1.
The medium used in membrane filter technique was
a. EMB agar b. EMR-Vp medium c. Lactose broth d. Endo agar
2. Lysol is a
a. Sterilent b. Disinfectant c. Antiseptic d. Antifungal agent
3. Which of the following is a neutral stain?
a. Picric acid b. Gmiemsa c. Neutral red d. Malachite green
4. Peptone water medium is an example for
a. Synthetic medium b. Semisynthetic medium c. Differential medium d. None of these
5. The method in which the cells are frozen dehydrated is called
a. Pasteurization b. Dessication c. Disinfection d. Lypophilization
6. The technique used to avoid all microorganisms is accomplished by
a. Sterlization b. Disinfection c. Surgical sterilization d. Disinfection Sterilization
7. Thermal death time is
a. Time required to kill all cells at a given temperature b. Temperature that kills all cells in a
given time c. Time and temperature needed to kill all cells d. All of the above
8. A culture medium the exact composition of which is not known was called as
a. Simple b. Complex c. Defined d. Natural
9. Elek’s gel diffusion test is used for the detection of
a. Tetani toxin b. Cholera toxin c. Diophtheria toxin d. Toxoid
10. Temperature required for pasteurization is
a. Above 150o C b. Below 100o C c. 110o C d. None of these
11. Separation of a single bacterial colony is calle
a. Isolation b. Separation c. Pure culturing d. All of these
12. Which of the following is ionizing radiation?
a. U.V. rays b. IR c. γ-rays d. None of these
13. Which of the following induces dimerisation of thymine?
a. X-rays b. U.V. rays c. ã-rays d. None of these
14. When food material are preserved at a temperature just above freezing temperature, the
process is called.
a. Freezing b. Pasteurisation c. Chilling d. Frosting
15. Which of the following method of sterilization has no effect on spores?
a. Drying b. Hot air oven c. Autoclave d. None of these
16. Treponema pallidum can be best indentified using
a. Fluorescence microscope b. Bright field microscope c. Dark field microscope d. Flourescence
microscope
17. Autoclaving is carried at
a. Dry heat b. Atmospheric pressure c. 120o C d. All of these
18. Temperature in pasteurization is
a. 62.8o C b. 35.7o C c. 68.2o C d. 60.8o C
19. The bacterial culture prepared by pure culture method is
a. Inoculum b. Suspension c. Dilution d. None of these
20. Algae are rich in
a. Carbohydrates b. Proteins c. Vitamins d. All of these
21. L-Lysine is produced from
a. Corynebacterium glutamicum b. Clostridium botulinum c. Mycobacterium sps d.
Pseudomonas
22. The orderly increase in the quantity of all of the cellular components is known as
a. Reproduction b. Growth c. Binary fission d. None of these
23. Theobacillus thio oxidans grow at pH
a. 7.0 b. 1.0 c. 6.0 d. 9.5
24. Slow freezing requires the conditions
a. 0o C to 15o C for 15 min. b. – 6 o C to – 10o C for 10 min. c. – 15o C to 3 to 72 hrs. d. None of
these
25. Discontinuous heating is called
a. Pasteurization b. Sterilization c. Fermentation d. Tindalisation
26. Isolation is
a. Purification of culture b. Introduction of inoculum c. Separation of a single colony d. To grow
microorganisms on surfaces
27. The condition required for autoclave
a. 121o C temp.and 15 lbs. pressure for 20 min. b. 120o C temp.and 20 lbs. pressure for 30 min
c. 150o C temp.for 1 hr. d. 130o C temp for 2 hr.
