20 20 20 20 20 20 20 20-20 20 20 20 20 31 22 2C 1",
22 4D 22 2C 22 20 20 2F-20 20 2F 20 20 22 2C 22 "M" , " / / ","
20 20 20 20 20 20 20 20-20 20 20 20 20 20 20 20
20 20 20 20 20 20 22 2C-22 20 20 2F 20 20 22 2C "," / ",
22 20 20 3A 20 20 22 2C-22 20 20 20 20 20 20 20 " :","
20 20 20 20 20 20 20 20-20 22 2C 30 30 35 32 35 ",00525
2C 2D 2D 2D 2D 2D 2C 30-30 30 31 30 2C 30 30 30 ,– – – – –,00010,000
31 36 2C 30 30 34 39 39-2C 2D 2D 2D 2D 2D 2C 30 16,00499,– – – – –,0
30 32 35 31 2C 30 30 30-37 34 2C 30 30 32 32 36 0251,00074,00226
2C 30 30 39 30 30 2C 30-30 32 39 35 2C 30 30 33 ,00900,00295,003
32 37 2C 30 30 31 38 37-2C 30 30 33 39 35 2C 30 27,00187,00395,0
30 30 36 38 2C 30 30 30-32 37 2C 30 30 31 38 38 0068,00027,00188
2C 2D 2D 2D 2D 2D 2C 30-30 30 31 39 2C 30 30 30 ,– – – – –,00019,000
33 31 2C 30 30 39 35 30-2C 2D 2D 2D 2D 2D 2C 2D 31,00950,– – – – –,–
2C 2D 2C 30 2C 30 2C 30-2C 31 2C 31 2C 30 2C 30 ,–,0,0,0,1,1,0,0
2C 2D 2C 2D 2C 2D 2C 2D-2C 2D 2C 2D 2C 2D 2C 2D ,–,–,–,–,–,–,–,–
2C 2D 2C 2D 2C 2D 2C 2D-2C 2D 2C 30 30 2C 30 30 ,–,–,–,–,–,00,00
2C 30 34 38 33 37 2C 30-32 31 32 31 2C 30 36 34 ,04837,02121,064
31 31 2C 30 35 30 37 30-2C 30 30 30 30 30 2C 30 11,05070,00000,0
30 30 30 30 2C 34 2C 22-4F 22 2C 30 2C 30 2C 30 0000,4, "O",0,0,0
2C 31 2C 33 37 03 ,1,37.

Interface Specification CELL-DYN® 1800 System

Page 20 of 36 07H79-01—February 2003
 HISTOGRAM MESSAGE EXAMPLE

Actual transmission [remarks for text here]

(no CR/LF)
STX [start of text]
"WBC", [message type] Field 1
" CD1800", [instrument type]
"– – – – – – – – – – – –", [serial #]
1040, [sequence no.]
00, [specimen type] Field 5
"123", [operator ID]

"08/31/03", [specimen date]

"12:34", [specimen time]

"123456789ABCDEFG", [specimen ID]

" JOHN DOE", [specimen name] Field 10

"M", [specimen sex]

"10/15/46", [specimen DOB]

" DR JANE DOE", [doctor name]

"08/30", [collect date]

"09:50", [collect time] Field 15

" sample 1", [comment] Field 16

00000, [scale factor n/a] Field 17

000, [channel 1 data] Field 18

000, [channel 2 data]

000, [channel 3 data]

000, [channel 4 data]

000, [channel 5 data]

000, [channel 6 data]

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 21 of 36
 HISTOGRAM MESSAGE EXAMPLE
(Continued)
000, [channel 7 data]

000, [channel 8 data]

000, [channel 9 data]

000, [channel 10 data]

000, [channel 11 data]

000, [channel 12 data]

000, [channel 13 data]

000, [channel 14 data]

000, [channel 15 data]

000, [channel 16 data]

000, [channel 17 data]

000, [channel 18 data]

000, [channel 19 data]

000, [channel 20 data]

000, [channel 21 data]

000, [channel 22 data]

000, [channel 23 data]

000, [channel 24 data]

000, [channel 25 data]

034, [channel 26 data]

034, [channel 27 data]

034, [channel 28 data]

034, [channel 29 data]

Interface Specification CELL-DYN® 1800 System

Page 22 of 36 07H79-01—February 2003
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

054, [channel 30 data]

054, [channel 31 data]

054, [channel 32 data]

054, [channel 33 data]

080, [channel 34 data]

080, [channel 35 data]

080, [channel 36 data]

080, [channel 37 data]

093, [channel 38 data]

093, [channel 39 data]

093, [channel 40 data]

093, [channel 41 data]

080, [channel 42 data]

080, [channel 43 data]

080, [channel 44 data]

080, [channel 45 data]

071, [channel 46 data]

071, [channel 47 data]

071, [channel 48 data]

071, [channel 49 data]

063, [channel 50 data]

063, [channel 51 data]

063, [channel 52 data]

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 23 of 36
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

063, [channel 53 data]

041, [channel 54 data]

041, [channel 55 data]

041, [channel 56 data]

041, [channel 57 data]

032, [channel 58 data]

041, [channel 59 data]

029, [channel 60 data]

029, [channel 61 data]

029, [channel 62 data]

029, [channel 63 data]

029, [channel 64 data]

027, [channel 65 data]

027, [channel 66 data]

027, [channel 67 data]

027, [channel 68 data]

027, [channel 69 data]

034, [channel 70 data]

034, [channel 71 data]

034, [channel 72 data]

034, [channel 73 data]

034, [channel 74 data]

039, [channel 75 data]

039, [channel 76 data]

Interface Specification CELL-DYN® 1800 System

Page 24 of 36 07H79-01—February 2003
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

039, [channel 77 data]

039, [channel 78 data]

037, [channel 79 data]

037, [channel 80 data]

037, [channel 81 data]

037, [channel 82 data]

037, [channel 83 data]

037, [channel 84 data]

037, [channel 85 data]

041, [channel 86 data]

041, [channel 87 data]

041, [channel 88 data]

041, [channel 89 data]

046, [channel 90 data]

046, [channel 91 data]

046, [channel 92 data]

046, [channel 93 data]

046, [channel 94 data]

046, [channel 95 data]

046, [channel 96 data]

046, [channel 97 data]

046, [channel 98 data]

046, [channel 99 data]

046, [channel 100 data]

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 25 of 36
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

046, [channel 101 data]

049, [channel 102 data]

046, [channel 103 data]

046, [channel 104 data]

046, [channel 105 data]

054, [channel 106 data]

054, [channel 107 data]

054, [channel 108 data]

054, [channel 109 data]

058, [channel 110 data]

054, [channel 111 data]

054, [channel 112 data]

054, [channel 113 data]

063, [channel 114 data]

063, [channel 115 data]

063, [channel 116 data]

063, [channel 117 data]

066, [channel 118 data]

063, [channel 119 data]

063, [channel 120 data]

063, [channel 121 data]

063, [channel 122 data]

066, [channel 123 data]

066, [channel 124 data]

Interface Specification CELL-DYN® 1800 System

Page 26 of 36 07H79-01—February 2003
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

066, [channel 125 data]

066, [channel 126 data]

066, [channel 127 data]

066, [channel 128 data]

066, [channel 129 data]

066, [channel 130 data]

068, [channel 131 data]

068, [channel 132 data]

068, [channel 133 data]

068, [channel 134 data]

073, [channel 135 data]

068, [channel 136 data]

068, [channel 137 data]

068, [channel 138 data]

068, [channel 139 data]

078, [channel 140 data]

080, [channel 141 data]

080, [channel 142 data]

080, [channel 143 data]

080, [channel 144 data]

073, [channel 145 data]

073, [channel 146 data]

073, [channel 147 data]

073, [channel 148 data]

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 27 of 36
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

073, [channel 149 data]

073, [channel 150 data]

075, [channel 151 data]

083, [channel 152 data]

083, [channel 153 data]

083, [channel 154 data]

083, [channel 155 data]

083, [channel 156 data]

083, [channel 157 data]

083, [channel 158 data]

083, [channel 159 data]

088, [channel 160 data]

090, [channel 161 data]

090, [channel 162 data]

090, [channel 163 data]

090, [channel 164 data]

090, [channel 165 data]

090, [channel 166 data]

090, [channel 167 data]

090, [channel 168 data]

090, [channel 169 data]

095, [channel 170 data]

095, [channel 171 data]

095, [channel 172 data]

Interface Specification CELL-DYN® 1800 System

Page 28 of 36 07H79-01—February 2003
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

095, [channel 173 data]

110, [channel 174 data]

095, [channel 175 data]

095, [channel 176 data]

095, [channel 177 data]

110, [channel 178 data]

110, [channel 179 data]

110, [channel 180 data]

110, [channel 181 data]

110, [channel 182 data]

105, [channel 183 data]

110, [channel 184 data]

110, [channel 185 data]

110, [channel 186 data]

100, [channel 187 data]

100, [channel 188 data]

100, [channel 189 data]

100, [channel 190 data]

100, [channel 191 data]

100, [channel 192 data]

093, [channel 193 data]

085, [channel 194 data]

085, [channel 195 data]

085, [channel 196 data]

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 29 of 36
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

085, [channel 197 data]

085, [channel 198 data]

078, [channel 199 data]

078, [channel 200 data]

085, [channel 201 data]

085, [channel 202 data]

085, [channel 203 data]

085, [channel 204 data]

085, [channel 205 data]

085, [channel 206 data]

085, [channel 207 data]