28. Lysozyme is effective against
a. Gram negative bacteria b. Gram positive bacteria c. Protozoa d. Helminthes
29. Blood agar medium is
a. Enrichment medium b. Enriched medium c. Selective medium d. Differential medium
30. Infrared radiation is a method of steril- ization by
a. Dry heat b. Moist heat c. Chemical method d. Mechanical method
31. Lyophilization means
a. Sterilization b. Freeze-drying c. Burning to ashes d. Exposure to formation
32. Temperature used for hot air oven is
a. 100o C for 1 hour b. 120o C for 1 hour c. 160o C for 1 hour d. 60o C for 1 hour
33. Phenol co-efficient indicates
a. Efficiency of a disinfectant b. Dilution of a disinfectant c. Purity of a disinfectant d. Quantity
of a disinfectant
34. This is an agar plate method and is commonly used for estimation of the number of bacteria
in milk.
a. Standard Plate Count (SPC) b. Spread plate c. Lawn culture d. Roll tube method
35. Agar is obtained form
a. Brown algae b. Red algae c. Green algae d. Blue-green algae
36. A gram positive organism which produces swarming on culture medium is
a. Salmonella b. Clostridium c. Staphylococci d. Proteus
37. Enhancement of virulence in bacteria is known as
a. Pathogenicity b. Attenuation c. Exaltation d. Toxigenicity
38. For effective sterilization in an autoclave the temperature obtained is
a. 50o C b. 100o C c. 120o C d. 180o C
39. Spores are killed by
a. 70% alcohol b. Glutaraldehyde c. Autoclaving d. Both b and c
40. Glassware are sterilized by
a. Autoclaving b. Hot air over c. Incineration d. None of these
41. Tyndallisation was proposed by a. Tyndall b. Pasteur c. Koch d. Jenner
42. Viruses can be cultivated in
a. Lab media b. Broth c. Living cells d. None of these
43. By pasteurization
a. All the microorganisms can be removed b. Only pathogenic forms can be removed c. Only
non-pathogenic forms can be removed d. All of these are correct
44. The temperature required for pasteuriza- tion is
a. Above 100o C b. Below 100o C c. 100o C d. None of these
45. In the medium other than nutrients, if any substance is used in excess, that medium is
a. Enriched medium b. Special medium c. Enrichment medium d. None of these
46. Example for indicator medium is
a. Nutrient Agar b. Nutrient broth c. Wilson and Blair d. Czapeck-dox medium
47. Example of Anaerobic medium is
a. Robertson cooked-meat medium b. Nutrient agar c. Nutrient broth d. Mac-Conkey’s agar
48. The differentiate lactose and non-lactose fermentors, the medium used is
a. Wilson lair b. Blood Agar c. Tetra thionate broth d. Mac-Conkey’s Agar
49. Best method for getting pure culture is
a. Streak-plate b. Agar slant c. Both a b d. None of these
50. To transfer cultures from one place to another, the device used is
a. Slant b. Needle c. Inoculation loop d. Autoclave
51. The bacterial culture prepared by pure culture is
a. Inoculum b. Suspension c. Dilution d. None of these
52. Separation of a single colony is
a. Pure-culturing b. Isolation c. Separation d. Both a and b
53. Growth period of the culture is
a. Inoculation b. Incubation c. Incineration d. Isolation
54. At the temperature 160o C for one hour, complete sterilization occurs in
a. Autoclave b. Hot air oven c. Laminar flow d. Incubator
55. In autoclave, the principle involved is a. Dry heat b. Moist heat c. Steam under pressur d.
Both b and c
56. The spores of th bacteria which can withstand the moist heat effect also
a. Bacillus subtilis b. Coxiella burnetti c. Bacillus stearothermophilus d. Pseudomonas
57. Factors on which disinfectivity of a disinfectant depends
a. Concentration of the substance b. Time of action c. pH of the medium and temperature
suitable for the chemical d. All of the above
58. Aldehydes, which are most powerful disinfectants
a. Formaldehyde b. Acetaldehyde c. Glutamal aldehyde d. Both a and c
59. Accridine dyes are more effective against
a. Gram positive b. Gram negative c. Mycoplasmas d. Rickttsiae
60. The sterilizing agent is
a. Ethelene oxide b. Oxygen c. Nitrogen d. Carbon tetrachloride
61. Salts of heavy metals used as disinfec- tants are
a. Thiomersal b. Phenyl mercury nitrate c. Mercurochrome d. All of these
62. Cultures are prepared by penetrating the inoculation loop with suspension into the
medium, they are
a. Stock cultures b. Stabcultures c. Sub-cultures d. None of these
63. The principle involved in the streak plate method is
a. Separation b. Streaking c. Isolation d. Dilution
64. Culture media for fungi are
a. Potato dextrose agar (PDA) b. Sabouraud’s agar c. Czapekdox agar d. All of the above
65. Spores of actinomycetes are very sensitive, killed at room temperature of
a. 52o C for 30 min. b. 65o C for 30 min. c. 70o C for 30 min. d. 43o C for 30 min.