083, [channel 208 data]

083, [channel 209 data]

083, [channel 210 data]

083, [channel 211 data]

073, [channel 212 data]

083, [channel 213 data]

083, [channel 214 data]

083, [channel 215 data]

083, [channel 216 data]

083, [channel 217 data]

083, [channel 218 data]

083, [channel 219 data]

083, [channel 220 data]

Interface Specification CELL-DYN® 1800 System

Page 30 of 36 07H79-01—February 2003
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

066, [channel 221 data]

054, [channel 222 data]

054, [channel 223 data]

054, [channel 224 data]

054, [channel 225 data]

054, [channel 226 data]

054, [channel 227 data]

051, [channel 228 data]

051, [channel 229 data]

051, [channel 230 data]

051, [channel 231 data]

051, [channel 232 data]

051, [channel 233 data]

051, [channel 234 data]

046, [channel 235 data]

034, [channel 236 data]

034, [channel 237 data]

034, [channel 238 data]

034, [channel 239 data]

034, [channel 240 data]

034, [channel 241 data]

034, [channel 242 data]

034, [channel 243 data]

034, [channel 244 data]

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 31 of 36
 HISTOGRAM MESSAGE EXAMPLE
(Continued)

034, [channel 245 data]

034, [channel 246 data]

034, [channel 247 data]

027, [channel 248 data]

027, [channel 249 data]

027, [channel 250 data]

027, [channel 251 data]

027, [channel 252 data]

027, [channel 253 data]

000, [channel 254 data]

000, [channel 255 data]

000, [channel 256 data] Field 273

[note comma after

channel 256 but not
after the checksum]

E7 [checksum]
NOTE: Sample checksum value may
not match this record’s data.

ETX [end of text]

Interface Specification CELL-DYN® 1800 System

Page 32 of 36 07H79-01—February 2003
 COUNT DATA MESSAGE EXAMPLE

STX [start of text] Field 1

"WBC", [message type]

" CD1800", [instrument type]

"– – – – – – – – – – – –", [serial #]

1040, [sequence no.]

00, [specimen type] Field 5

"123", [operator ID]

"08/31/03", [specimen date]

"12:34", [specimen time]

"123456789ABCDEFG", [specimen ID]

" JOHN DOE", [specimen name] Field 10

"M", [specimen sex]

"10/15/46", [specimen DOB]

" DR JANE DOE", [doctor name]

"08/30", [collect date]

"09:50", [collect time] Field 15

" sample 1", [comment] Field 16

00083, [WBC count] Field 17

– – – – –, [spare field]

00015, [LYM count]

00006, [MID count] Field 20

00062, [GRAN count]

– – – – –, [spare field]

00476, [RBC count] Field 23

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 33 of 36
 COUNT DATA MESSAGE EXAMPLE
(continued)

00166, [HGB value] Field 24

00447, [HCT value]

00940, [MCV value]

00349, [MCH value]

00371, [MCHC value]

00118, [RDW value]

00230, [PLT value] Field 30

00084, [MPV value]

00019, [PCT value]

00174, [PDW value]

– – – – –, [spare field]

00178, [LYM% value] Field 35

00077, [MID% value]

00745, [GRAN% value]

– – – – –, [spare field]

–, [spare field]

–, [spare field] Field 40

0, [R4 WBC flag]

0, [GR3 WBC flag]

0, [MR3 WBC flag]

1, [MR2 WBC flag]

0, [LR2 WBC flag] Field 45

0, [R1 WBC flag]

1, [R0 WBC flag]

Interface Specification CELL-DYN® 1800 System

Page 34 of 36 07H79-01—February 2003
 COUNT DATA MESSAGE EXAMPLE
(continued)

–, [spare field] Field 48

–, [spare field] Field 49

–, [spare field] Field 50

–, [spare field]

–, [spare field]

–, [spare field]

–, [spare field]

–, [spare field] Field 55

–, [spare field]

–, [spare field]

–, [spare field]

–, [spare field]

–, [spare field] Field 60

00, [LRI flag]

00, [URI flag]

04862, [WBC lower meniscus time]

02363, [WBC upper meniscus time]

08107, [RBC lower meniscus time]

07656, [RBC upper meniscus time]

00000, [recount lower time]

21845, [recount upper time]

1, [limits set]

"O", [sample mode] Field 70

0, [RBC metering fault]

CELL-DYN® 1800 System Interface Specification

07H79-01—February 2003 Page 35 of 36
 COUNT DATA MESSAGE EXAMPLE

(continued)

0, [WBC metering fault]

0, [sample error/incomplete
aspiration] Field 73

1, [units set field] Field 74

9B [checksum]
NOTE: Sample checksum value
may not match this record’s data.

ETX [end of text]

Interface Specification CELL-DYN® 1800 System

Page 36 of 36 07H79-01—February 2003