66. The term that is used for the bacteria which can withstand pasteurization but does not grow
at higher temperatures
a. Thermophiles b. Extreme thermophiles c. Thermoduric d. Facultative thermophiles
67. A common laboratory method of cultivating anaerobic micro-organisms is
a. Gas pack system b. Brewer jar system c. Pyrogallic acid over the cotton d. None of these
68. Alkaliphiles grow at pH value between
a. 1 to 6 b. 6 to 9 c. 1 to 11 d. 7 to 12
69. The micro-organisms grow at high salinity are
a. Osmophiles b. Halophiles c. Both a and b d. None of these
70. Non-lactose fermenting colonies seen on Mac Conkey’s medium are
a. Salmonella typhi b. Escherichia coli c. Klebsiella pneumoniae d. Shigella shigae
71. Wilson and Blair medium is used for isolation of
a. Staphylococci b. Salmonella typhosa c. Vibrio cholerae d. Shigella shigae
72. Laboratory diagnosis of enteric fever is based on
a. Blood culture b. Urine and stool culture c. Widal test d. All of the above
73. Shigella was first isolated by
a. Shiga b. Schmitz c. Sonnei d. Robert Koch
74. Which of the following are gas producing Salmonella? a. S.typhi b. S.enteritidis c.
S.cholerasuis d. S.typhimurium
75. Kauffmann white scheme is used to detect
a. Salmonella spp. b. Shigella spp. c. E.coli d. None of these
76. On Mac Conkey’s medium Esch. Coli forms
a. Colorless colonies b. Greenish pigmentation c. Pink coloured colonies d. Medusa head
appearance
77. C.diphtheriae requires
a. LJ medium b. Mac Conkey’s medium c. Potassium tellurite medium d. PDA medium
78. Culture medium for Mycobacterium tuberculosis
a. LJ medium b. Mac Conkey’s medium c. Wilson blair medium d. None of these
79. Lepra bacillus is best cultured on
a. Armadillo’s brain b. Foot pad of mice c. Liver of guinea pig d. Any of the above
80. Culture medium for clostridia spp.
a. 76 Lower stein Jensen’s medium b. Mac Conkey’s medium c. Robertson’s cooked meat
medium d. None of these
81. Clsotridium welchii is positive for
a. Elek’s gel precipitation test b. Nagler’s test c. Weil felix test d. Bacitracin test
82. Nagler’s reaction detects
a. Coagulase b. Hyaluronidase c. Lecithinase d. None of these
83. Incubation period of Cl. welchii is
a. 8-12 hours b. 7-10 hours c. 5-7 hours d. 2-4 hours
84. The average incubation period of tetanus is
a. 2-3 days b. 7-10 days c. 14-21 days d. 3-4 weeks
85. Salt agar is used for
a. Streptococcus b. Staphylococcusc. Vibrio d. Shigella
86. Culture medium of Leishmania is
a. Sabousand’s medium b. NNN medium c. Wilson Blair medium d. Czapek – dox medium
87. A simple asexual spore which develops by budding is known as
a. Chlamydospore b. Blastospore c. Arthospore d. Conidia
88. Culture medium used for fungus is a. Sabouraud’s medium b. Nutrient agar c. Nutrient
broth d. Minimal agar medium
89. For sterilization of fermentation equipment the method followed is
a. Radiation b. Chemicals c. Heating d. All of these
90. Listed below are substances which are assayed by organisms mentioned in A to E. Match
them correctly:
1. Crystal Violet I.P. A. Pasteurella pestis 2. Ampicillin I.P. B. Bacillus cerus 3. Plaque Vaccine I.P.
C. Micrococcus luteus 4. Rifampicin D. Lactobacillus aureus E. Lactobacillus aureus F. Bacillus
subtillus
91. Match the following terms with their respective formulations A to E:
1. Lysol A. Higher boiling fractions of the tar acids 2. Black fluids B. Prepared from refined tar
acids 3. White fluids C. Solution of cresol with soap 4. Iodophores D. Basic molecules has
varying numbers of amino groups E. Iodine combined with complex organic chemicals
92. Match the following tests with their respective applications A to E:
1. Schick test A. Tuberculosis 2. Mantoux test B. Detection of extraneous microorganisms 3.
Sterility test C. Diphtheria toxin 4. Potency test D. Detection of infection caused by Rickettsia
prowazeki E. Usefulness of immunological products
93. Match the following equipments with their respective methods of sterilization A to E:
1. Glass syringes A. Autoclave 2. Disposable B. Chemical instrument 3. Respiratory parts C. Dry
heat 4. Dialysis machine D. g-Radiation E. Chicken pox in children
94. The items listed from A to D can be identified by the tests given below :
1. Coomb’s test A. Candida albicans 2. Coagulase test B. Virulent staphylococcus aureus C.
Mycobacterium tuberculosis D. Non-agglutinating antibodies
95. D.pneumoniae can be cultivated in
a. Glucose broth b. Serum broth c. Agar and blood agar d. Chocolate agar e. All of these
96. D.pneumoniae can be identified by
a. Microscopic exam b. Culture of sputum/blood c. Animal inoculation d. All of these e. None of
these
97. The diagnosis of tuberculosis is carried out by
a. Emulator b. Antiformin method c. Petroff’s method d. Concentration method e. All of these
98. The size of the virus can be determined by
a. Micrography b. Ultra-centrifugation at high speed c. Ultra-filteration d. All of these
99. Differential staining of bacteria spore is related to
a. Albert’s staining b. Lugol’ s staining c. Moller’s staining d. Indian ink preparation
100. Electron microscope studies does not help in identifying the section of bacterial spore
a. Core b. Spore cortex c. Capsule d. All of these
101. Wilson and Blair bismuth sulphite medium is used for the growth
a. Salmonella typhi b. Shigella dysenteriae c. Vibrio cholerae d. E. coli
102. Which Rickettsia can be grown on blood agar media?
a. Lactobacilli b. Streptobacillus c. Bacillus anthrax d. Vibrio cholerae
ANSWERS 1. b 2. b 3. c 4. b 5. d 6. a 7. b 8. a 9. c 10. b 11. a 12. c 13. b 14. c 15. a 16. b 17. c 18.
a 19. a 20. d 21. a 22. b 23. b 24. c 25. d 26. c 27. c 28. b 29. b 30. d 31. b 32. c 33. a 34. a 35. b
36. d 37. c 38. c 39. d 40. b 41. a 42. c 43. b 44. b 45. a 46. c 47. a 48. d 49. c 50. b 51. a 52. b
53. b 54. b 55. d 56. c 57. d 58. d 59. a 60. a 61. d 62. b 63. d 64. d 65. b 66. c 67. c 68. d 69. c
70. a 71. b 72. d 73. c 74. b 75. a 76. c 77. c 78. a 79. b 80. c 81. b 82. c 83. a 84. b 85. b 86. b
87. b 88. b 89. d 90. 1.d, 2.c, 3.a, 4.e 91. 1.c, 2.a, 3.b, 4.e 92. 1.c, 2.a, 3.b, 4.e 93. 1.c, 2.d, 3.e,
4.b 94. 1.d, 2.a 95. e 96. e 97. e 98. d 99. c 100. c 101. a 102. a
